FastQCFastQC Report
Thu 26 May 2016
SRR936460_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936460_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868236
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT137130.7340079090650218No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT82730.4428241399908791No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT62870.33652065370756157No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC54610.2923078240650539No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT41920.22438278675713347No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT38610.20666553904324722No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC36310.1943544605713625No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34750.18600433778173636No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC33060.17695837142630802No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC29510.15795648943709467No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG29220.15640422302107443No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28450.15228268805439998No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA27220.14569893739334858No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA23080.12353899614395611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG55850.022.3850691
TACATGG54350.021.7789672
ACATGGG54200.021.3147093
GTGTAGC12750.020.878711
GAGTACT45150.020.79637112-13
GTACTTT48950.019.0789414-15
AGAGTAC76200.017.47070310-11
GTCTAGA8800.017.2859461
AGTACTT49100.017.2836312-13
CATGGGG33150.017.0661624
TAGCCCT13200.016.9276374
ACCGTGC2253.506669E-516.9072518
GTATAGG5150.016.6146471
TAAGGTG7600.016.264185
CCTACAC8500.016.2200243
ACTTTTT61000.015.85291716-17
GTATAGC8450.015.7516911
GTAAGCG2452.910383E-1115.51292894-95
ACCGGAC1850.003121620315.4221568
GTATATA12400.015.3343071
Produced by FastQC (version 0.11.5)