##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936463_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1576763 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.907721071587805 31.0 31.0 34.0 30.0 34.0 2 32.02851665088539 33.0 31.0 34.0 30.0 34.0 3 32.161571523431235 33.0 31.0 34.0 30.0 34.0 4 35.87896912852471 37.0 35.0 37.0 35.0 37.0 5 35.738617027416296 37.0 35.0 37.0 35.0 37.0 6 35.789709043147255 37.0 35.0 37.0 33.0 37.0 7 35.72407013609528 37.0 35.0 37.0 33.0 37.0 8 35.69060220210647 37.0 35.0 37.0 33.0 37.0 9 37.30474078856493 39.0 37.0 39.0 34.0 39.0 10-11 37.2035775192594 39.0 37.0 39.0 33.0 39.0 12-13 37.08502768012694 39.0 37.0 39.0 33.0 39.0 14-15 38.17116903428099 40.0 37.5 41.0 33.0 41.0 16-17 38.15793749599655 40.0 37.5 41.0 33.0 41.0 18-19 38.30879276086514 40.0 38.0 41.0 33.5 41.0 20-21 38.31508286280183 40.0 38.0 41.0 34.0 41.0 22-23 38.22030672967338 40.0 38.0 41.0 33.0 41.0 24-25 38.09447139487672 40.0 37.0 41.0 33.0 41.0 26-27 37.88820196820956 40.0 37.0 41.0 33.0 41.0 28-29 37.93509487475289 40.0 37.0 41.0 33.0 41.0 30-31 37.85037859209025 40.0 37.0 41.0 33.0 41.0 32-33 37.676003305506285 40.0 37.0 41.0 33.0 41.0 34-35 37.486379373437856 40.0 36.0 41.0 32.0 41.0 36-37 37.31781123732609 39.0 36.0 41.0 31.5 41.0 38-39 37.12022954622857 39.0 36.0 41.0 31.0 41.0 40-41 36.943783244533265 39.0 36.0 41.0 31.0 41.0 42-43 36.69623938410528 39.0 35.5 40.0 30.5 41.0 44-45 36.450697092714634 39.0 35.0 40.0 30.0 41.0 46-47 36.13336563579942 39.0 35.0 40.0 30.0 41.0 48-49 35.76663392025307 38.0 35.0 40.0 28.5 41.0 50-51 35.491887493554835 38.0 35.0 40.0 28.0 41.0 52-53 35.24408994883822 38.0 34.0 40.0 27.0 41.0 54-55 34.93699814112838 38.0 34.0 40.0 26.5 41.0 56-57 34.586198116013634 38.0 33.5 40.0 26.0 41.0 58-59 34.214415229175216 37.0 33.0 40.0 25.5 41.0 60-61 33.79725741915557 37.0 33.0 39.5 24.5 40.5 62-63 33.48207181421685 36.0 32.0 39.0 23.5 40.0 64-65 33.17659058463447 36.0 32.0 39.0 23.0 40.0 66-67 32.71530217286936 35.5 31.0 39.0 22.0 40.0 68-69 32.23637794646373 35.0 31.0 38.0 21.5 40.0 70-71 31.69538510226331 35.0 30.5 37.5 19.5 39.5 72-73 31.09537926752467 34.0 30.0 37.0 18.0 39.0 74-75 30.567500949730555 34.0 29.0 36.0 16.5 39.0 76-77 29.20366155218 32.5 28.0 34.5 14.0 37.0 78-79 30.003900395937755 34.0 29.0 35.0 14.0 37.0 80-81 29.80699699320697 34.0 29.0 35.0 10.5 37.0 82-83 29.367652272408726 34.0 29.0 35.0 7.0 36.5 84-85 28.88792608654566 33.0 29.0 35.0 4.5 36.0 86-87 28.489619873119803 33.0 28.0 35.0 2.0 36.0 88-89 27.977131629801054 33.0 27.0 35.0 2.0 35.0 90-91 27.358096302361233 32.0 26.0 34.0 2.0 35.0 92-93 26.730050426094472 32.0 25.0 34.0 2.0 35.0 94-95 25.94322735883579 32.0 24.5 34.0 2.0 35.0 96-97 25.057496909808258 31.0 21.0 34.0 2.0 35.0 98-99 23.841851311833167 31.0 8.5 34.0 2.0 35.0 100-101 19.961276361761406 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24441995722522236 1101 2 0.418901687121469 1101 3 0.293959806867349 1101 4 0.2151361942191059 1101 5 0.25905594845896474 1101 6 0.21329763104832722 1101 7 0.26645259389540144 1101 8 0.19163738445241307 1101 9 0.2984066471708928 1101 10-11 0.2874228068578404 1101 12-13 0.25847740472890024 1101 14-15 0.35168279142768455 1101 16-17 0.37269196811234195 1101 18-19 0.23932565262046523 1101 20-21 0.26704348564856417 1101 22-23 0.24760203217132215 1101 24-25 0.3842374760883871 1101 26-27 0.31119070026962703 1101 28-29 0.324882536025207 1101 30-31 0.3396141362714431 1101 32-33 0.39745037218526136 1101 34-35 0.3573605732562797 1101 36-37 0.421470467158791 1101 38-39 0.429082182496451 1101 40-41 0.2647863511624493 1101 42-43 0.35443771742768604 1101 44-45 0.38246876789634854 1101 46-47 0.24259207998717613 1101 48-49 0.34694567558445755 1101 50-51 0.43176841487473894 1101 52-53 0.4164342147168796 1101 54-55 0.43521174376837024 1101 56-57 0.3251106837177673 1101 58-59 0.4970974379135811 1101 60-61 0.5507004166301996 1101 62-63 0.4030287775389283 1101 64-65 0.28919380835645114 1101 66-67 0.32497241309044256 1101 68-69 0.35942337504241806 1101 70-71 0.2376053732040866 1101 72-73 0.3845294933642478 1101 74-75 0.465131698877979 1101 76-77 0.5890742984141433 1101 78-79 0.5957051186387226 1101 80-81 0.7852816847159083 1101 82-83 0.5341219397331436 1101 84-85 0.46116135062228025 1101 86-87 0.49282774146624675 1101 88-89 0.5743054293068397 1101 90-91 0.6817574439759078 1101 92-93 0.6149901210343813 1101 94-95 0.5828451204522445 1101 96-97 0.554985047964486 1101 98-99 -0.07381805498757288 1101 100-101 0.2178346164608591 1102 1 0.23200491870329998 1102 2 0.39291844801332587 1102 3 0.28359359907585 1102 4 0.21006890563592862 1102 5 0.07377640364376958 1102 6 0.06744429565807053 1102 7 0.15438329981298438 1102 8 0.11853988633539814 1102 9 0.23321680534216682 1102 10-11 0.25074746633133316 1102 12-13 0.3212922953339117 1102 14-15 0.37866605775980844 1102 16-17 0.4614357633154782 1102 18-19 0.375260274252625 1102 20-21 0.38469369391194874 1102 22-23 0.3612464598780676 1102 24-25 0.2597272807110187 1102 26-27 0.30907675887376485 1102 28-29 0.430176691038497 1102 30-31 0.5303222544597759 1102 32-33 0.46726420559270565 1102 34-35 0.42764549480586567 1102 36-37 0.5616094909071663 1102 38-39 0.48001017962886294 1102 40-41 0.5807208820480483 1102 42-43 0.6033638451807164 1102 44-45 0.7292698377984337 1102 46-47 0.6953021129281822 1102 48-49 0.7235264808695376 1102 50-51 0.5931488290420859 1102 52-53 0.5656582180926506 1102 54-55 0.6867058860771422 1102 56-57 0.6202889780451954 1102 58-59 0.6206425434972118 1102 60-61 0.5961911060319096 1102 62-63 0.6572163915393148 1102 64-65 0.5927005962000393 1102 66-67 0.7731532846205198 1102 68-69 0.6476460580555639 1102 70-71 0.30001621469704887 1102 72-73 0.25835704765338363 1102 74-75 0.3639492710441914 1102 76-77 0.3966941746358188 1102 78-79 0.44236439702197927 1102 80-81 0.5226702932987202 1102 82-83 0.6457536084718534 1102 84-85 0.402823669015703 1102 86-87 0.5790771402702077 1102 88-89 0.3637318655982078 1102 90-91 0.5402074244653967 1102 92-93 0.6351092032235535 1102 94-95 0.6269592977891953 1102 96-97 0.33014861866060485 1102 98-99 0.2934419430160027 1102 100-101 0.13485629127959342 1103 1 0.25771640620186176 1103 2 0.22237556672899927 1103 3 0.2650152298320805 1103 4 0.15576925513855855 1103 5 0.22915912822801232 1103 6 0.19734213335058826 1103 7 0.1888592379010916 1103 8 0.2343798448559653 1103 9 0.184732917029379 1103 10-11 0.2776846134680966 1103 12-13 0.2929504164692176 1103 14-15 0.4330031549676221 1103 16-17 0.49124142448996366 1103 18-19 0.37160729963957806 1103 20-21 0.27231035481758425 1103 22-23 0.40352763666493985 1103 24-25 0.3371267506960365 1103 26-27 0.48871535010930955 1103 28-29 0.3418312595371731 1103 30-31 0.5378332004212965 1103 32-33 0.4721610831131571 1103 34-35 0.42174667773785046 1103 36-37 0.47881961496140235 1103 38-39 0.23744143130916484 1103 40-41 0.35558213718496745 1103 42-43 0.5142488357710207 1103 44-45 0.6914481801573871 1103 46-47 0.6516426091450995 1103 48-49 0.6372098929369798 1103 50-51 0.6505896824430195 1103 52-53 0.560275973594436 1103 54-55 0.4860241639125391 1103 56-57 0.476438400021614 1103 58-59 0.468339090718473 1103 60-61 0.2917258258536961 1103 62-63 0.6880604787997484 1103 64-65 0.6797099482759279 1103 66-67 0.4057612079603814 1103 68-69 0.5755928261163987 1103 70-71 0.480650984447788 1103 72-73 0.7283670334428365 1103 74-75 0.7889588727648196 1103 76-77 0.4999774830046775 1103 78-79 0.3882034913876886 1103 80-81 0.6981297356307827 1103 82-83 0.5230445790137743 1103 84-85 0.5198249556462677 1103 86-87 0.6143538618587172 1103 88-89 0.4403199901901367 1103 90-91 0.47055894345759697 1103 92-93 0.6426268703895097 1103 94-95 0.5644463354663465 1103 96-97 0.8667090902971211 1103 98-99 0.6758323873836325 1103 100-101 0.42490170741816513 1104 1 0.2050969917180403 1104 2 0.24547092711249974 1104 3 0.19311829437582162 1104 4 0.22894165712452264 1104 5 0.04805144118812166 1104 6 0.1018191064716234 1104 7 0.10651888522175312 1104 8 0.04537111347435285 1104 9 0.08415082492186343 1104 10-11 0.10431532738694926 1104 12-13 0.08341865820865024 1104 14-15 0.173980727301128 1104 16-17 0.11636554707171598 1104 18-19 0.16068879253462853 1104 20-21 0.21241050890078128 1104 22-23 -0.0027812145591568083 1104 24-25 0.019458543197323763 1104 26-27 0.10128169148921273 1104 28-29 0.11417332425212123 1104 30-31 0.1460124505496978 1104 32-33 0.11025815553226437 1104 34-35 0.23337139953206787 1104 36-37 0.13569343793691502 1104 38-39 0.22543150150261937 1104 40-41 0.14226250013651764 1104 42-43 0.04680747299067178 1104 44-45 0.11812597505551992 1104 46-47 -0.0033864954602691455 1104 48-49 0.08056719476885377 1104 50-51 0.12904799437590952 1104 52-53 0.21394828997861737 1104 54-55 0.1079125391907425 1104 56-57 0.3702707917353578 1104 58-59 0.13677343415282195 1104 60-61 -0.021914817871682146 1104 62-63 0.012799393369171241 1104 64-65 -6.016422051544623E-4 1104 66-67 0.1237547022250638 1104 68-69 0.1659497855411587 1104 70-71 0.026212532127981802 1104 72-73 0.16933176550926277 1104 74-75 -0.039011509019122315 1104 76-77 -0.012841383627748115 1104 78-79 0.15483163725312465 1104 80-81 0.22573630984753024 1104 82-83 0.26791703236080266 1104 84-85 0.264384962494411 1104 86-87 0.23374694523485218 1104 88-89 0.34525921292596706 1104 90-91 0.3390960090285624 1104 92-93 0.46870117273499545 1104 94-95 0.3910513688510413 1104 96-97 0.5629040911531398 1104 98-99 0.6580015365019314 1104 100-101 0.4677796392791471 1105 1 0.18444130996259034 1105 2 0.27347943933124697 1105 3 0.23159141513790615 1105 4 0.11285476825013063 1105 5 0.0702228600083572 1105 6 0.15927909251881545 1105 7 0.15038491815641208 1105 8 0.15590782827320027 1105 9 0.10515867296002313 1105 10-11 0.2198768945854539 1105 12-13 0.24369154107027668 1105 14-15 0.3111461484200646 1105 16-17 0.26270360328113185 1105 18-19 0.2500850741271279 1105 20-21 0.21684939341199794 1105 22-23 0.12513294051780832 1105 24-25 0.17147183015512724 1105 26-27 0.30858341183405713 1105 28-29 0.14227050541616393 1105 30-31 0.28594543021104357 1105 32-33 0.36305290030790616 1105 34-35 0.16336810372410326 1105 36-37 0.3013906017017618 1105 38-39 0.3306635548244472 1105 40-41 0.33471384514403724 1105 42-43 0.36922448805938046 1105 44-45 0.33605960384047506 1105 46-47 0.5052001717417482 1105 48-49 0.4106758225918341 1105 50-51 0.7257887414368653 1105 52-53 0.8102858442883445 1105 54-55 0.5414760771820326 1105 56-57 0.764461135519916 1105 58-59 0.7852780240254873 1105 60-61 0.570139440830836 1105 62-63 0.5523762752002384 1105 64-65 0.4616630295775437 1105 66-67 0.536001723868651 1105 68-69 0.6141650302423542 1105 70-71 0.38017143323533986 1105 72-73 0.5720736435175269 1105 74-75 0.4905340710012531 1105 76-77 0.46187249172379197 1105 78-79 0.488008545182975 1105 80-81 0.4614304956742181 1105 82-83 0.6399020790960321 1105 84-85 0.5773604078885626 1105 86-87 0.5241891857819887 1105 88-89 0.46315419543476466 1105 90-91 0.43958552852652844 1105 92-93 0.477903085307414 1105 94-95 0.410236484513824 1105 96-97 0.4244584075741251 1105 98-99 0.6363759335949304 1105 100-101 0.23085538096141534 1106 1 0.07098865706534951 1106 2 0.21171364146853477 1106 3 0.15748824748757784 1106 4 0.10120111443902857 1106 5 -0.03684255054738372 1106 6 0.057010972216502864 1106 7 0.15629298015235094 1106 8 0.04342481448487945 1106 9 0.11592864749518128 1106 10-11 0.07198726944958622 1106 12-13 0.08834611971163753 1106 14-15 0.13044897759592544 1106 16-17 0.18399518330210896 1106 18-19 0.17069857738049166 1106 20-21 0.09926533414482464 1106 22-23 0.02991078312108897 1106 24-25 0.11921206197843048 1106 26-27 -0.049275450655208886 1106 28-29 0.09154201107274673 1106 30-31 0.16954971991153656 1106 32-33 -0.020350359215804303 1106 34-35 -0.018873448283734717 1106 36-37 0.20669182385550045 1106 38-39 0.15910982779507066 1106 40-41 0.13841892732102679 1106 42-43 0.16948919557342634 1106 44-45 0.31021175640271537 1106 46-47 0.30977658789952045 1106 48-49 0.26557013772058014 1106 50-51 0.1370563990192295 1106 52-53 0.18964320004194946 1106 54-55 0.2879402932009256 1106 56-57 0.1624173642852469 1106 58-59 0.12344248286566994 1106 60-61 0.030661930300759366 1106 62-63 -0.048781835414828834 1106 64-65 -0.004809587338492349 1106 66-67 -0.054863001568932646 1106 68-69 -0.011282524810042105 1106 70-71 -0.028137773428561275 1106 72-73 0.007748864102040898 1106 74-75 -0.05837082511232339 1106 76-77 -0.17725029732529407 1106 78-79 -0.2695411362474509 1106 80-81 -0.02005293328181068 1106 82-83 -0.08398472582574712 1106 84-85 -0.035055545037153024 1106 86-87 -0.015200383571443155 1106 88-89 -0.007284919093606845 1106 90-91 0.1620038529682013 1106 92-93 -0.02751985231195775 1106 94-95 0.0691015342821295 1106 96-97 0.04821831505451968 1106 98-99 0.07400643555768127 1106 100-101 0.14285354471306633 1107 1 0.1170127595660091 1107 2 0.2242588059003765 1107 3 0.11746344075486803 1107 4 0.05462219127963408 1107 5 0.095098179760285 1107 6 0.1632983052389605 1107 7 0.04416654829314837 1107 8 -0.0046545670598021616 1107 9 0.014402494156584567 1107 10-11 0.011377474279598232 1107 12-13 0.14094305471609658 1107 14-15 0.23425294096316662 1107 16-17 0.1160060195935344 1107 18-19 0.22676480691572465 1107 20-21 0.051367879214090806 1107 22-23 0.14875961749014976 1107 24-25 0.1366901816153998 1107 26-27 0.19581173771417326 1107 28-29 0.2855138481350181 1107 30-31 0.27980803349782235 1107 32-33 0.35679128342132316 1107 34-35 0.36647360805800844 1107 36-37 0.38926303218447345 1107 38-39 0.4666487588215702 1107 40-41 0.5006600348052359 1107 42-43 0.5486821519840035 1107 44-45 0.48877312451622856 1107 46-47 0.4853654305797903 1107 48-49 0.4222467017025977 1107 50-51 0.3968702850795509 1107 52-53 0.3575281564398338 1107 54-55 0.33836965269870234 1107 56-57 0.439428470215077 1107 58-59 0.4125310099103956 1107 60-61 0.31545035932399657 1107 62-63 -0.08165361125332282 1107 64-65 0.1095678493202783 1107 66-67 0.3057269672481766 1107 68-69 0.3666945210316612 1107 70-71 0.22919147408997276 1107 72-73 0.3928294542611894 1107 74-75 0.19784005749346179 1107 76-77 0.023445211133424237 1107 78-79 0.2296903943152735 1107 80-81 0.24473990512231225 1107 82-83 0.47585231741473777 1107 84-85 0.40408706829821384 1107 86-87 0.6442502837042916 1107 88-89 0.5003696392228356 1107 90-91 0.3097174779551146 1107 92-93 0.25162361752184736 1107 94-95 0.3207123858001957 1107 96-97 0.7990622832435363 1107 98-99 1.1368921374264325 1107 100-101 0.902396999320235 1108 1 0.02587788677944758 1108 2 0.22480930003521848 1108 3 0.018221792099041068 1108 4 0.04766810945351807 1108 5 0.12805750799887505 1108 6 0.050202956345636096 1108 7 0.07764982836953038 1108 8 0.1288276789011178 1108 9 0.06375806786660831 1108 10-11 0.15717413960192061 1108 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-1.324405705582656 2316 54-55 -1.174002710334527 2316 56-57 -1.1406290636054877 2316 58-59 -1.6238604273984691 2316 60-61 -1.1245771117795087 2316 62-63 -1.0138207184942942 2316 64-65 -1.0258595077522372 2316 66-67 -1.2353656570196918 2316 68-69 -1.2555233818346636 2316 70-71 -1.1122898922086826 2316 72-73 -0.8820189363468032 2316 74-75 -1.0613446208872972 2316 76-77 -1.104287645343021 2316 78-79 -1.4975137729993016 2316 80-81 -1.3167020686505602 2316 82-83 -1.4666320033233582 2316 84-85 -1.2464131131147376 2316 86-87 -1.5108500117396524 2316 88-89 -1.525183670267655 2316 90-91 -1.792671989727463 2316 92-93 -1.5976196313248856 2316 94-95 -1.9062792692042123 2316 96-97 -1.5830244839777379 2316 98-99 -0.8675602546328633 2316 100-101 -0.8479321355991623 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 21.0 5 48.0 6 80.0 7 216.0 8 530.0 9 1076.0 10 1696.0 11 2618.0 12 3784.0 13 5308.0 14 6947.0 15 9128.0 16 11091.0 17 13744.0 18 12855.0 19 10070.0 20 10045.0 21 10603.0 22 11449.0 23 12931.0 24 14813.0 25 18268.0 26 22151.0 27 27553.0 28 33795.0 29 41380.0 30 51065.0 31 63188.0 32 78954.0 33 100951.0 34 132249.0 35 177949.0 36 247037.0 37 295585.0 38 143021.0 39 4556.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.441760159940735 19.290567454699527 15.39194866876741 22.87572371659233 2 15.527431459408442 20.85394752198424 39.339737051752394 24.278883966854924 3 20.735773438000457 23.38336036268592 30.212516038437702 25.668350160875914 4 13.086083542253446 17.130138467578114 38.08901677428193 31.694761215886512 5 13.567812423493237 37.392002577604025 34.795814945283 14.244370053619745 6 31.558896295765436 36.7687471103774 16.985241282297974 14.687115311559188 7 27.856599700271634 32.79101905742215 20.82598440727542 18.526396835030802 8 25.521979224208952 34.217521098926326 19.707510247415712 20.55298942944901 9 24.710862781907203 17.87627793703544 21.16878916258657 36.24407011847079 10-11 25.0882979877128 26.054581443121126 27.440585554075025 21.416535015091043 12-13 26.528590536434454 23.53920658970308 27.497791361162076 22.434411512700386 14-15 22.62674725386235 25.345899185671882 26.93406098581902 25.093292574646743 16-17 21.747820908245064 28.41854037162687 27.562252289449603 22.271386430678465 18-19 21.456135160272602 27.968102449649923 29.10649883889847 21.469263551179 20-21 22.646145181810226 26.10530875456793 28.917129748838438 22.331416314783407 22-23 21.808390711178745 26.183958134282708 29.26591979542802 22.741731359110528 24-25 22.779755288939484 26.10607528424631 29.115701051049385 21.99846837576482 26-27 22.26485886727883 25.921533422482362 30.04416333361978 21.769444376619024 28-29 21.469702298033024 27.527276636320714 29.25585131296014 21.747169752686123 30-31 22.040318921124957 26.43720150296162 28.83535695338555 22.687122622527873 32-33 22.03322107426223 26.414686277121348 29.108523444387362 22.443569204229057 34-35 22.969504072138193 26.2288001628665 28.88292796930905 21.918767795686257 36-37 22.610127052723104 25.87019350599684 29.682694814069727 21.836984627210327 38-39 22.171415487812304 26.70390544133466 29.353759057051814 21.77092001380122 40-41 22.267614092923285 26.999713970964567 28.886395736074476 21.84627620003767 42-43 21.928969198126385 26.915084524161742 28.182288946454975 22.973657331256895 44-45 22.48870498762757 26.298454707214443 28.32849672875131 22.88434357640667 46-47 23.234595180125357 26.976755542843158 28.055991927765934 21.73265734926555 48-49 22.908795014487275 26.842224263720738 28.102491963581556 22.146488758210435 50-51 21.889012208657046 27.19971460282226 28.940510543840176 21.970762644680512 52-53 22.732095642120232 27.608128857214798 27.459817739366915 22.19995776129806 54-55 22.056333785208615 26.886896409573414 28.045060686057667 23.011709119160304 56-57 22.943824400986433 26.59629124085369 27.697527324761257 22.762357033398622 58-59 23.616849091091286 26.543070895006487 27.583264308446743 22.256815705455484 60-61 22.6952671128272 26.79887005363059 28.810588109940177 21.69527472360203 62-63 22.117126258910677 27.081864075699535 28.573276846190925 22.227732819198863 64-65 21.936109306190712 27.814526274197167 27.893012563831554 22.35635185578057 66-67 22.37480410642404 26.91592003546538 27.42716183742625 23.282114020684332 68-69 22.295073025742695 26.861048931068787 27.863529684852622 22.980348358335895 70-71 23.17671368934588 26.629874305981026 27.530402375615694 22.663009629057402 72-73 22.69458115581714 26.32490232888528 28.577902227408796 22.40261428788878 74-75 22.039836604530258 27.794671729672526 27.627863995248198 22.537627670549014 76-77 22.300939116757757 26.884742167024207 27.51480500206913 23.299513714148905 78-79 22.530352098293736 26.99075642084353 27.70505028978944 22.773841191073295 80-81 23.102489719189915 26.73701928809354 27.35475581014813 22.805735182568416 82-83 22.81701610560671 26.602379119157643 28.206692636222837 22.373912139012813 84-85 22.40512831896292 27.258543762902455 27.899329944473866 22.43699797366076 86-87 22.25872481572092 27.506377824040236 27.42034656675667 22.814550793482173 88-89 22.00960913079891 27.42981595765158 26.901031533944153 23.659543377605353 90-91 22.45895301935098 26.632611089986703 27.291195794089766 23.61724009657255 92-93 22.940608652855786 26.876417492427358 27.595501570340602 22.587472284376254 94-95 22.573614610729983 26.753961765875445 27.68967863827002 22.98274498512455 96-97 21.59347980641352 26.835929052115 28.610609203792837 22.95998193767865 98-99 22.057333592515512 27.32835073564084 27.17854935565429 23.435766316189362 100-101 21.591711960830327 26.752582877220576 27.44353820882459 24.212166953124502 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 108.0 1 85.5 2 78.5 3 97.0 4 83.0 5 84.5 6 153.5 7 313.5 8 557.0 9 719.5 10 673.5 11 590.5 12 670.0 13 777.0 14 856.5 15 1000.5 16 1177.5 17 1351.0 18 1531.5 19 1794.5 20 2102.5 21 2488.0 22 2989.5 23 3590.0 24 4458.5 25 5735.5 26 7472.5 27 9719.0 28 12092.0 29 14977.5 30 18211.5 31 21471.0 32 25197.5 33 28164.0 34 30812.0 35 33895.0 36 37481.0 37 40666.5 38 42965.5 39 44920.0 40 46936.0 41 49076.0 42 50851.0 43 52745.5 44 54460.0 45 56398.5 46 58876.5 47 70404.0 48 91199.5 49 87518.0 50 65392.0 51 55342.0 52 52520.0 53 50763.5 54 47933.0 55 43970.5 56 39501.5 57 35133.0 58 32008.5 59 28402.0 60 23321.5 61 18938.5 62 15518.5 63 11721.5 64 8114.0 65 5953.0 66 4540.0 67 3649.5 68 2955.0 69 2382.0 70 1915.0 71 1435.0 72 1087.5 73 890.0 74 775.5 75 660.5 76 489.0 77 300.5 78 202.0 79 146.5 80 97.5 81 69.0 82 45.0 83 30.5 84 17.5 85 6.5 86 4.0 87 3.5 88 3.0 89 1.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011352371916388194 2 0.011352371916388194 3 0.005200527917004648 4 2.536842886343731E-4 5 0.006659212576652293 6 0.0 7 1.2684214431718654E-4 8 0.0015221057318062384 9 1.902632164757798E-4 10-11 0.0 12-13 0.0 14-15 1.902632164757798E-4 16-17 0.004693159339735902 18-19 0.0017440794843613147 20-21 5.707896494273394E-4 22-23 4.7565804118944953E-4 24-25 3.4881589687226297E-4 26-27 1.5855268039648317E-4 28-29 9.51316082378899E-5 30-31 6.02500185506636E-4 32-33 0.0033613168244054437 34-35 7.293423298238227E-4 36-37 0.0010147371545374923 38-39 0.005771317566431988 40-41 0.0 42-43 0.0015538162678855351 44-45 0.004534606659339418 46-47 0.0 48-49 0.002188026989471468 50-51 8.244739380617126E-4 52-53 6.342107215859327E-4 54-55 3.1710536079296635E-5 56-57 0.01084500333911945 58-59 0.002346579669867951 60-61 0.0032344746800882567 62-63 1.902632164757798E-4 64-65 0.003456448432643333 66-67 8.878950102203057E-4 68-69 0.0015538162678855351 70-71 0.004883422556211682 72-73 0.0027271061028195107 74-75 0.006183554535462843 76-77 9.830266184581958E-4 78-79 0.00640552828801792 80-81 0.0024734218141851377 82-83 0.002853948247136697 84-85 0.0017757900204406114 86-87 6.659212576652294E-4 88-89 0.0021246059173128744 90-91 0.007895923483744861 92-93 0.0022197375255507646 94-95 2.536842886343731E-4 96-97 0.0 98-99 5.390791133480428E-4 100-101 0.0020928953812335777 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1576763.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.60012519452529 #Duplication Level Percentage of deduplicated Percentage of total 1 80.34050303711993 51.09666051351967 2 12.27722647856683 15.616662821567829 3 3.5397849432216217 6.753922966517722 4 1.4041688210485177 3.5722125125173894 5 0.7242916495536793 2.303251979448163 6 0.42577740969763744 1.6247697937062255 7 0.2805220712292143 1.2488867195003899 8 0.1813890081256168 0.9229090900559997 9 0.13379347264575694 0.7658353449432393 >10 0.6389814860584344 7.147951741669877 >50 0.03391340861763664 1.470531663928545 >100 0.01587752379503589 1.9516035259348112 >500 0.0015876590821302663 0.6923120129969497 >1k 0.0017861164673965498 2.7653983680504406 >5k 2.9768607789942494E-4 1.2086745371848295 >10k+ 9.922869263314164E-5 0.8584164084580227 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13602 0.8626534235011857 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7611 0.48269778019905335 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6240 0.39574749026962197 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5301 0.33619510351270293 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4419 0.28025771786882364 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3990 0.2530500779127871 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3883 0.24626402319181767 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3507 0.2224177000601866 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3312 0.2100505909892609 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3132 0.19863479800071412 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3058 0.19394163866097822 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2779 0.17624715952873068 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2384 0.15119583602608636 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2331 0.14783451920168092 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1920 0.12176845854449908 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1664 0.10553266407189921 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 6.342107215859327E-5 0.0 0.0 0.0 0.0 6 6.342107215859327E-5 0.0 0.0 0.0 0.0 7 6.342107215859327E-5 0.0 0.0 0.0 0.0 8 6.342107215859327E-5 0.0 0.0 0.0 6.342107215859327E-5 9 6.342107215859327E-5 0.0 0.0 0.0 6.342107215859327E-5 10-11 6.342107215859327E-5 0.0 0.0 0.0 6.342107215859327E-5 12-13 6.342107215859327E-5 0.0 0.0 0.0 6.342107215859327E-5 14-15 9.513160823788991E-5 0.0 0.0 0.0 6.342107215859327E-5 16-17 1.2684214431718654E-4 0.0 0.0 0.0 6.342107215859327E-5 18-19 1.2684214431718654E-4 0.0 0.0 0.0 6.342107215859327E-5 20-21 1.2684214431718654E-4 0.0 0.0 3.1710536079296635E-5 6.342107215859327E-5 22-23 1.2684214431718654E-4 0.0 0.0 1.2684214431718654E-4 6.342107215859327E-5 24-25 1.5855268039648317E-4 0.0 0.0 4.122369690308563E-4 6.342107215859327E-5 26-27 1.902632164757798E-4 0.0 0.0 5.390791133480428E-4 6.342107215859327E-5 28-29 1.902632164757798E-4 0.0 0.0 9.513160823788991E-4 6.342107215859327E-5 30-31 1.902632164757798E-4 0.0 0.0 0.0018709216286785014 6.342107215859327E-5 32-33 2.2197375255507645E-4 0.0 0.0 0.0037735537934363 6.342107215859327E-5 34-35 3.1710536079296634E-4 0.0 0.0 0.0053273700613218344 6.342107215859327E-5 36-37 3.805264329515596E-4 0.0 0.0 0.00688118632920737 6.342107215859327E-5 38-39 3.805264329515596E-4 0.0 0.0 0.0094180292155511 6.342107215859327E-5 40-41 4.439475051101529E-4 0.0 0.0 0.012747635503877247 6.342107215859327E-5 42-43 4.439475051101529E-4 0.0 0.0 0.01848724253422994 6.342107215859327E-5 44-45 4.439475051101529E-4 0.0 0.0 0.029681061770221652 6.342107215859327E-5 46-47 4.7565804118944953E-4 0.0 0.0 0.041953039232909446 6.342107215859327E-5 48-49 5.073685772687462E-4 0.0 0.0 0.06430896716881357 6.342107215859327E-5 50-51 5.073685772687462E-4 0.0 0.0 0.08812357976436536 6.342107215859327E-5 52-53 5.073685772687462E-4 0.0 0.0 0.11320661380308898 9.513160823788991E-5 54-55 5.073685772687462E-4 0.0 0.0 0.16156518132401634 1.2684214431718654E-4 56-57 5.073685772687462E-4 0.0 0.0 0.2130630919167941 1.2684214431718654E-4 58-59 5.073685772687462E-4 0.0 0.0 0.2662099503856953 1.2684214431718654E-4 60-61 5.073685772687462E-4 0.0 0.0 0.32535010017358346 1.2684214431718654E-4 62-63 5.073685772687462E-4 0.0 0.0 0.4018676237329263 1.2684214431718654E-4 64-65 5.073685772687462E-4 0.0 0.0 0.45878803599526374 1.2684214431718654E-4 66-67 5.073685772687462E-4 0.0 0.0 0.5507803011613033 1.2684214431718654E-4 68-69 5.073685772687462E-4 0.0 0.0 0.6775907349424105 1.2684214431718654E-4 70-71 5.073685772687462E-4 0.0 0.0 0.7648264196965555 1.2684214431718654E-4 72-73 5.073685772687462E-4 0.0 0.0 0.894110275291848 2.536842886343731E-4 74-75 5.073685772687462E-4 0.0 0.0 1.050823744595732 2.536842886343731E-4 76-77 5.073685772687462E-4 0.0 0.0 1.1700553602538872 2.536842886343731E-4 78-79 5.073685772687462E-4 0.0 0.0 1.2915701345097519 2.536842886343731E-4 80-81 5.073685772687462E-4 0.0 0.0 1.4727641376668528 2.536842886343731E-4 82-83 5.390791133480428E-4 0.0 0.0 1.6706695933377431 2.536842886343731E-4 84-85 5.707896494273394E-4 0.0 0.0 1.8945776885936567 2.536842886343731E-4 86-87 5.707896494273394E-4 0.0 0.0 2.1944008072234062 2.536842886343731E-4 88-89 5.707896494273394E-4 0.0 0.0 2.5355110438284 2.536842886343731E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATAC 110 2.7325932E-6 30.229694 3 GTATAGA 545 0.0 22.664522 1 GAGTACT 5580 0.0 20.984106 12-13 CACGACC 185 6.5519E-6 20.541534 4 GTACTTT 5795 0.0 20.000969 14-15 TACATGG 4200 0.0 19.90699 2 GTACATG 4250 0.0 19.897606 1 GGACCGT 120 0.004766432 19.790115 6 AGTACTT 5870 0.0 19.259571 12-13 GTGTAGC 970 0.0 19.101286 1 GCGCTAC 225 1.6656395E-6 19.00333 1 TAAGGTG 775 0.0 19.00092 5 GTAAGGT 625 0.0 19.00092 4 AGGCGAC 305 4.5638444E-9 18.689428 5 AGAGTAC 8040 0.0 18.462685 10-11 TAGATCG 65 0.007562482 18.270695 74-75 ACATGGG 4280 0.0 18.202393 3 TATTACG 135 0.0093958555 17.594559 2 TACTTTT 6515 0.0 17.170822 14-15 TAGACCG 170 0.0017873828 16.765516 5 >>END_MODULE