##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936462_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1734753 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.441174046103395 31.0 31.0 34.0 30.0 34.0 2 31.500788296662407 31.0 31.0 34.0 28.0 34.0 3 31.558594797069095 33.0 31.0 34.0 28.0 34.0 4 35.18225361189749 37.0 35.0 37.0 33.0 37.0 5 35.10229640761538 37.0 35.0 37.0 32.0 37.0 6 35.22413219634149 37.0 35.0 37.0 32.0 37.0 7 35.05231119358203 37.0 35.0 37.0 32.0 37.0 8 35.050815303389015 37.0 35.0 37.0 32.0 37.0 9 36.57937412415485 39.0 37.0 39.0 32.0 39.0 10-11 36.45739508736979 39.0 37.0 39.0 32.0 39.0 12-13 36.37506953439481 39.0 37.0 39.0 32.0 39.0 14-15 37.47262348011503 40.0 37.0 41.0 32.0 41.0 16-17 37.42002117880759 40.0 37.0 41.0 32.0 41.0 18-19 37.38448182536649 40.0 37.0 41.0 32.0 41.0 20-21 37.27728846700366 39.5 37.0 41.0 31.0 41.0 22-23 37.18100473093288 39.0 36.0 41.0 31.0 41.0 24-25 37.03078420962523 39.0 36.0 41.0 31.0 41.0 26-27 36.82603575264029 39.0 36.0 40.5 30.5 41.0 28-29 36.96659466794408 39.0 36.0 41.0 30.5 41.0 30-31 36.946963342908184 39.0 36.0 41.0 30.0 41.0 32-33 36.82148323133033 39.0 36.0 41.0 30.0 41.0 34-35 36.649311458173 39.0 36.0 41.0 30.0 41.0 36-37 36.41821861671373 39.0 35.0 40.5 30.0 41.0 38-39 36.25620794430101 39.0 35.0 40.0 30.0 41.0 40-41 36.16067186510126 39.0 35.0 40.0 30.0 41.0 42-43 35.91908732828247 38.0 35.0 40.0 29.0 41.0 44-45 35.68529006723148 38.0 35.0 40.0 27.5 41.0 46-47 35.40048295059873 38.0 34.5 40.0 27.0 41.0 48-49 35.18468882889956 38.0 34.0 40.0 26.0 41.0 50-51 34.504010369199534 37.5 33.5 39.5 25.0 40.5 52-53 34.71875304438153 38.0 33.5 39.5 26.0 40.5 54-55 35.09819524739257 38.0 34.0 40.0 26.0 41.0 56-57 35.00830665806602 38.0 34.0 40.0 26.0 41.0 58-59 34.78868893727234 38.0 34.0 40.0 26.0 41.0 60-61 34.44497919876778 38.0 34.0 40.0 25.5 41.0 62-63 34.15677505673719 37.0 33.0 40.0 25.0 41.0 64-65 33.86237579643903 37.0 33.0 40.0 24.0 41.0 66-67 33.466533852369764 36.0 33.0 39.0 23.0 41.0 68-69 33.07894207417425 36.0 32.0 39.0 22.0 41.0 70-71 32.65861191766206 35.0 32.0 38.5 21.5 40.0 72-73 32.23711877137552 35.0 31.5 38.0 20.5 40.0 74-75 31.83452370452739 35.0 31.0 37.0 20.0 39.0 76-77 31.40961162770723 35.0 31.0 36.5 18.5 39.0 78-79 30.94403208987101 34.5 30.5 36.0 17.5 39.0 80-81 30.50125637482685 34.0 30.0 36.0 14.0 37.5 82-83 30.08756520380711 34.0 30.0 35.0 9.5 37.0 84-85 29.698054420427574 34.0 29.0 35.0 7.0 36.5 86-87 29.40465904944393 34.0 29.0 35.0 6.0 36.0 88-89 29.008750813516393 34.0 29.0 35.0 2.0 36.0 90-91 28.6414613492526 34.0 29.0 35.0 2.0 35.5 92-93 28.279103422792755 34.0 29.0 35.0 2.0 35.0 94-95 27.840438667637407 34.0 27.0 35.0 2.0 35.0 96-97 27.264467909840768 33.0 26.5 35.0 2.0 35.0 98-99 26.622845730775506 33.0 25.0 35.0 2.0 35.0 100-101 24.49999654129435 31.0 20.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29923069210127196 1101 2 0.32362734752001643 1101 3 0.333753240273154 1101 4 0.2802717694518009 1101 5 0.39197575253310646 1101 6 0.27432837882175676 1101 7 0.3372855221699993 1101 8 0.191079657431807 1101 9 0.49741682250770936 1101 10-11 0.44171776034286836 1101 12-13 0.5073451635727366 1101 14-15 0.7231589099594444 1101 16-17 0.5902703890866121 1101 18-19 0.6013543862470669 1101 20-21 0.5965794941967673 1101 22-23 0.4868238557366382 1101 24-25 0.5197803975755804 1101 26-27 0.6515170441065479 1101 28-29 0.6884411090920892 1101 30-31 0.5652769872594732 1101 32-33 0.49687213319388235 1101 34-35 0.6007959970789685 1101 36-37 0.6144244705331303 1101 38-39 0.5653902331787464 1101 40-41 0.46250057294181346 1101 42-43 0.3480310638945454 1101 44-45 0.5381187474669389 1101 46-47 0.6144595523635275 1101 48-49 0.5664865697444696 1101 50-51 0.7609007834087791 1101 52-53 0.6422769440496126 1101 54-55 0.6343234037490504 1101 56-57 0.6777042948978149 1101 58-59 0.7222895356290593 1101 60-61 0.7850934458779406 1101 62-63 0.839603780270096 1101 64-65 0.8248078268150181 1101 66-67 0.5056420822111747 1101 68-69 0.6000442750864394 1101 70-71 0.5769894966027849 1101 72-73 0.6212366902301198 1101 74-75 0.6011052863981199 1101 76-77 0.55515722870161 1101 78-79 0.5275653807445728 1101 80-81 0.5370301582248942 1101 82-83 0.5877670370331138 1101 84-85 0.6282718974497321 1101 86-87 0.6266822408870212 1101 88-89 0.6064698523875869 1101 90-91 0.660957310122587 1101 92-93 0.34668348860346754 1101 94-95 0.20373975023962743 1101 96-97 -0.015664685307388737 1101 98-99 -0.02760232487801062 1101 100-101 0.3850472101381186 1102 1 0.1829173061533531 1102 2 0.22053808882243686 1102 3 0.306130132623494 1102 4 0.2804222532267744 1102 5 0.30189445530447756 1102 6 0.1990702957736019 1102 7 0.16103932981439328 1102 8 0.22781186964227373 1102 9 0.2960045244067686 1102 10-11 0.4084696186712833 1102 12-13 0.4450332964087451 1102 14-15 0.3880300810021424 1102 16-17 0.2772777459212037 1102 18-19 0.1835916323534974 1102 20-21 0.09755741379569827 1102 22-23 -0.13664931878719955 1102 24-25 0.032605146735548374 1102 26-27 0.04945564132783886 1102 28-29 0.1408063586319841 1102 30-31 0.20706689391454347 1102 32-33 0.168580193770687 1102 34-35 0.03433705350656169 1102 36-37 -0.12825108599815138 1102 38-39 0.049678062527902966 1102 40-41 -0.09099584671402283 1102 42-43 -0.031451001993502814 1102 44-45 0.024156597397954727 1102 46-47 0.12406329641784453 1102 48-49 0.250171699203257 1102 50-51 0.38493922807685976 1102 52-53 0.27258824526865055 1102 54-55 0.18057410836851773 1102 56-57 0.25062324375178235 1102 58-59 0.2625919697772261 1102 60-61 -0.1166590940137553 1102 62-63 0.0881360573527914 1102 64-65 0.3036624945787807 1102 66-67 0.22721065849969335 1102 68-69 0.24493653185270148 1102 70-71 0.3392640464898449 1102 72-73 0.10641212641937159 1102 74-75 0.23554904303098212 1102 76-77 0.18147013778016685 1102 78-79 -0.03802282636609178 1102 80-81 0.01605589162522847 1102 82-83 0.012204162482596814 1102 84-85 0.08103528791891534 1102 86-87 0.0680081896433613 1102 88-89 -0.2540441722953517 1102 90-91 -0.41868875833695895 1102 92-93 -0.32949668433815305 1102 94-95 -0.06301062349549014 1102 96-97 -0.261195272203107 1102 98-99 -0.3804900387887855 1102 100-101 -0.002602143321496442 1103 1 0.18370796985263382 1103 2 0.3338118947630164 1103 3 0.3754223718100427 1103 4 0.3291262386210576 1103 5 0.42146212398763794 1103 6 0.14876566562901417 1103 7 0.3310760507523369 1103 8 0.08235041404837062 1103 9 0.4992050951222069 1103 10-11 0.34361318253399986 1103 12-13 0.4469728932960493 1103 14-15 0.553480727863878 1103 16-17 0.6072461912517539 1103 18-19 0.40467108596893553 1103 20-21 0.3404160584004501 1103 22-23 0.3586797006370759 1103 24-25 0.3291309884105331 1103 26-27 0.3695475290284378 1103 28-29 0.5011472964130377 1103 30-31 0.2321024063949011 1103 32-33 0.22972230748430178 1103 34-35 0.148522593525378 1103 36-37 0.38466368674443174 1103 38-39 0.20774004696799864 1103 40-41 0.24887027056117716 1103 42-43 0.2234958123954982 1103 44-45 0.330921533978632 1103 46-47 0.2701988377394571 1103 48-49 0.3778591696095219 1103 50-51 -0.10255233742828551 1103 52-53 0.1462561341696187 1103 54-55 0.31710016567905086 1103 56-57 0.40033900913670806 1103 58-59 0.39099463143875113 1103 60-61 0.3894004896838368 1103 62-63 0.40534898578244594 1103 64-65 0.4664102845389735 1103 66-67 0.32825882699012965 1103 68-69 0.22630764154022387 1103 70-71 0.2863774052022805 1103 72-73 0.17069151282840522 1103 74-75 0.014962631125744252 1103 76-77 0.10278719482205645 1103 78-79 0.3666393041670979 1103 80-81 0.3484616925810329 1103 82-83 0.27411622989745865 1103 84-85 0.2133400546326918 1103 86-87 0.12332275250067326 1103 88-89 0.04263912825838645 1103 90-91 0.24791014785169097 1103 92-93 0.29983590565150564 1103 94-95 0.29813467982736697 1103 96-97 0.3124033887001012 1103 98-99 0.15409004241596946 1103 100-101 0.14620840197589402 1104 1 0.23925926342532122 1104 2 0.19707074081289022 1104 3 0.23262204814288978 1104 4 0.2179647447838846 1104 5 0.1944055954822801 1104 6 0.11903513862873893 1104 7 0.3089759952247917 1104 8 0.3249281708815488 1104 9 0.3810232022173068 1104 10-11 0.4153587933004985 1104 12-13 0.3231635847601453 1104 14-15 0.339299192965548 1104 16-17 0.18454592676526715 1104 18-19 0.410690299596574 1104 20-21 0.29535769054505323 1104 22-23 0.3638755905025661 1104 24-25 0.3662262218088941 1104 26-27 0.24965368893316509 1104 28-29 0.1913579175178839 1104 30-31 0.17643740920781426 1104 32-33 0.1789709960112802 1104 34-35 0.2189046742010632 1104 36-37 0.3578645667830145 1104 38-39 0.25028255111429587 1104 40-41 0.07002521616609414 1104 42-43 0.2218213097688988 1104 44-45 0.2344990423991007 1104 46-47 0.33485167183629017 1104 48-49 0.2717036029728135 1104 50-51 0.08803478419897459 1104 52-53 0.1472977323401281 1104 54-55 0.3045630864280895 1104 56-57 0.3918196859152303 1104 58-59 0.2865544368000812 1104 60-61 0.09304320644219644 1104 62-63 -0.036708063293673376 1104 64-65 -0.03104211520092548 1104 66-67 -0.044842459641614596 1104 68-69 0.13586509246044187 1104 70-71 0.08205041187289197 1104 72-73 0.0039284949061624275 1104 74-75 -0.22581504679986253 1104 76-77 -0.05714789822311772 1104 78-79 0.014836439749295494 1104 80-81 0.17758662225831756 1104 82-83 0.09982868121952748 1104 84-85 0.05780926424187882 1104 86-87 -0.1938788234588209 1104 88-89 -0.17612320104920087 1104 90-91 -0.28231880282377375 1104 92-93 -0.3776250698067827 1104 94-95 -0.27685331051376494 1104 96-97 -0.04672491098792264 1104 98-99 -0.010534525601620714 1104 100-101 -0.22293449195917958 1105 1 0.14356030092034544 1105 2 0.24552328068501694 1105 3 0.17932015582690752 1105 4 0.31702883293583284 1105 5 0.43245566691252435 1105 6 0.3146208080823598 1105 7 0.40987267898787394 1105 8 0.11508426353008616 1105 9 0.31519174246415105 1105 10-11 0.41614920993788473 1105 12-13 0.2524147089082476 1105 14-15 0.3964711993357213 1105 16-17 0.44021789800581956 1105 18-19 0.31554389687050843 1105 20-21 -0.0248569222093451 1105 22-23 -0.014835708356891075 1105 24-25 0.006977468910697837 1105 26-27 -0.018988433833179386 1105 28-29 0.22136886625204966 1105 30-31 0.07854138119778753 1105 32-33 -0.06151546151699705 1105 34-35 -0.09174955657717732 1105 36-37 -0.13667204414464607 1105 38-39 0.03487352514179065 1105 40-41 -0.07735748014997768 1105 42-43 0.05516196177480026 1105 44-45 0.16743072698186978 1105 46-47 0.16377550494713944 1105 48-49 0.31951722272971494 1105 50-51 0.2332775006560368 1105 52-53 0.41509820776110473 1105 54-55 0.44686881958343605 1105 56-57 0.5297847143702299 1105 58-59 0.4523223704753434 1105 60-61 0.5450532066763927 1105 62-63 0.4080820664517475 1105 64-65 0.4393772740119388 1105 66-67 0.00332026079680503 1105 68-69 0.4040380883151471 1105 70-71 0.4715494340982431 1105 72-73 0.41144535712776076 1105 74-75 0.29768081961386983 1105 76-77 0.6200422877297171 1105 78-79 0.5020573055775337 1105 80-81 0.6449394876650025 1105 82-83 0.4944324775613609 1105 84-85 0.28773048605035356 1105 86-87 0.49159244050520456 1105 88-89 0.5477571884212793 1105 90-91 0.5273803411842835 1105 92-93 0.42709737685473215 1105 94-95 0.530577044998072 1105 96-97 0.6901358761499488 1105 98-99 0.3034131078324158 1105 100-101 0.44639690338965465 1106 1 -0.011209932821110158 1106 2 0.03960236055993249 1106 3 0.04333298245541428 1106 4 0.14035050569779628 1106 5 0.19796740698778592 1106 6 0.15928503662955507 1106 7 0.38827730081434453 1106 8 0.21782290772365087 1106 9 0.40951463428585555 1106 10-11 0.3943428814277894 1106 12-13 0.24499090014162306 1106 14-15 0.30061600243107023 1106 16-17 0.22176848898477886 1106 18-19 0.2415771825015085 1106 20-21 0.19460748654668691 1106 22-23 0.17513885039648613 1106 24-25 0.048589542902320204 1106 26-27 0.06376425604862135 1106 28-29 -0.12711923466489594 1106 30-31 -0.0669642635788037 1106 32-33 -0.028332655559502484 1106 34-35 -0.10138092351416361 1106 36-37 0.049594982939680676 1106 38-39 -0.1537362167355596 1106 40-41 -0.2192806793867419 1106 42-43 -0.25735963678352647 1106 44-45 -0.3239383301019174 1106 46-47 -0.2391982057646871 1106 48-49 -0.4471891958847962 1106 50-51 -0.37353651323065407 1106 52-53 -0.35740749001809036 1106 54-55 -0.28710664021409116 1106 56-57 -0.21358678083303317 1106 58-59 -0.1960955020004107 1106 60-61 -0.19005756875159818 1106 62-63 -0.25741032767555794 1106 64-65 -0.2425976027570229 1106 66-67 -0.18160500554124326 1106 68-69 -0.26650995853915305 1106 70-71 -0.2361385926150703 1106 72-73 -0.08236791472256755 1106 74-75 -0.24757147274845082 1106 76-77 -0.3049309459587377 1106 78-79 -0.064584714881466 1106 80-81 -0.1575031370236104 1106 82-83 0.0063943686940284294 1106 84-85 0.024551920300634578 1106 86-87 0.05505324508463261 1106 88-89 0.22318677447099233 1106 90-91 0.20173821472008768 1106 92-93 0.20351069110713382 1106 94-95 0.15092871723001267 1106 96-97 0.18580211879583786 1106 98-99 0.32358828141592255 1106 100-101 0.6279758507580766 1107 1 0.2394918215648545 1107 2 0.17120080110574065 1107 3 0.32845968810843473 1107 4 0.288518147023332 1107 5 0.22792842063301322 1107 6 0.3063025804892092 1107 7 0.4680823690404665 1107 8 0.491409652363032 1107 9 0.6089883184963796 1107 10-11 0.48870935316269026 1107 12-13 0.49476672860166104 1107 14-15 0.5456062558423724 1107 16-17 0.6070803798229818 1107 18-19 0.7318111006301606 1107 20-21 0.742901833525238 1107 22-23 0.7054507412346922 1107 24-25 0.5611723889062219 1107 26-27 0.6935985640408262 1107 28-29 0.6466916815144472 1107 30-31 0.4809012334972138 1107 32-33 0.6884509701714876 1107 34-35 0.544223364984866 1107 36-37 0.3280745151034239 1107 38-39 0.5771214830695044 1107 40-41 0.540222244589863 1107 42-43 0.6400965035673494 1107 44-45 0.7369193697031449 1107 46-47 0.5007140749370649 1107 48-49 0.8062610965128627 1107 50-51 0.7102451631826057 1107 52-53 0.6713352000403887 1107 54-55 0.5539752311309343 1107 56-57 0.5998440183872376 1107 58-59 0.5400643420541726 1107 60-61 0.4934609497669129 1107 62-63 0.5800458126753156 1107 64-65 0.32976107170691193 1107 66-67 0.30099733364004067 1107 68-69 0.35477659116845217 1107 70-71 0.5299627719073499 1107 72-73 0.7189478747511231 1107 74-75 0.6524967533724002 1107 76-77 0.6950495608638789 1107 78-79 0.6695575853134663 1107 80-81 0.7021947187268793 1107 82-83 0.6843183453022128 1107 84-85 0.8498150781953697 1107 86-87 0.9104041222776154 1107 88-89 0.8602043183306449 1107 90-91 0.9715237897688205 1107 92-93 0.8931598139141457 1107 94-95 0.9620495749298179 1107 96-97 0.8267085515443746 1107 98-99 0.9705765855094874 1107 100-101 1.4638043107970624 1108 1 0.013425285361332584 1108 2 0.07739925004391424 1108 3 -0.22098294434789523 1108 4 0.019967012718772992 1108 5 0.1977885351506572 1108 6 0.10020995165685065 1108 7 0.23996611188311334 1108 8 0.058864896256629606 1108 9 -0.007410898724756976 1108 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88-89 -2.9255298840955746 2316 90-91 -3.1166762625376947 2316 92-93 -3.3640464145848554 2316 94-95 -3.472809096887026 2316 96-97 -3.6754702160336272 2316 98-99 -4.3542788674448225 2316 100-101 -5.291517273688022 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3269.0 3 1288.0 4 1215.0 5 1424.0 6 1551.0 7 1868.0 8 2247.0 9 2911.0 10 3761.0 11 4633.0 12 5876.0 13 7013.0 14 8047.0 15 9189.0 16 10977.0 17 12593.0 18 12010.0 19 9535.0 20 9837.0 21 11183.0 22 12273.0 23 14249.0 24 16126.0 25 19233.0 26 22619.0 27 26785.0 28 31983.0 29 39236.0 30 47838.0 31 60010.0 32 74820.0 33 95942.0 34 127308.0 35 172402.0 36 239904.0 37 316249.0 38 256983.0 39 40356.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.82969547873643 18.620945717278765 14.688615059270923 22.860743744713872 2 15.171061627843965 20.285673437339664 40.094013851881485 24.449251082934882 3 20.60616926597069 23.47997931548554 29.582775818027002 26.33107560051677 4 12.680221960683458 16.35861539251093 38.281644188236406 32.6795184585692 5 13.80067625043758 37.29683218827514 34.56316793860112 14.339323622686168 6 31.668357656086783 36.285422094608116 17.424939392593526 14.621280856711575 7 28.13847447209188 32.38757122478375 20.949917488228667 18.524036814895712 8 25.548371175297724 34.15607748982597 19.879860556137917 20.415690778738384 9 25.026246530009832 18.01996523523867 21.205639616838138 35.748148617913365 10-11 25.016868842872352 26.07522292824657 27.5246169763421 21.383291252538978 12-13 26.438723157257034 23.544480529685067 27.42946053125945 22.587335781798448 14-15 22.789832824513308 25.470982844260714 26.573320533554735 25.165863797671246 16-17 22.02697883570275 28.536384757600718 27.078786145548257 22.35785026114828 18-19 21.74712540284147 28.18007626382231 28.436816524558555 21.635981808777665 20-21 22.912347119751104 26.510524895581323 28.16144707800669 22.415680906660885 22-23 22.172602060524298 26.51720082328727 28.417391655280166 22.89280546090827 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27.10370559319323 27.647100362157495 22.34593895059353 50-51 22.019856144552964 27.457107816645554 28.32810916988403 22.194926868917452 52-53 22.76144214243242 27.82505835893998 26.99519264719149 22.41830685143611 54-55 22.248803827751196 27.088343805845387 27.585230875655732 23.07762149074768 56-57 22.91075249198785 27.01276496151085 27.240238207031208 22.836244339470095 58-59 23.605849270657032 26.862643482030446 27.149117548042558 22.382389699269964 60-61 22.729713487736653 27.210983762719827 28.178648992573823 21.880653756969696 62-63 22.268457176686862 27.342161368990197 27.9737839449577 22.415597509365245 64-65 22.085249972833896 28.06369521850627 27.386834080965656 22.46422072769418 66-67 22.480241654224624 27.27801765136536 26.97684339169083 23.264897302719188 68-69 22.32251128107791 27.286646749132753 27.38490850472933 23.005933465060004 70-71 23.105825638991632 27.07724165921061 27.014806803612114 22.80212589818564 72-73 22.67085838704852 26.726656269032585 27.981981789824633 22.620503554094263 74-75 22.11383979141074 28.210827110839276 27.192870702213234 22.482462395536754 76-77 22.415739680411324 27.222165696229055 27.03455384321829 23.327540780141334 78-79 22.618449004823074 27.331469226670603 27.292343746035552 22.757738022470768 80-81 23.111730350605445 27.200959648244293 26.939627897027506 22.747682104122756 82-83 22.814878312270924 27.094788336332915 27.65065262531663 22.439680726079533 84-85 22.543677672883913 27.59377393368713 27.37381100829264 22.488737385136318 86-87 22.500950482937572 27.741592009198428 27.065805126064735 22.69165238179927 88-89 22.30823309060628 27.724294813204516 26.567759932828512 23.399712163360693 90-91 22.554665073908183 27.060415089618605 26.98944432369357 23.395475512779637 92-93 23.16253337749555 27.41870996177893 27.163768305194335 22.25498835553119 94-95 22.643045841254104 27.255304092665426 27.33148759604007 22.770162470040397 96-97 21.75433307084526 27.37939864482366 28.104228901896715 22.76203938243436 98-99 22.323937205908553 27.811962520043338 26.779741542591616 23.084358731456494 100-101 22.120039168782156 27.208664882003468 27.162097167644927 23.509198781569452 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 99.0 1 69.5 2 72.5 3 103.0 4 86.0 5 96.5 6 158.5 7 277.5 8 470.5 9 618.5 10 637.0 11 609.5 12 698.5 13 828.0 14 904.5 15 993.0 16 1119.0 17 1247.5 18 1412.5 19 1657.0 20 1877.0 21 2238.0 22 2762.5 23 3398.0 24 4390.0 25 5804.0 26 7720.0 27 10149.5 28 12686.0 29 15644.5 30 19046.0 31 22856.0 32 27162.0 33 30652.0 34 33677.5 35 36783.0 36 40692.5 37 44535.0 38 47336.0 39 49609.5 40 51822.5 41 54624.5 42 56537.5 43 58191.5 44 60585.5 45 63328.0 46 65953.0 47 76622.0 48 97100.5 49 94286.0 50 72350.0 51 61763.5 52 58516.5 53 56431.5 54 53695.5 55 49255.5 56 44330.5 57 39619.5 58 35794.5 59 31748.5 60 26402.0 61 21807.0 62 17734.0 63 13416.0 64 9524.5 65 7097.5 66 5465.0 67 4299.0 68 3392.5 69 2778.0 70 2193.5 71 1589.5 72 1245.5 73 1039.0 74 822.5 75 648.5 76 494.0 77 347.5 78 240.0 79 154.5 80 105.5 81 79.5 82 56.5 83 42.0 84 26.5 85 17.5 86 15.5 87 9.0 88 5.5 89 5.5 90 5.0 91 3.0 92 1.5 93 1.5 94 2.0 95 1.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014987724477202228 2 0.006629185826454832 3 0.007032701485456431 4 0.03297299384984491 5 0.04496317343160669 6 0.06075792922681212 7 0.026228517835103903 8 0.025075615952242194 9 0.01371953240605435 10-11 0.045366689090608286 12-13 0.042743837307097896 14-15 0.020060492761793756 16-17 0.022683344545304143 18-19 0.029398998012973604 20-21 0.02242394162166026 22-23 0.014007757876769776 24-25 0.0031704801778697023 26-27 0.0024499165010811335 28-29 0.001383482259434052 30-31 0.006975056391313345 32-33 0.001268192071147881 34-35 0.0017005302772210222 36-37 0.005533929037736208 38-39 0.006658008373526375 40-41 0.003660463478085929 42-43 8.07031318003197E-4 44-45 0.0012105469770047955 46-47 0.0024499165010811335 48-49 6.052734885023977E-4 50-51 0.0014699499006486803 52-53 8.358538650747397E-4 54-55 0.0030551899895835313 56-57 0.0025075615952242195 58-59 0.0023634488598665055 60-61 6.052734885023977E-4 62-63 8.358538650747397E-4 64-65 0.0031416576307981593 66-67 0.0024787390481526765 68-69 0.005793331961380093 70-71 0.008358538650747397 72-73 0.003660463478085929 74-75 0.0029975448954404457 76-77 0.0031416576307981593 78-79 0.03395296045027736 80-81 0.02118457209758392 82-83 0.0038622213075867285 84-85 0.008214425915389683 86-87 0.006254492714524777 88-89 0.005101590831663067 90-91 0.013604242217768178 92-93 0.013748354953125892 94-95 0.00723445931495723 96-97 0.00409280168415907 98-99 0.022251006339231 100-101 0.041446822688878476 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1734753.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.79263876981722 #Duplication Level Percentage of deduplicated Percentage of total 1 81.06374432360025 52.52333903287851 2 11.82169955896511 15.319182183386676 3 3.3547185051004416 6.520831928261823 4 1.39543851905798 3.6165657556324957 5 0.696241458778106 2.2555660667590196 6 0.4123791687341725 1.6031480709594443 7 0.2697698717478157 1.2235371295795299 8 0.17832279509117546 0.9243203557413333 9 0.13532426243696302 0.7891214447583079 >10 0.6205214929006462 6.984362260620664 >50 0.03437658518381735 1.5168014041588693 >100 0.013989054292388203 1.717514571440738 >500 0.0013363092735270326 0.5311578688110082 >1k 0.0017817456980360432 2.6252544046287185 >5k 2.6726185470540647E-4 1.0954481671696712 >10k+ 8.908728490180215E-5 0.7538493552131893 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13060 0.7528449295086966 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7858 0.4529751497763659 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5914 0.34091308676220766 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5206 0.3001003601089031 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4263 0.24574103633197347 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3787 0.21830197151986477 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3482 0.20072021780622373 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3459 0.19939438064093276 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3346 0.19288048500276408 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2853 0.16446145359022293 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2785 0.16054158718849312 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2734 0.15760168738719577 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2598 0.14976195458373615 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2073 0.11949828015861624 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 5.76450941430855E-5 0.0 0.0 0.0 0.0 6 5.76450941430855E-5 0.0 0.0 0.0 0.0 7 5.76450941430855E-5 0.0 0.0 0.0 0.0 8 5.76450941430855E-5 0.0 0.0 0.0 0.0 9 5.76450941430855E-5 0.0 0.0 0.0 0.0 10-11 1.15290188286171E-4 0.0 0.0 0.0 0.0 12-13 1.15290188286171E-4 0.0 0.0 2.882254707154275E-5 0.0 14-15 1.15290188286171E-4 0.0 0.0 5.76450941430855E-5 0.0 16-17 1.4411273535771375E-4 0.0 0.0 5.76450941430855E-5 0.0 18-19 1.729352824292565E-4 0.0 0.0 5.76450941430855E-5 5.76450941430855E-5 20-21 1.729352824292565E-4 0.0 0.0 5.76450941430855E-5 5.76450941430855E-5 22-23 1.729352824292565E-4 0.0 0.0 8.646764121462825E-5 5.76450941430855E-5 24-25 2.0175782950079927E-4 0.0 0.0 1.729352824292565E-4 5.76450941430855E-5 26-27 2.30580376572342E-4 0.0 0.0 2.5940292364388473E-4 5.76450941430855E-5 28-29 2.30580376572342E-4 0.0 0.0 6.052734885023977E-4 5.76450941430855E-5 30-31 2.30580376572342E-4 0.0 0.0 0.001498772447720223 5.76450941430855E-5 32-33 2.882254707154275E-4 0.0 0.0 0.002738141971796561 1.729352824292565E-4 34-35 3.45870564858513E-4 0.0 0.0 0.0038333987605151856 1.729352824292565E-4 36-37 4.035156590015985E-4 0.0 0.0 0.005159235925806152 1.729352824292565E-4 38-39 4.035156590015985E-4 0.0 0.0 0.007839732803459627 2.0175782950079927E-4 40-41 4.61160753144684E-4 0.0 0.0 0.01080845515182853 2.30580376572342E-4 42-43 4.61160753144684E-4 0.0 0.0 0.01709177041342485 2.30580376572342E-4 44-45 4.61160753144684E-4 0.0 0.0 0.026776146229463213 2.30580376572342E-4 46-47 4.61160753144684E-4 0.0 0.0 0.03692168279864626 2.30580376572342E-4 48-49 5.188058472877695E-4 0.0 0.0 0.05620396678950836 2.882254707154275E-4 50-51 5.188058472877695E-4 0.0 0.0 0.07499626748015423 2.882254707154275E-4 52-53 5.188058472877695E-4 0.0 0.0 0.09390385835908628 2.882254707154275E-4 54-55 5.188058472877695E-4 0.0 0.0 0.13062378332823174 2.882254707154275E-4 56-57 5.188058472877695E-4 0.0 0.0 0.17186884818760942 2.882254707154275E-4 58-59 5.188058472877695E-4 0.0 0.0 0.2155350070009967 3.45870564858513E-4 60-61 5.188058472877695E-4 0.0 0.0 0.2675885270122029 3.45870564858513E-4 62-63 5.188058472877695E-4 0.0 0.0 0.33174751679345704 3.45870564858513E-4 64-65 5.188058472877695E-4 0.0 0.0 0.38282107020423084 3.45870564858513E-4 66-67 5.188058472877695E-4 0.0 0.0 0.46461945879326916 3.45870564858513E-4 68-69 6.340960355739405E-4 0.0 0.0 0.578468519725863 3.45870564858513E-4 70-71 6.91741129717026E-4 0.0 0.0 0.6532053842823733 3.45870564858513E-4 72-73 6.91741129717026E-4 0.0 0.0 0.7617222740067318 3.45870564858513E-4 74-75 6.91741129717026E-4 0.0 0.0 0.8915390260169603 3.45870564858513E-4 76-77 6.91741129717026E-4 2.882254707154275E-5 0.0 0.9980959825404538 3.45870564858513E-4 78-79 6.91741129717026E-4 5.76450941430855E-5 0.0 1.1044511812344466 3.45870564858513E-4 80-81 6.91741129717026E-4 5.76450941430855E-5 0.0 1.257037745431194 3.45870564858513E-4 82-83 6.91741129717026E-4 5.76450941430855E-5 0.0 1.429425399466091 3.45870564858513E-4 84-85 6.91741129717026E-4 5.76450941430855E-5 0.0 1.6415593459126456 3.45870564858513E-4 86-87 6.91741129717026E-4 5.76450941430855E-5 0.0 1.9203598437356788 3.45870564858513E-4 88-89 6.91741129717026E-4 5.76450941430855E-5 0.0 2.2448152561200354 3.45870564858513E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTACG 140 1.7751272E-5 23.758688 1 GAGTACT 4405 0.0 21.357933 12-13 TAGCGCT 160 4.977123E-5 20.788853 4 GTACATG 5035 0.0 20.196064 1 TACATGG 4880 0.0 19.960617 2 GTACTTT 4695 0.0 19.63133 14-15 GTATAGC 780 0.0 19.494308 1 AGTACTT 4810 0.0 18.868101 12-13 ACATGGG 4915 0.0 18.46502 3 GTATAGG 515 0.0 18.45335 1 AGAGTAC 7215 0.0 18.177387 10-11 GTATAGT 535 0.0 17.763504 1 GTATTAC 350 1.3060344E-9 17.649311 1 CATGGGG 2960 0.0 16.534763 4 GTCCTAC 440 9.640644E-11 16.199106 1 GTGTAGC 1205 0.0 16.167738 1 TACTTTT 5605 0.0 16.147413 14-15 ACTTTTT 6030 0.0 15.638964 16-17 GAGTACA 4550 0.0 14.934033 1 TAGCCCT 1310 0.0 14.87185 4 >>END_MODULE