FastQCFastQC Report
Thu 26 May 2016
SRR936462_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936462_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1734753
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT130600.7528449295086966No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT78580.4529751497763659No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT59140.34091308676220766No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC52060.3001003601089031No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT42630.24574103633197347No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT37870.21830197151986477No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34820.20072021780622373No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC34590.19939438064093276No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC33460.19288048500276408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28530.16446145359022293No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG27850.16054158718849312No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC27340.15760168738719577No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA25980.14976195458373615No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA20730.11949828015861624No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG1401.7751272E-523.7586881
GAGTACT44050.021.35793312-13
TAGCGCT1604.977123E-520.7888534
GTACATG50350.020.1960641
TACATGG48800.019.9606172
GTACTTT46950.019.6313314-15
GTATAGC7800.019.4943081
AGTACTT48100.018.86810112-13
ACATGGG49150.018.465023
GTATAGG5150.018.453351
AGAGTAC72150.018.17738710-11
GTATAGT5350.017.7635041
GTATTAC3501.3060344E-917.6493111
CATGGGG29600.016.5347634
GTCCTAC4409.640644E-1116.1991061
GTGTAGC12050.016.1677381
TACTTTT56050.016.14741314-15
ACTTTTT60300.015.63896416-17
GAGTACA45500.014.9340331
TAGCCCT13100.014.871854
Produced by FastQC (version 0.11.5)