FastQCFastQC Report
Thu 26 May 2016
SRR936461_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936461_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1750167
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT133580.763241450672993No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT80870.46207019101605734No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT61380.3507093894468356No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC53030.3029996566041983No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT41800.23883435123619629No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT36530.20872293901096295No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC36190.2067802672544963No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33560.19175312984418058No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC33520.19152458022577273No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG28300.1616988550235492No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27610.15775637410601387No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC27170.1552423283035276No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA26330.1504427863169629No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA21850.12484522905528443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG52300.023.4473461
TACATGG51500.023.3501112
GGACCGT2103.4293407E-822.6311536
ACATGGG52750.021.5353073
GAGTACT44350.020.52288212-13
CTAGGCG1200.00474503819.8056584
ACGACGG1200.004749750719.802266
GTACTTT46900.019.35525714-15
GTACTAG3254.838512E-1019.0123441
AGAGTAC72150.019.00521310-11
CATGGGG33250.018.2986414
TATACCG1300.00754136118.2790095
AGTACTT47700.018.03530112-13
GTATTAG5750.017.3590951
GTGTAGC12800.015.9673971
ACTTTTT59650.015.9327116-17
TAGCCCT12600.015.8445254
TACTTTT55700.015.61472314-15
GACCGTG4702.582965E-1015.1694237
TATAGGG4401.6516424E-915.1234542
Produced by FastQC (version 0.11.5)