FastQCFastQC Report
Thu 26 May 2016
SRR936462_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936462_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1734753
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT143190.8254201030348413No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT76180.43914032718202534No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT62580.360742999147429No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC53780.31001531630151385No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT45420.2618240175978943No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT40850.23548020957450425No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40530.23363556656192555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36530.21057752890469134No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC36270.2090787564569711No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC33410.19259225953204864No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA31900.18388785031644275No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG27940.1610603930357809No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC25050.14440096082842918No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA23780.1370800338722573No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20960.1208241173239072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT55250.022.26102312-13
GTACTTT57750.021.33845714-15
GTACATG49550.021.1250951
TACATGG48350.021.0242312
ACATGGG48950.019.9896343
AGTACTT57900.019.9299112-13
AGAGTAC81000.019.52256410-11
GTATAGC5500.019.2063941
AGCGTAA757.7317416E-418.99766592-93
GCGTATC1300.007107589418.4676861
CATGGGG29000.017.5257284
TACTTTT66700.017.4072714-15
ACTTTTT73150.017.00844416-17
GTATAGA6550.016.1275061
ATAGCCC6900.015.8332123
GTCTAGA8100.015.4125391
GCTATAC6250.015.1998843
GAGTACA43700.014.943191
TCTAGAC10000.014.7248883
CGATTCG1950.00446802714.6127459
Produced by FastQC (version 0.11.5)