FastQCFastQC Report
Thu 26 May 2016
SRR936461_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936461_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1750167
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT146230.8355202674944735No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT75450.43110171772179456No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT66590.38047797724445725No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC55350.31625553447185323No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT47030.26871721384302183No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42460.24260541993992574No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT42140.240777022992663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38040.21735068710585903No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC36060.2060374809946708No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC34300.19598129778472567No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA32110.18346820617689627No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG29380.16786969472056096No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC24800.1417007634128629No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA23450.13398721379159817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22080.12615938936112953No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT17800.1017045801914903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACG3300.023.0285024
TAAGGTG8550.022.7759955
GGTAAGG7350.021.9723263
GTACATG48350.021.5268441
GTAAGGT7300.021.4709244
TACATGG47800.021.0677052
GAGTACT59000.020.32999612-13
ACATGGG48300.019.6683223
GTATAGC7750.019.6237051
AGTACTT62750.019.26646212-13
GTACTTT63300.019.13630914-15
AGGCGAC4051.8189894E-1218.7639645
GTAATAC3357.3123374E-1018.432453
AGAGTAC87500.018.29599210-11
GGCGACA3951.8189894E-1118.0370796
GGTGTGC13250.017.5646638
CATGGGG29100.016.9746174
TACTTTT71500.016.70912414-15
GTAGCCC11650.016.308673
ACTTTTT76050.016.27020316-17
Produced by FastQC (version 0.11.5)