##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936460_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1868236 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.42025204524482 31.0 31.0 34.0 30.0 34.0 2 31.484124596678363 31.0 31.0 34.0 28.0 34.0 3 31.443189725495067 33.0 31.0 34.0 28.0 34.0 4 35.08936130124888 37.0 35.0 37.0 32.0 37.0 5 35.12701286133015 37.0 35.0 37.0 32.0 37.0 6 35.18290515759251 37.0 35.0 37.0 32.0 37.0 7 35.051941510601445 37.0 35.0 37.0 32.0 37.0 8 35.04400782342274 37.0 35.0 37.0 32.0 37.0 9 36.55086777045298 39.0 37.0 39.0 32.0 39.0 10-11 36.437174157868704 39.0 37.0 39.0 32.0 39.0 12-13 36.35679539415791 39.0 37.0 39.0 32.0 39.0 14-15 37.35801499382305 40.0 37.0 41.0 32.0 41.0 16-17 37.27300485591756 40.0 37.0 41.0 32.0 41.0 18-19 37.15004153650824 39.5 36.5 41.0 31.0 41.0 20-21 37.12645029857042 39.0 36.0 41.0 31.0 41.0 22-23 37.136878317300386 39.0 36.5 41.0 31.0 41.0 24-25 37.25709251936051 39.0 37.0 41.0 31.0 41.0 26-27 37.1916173866685 39.0 36.5 41.0 31.0 41.0 28-29 37.10050577121948 39.0 36.0 41.0 31.0 41.0 30-31 36.942668378084996 39.0 36.0 41.0 30.5 41.0 32-33 36.798691385884865 39.0 36.0 41.0 30.0 41.0 34-35 36.61000135957127 39.0 35.5 41.0 30.0 41.0 36-37 36.41050970005931 39.0 35.0 40.0 30.0 41.0 38-39 36.23106770236737 39.0 35.0 40.0 30.0 41.0 40-41 36.054438786106246 39.0 35.0 40.0 29.5 41.0 42-43 35.80341000815743 38.0 35.0 40.0 28.5 41.0 44-45 35.68836458563051 38.0 35.0 40.0 28.0 41.0 46-47 35.61607848259 38.0 35.0 40.0 27.0 41.0 48-49 35.4126076684102 38.0 35.0 40.0 27.0 41.0 50-51 34.45254534223727 37.5 33.5 39.5 25.0 40.5 52-53 34.578007810576395 38.0 33.5 39.5 26.0 40.5 54-55 35.04578784479048 38.0 34.0 40.0 26.0 41.0 56-57 34.881442440890766 38.0 34.0 40.0 26.0 41.0 58-59 34.666924574839584 38.0 34.0 40.0 26.0 41.0 60-61 34.348359361451124 38.0 34.0 40.0 25.0 41.0 62-63 34.04653962347369 37.0 33.0 40.0 24.0 41.0 64-65 33.70348178709756 37.0 33.0 39.5 23.0 41.0 66-67 33.31108703611321 36.0 32.5 39.0 22.5 41.0 68-69 32.86547898659484 36.0 32.0 39.0 22.0 40.5 70-71 32.44389868303576 35.0 31.5 38.5 21.0 40.0 72-73 32.02105328234762 35.0 31.0 37.5 20.0 40.0 74-75 31.612535300679358 35.0 31.0 37.0 18.5 39.0 76-77 31.159918768292655 35.0 31.0 36.5 18.0 39.0 78-79 30.680020886012258 34.0 30.0 36.0 14.0 38.5 80-81 30.200220689463215 34.0 30.0 35.5 9.5 37.0 82-83 29.79350681605536 34.0 29.0 35.0 7.5 37.0 84-85 29.418523944512366 34.0 29.0 35.0 6.5 36.5 86-87 29.108924407837126 34.0 29.0 35.0 2.0 36.0 88-89 28.739046084113568 34.0 29.0 35.0 2.0 36.0 90-91 28.370683628834904 34.0 29.0 35.0 2.0 35.0 92-93 27.94046897715278 34.0 27.0 35.0 2.0 35.0 94-95 27.448040825677268 33.0 26.5 35.0 2.0 35.0 96-97 26.837660231362634 33.0 25.0 35.0 2.0 35.0 98-99 26.11146584264515 33.0 24.5 35.0 2.0 35.0 100-101 23.496006393196573 29.5 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3663318439608929 1101 2 0.33827061767087585 1101 3 0.46617350876204355 1101 4 0.4727781291135216 1101 5 0.35201761797075193 1101 6 0.36380121037315405 1101 7 0.5698839325374223 1101 8 0.36984095281383134 1101 9 0.528975893591145 1101 10-11 0.5886297274345935 1101 12-13 0.6444325558301571 1101 14-15 0.7255731069655909 1101 16-17 0.7738541967087045 1101 18-19 0.8480362973567068 1101 20-21 0.8152027137382802 1101 22-23 0.6031094259020122 1101 24-25 0.5778859592309544 1101 26-27 0.5012084512432438 1101 28-29 0.5199512195239464 1101 30-31 0.3261845414210782 1101 32-33 0.2664906233818769 1101 34-35 0.46324711761939596 1101 36-37 0.4345283968560665 1101 38-39 0.33785745346425955 1101 40-41 0.32932072874525176 1101 42-43 0.4943216608515755 1101 44-45 0.4963150493857498 1101 46-47 0.6224287662847772 1101 48-49 0.6987881001093896 1101 50-51 0.5888965532325585 1101 52-53 0.6267851537776536 1101 54-55 0.5413759672461609 1101 56-57 0.7018080022886721 1101 58-59 0.6208895460142472 1101 60-61 0.7089540453986132 1101 62-63 0.6610058223714717 1101 64-65 0.31806957824404947 1101 66-67 0.4900267392308493 1101 68-69 0.5492443016220037 1101 70-71 0.411830535303352 1101 72-73 0.5745132487247702 1101 74-75 0.39321148888499735 1101 76-77 0.42146598598688456 1101 78-79 0.48445479842636274 1101 80-81 0.6099387338260449 1101 82-83 0.630607329633353 1101 84-85 0.5255773065064027 1101 86-87 0.617766482602164 1101 88-89 0.6357403661911256 1101 90-91 0.6524099315000669 1101 92-93 0.7532199884184934 1101 94-95 0.7502790494712279 1101 96-97 0.5489367705964021 1101 98-99 0.6081715317578045 1101 100-101 1.0483684901324395 1102 1 0.20763015862432965 1102 2 0.34526930573223424 1102 3 0.3378381570615687 1102 4 0.20129008793882974 1102 5 0.29686195140499905 1102 6 0.24948720290519333 1102 7 0.3335608928765055 1102 8 0.18246836223961083 1102 9 0.33518363402913565 1102 10-11 0.28447630630228815 1102 12-13 0.12490611522061812 1102 14-15 0.40788211897487514 1102 16-17 0.08067375468630189 1102 18-19 0.128947590120859 1102 20-21 0.24621934003760515 1102 22-23 0.05810299235674421 1102 24-25 0.2433542204076673 1102 26-27 0.1876015030143634 1102 28-29 0.0164834124798503 1102 30-31 0.05343880522165989 1102 32-33 0.06681040842578057 1102 34-35 -0.21387910601568905 1102 36-37 -0.02206661777709229 1102 38-39 -0.12561010128394656 1102 40-41 -0.03910254807380653 1102 42-43 -0.055507408681172876 1102 44-45 0.1126221944054322 1102 46-47 0.18754759765020879 1102 48-49 0.17064827772569657 1102 50-51 0.21345062024235517 1102 52-53 0.16066029254039904 1102 54-55 0.4170659965124841 1102 56-57 0.30987957863341364 1102 58-59 0.16302485230146857 1102 60-61 0.2806275240543812 1102 62-63 0.29049984295881615 1102 64-65 0.40249724501163797 1102 66-67 0.2753696093489282 1102 68-69 0.29935341959556894 1102 70-71 0.043628648130074055 1102 72-73 0.44803794849265444 1102 74-75 0.2083845638673374 1102 76-77 0.3768316888832395 1102 78-79 0.2376431877693861 1102 80-81 0.5294266993119656 1102 82-83 0.3875208678309505 1102 84-85 0.6241877868777799 1102 86-87 0.3660149183676289 1102 88-89 0.42325424246522303 1102 90-91 0.4960161228412723 1102 92-93 0.5161999813542089 1102 94-95 0.6481011051781636 1102 96-97 0.5745876939993693 1102 98-99 0.7677297616498215 1102 100-101 1.3470483518742924 1103 1 0.3600635252825022 1103 2 0.45712886440682254 1103 3 0.46133404699812175 1103 4 0.5446106630863454 1103 5 0.4315506582052322 1103 6 0.3044053936931661 1103 7 0.4659470820178413 1103 8 0.31509141910746763 1103 9 0.36816787334068124 1103 10-11 0.3140843637255628 1103 12-13 0.3362274532455629 1103 14-15 0.6732483001443796 1103 16-17 0.7537003198102283 1103 18-19 0.9044261854462334 1103 20-21 0.7880028069970066 1103 22-23 0.6462249334959367 1103 24-25 0.5611326951755302 1103 26-27 0.4740052138331663 1103 28-29 0.48081419580958595 1103 30-31 0.47676574312637143 1103 32-33 0.5925597680661312 1103 34-35 0.4683746822343622 1103 36-37 0.6154120232882505 1103 38-39 0.41997509268621513 1103 40-41 0.6343999986626514 1103 42-43 0.6353555004465647 1103 44-45 0.5795084946295574 1103 46-47 0.6395800452615958 1103 48-49 0.7873705674688338 1103 50-51 0.5795307125717528 1103 52-53 0.7653809438122963 1103 54-55 0.7611884505928188 1103 56-57 0.8446470643723387 1103 58-59 0.8157769694088657 1103 60-61 0.8587891772931044 1103 62-63 0.8282969894378169 1103 64-65 0.8835588534905199 1103 66-67 0.8590615114131097 1103 68-69 0.8304876402844101 1103 70-71 0.8011124431104335 1103 72-73 0.915876824530784 1103 74-75 0.9051452751892874 1103 76-77 0.7393699961121172 1103 78-79 0.7496926081785595 1103 80-81 0.8787336978548197 1103 82-83 0.8312251686954362 1103 84-85 0.8755256811400294 1103 86-87 0.7193868529725336 1103 88-89 0.6178913121010652 1103 90-91 0.9061969019237281 1103 92-93 0.887750697184817 1103 94-95 0.8811593452346145 1103 96-97 1.00165959233901 1103 98-99 1.1301206391385676 1103 100-101 1.5269073257306296 1104 1 0.1527964377455575 1104 2 0.13856284105205674 1104 3 0.33694247844697145 1104 4 0.4365701620941991 1104 5 0.32974220778184105 1104 6 0.24318476804795353 1104 7 0.18691442973108252 1104 8 0.2180350454312645 1104 9 0.5007359806786837 1104 10-11 0.44638534011030373 1104 12-13 0.49877844731842913 1104 14-15 0.5072665124855504 1104 16-17 0.3984469051972539 1104 18-19 0.4598850034611104 1104 20-21 0.5563429851003932 1104 22-23 0.29722015207297403 1104 24-25 0.5458469354060611 1104 26-27 0.13127941682213873 1104 28-29 -0.09255043613260483 1104 30-31 0.10129650519530742 1104 32-33 0.05384316647033671 1104 34-35 0.22353981700182857 1104 36-37 0.33751271778853464 1104 38-39 0.18705197655238948 1104 40-41 0.3353221733055918 1104 42-43 0.38763415360807585 1104 44-45 0.3314507718314843 1104 46-47 0.3976342746541235 1104 48-49 0.4457257563619734 1104 50-51 0.3347204700192634 1104 52-53 0.2049049281116666 1104 54-55 0.3010216153529157 1104 56-57 0.46363259789455924 1104 58-59 0.3628506091113266 1104 60-61 0.3333077184726676 1104 62-63 0.4439445920530929 1104 64-65 0.5034001507700836 1104 66-67 0.4414926161493469 1104 68-69 0.1713306565285464 1104 70-71 0.24515745296754687 1104 72-73 0.4794838401252832 1104 74-75 0.5321715710055592 1104 76-77 0.44797977346680895 1104 78-79 0.4560856408244156 1104 80-81 0.7081981213986879 1104 82-83 0.48770461187312364 1104 84-85 0.6090628567638596 1104 86-87 0.5921796558335082 1104 88-89 0.6034173972678012 1104 90-91 0.733011121089298 1104 92-93 0.6148204495188416 1104 94-95 0.44418054204560775 1104 96-97 0.4819062734027426 1104 98-99 0.45423169003856856 1104 100-101 1.084363394849408 1105 1 0.2583179675507914 1105 2 0.22092510504234397 1105 3 0.37160677221417515 1105 4 0.36887726059001125 1105 5 0.37987151634351335 1105 6 0.28961857086032694 1105 7 0.37881624760181154 1105 8 0.3610783492399108 1105 9 0.29055745493685947 1105 10-11 0.40038661572449996 1105 12-13 0.4293145065139967 1105 14-15 0.580719358338257 1105 16-17 0.6418610849986166 1105 18-19 0.5722201847481401 1105 20-21 0.49365122626698366 1105 22-23 0.3344567604333051 1105 24-25 0.2520274187497762 1105 26-27 0.3755065195271641 1105 28-29 0.3015262919009061 1105 30-31 0.15364910166865542 1105 32-33 0.21241193215696086 1105 34-35 0.08962040802570215 1105 36-37 0.19491573837548515 1105 38-39 -0.012746519781224208 1105 40-41 0.1313391723910584 1105 42-43 -0.007183904687011022 1105 44-45 0.1028926175199274 1105 46-47 0.35524922151088845 1105 48-49 0.5067008983523706 1105 50-51 0.6992770314196406 1105 52-53 0.6952631162414704 1105 54-55 0.5183663336233266 1105 56-57 0.5720242596511582 1105 58-59 0.6752215565962629 1105 60-61 0.39908207774362836 1105 62-63 0.7127709466601857 1105 64-65 0.8071599995278689 1105 66-67 0.5566147895453142 1105 68-69 0.5969687949832903 1105 70-71 0.6596430041063854 1105 72-73 0.548490612206848 1105 74-75 0.542622490182783 1105 76-77 0.44837960782975017 1105 78-79 0.7423632290942273 1105 80-81 0.8726352747065462 1105 82-83 0.6300913318296182 1105 84-85 0.8254132104696659 1105 86-87 0.7321339329365948 1105 88-89 0.590409927936161 1105 90-91 0.6159787395264225 1105 92-93 0.7208044698001466 1105 94-95 0.7512767284170181 1105 96-97 0.9319631257925707 1105 98-99 1.001658853311163 1105 100-101 1.406963254033812 1106 1 0.09176581997626343 1106 2 -0.014131157986714271 1106 3 -0.015587903813322868 1106 4 0.20780595658320777 1106 5 0.3191952831135296 1106 6 0.12484689380256242 1106 7 0.30641245569385234 1106 8 0.28432283183060036 1106 9 0.30306735983377564 1106 10-11 0.4523459369356857 1106 12-13 0.47879037071123065 1106 14-15 0.3224806137568166 1106 16-17 0.19367133047932583 1106 18-19 0.28571270363776335 1106 20-21 0.10179542227866278 1106 22-23 -0.02930088020016086 1106 24-25 -0.2178968539937216 1106 26-27 -0.2066962893649844 1106 28-29 -0.16523645025674938 1106 30-31 -0.20281648554506404 1106 32-33 -0.1635916167294269 1106 34-35 -0.14752070521238636 1106 36-37 -0.13152374652165832 1106 38-39 -0.09519926156919922 1106 40-41 -0.14573434645517125 1106 42-43 -0.016592753115162395 1106 44-45 -0.1737033366210028 1106 46-47 -0.18645863184680422 1106 48-49 -0.07896400351480537 1106 50-51 -0.15176095355866437 1106 52-53 -0.20666454144529922 1106 54-55 -0.037171206940548984 1106 56-57 0.009830347084275104 1106 58-59 0.06297817981799625 1106 60-61 0.17392870252443515 1106 62-63 -0.018736028155259987 1106 64-65 -0.2800740148123353 1106 66-67 -0.2766622887953858 1106 68-69 0.006749850551976522 1106 70-71 -0.1590690539101942 1106 72-73 -0.009574987543700786 1106 74-75 0.07806373827212809 1106 76-77 -0.13114325339561006 1106 78-79 -0.013228316262566153 1106 80-81 0.016949020051448827 1106 82-83 0.02539174956311996 1106 84-85 0.21394153164388285 1106 86-87 0.3614932930440915 1106 88-89 0.26881198879763346 1106 90-91 0.4354215097151304 1106 92-93 0.36617922448819584 1106 94-95 0.46402507411841754 1106 96-97 0.2843671025383614 1106 98-99 0.34468431914268294 1106 100-101 0.8717315557920173 1107 1 0.25510514527279327 1107 2 0.3567866855693893 1107 3 0.3659402914247245 1107 4 0.42708754439559016 1107 5 0.43185412077262697 1107 6 0.4240866667602887 1107 7 0.48999004438371685 1107 8 0.4224529053044819 1107 9 0.3708776155601399 1107 10-11 0.4169028367812544 1107 12-13 0.2959995231062109 1107 14-15 0.20529831077548977 1107 16-17 0.290085878324156 1107 18-19 0.5454508932494662 1107 20-21 0.3638200535085687 1107 22-23 0.4015566518001563 1107 24-25 0.33937732487979844 1107 26-27 0.19189272561466453 1107 28-29 0.28207586622622927 1107 30-31 0.22785116724407573 1107 32-33 0.34564963186102915 1107 34-35 0.38840079191565025 1107 36-37 0.36308220430268534 1107 38-39 0.5549006987386491 1107 40-41 0.37249524148030844 1107 42-43 0.37750662956533176 1107 44-45 0.44315681285191033 1107 46-47 0.3907759456211579 1107 48-49 0.5029331196326581 1107 50-51 0.4750706656996826 1107 52-53 0.4157915703177082 1107 54-55 0.5687651175345181 1107 56-57 0.7417777167097555 1107 58-59 0.798643532356131 1107 60-61 0.8357975242987834 1107 62-63 0.7660072314779782 1107 64-65 0.7479731667686096 1107 66-67 0.7047617046771109 1107 68-69 0.5895399485040045 1107 70-71 0.7118597385250922 1107 72-73 0.6608098921922334 1107 74-75 0.6733508474760477 1107 76-77 0.6440859234143872 1107 78-79 0.8063636476152105 1107 80-81 0.8312637227982549 1107 82-83 0.8105748384964286 1107 84-85 0.7739232401109355 1107 86-87 0.858144021036054 1107 88-89 1.0429104718945403 1107 90-91 1.0050936667350818 1107 92-93 0.9952145276202451 1107 94-95 0.9441050186914417 1107 96-97 1.139130614638944 1107 98-99 1.3520868464990983 1107 100-101 1.781471363075962 1108 1 0.1384988200061521 1108 2 0.19146163685234896 1108 3 0.3595550167616892 1108 4 0.33233129803423367 1108 5 0.2086084868826248 1108 6 0.2748338719129748 1108 7 0.3645073745952345 1108 8 0.37038236534808533 1108 9 -0.11127315617461164 1108 10-11 0.10846697219725598 1108 12-13 0.048919650323220765 1108 14-15 0.5109704374703128 1108 16-17 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94-95 -3.3904901565096957 2316 96-97 -5.2007280914814515 2316 98-99 -7.3324757570623404 2316 100-101 -8.276818950788758 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3963.0 3 1467.0 4 1485.0 5 1666.0 6 1735.0 7 2011.0 8 2640.0 9 3352.0 10 4241.0 11 5346.0 12 6487.0 13 7809.0 14 9016.0 15 10294.0 16 12120.0 17 14440.0 18 12761.0 19 10703.0 20 11047.0 21 12333.0 22 14172.0 23 15797.0 24 18322.0 25 21336.0 26 25080.0 27 29983.0 28 35815.0 29 43133.0 30 52715.0 31 65377.0 32 82154.0 33 104632.0 34 138015.0 35 185219.0 36 258204.0 37 337652.0 38 267870.0 39 37835.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.81965592883946 18.601055837237148 14.642978628804377 22.936309605119014 2 15.237913193769586 20.220198674610224 39.97821893186027 24.563669199759914 3 20.649714822281872 23.40288164937327 29.570953950784762 26.376449577560095 4 12.710891202544964 16.33163744139643 38.261752702442976 32.695718653615636 5 13.818670110411869 37.3033847432236 34.55802592874574 14.319919217618782 6 31.733898473884913 36.16837061968775 17.384872066985864 14.71285883944147 7 28.17755740182321 32.250072185862926 20.94600711733859 18.626363294975274 8 25.55770438895801 34.069066278626984 19.976188474171778 20.39704085824323 9 24.97233265946084 18.008465724102244 21.2406431893346 35.77855842710231 10-11 25.066589467696677 26.064480778902617 27.530841649094263 21.33808810430645 12-13 26.426437120137088 23.46903531553723 27.40474979249779 22.699777771827893 14-15 22.74677203718386 25.325393593101968 26.536035287020326 25.391799082693844 16-17 22.074544364370322 28.369689830080453 27.018495899634164 22.53726990591506 18-19 21.79290425220542 28.050132808615007 28.34293034798026 21.81403259119931 20-21 23.008200245091874 26.41520747417782 28.09653343298333 22.48005884774698 22-23 22.208363033188803 26.461908747589995 28.394810584292408 22.934917634928798 24-25 22.964092430196978 26.46672479179488 28.314584544087605 22.254598233920536 26-27 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28.24927979342838 22.29484951938054 52-53 22.79199961246835 27.751253924493952 26.986949443445134 22.469797019592562 54-55 22.314742777067213 27.0060996122808 27.54722576166202 23.131931848989968 56-57 22.96539473532782 26.913805198082937 27.186344708014985 22.93445535857426 58-59 23.646112184361378 26.774783918053995 27.069405836875593 22.50969806070903 60-61 22.70190237042882 27.13170244462014 28.156918394362744 22.0094767905883 62-63 22.337024522431502 27.27384162456927 27.89093707135927 22.498196781639958 64-65 22.151201193003416 27.987540051364235 27.28221853244736 22.57904022318499 66-67 22.531376581876188 27.218293226351648 26.944745902878793 23.30558428889338 68-69 22.4369614754015 27.189654332578765 27.354680992172607 23.018703199847124 70-71 23.13976439857135 26.985610461420702 26.994308685243396 22.88031645476455 72-73 22.769947019243535 26.649331208963474 27.937792582694094 22.642929189098897 74-75 22.191520411484564 28.078184422859493 27.114088678178643 22.616206487477303 76-77 22.465699811647447 27.13917743795556 27.032472879481134 23.36264987091586 78-79 22.664806079868 27.243364699728755 27.264725935082723 22.827103285320522 80-81 23.21170372281682 27.07060038404279 26.906870961300754 22.810824931839633 82-83 22.86229309438068 26.97566178350001 27.67340995984 22.488635162279312 84-85 22.621531267338142 27.50828818446272 27.319627775073112 22.55055277312602 86-87 22.548810221576325 27.667547191020258 27.02703932384294 22.756603263560475 88-89 22.387178498296894 27.60820477190545 26.55770470646832 23.44691202332934 90-91 22.632828021532873 26.977649461049218 26.93867981214274 23.450842705275168 92-93 23.174056893275658 27.339515744163073 27.099585314351955 22.386842048209317 94-95 22.71073830677022 27.21501131689226 27.201895713285413 22.872354663052114 96-97 21.86648251569499 27.285981373450408 28.017687058442505 22.829849052412097 98-99 22.342762164782677 27.677167704563576 26.78333514919562 23.19673498145813 100-101 22.20800537932024 27.07642939053287 27.104830359752746 23.610734870394147 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 107.0 1 74.5 2 66.0 3 78.5 4 78.0 5 105.0 6 173.0 7 317.5 8 497.0 9 629.0 10 628.5 11 599.0 12 662.0 13 792.5 14 930.0 15 1035.0 16 1151.5 17 1284.0 18 1463.5 19 1685.5 20 1943.5 21 2361.0 22 2917.0 23 3677.0 24 4613.5 25 5988.5 26 8154.5 27 10873.5 28 13490.5 29 16418.5 30 20132.0 31 24379.0 32 28905.0 33 32491.0 34 35511.5 35 39016.5 36 43382.5 37 47377.0 38 50459.5 39 52925.0 40 55514.0 41 58478.0 42 60308.0 43 62270.5 44 65137.5 45 67981.0 46 70745.5 47 82396.0 48 103530.0 49 100762.0 50 78474.5 51 67315.0 52 63996.5 53 61781.5 54 58555.0 55 53894.5 56 48846.5 57 43532.0 58 39158.5 59 34806.5 60 28883.5 61 23787.5 62 19538.5 63 14839.5 64 10583.5 65 7997.0 66 6096.5 67 4757.5 68 3828.5 69 3092.0 70 2455.0 71 1819.0 72 1362.0 73 1139.0 74 894.0 75 688.5 76 540.0 77 352.5 78 242.5 79 182.5 80 115.5 81 68.5 82 50.5 83 30.5 84 22.0 85 19.0 86 13.5 87 9.0 88 3.5 89 4.0 90 6.5 91 4.5 92 2.0 93 1.0 94 1.0 95 2.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0787373757919235 2 0.02992127332949371 3 0.01488034702253891 4 0.020821780545926746 5 0.026870266925591843 6 0.03307933258967283 7 0.08173485576768673 8 0.0574338573927491 9 0.07568636938802165 10-11 0.06417818733821637 12-13 0.04207177251696252 14-15 0.04927107710160814 16-17 0.06136804986093834 18-19 0.057567673463095666 20-21 0.019537146270599645 22-23 0.012926632395478943 24-25 0.02282902160112534 26-27 0.024729209800046677 28-29 0.0064231713766355 30-31 9.099492783566958E-4 32-33 0.008965676713220386 34-35 0.011133497052834866 36-37 0.004175061394813075 38-39 0.008938913499151071 40-41 0.0038271396119119853 42-43 0.00192695141299065 44-45 2.6763214069314584E-4 46-47 4.549746391783479E-4 48-49 9.902389205646395E-4 50-51 0.0011775814190498417 52-53 8.028964220794376E-5 54-55 1.3381607034657292E-4 56-57 0.0035595074712188395 58-59 0.004228587822951704 60-61 0.004977957816892512 62-63 0.0025960317647235145 64-65 5.352642813862917E-4 66-67 1.8734249848520207E-4 68-69 0.002836900691347346 70-71 0.002060767483337223 72-73 0.020768254117788116 74-75 0.014345082741152617 76-77 0.024729209800046677 78-79 0.01961743591280759 80-81 0.011508182049805271 82-83 0.0044694567495755354 84-85 0.003452454614941581 86-87 0.0016593192722975042 88-89 0.0016325560582281895 90-91 0.006182302450011669 92-93 0.010410890272963373 94-95 0.012418131328161967 96-97 0.010892628126211036 98-99 0.023364285882511632 100-101 0.018306038423411174 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1868236.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.21048200023883 #Duplication Level Percentage of deduplicated Percentage of total 1 80.47216486527239 51.67156493601824 2 12.082692269807046 15.516709890097404 3 3.511842523481286 6.764913034250053 4 1.4537705295243868 3.7338922567401323 5 0.765278575085167 2.4569453085337263 6 0.43291594534074307 1.6678644909550893 7 0.2560193183289018 1.1507386681889953 8 0.19646298221911063 1.0091986226794756 9 0.1375364663400078 0.7948154516671371 >10 0.6387558324213238 7.126330539029733 >50 0.03473777113032011 1.5211876903210373 >100 0.01449241953279346 1.7496045447811595 >500 0.001332200606572332 0.5335100403660937 >1k 0.0016652507582154122 2.4991943428152505 >5k 2.4978761373231184E-4 1.0703882671780287 >10k+ 8.32625379107706E-5 0.7331419163784181 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13713 0.7340079090650218 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8273 0.4428241399908791 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6287 0.33652065370756157 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5461 0.2923078240650539 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4192 0.22438278675713347 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3861 0.20666553904324722 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3631 0.1943544605713625 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3475 0.18600433778173636 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3306 0.17695837142630802 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2951 0.15795648943709467 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2922 0.15640422302107443 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2845 0.15228268805439998 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2722 0.14569893739334858 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2308 0.12353899614395611 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 5.3526428138629166E-5 0.0 0.0 0.0 0.0 7 5.3526428138629166E-5 0.0 0.0 0.0 0.0 8 5.3526428138629166E-5 0.0 0.0 0.0 0.0 9 5.3526428138629166E-5 0.0 0.0 0.0 0.0 10-11 5.3526428138629166E-5 0.0 0.0 0.0 0.0 12-13 1.0705285627725833E-4 0.0 0.0 0.0 5.3526428138629166E-5 14-15 1.6057928441588748E-4 0.0 0.0 0.0 8.028964220794374E-5 16-17 2.4086892662383125E-4 0.0 0.0 0.0 1.0705285627725833E-4 18-19 2.6763214069314584E-4 0.0 0.0 5.3526428138629166E-5 1.0705285627725833E-4 20-21 2.6763214069314584E-4 0.0 0.0 5.3526428138629166E-5 1.0705285627725833E-4 22-23 3.7468499697040415E-4 0.0 0.0 1.3381607034657292E-4 1.3381607034657292E-4 24-25 4.817378532476625E-4 0.0 0.0 2.6763214069314584E-4 1.605792844158875E-4 26-27 5.352642813862917E-4 0.0 0.0 4.0144821103971874E-4 1.605792844158875E-4 28-29 5.352642813862917E-4 0.0 0.0 6.155539235942354E-4 1.605792844158875E-4 30-31 5.620274954556063E-4 0.0 0.0 0.0011240549909112124 1.605792844158875E-4 32-33 6.4231713766355E-4 0.0 0.0 0.0024622156943769416 2.1410571255451666E-4 34-35 7.226067798714938E-4 0.0 0.0 0.004228587822951704 2.1410571255451666E-4 36-37 8.028964220794375E-4 0.0 0.0 0.005968196737457152 2.1410571255451666E-4 38-39 8.028964220794375E-4 0.0 0.0 0.00810925386300232 2.1410571255451666E-4 40-41 8.564228502180667E-4 0.0 0.0 0.011240549909112125 2.1410571255451666E-4 42-43 8.564228502180667E-4 0.0 0.0 0.018011643068648713 2.1410571255451666E-4 44-45 8.564228502180667E-4 0.0 0.0 0.027806979418017852 2.1410571255451666E-4 46-47 8.564228502180667E-4 0.0 0.0 0.03987718896327873 2.6763214069314584E-4 48-49 8.564228502180667E-4 0.0 0.0 0.06016370522781918 2.6763214069314584E-4 50-51 8.564228502180667E-4 0.0 0.0 0.08053051113456758 2.6763214069314584E-4 52-53 8.564228502180667E-4 0.0 0.0 0.10250310988547486 3.21158568831775E-4 54-55 8.564228502180667E-4 0.0 0.0 0.1424338252768922 3.21158568831775E-4 56-57 8.564228502180667E-4 0.0 0.0 0.18249835673865614 3.21158568831775E-4 58-59 8.564228502180667E-4 0.0 0.0 0.2251589199651436 3.7468499697040415E-4 60-61 9.63475706495325E-4 0.0 0.0 0.27360033743060297 3.7468499697040415E-4 62-63 9.63475706495325E-4 0.0 0.0 0.33702915477487855 3.7468499697040415E-4 64-65 9.63475706495325E-4 0.0 0.0 0.3886018682864477 3.7468499697040415E-4 66-67 9.63475706495325E-4 0.0 0.0 0.47065788262296626 3.7468499697040415E-4 68-69 9.63475706495325E-4 0.0 0.0 0.5766402103374519 4.0144821103971874E-4 70-71 9.902389205646395E-4 0.0 0.0 0.6544141104228802 4.282114251090333E-4 72-73 0.001017002134633954 0.0 0.0 0.7582553810118208 4.817378532476625E-4 74-75 0.001017002134633954 0.0 0.0 0.8821155357246087 4.817378532476625E-4 76-77 0.001017002134633954 0.0 0.0 0.9847256984663608 4.817378532476625E-4 78-79 0.0010705285627725834 0.0 0.0 1.0880049415598458 4.817378532476625E-4 80-81 0.0010705285627725834 0.0 0.0 1.2413849213910875 4.817378532476625E-4 82-83 0.0010705285627725834 0.0 0.0 1.4192532420957524 5.352642813862917E-4 84-85 0.0010705285627725834 0.0 0.0 1.6270160729158416 5.352642813862917E-4 86-87 0.0010705285627725834 0.0 0.0 1.8989570910741471 5.352642813862917E-4 88-89 0.0010705285627725834 0.0 0.0 2.2034957039688776 5.352642813862917E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 5585 0.0 22.385069 1 TACATGG 5435 0.0 21.778967 2 ACATGGG 5420 0.0 21.314709 3 GTGTAGC 1275 0.0 20.87871 1 GAGTACT 4515 0.0 20.796371 12-13 GTACTTT 4895 0.0 19.07894 14-15 AGAGTAC 7620 0.0 17.470703 10-11 GTCTAGA 880 0.0 17.285946 1 AGTACTT 4910 0.0 17.28363 12-13 CATGGGG 3315 0.0 17.066162 4 TAGCCCT 1320 0.0 16.927637 4 ACCGTGC 225 3.506669E-5 16.907251 8 GTATAGG 515 0.0 16.614647 1 TAAGGTG 760 0.0 16.26418 5 CCTACAC 850 0.0 16.220024 3 ACTTTTT 6100 0.0 15.852917 16-17 GTATAGC 845 0.0 15.751691 1 GTAAGCG 245 2.910383E-11 15.512928 94-95 ACCGGAC 185 0.0031216203 15.422156 8 GTATATA 1240 0.0 15.334307 1 >>END_MODULE