FastQCFastQC Report
Thu 26 May 2016
SRR936460_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936460_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868236
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT154430.8266086297448503No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT80920.43313585649778724No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT67500.36130338993574684No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC56370.3017284754174526No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT48960.26206539216672836No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT43580.2332681738281459No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42910.22968190314285775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40260.21549739968612106No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC39340.21057296829736713No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC37070.19842246910989833No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA32300.1728903628877722No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG29260.15661832873362894No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC27590.14767941523447786No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA24790.1326920153556617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22460.12022035759936112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT59050.022.60134112-13
TATACCG1503.0376223E-522.1637365
GTACTTT62350.021.17489614-15
TACATGG52000.019.8258652
GTACATG54700.019.1982691
ACATGGG51950.018.6510853
AGAGTAC87400.018.6121710-11
GCGTGCC4101.8189894E-1218.5346329
TAGACCG1801.235095E-418.4697825
TACCCCG1559.636138E-418.3846665
AGTACTT63050.018.34263412-13
CGAACGT1300.00757061918.2668174
GTGTAGC11600.018.0240271
ACTTTTT77750.017.16424616-17
TACAGCG4559.094947E-1216.701095
TACTTTT72800.016.01522614-15
TATACTG9750.015.5876835
GTATAGG5800.015.5662051
CATGGGG33000.015.5434014
GGTAAGG6800.015.3664393
Produced by FastQC (version 0.11.5)