##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936460_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1868236 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82278630751147 31.0 31.0 34.0 30.0 34.0 2 31.990628057697208 31.0 31.0 34.0 30.0 34.0 3 32.08212666922166 33.0 31.0 34.0 30.0 34.0 4 35.77824696665732 37.0 35.0 37.0 35.0 37.0 5 35.60098135353349 37.0 35.0 37.0 33.0 37.0 6 35.685067625289314 37.0 35.0 37.0 33.0 37.0 7 35.633781278168286 37.0 35.0 37.0 33.0 37.0 8 35.605041333107806 37.0 35.0 37.0 33.0 37.0 9 37.229852117184336 39.0 37.0 39.0 33.0 39.0 10-11 37.113121147435336 39.0 37.0 39.0 33.0 39.0 12-13 37.009575877993996 39.0 37.0 39.0 33.0 39.0 14-15 38.120042649857936 40.0 37.0 41.0 33.0 41.0 16-17 38.02195145581179 40.0 37.0 41.0 33.0 41.0 18-19 38.17337772101598 40.0 37.5 41.0 33.0 41.0 20-21 38.18902884860371 40.0 37.5 41.0 33.0 41.0 22-23 38.10008772981572 40.0 37.0 41.0 33.0 41.0 24-25 37.95964027028705 40.0 37.0 41.0 33.0 41.0 26-27 37.75634314936657 39.5 37.0 41.0 32.5 41.0 28-29 37.692713340284634 39.5 37.0 41.0 32.5 41.0 30-31 37.7051354860949 40.0 37.0 41.0 33.0 41.0 32-33 37.570260127735466 40.0 36.5 41.0 32.0 41.0 34-35 37.395334690049864 39.0 36.0 41.0 32.0 41.0 36-37 37.198590006829974 39.0 36.0 41.0 31.0 41.0 38-39 37.038749922386685 39.0 36.0 41.0 31.0 41.0 40-41 36.890417752361046 39.0 36.0 41.0 31.0 41.0 42-43 36.63434972883512 39.0 35.0 40.0 30.0 41.0 44-45 36.39374656092699 39.0 35.0 40.0 30.0 41.0 46-47 36.08563800290756 39.0 35.0 40.0 29.5 41.0 48-49 35.74268293727344 38.0 35.0 40.0 28.5 41.0 50-51 35.47458618718406 38.0 34.5 40.0 28.0 41.0 52-53 35.14207011319769 38.0 34.0 40.0 27.0 41.0 54-55 34.971834125881315 38.0 34.0 40.0 26.5 41.0 56-57 34.64560687193695 38.0 33.5 40.0 26.0 41.0 58-59 34.27067351233998 37.5 33.0 40.0 25.5 41.0 60-61 33.816075966847876 37.0 33.0 39.5 24.0 41.0 62-63 33.514192800053095 36.0 32.0 39.0 23.0 40.0 64-65 33.103109564316284 36.0 32.0 39.0 22.5 40.0 66-67 32.674131908388446 35.5 31.0 38.5 22.0 40.0 68-69 32.29059069625037 35.0 31.0 38.0 22.0 40.0 70-71 31.888205772718223 35.0 31.0 37.5 20.5 39.5 72-73 31.408628513742375 34.5 30.0 37.0 19.5 39.0 74-75 30.90975016004402 34.0 30.0 36.5 18.0 39.0 76-77 29.415392648466252 32.5 28.5 35.0 16.5 37.0 78-79 30.200119524514037 34.0 29.5 35.0 17.5 37.5 80-81 29.951225380519375 34.0 29.0 35.0 12.0 37.0 82-83 29.623275110853236 34.0 29.0 35.0 9.5 36.5 84-85 29.224572805577026 34.0 29.0 35.0 7.0 36.0 86-87 28.86823586527612 33.5 29.0 35.0 4.0 36.0 88-89 28.37631701776435 33.0 28.0 35.0 2.0 35.0 90-91 27.857334137657126 33.0 27.0 35.0 2.0 35.0 92-93 27.20337955161982 32.5 26.0 34.0 2.0 35.0 94-95 26.61683668444458 32.0 25.0 34.0 2.0 35.0 96-97 25.853735823525508 32.0 24.5 34.0 2.0 35.0 98-99 24.92581692034625 32.0 19.5 34.0 2.0 35.0 100-101 21.61141927465267 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23902833961704317 1101 2 0.25004611101524077 1101 3 0.2466676642547796 1101 4 0.2226486906232381 1101 5 0.17943625401619556 1101 6 0.1885909103909995 1101 7 0.13066558779519966 1101 8 0.1568575223100197 1101 9 0.13020442637156293 1101 10-11 0.28089405669179257 1101 12-13 0.24928473178699306 1101 14-15 0.4627727701417186 1101 16-17 0.3659991519021659 1101 18-19 0.3463259035687116 1101 20-21 0.15219875296767071 1101 22-23 0.1971776446279705 1101 24-25 0.21431327026620295 1101 26-27 0.1808091288019753 1101 28-29 0.24207967216317883 1101 30-31 0.4412568045386607 1101 32-33 0.28476799293900257 1101 34-35 0.39498679371637735 1101 36-37 0.2730557110895475 1101 38-39 0.39854164525903 1101 40-41 0.3620932453977659 1101 42-43 0.35635869927144626 1101 44-45 0.3125571604920836 1101 46-47 0.2813885567695493 1101 48-49 0.31356541134277904 1101 50-51 0.17655841746051948 1101 52-53 0.26632252629665487 1101 54-55 0.30655190814216127 1101 56-57 0.19585116358318544 1101 58-59 -0.017884026822372334 1101 60-61 0.027767542357210573 1101 62-63 -0.010057091940900875 1101 64-65 0.04915604020872166 1101 66-67 -0.35045762873753006 1101 68-69 -0.32865027043724027 1101 70-71 -0.173092376874326 1101 72-73 0.07849550470122679 1101 74-75 0.029312829486187297 1101 76-77 0.032796957849186015 1101 78-79 -0.002788912586975556 1101 80-81 -0.03800484896670753 1101 82-83 -0.00893217924883416 1101 84-85 6.350632322877914E-4 1101 86-87 0.017694636061396096 1101 88-89 0.039823380924296714 1101 90-91 -0.08184304780978735 1101 92-93 -0.09265644128409889 1101 94-95 -0.1444185127922566 1101 96-97 0.016323103465499145 1101 98-99 -0.0444619186632238 1101 100-101 0.6195340292700529 1102 1 0.18689896228330838 1102 2 0.21038519669492572 1102 3 0.1206660495610663 1102 4 0.07691249120856725 1102 5 0.18756396619704674 1102 6 0.13457940569829674 1102 7 0.0819876182725423 1102 8 0.10270347043798012 1102 9 0.16310002633119325 1102 10-11 0.2215073880067635 1102 12-13 0.2610530736983847 1102 14-15 0.3064022988529871 1102 16-17 0.290491406418262 1102 18-19 0.2682857380626089 1102 20-21 0.1654461795495692 1102 22-23 0.12421619544036844 1102 24-25 0.1854279135198098 1102 26-27 0.26134071624773014 1102 28-29 0.25267483817387415 1102 30-31 0.2703168761906909 1102 32-33 0.26200459602710424 1102 34-35 0.265426545457224 1102 36-37 0.2731585216657848 1102 38-39 0.3182175711849524 1102 40-41 0.31313144299591045 1102 42-43 0.3507053790574943 1102 44-45 0.3001120348487234 1102 46-47 0.3465628932313578 1102 48-49 0.5318013584615571 1102 50-51 0.39097586624445313 1102 52-53 0.3071263410105516 1102 54-55 0.3831079430599118 1102 56-57 0.2904274326315317 1102 58-59 0.4134385082467631 1102 60-61 0.5454036559912865 1102 62-63 0.3655742785303815 1102 64-65 0.20728501386903275 1102 66-67 0.0432072213987027 1102 68-69 0.18116782422418254 1102 70-71 0.19968805001031953 1102 72-73 0.3192419220995504 1102 74-75 0.14117262865865854 1102 76-77 0.09209847667045779 1102 78-79 0.250024186615768 1102 80-81 0.3205139218477058 1102 82-83 0.3890311621579663 1102 84-85 0.4769047423119126 1102 86-87 0.5813961952500151 1102 88-89 0.4212909861316838 1102 90-91 0.38211080727144875 1102 92-93 0.4726061987401238 1102 94-95 0.16699219969134305 1102 96-97 0.28123252924005016 1102 98-99 0.45581812977758673 1102 100-101 1.0495004739163356 1103 1 0.19598610673668304 1103 2 0.23735626289373712 1103 3 0.23250232985563457 1103 4 0.2431789330959262 1103 5 0.25681102086938523 1103 6 0.20803535483544522 1103 7 0.18033918225296475 1103 8 0.0927321559364529 1103 9 0.21779768509791353 1103 10-11 0.2545767771820664 1103 12-13 0.23035154500308153 1103 14-15 0.35832799263039306 1103 16-17 0.4078140333303608 1103 18-19 0.3306950748983155 1103 20-21 0.24918116703641857 1103 22-23 0.14569050100953262 1103 24-25 0.1779806696800037 1103 26-27 0.21693189725388606 1103 28-29 0.07923661327696152 1103 30-31 0.18294678588691227 1103 32-33 0.162241014521733 1103 34-35 0.30171141402014 1103 36-37 0.4870361933703862 1103 38-39 0.5458104968444246 1103 40-41 0.5040929789442643 1103 42-43 0.44859157963382046 1103 44-45 0.4795785433857631 1103 46-47 0.6210584874916449 1103 48-49 0.4720802661256229 1103 50-51 0.5275392994216119 1103 52-53 0.6677913512367084 1103 54-55 0.6872140450992603 1103 56-57 0.4077649658746907 1103 58-59 0.5182737136519222 1103 60-61 0.5412184482991336 1103 62-63 0.6870885248989609 1103 64-65 0.5275283423669919 1103 66-67 0.41267186766534536 1103 68-69 0.6278378723818392 1103 70-71 0.49938963751415955 1103 72-73 0.4656241351302164 1103 74-75 0.15546024488720178 1103 76-77 0.2655740694932014 1103 78-79 0.17375318706663379 1103 80-81 0.39367480730827253 1103 82-83 0.3376888572552943 1103 84-85 0.6854022494832854 1103 86-87 0.6121440765090398 1103 88-89 0.4963631491097473 1103 90-91 0.6061931898667368 1103 92-93 0.5079380830963984 1103 94-95 0.5551734802799508 1103 96-97 0.7560579822291551 1103 98-99 0.6769159790186308 1103 100-101 0.9648611009460417 1104 1 0.26974821908525115 1104 2 0.28441754100580496 1104 3 0.26648504533724804 1104 4 0.22845838945931263 1104 5 0.23448538562433896 1104 6 0.13857849296687164 1104 7 0.12656204303452512 1104 8 0.15270617046644475 1104 9 0.20633296326786876 1104 10-11 0.3059675264512265 1104 12-13 0.2747319660127161 1104 14-15 0.24826632135945204 1104 16-17 0.36037788747726296 1104 18-19 0.2246384584123362 1104 20-21 0.3028751921471269 1104 22-23 0.1971946151076196 1104 24-25 0.33470061111621874 1104 26-27 0.30956312307340283 1104 28-29 0.28433388994824327 1104 30-31 0.2222129255009193 1104 32-33 0.18862919097208675 1104 34-35 0.07970529071136667 1104 36-37 0.22369572582488928 1104 38-39 0.22113814934022002 1104 40-41 0.25007918059537104 1104 42-43 0.2241552480553608 1104 44-45 0.19901626991441645 1104 46-47 0.33992325933085255 1104 48-49 0.41568216824272497 1104 50-51 0.3400675401281106 1104 52-53 0.4681814146888499 1104 54-55 0.4064878549293667 1104 56-57 0.29727616648058586 1104 58-59 0.2828531542567845 1104 60-61 0.41960049902868235 1104 62-63 0.3106136766148708 1104 64-65 0.37926332675320396 1104 66-67 0.34845833013595495 1104 68-69 0.21622485166742678 1104 70-71 0.2717360764113188 1104 72-73 0.4600228271734501 1104 74-75 0.3876400575859904 1104 76-77 0.15378499159146486 1104 78-79 0.22744047862810746 1104 80-81 0.46538821217669124 1104 82-83 0.420546889113222 1104 84-85 0.6916799273856675 1104 86-87 0.6971745322517329 1104 88-89 0.6791773023468366 1104 90-91 0.7230213746144223 1104 92-93 0.7504587179072786 1104 94-95 0.7619658023454114 1104 96-97 0.9765105651784438 1104 98-99 0.9307491775469785 1104 100-101 1.6326205456031033 1105 1 0.01539351881241302 1105 2 0.12396045662770305 1105 3 0.0772406901108269 1105 4 0.13132394269471348 1105 5 0.1706998558930053 1105 6 0.15828243479950288 1105 7 0.07765071308221394 1105 8 0.14198655295791696 1105 9 0.1810192911015207 1105 10-11 0.12645535248893935 1105 12-13 0.23128657864732816 1105 14-15 0.2137286451541982 1105 16-17 0.22170133319396967 1105 18-19 0.14495323710170283 1105 20-21 0.19153126270112608 1105 22-23 0.16436592455210075 1105 24-25 0.13857452613122945 1105 26-27 0.10624463843559795 1105 28-29 0.08463108476176018 1105 30-31 0.1451133733716361 1105 32-33 0.1638672741609284 1105 34-35 0.2923550177355452 1105 36-37 0.2384638921816844 1105 38-39 0.26093994315260716 1105 40-41 0.2544400059049039 1105 42-43 0.2064142298694307 1105 44-45 0.3184327969101659 1105 46-47 0.1959151116262987 1105 48-49 0.28052158584612386 1105 50-51 0.4188328010084845 1105 52-53 0.48422459278362595 1105 54-55 0.3330786876170535 1105 56-57 0.0802258516135339 1105 58-59 0.04228119230511851 1105 60-61 0.4106248100022185 1105 62-63 0.311566772489563 1105 64-65 0.3360383402985718 1105 66-67 0.15046330567396637 1105 68-69 0.3237893322382135 1105 70-71 0.38046220533591324 1105 72-73 0.5030468923422369 1105 74-75 0.3378077630990468 1105 76-77 0.10415502413649591 1105 78-79 0.2190464672672725 1105 80-81 0.28467784046126354 1105 82-83 0.4244504452894482 1105 84-85 0.4148440329837122 1105 86-87 0.6600370289934112 1105 88-89 0.35993044890872383 1105 90-91 0.5608787002185629 1105 92-93 0.38984206126157517 1105 94-95 0.5642511238254926 1105 96-97 0.6892878134585132 1105 98-99 0.7155036398872383 1105 100-101 1.3406319128679343 1106 1 0.02648941428742191 1106 2 0.0783605614227163 1106 3 0.04212913646043859 1106 4 0.070130967175281 1106 5 0.07653341826452476 1106 6 -0.03234926054916798 1106 7 -0.051551333956609824 1106 8 -0.059395873303841995 1106 9 -0.06270867904154187 1106 10-11 -0.010713807881188586 1106 12-13 0.0686736869317599 1106 14-15 0.107313285612463 1106 16-17 0.06961133967133293 1106 18-19 0.18030688813109919 1106 20-21 -0.09181709875166888 1106 22-23 -0.2125702517478345 1106 24-25 -0.12157588364620864 1106 26-27 0.03833662499226165 1106 28-29 -0.3178794148307844 1106 30-31 -0.15372721123024036 1106 32-33 -0.22048692656010616 1106 34-35 -0.1884335004597304 1106 36-37 -0.3687024715116607 1106 38-39 -0.052682846227099844 1106 40-41 0.06251645483260404 1106 42-43 -0.03084521976386867 1106 44-45 0.04788686631597017 1106 46-47 0.10933445884335669 1106 48-49 -0.2653784699266808 1106 50-51 -0.20407250415878053 1106 52-53 -0.41638468896869796 1106 54-55 -0.3411096733857022 1106 56-57 -0.500156505534946 1106 58-59 -0.5024580015126219 1106 60-61 -0.4322648402042404 1106 62-63 -0.32184194253389364 1106 64-65 -0.4409602388610381 1106 66-67 -0.432389650204442 1106 68-69 -0.27416226275155253 1106 70-71 -0.24497296721422046 1106 72-73 -0.15766819701480372 1106 74-75 -0.26406862218014737 1106 76-77 -0.42266956636733966 1106 78-79 -0.3866506365649798 1106 80-81 -0.34213176210524665 1106 82-83 -0.33078709543514506 1106 84-85 0.2638568382273192 1106 86-87 0.33157979805702453 1106 88-89 0.06626693091435953 1106 90-91 0.051309324150725644 1106 92-93 0.06639494015100311 1106 94-95 -0.0030818832288836973 1106 96-97 0.2541427880875098 1106 98-99 0.28889129143417236 1106 100-101 0.8679447163435299 1107 1 0.012933320910644142 1107 2 0.04282168323501523 1107 3 0.018489618542545827 1107 4 0.04081105236742388 1107 5 0.032640643893500965 1107 6 -0.015442810140292806 1107 7 0.04929928545404039 1107 8 0.08998234872443334 1107 9 0.009294306944681807 1107 10-11 0.11297410210995196 1107 12-13 -0.06431110379274685 1107 14-15 -0.018549012602647963 1107 16-17 0.2060417722684562 1107 18-19 -0.022048028369397343 1107 20-21 0.04899732425621295 1107 22-23 0.0395092461898372 1107 24-25 0.047107875634694096 1107 26-27 -0.00566692047182471 1107 28-29 0.12441703268206084 1107 30-31 -0.0141944975574404 1107 32-33 0.12731872077314677 1107 34-35 0.07474283449469254 1107 36-37 0.10903806254112425 1107 38-39 -0.023937994173081734 1107 40-41 0.13923120713541692 1107 42-43 0.23912277399902138 1107 44-45 0.23640506276135653 1107 46-47 0.10476522523334353 1107 48-49 0.21632072510382727 1107 50-51 0.052866027452509456 1107 52-53 0.1786773270612514 1107 54-55 0.15895463894972295 1107 56-57 -0.08526303122476264 1107 58-59 0.1475079090040623 1107 60-61 0.019127166758856617 1107 62-63 0.14260438349571558 1107 64-65 0.14088519768556296 1107 66-67 0.05171484589854458 1107 68-69 0.13623704188611185 1107 70-71 0.034789736050242936 1107 72-73 0.19611188872972107 1107 74-75 0.1640486322276189 1107 76-77 -0.10511586951280805 1107 78-79 0.1629199700936752 1107 80-81 0.47815392670353773 1107 82-83 0.48450087389694474 1107 84-85 0.4474685558559237 1107 86-87 0.48790888151549794 1107 88-89 0.46105984124876187 1107 90-91 0.4620793124640272 1107 92-93 0.5731310654551081 1107 94-95 0.7687583688754707 1107 96-97 0.9098720505157694 1107 98-99 0.776945472406716 1107 100-101 1.128851082317805 1108 1 -0.008337522665286201 1108 2 0.03737042817249758 1108 3 0.054698248538350924 1108 4 0.04236936595250285 1108 5 0.08525146660348781 1108 6 -0.020186602205129134 1108 7 0.07399184961788308 1108 8 0.11931995490968461 1108 9 0.11852869933187549 1108 10-11 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-1.0035264982030654 2316 62-63 -0.8335871039714959 2316 64-65 -0.7681873472622769 2316 66-67 -0.8013276983431012 2316 68-69 -1.2709367274278733 2316 70-71 -1.0771091982200325 2316 72-73 -0.7706081087730361 2316 74-75 -1.203816950525841 2316 76-77 -0.8123268927996108 2316 78-79 -0.9397679449836396 2316 80-81 -0.6319562539679957 2316 82-83 -0.9455615725632676 2316 84-85 -0.938062088095375 2316 86-87 -1.2955868153436043 2316 88-89 -1.3027681375082878 2316 90-91 -1.1873024660447307 2316 92-93 -1.3401267944679098 2316 94-95 -1.256722478591005 2316 96-97 -1.4422605091494347 2316 98-99 -2.2082541331514918 2316 100-101 -2.9666683854416434 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 11.0 4 21.0 5 53.0 6 109.0 7 256.0 8 669.0 9 1283.0 10 2047.0 11 3327.0 12 4696.0 13 6288.0 14 8228.0 15 10610.0 16 12958.0 17 15849.0 18 14629.0 19 11543.0 20 11236.0 21 11746.0 22 12982.0 23 14390.0 24 17221.0 25 21074.0 26 25769.0 27 31943.0 28 39723.0 29 49304.0 30 60193.0 31 74220.0 32 92268.0 33 116105.0 34 150678.0 35 202320.0 36 281233.0 37 352141.0 38 201423.0 39 9690.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.22825889099946 19.470202008304764 15.451932816659205 22.849606284036568 2 15.591603063651553 20.911129586336376 39.14731898368654 24.34994836632553 3 20.667720167146843 23.396543362144 30.075672168637162 25.86006430207199 4 13.101717341920398 17.06695513843005 37.924009600500156 31.907317919149403 5 13.664984270694996 37.39415438324905 34.670471461629006 14.270389884426946 6 31.776820487347422 36.69868260755065 16.90332484761026 14.621172057491666 7 28.04865124106376 32.55830633817141 20.758886992863857 18.63415542790097 8 25.593715140913677 34.27414951858331 19.664485643141443 20.467649697361576 9 24.835459759901855 17.814826392382976 20.984714993180734 36.364998854534434 10-11 25.12455599827859 26.057976615374074 27.380668181107744 21.436799205239595 12-13 26.55583169585053 23.43247263561489 27.507115088054736 22.50458058047985 14-15 22.72303439829743 25.33103490358799 26.706720578600518 25.239210119514066 16-17 21.86964066638262 28.36030886890093 27.348364981726075 22.421685482990373 18-19 21.641720341010732 27.893209064714853 28.90323951630731 21.561831077967103 20-21 22.779703094176295 26.095104177176808 28.751487700560237 22.37370502808666 22-23 22.019151766239258 26.179629436339646 28.9978776759883 22.803341121432798 24-25 22.850592751665207 26.10732798211789 28.90959707445954 22.132482191757358 26-27 22.386511809152278 25.956342213838685 29.74793545914489 21.90921051786414 28-29 21.685459416492478 27.482931294277602 28.988804641938565 21.842804647291356 30-31 22.248593304385807 26.345567517107305 28.65103885648707 22.75480032201982 32-33 22.178000521917177 26.362518317285495 28.929654932451875 22.529826228345456 34-35 23.101485945462606 26.17216155940102 28.708340013098137 22.018012482038234 36-37 22.74735256585934 25.86670549141859 29.478585009078138 21.907356933643932 38-39 22.29855771418172 26.69893454447159 29.10593802105218 21.896569720294508 40-41 22.447619580335733 26.873061502281704 28.656325514263088 22.02299340311947 42-43 22.097859836314132 26.921344441283267 27.970831059472424 23.009964662930173 44-45 22.688877939611196 26.246132328236033 28.18378871117575 22.881201020977027 46-47 23.33623447194995 26.894491690539507 27.879888579148627 21.889385258361916 48-49 22.955265024287762 26.778680298143957 27.96603728137671 22.30001739619157 50-51 22.035756762803803 27.140310046814264 28.69423744563047 22.12969574475146 52-53 22.899686495206968 27.58341705772246 27.237930172521306 22.278966274549266 54-55 22.308868472882804 26.80893043103335 27.874316218877404 23.00788487720644 56-57 23.03207418486902 26.655048067915 27.52183907553713 22.791038671678848 58-59 23.695923652985147 26.539609029399756 27.409702656217583 22.354764661397507 60-61 22.821527336659557 26.87579083407414 28.50967042680079 21.793011402465513 62-63 22.32877434661914 27.0402417992977 28.28387434552171 22.34710950856145 64-65 22.113459667305964 27.714010008391632 27.686519721670884 22.48601060263152 66-67 22.560340780684527 26.8765446174648 27.280359879476347 23.282754722374328 68-69 22.537533651621448 26.81349420987371 27.647816227555218 23.001155910949628 70-71 23.268560093200243 26.632134974418374 27.38436883358656 22.714936098794823 72-73 22.853349362714344 26.262019359438526 28.322626263491337 22.562005014355787 74-75 22.249051295481145 27.705892098058804 27.431805548158376 22.61325105830167 76-77 22.5071311996206 26.822383482701788 27.310955631600276 23.35952968607734 78-79 22.738554294583537 26.977506187464584 27.486528363305545 22.797411154646333 80-81 23.308257997581332 26.781411488722256 27.14426508762716 22.766065426069247 82-83 22.99796942194641 26.64605038381833 27.91249830695937 22.443481887275883 84-85 22.633334261385052 27.228207560908746 27.62631497789809 22.512143199808108 86-87 22.462555893816607 27.355489759112572 27.292295552958674 22.88965879411215 88-89 22.294823097948534 27.368647077549248 26.734355492976903 23.602174331525315 90-91 22.625224074617016 26.627923010706045 27.16554144058391 23.581311474093024 92-93 23.156947330490983 26.888027700335655 27.365700006906877 22.589324962266485 94-95 22.69538462526935 26.729825187003243 27.502869199057763 23.071920988669643 96-97 21.819390646395938 26.80422722796217 28.37465719642504 23.001724929216852 98-99 22.215254273595818 27.29716486011739 27.039838695857078 23.447742170429716 100-101 21.842838994670004 26.699927376647416 27.338514798882585 24.118718829799995 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 122.0 1 98.5 2 93.5 3 110.0 4 91.5 5 82.0 6 156.0 7 353.5 8 594.0 9 718.5 10 713.5 11 708.5 12 761.5 13 841.5 14 982.5 15 1162.0 16 1336.5 17 1495.5 18 1711.0 19 2002.0 20 2323.0 21 2762.0 22 3379.5 23 4119.5 24 5089.5 25 6549.5 26 8514.0 27 11115.0 28 13942.5 29 17165.5 30 20891.5 31 24855.5 32 29288.0 33 32641.5 34 35547.0 35 39338.0 36 43855.0 37 47420.5 38 50005.0 39 52306.0 40 54829.5 41 57754.5 42 59965.5 43 62114.5 44 64279.0 45 66536.5 46 69383.5 47 82698.5 48 105793.5 49 101902.5 50 77619.0 51 66408.5 52 63530.5 53 61315.0 54 58195.0 55 53651.0 56 48001.0 57 42786.0 58 39136.0 59 34882.0 60 28877.5 61 23652.5 62 19313.0 63 14656.0 64 10345.0 65 7678.0 66 5898.0 67 4694.5 68 3737.0 69 3066.0 70 2479.0 71 1816.5 72 1358.5 73 1117.5 74 908.5 75 755.5 76 591.5 77 426.5 78 292.0 79 193.0 80 145.0 81 101.5 82 64.5 83 43.0 84 26.5 85 14.5 86 8.0 87 4.0 88 1.5 89 1.5 90 2.0 91 2.0 92 1.5 93 1.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020982359830342634 2 0.014398609169291246 3 0.009527704208675991 4 0.0 5 0.0035862706852881543 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0012311078471884708 14-15 2.141057125545167E-4 16-17 0.0 18-19 0.0013381607034657292 20-21 2.6763214069314586E-5 22-23 5.352642813862917E-5 24-25 0.0 26-27 0.0012846342753271 28-29 0.0027298478350700874 30-31 0.0014987399878816166 32-33 0.006610513875120702 34-35 0.0016860824863668188 36-37 6.4231713766355E-4 38-39 0.0011240549909112124 40-41 2.4086892662383125E-4 42-43 0.004522983177714165 44-45 0.0013649239175350437 46-47 2.141057125545167E-4 48-49 5.887907095249209E-4 50-51 1.0705285627725835E-4 52-53 1.605792844158875E-4 54-55 0.002114293911475852 56-57 0.01359571274721181 58-59 0.024006603020175182 60-61 0.01876101306258952 62-63 0.012873105967340315 64-65 0.016245270940073952 66-67 0.002943953547624604 68-69 6.690803517328646E-4 70-71 2.6763214069314586E-5 72-73 0.0 74-75 0.0016325560582281895 76-77 0.001980477841129279 78-79 0.007734568866031914 80-81 0.01418450345673673 82-83 0.015790296300895606 84-85 0.028047848344641683 86-87 0.009902389205646395 88-89 0.038512265045743684 90-91 0.044614277853547414 92-93 0.028529586197889346 94-95 0.029680404402869873 96-97 0.04886962889056842 98-99 0.05117126530052948 100-101 0.05716622525205595 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1868236.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.9912677667011 #Duplication Level Percentage of deduplicated Percentage of total 1 79.25341736588413 49.13019817354642 2 12.646031044661399 15.678869933512393 3 3.7997429694862235 7.066526515981813 4 1.5837201338407825 3.927072755377586 5 0.8060091296611674 2.498276388961557 6 0.47487963581522485 1.7663034396465067 7 0.2957624378343145 1.2834281935383494 8 0.2081604875549595 1.032330601797324 9 0.1447411870661548 0.8075420715859358 >10 0.728629072426824 7.876887624487681 >50 0.03834013670203389 1.6216809466184607 >100 0.016781010978875747 1.9384262738416689 >500 0.001806662496516335 0.7769412599023963 >1k 0.0016345994016100155 2.6797220308248058 >5k 2.580946423594761E-4 1.0921869893973888 >10k+ 8.603154745315871E-5 0.8236068009797293 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 15443 0.8266086297448503 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8092 0.43313585649778724 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6750 0.36130338993574684 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5637 0.3017284754174526 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4896 0.26206539216672836 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4358 0.2332681738281459 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4291 0.22968190314285775 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4026 0.21549739968612106 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3934 0.21057296829736713 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3707 0.19842246910989833 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3230 0.1728903628877722 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2926 0.15661832873362894 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2759 0.14767941523447786 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2479 0.1326920153556617 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2246 0.12022035759936112 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 8.028964220794374E-5 2.6763214069314583E-5 24-25 2.6763214069314583E-5 0.0 0.0 2.1410571255451666E-4 5.3526428138629166E-5 26-27 5.3526428138629166E-5 0.0 0.0 4.0144821103971874E-4 1.0705285627725833E-4 28-29 5.3526428138629166E-5 0.0 0.0 6.155539235942354E-4 1.0705285627725833E-4 30-31 5.3526428138629166E-5 0.0 0.0 0.001150818204980527 1.0705285627725833E-4 32-33 5.3526428138629166E-5 0.0 0.0 0.002515742122515571 1.605792844158875E-4 34-35 8.028964220794374E-5 0.0 0.0 0.004255351037021018 1.605792844158875E-4 36-37 1.0705285627725833E-4 0.0 0.0 0.005941433523387838 1.605792844158875E-4 38-39 1.0705285627725833E-4 0.0 0.0 0.007975437792655745 1.605792844158875E-4 40-41 1.0705285627725833E-4 0.0 0.0 0.011026444196557609 1.605792844158875E-4 42-43 1.0705285627725833E-4 0.0 0.0 0.017717247713886254 1.605792844158875E-4 44-45 1.0705285627725833E-4 0.0 0.0 0.027244951922562245 1.605792844158875E-4 46-47 1.0705285627725833E-4 0.0 0.0 0.03931516146782312 1.605792844158875E-4 48-49 1.0705285627725833E-4 0.0 0.0 0.05944109844794769 1.605792844158875E-4 50-51 1.0705285627725833E-4 0.0 0.0 0.0800220100672506 1.605792844158875E-4 52-53 1.605792844158875E-4 0.0 0.0 0.10215518810257376 1.605792844158875E-4 54-55 1.605792844158875E-4 0.0 0.0 0.14173798171109003 1.605792844158875E-4 56-57 1.8734249848520207E-4 0.0 0.0 0.1811601960351904 1.605792844158875E-4 58-59 2.4086892662383125E-4 0.0 0.0 0.22363341676319265 1.8734249848520207E-4 60-61 2.6763214069314584E-4 0.0 0.0 0.2713789906628499 2.1410571255451666E-4 62-63 2.6763214069314584E-4 0.0 0.0 0.33437959658201644 2.1410571255451666E-4 64-65 2.6763214069314584E-4 0.0 0.0 0.38605936294986287 2.1410571255451666E-4 66-67 2.6763214069314584E-4 2.6763214069314583E-5 0.0 0.4685435887114904 2.1410571255451666E-4 68-69 2.6763214069314584E-4 5.3526428138629166E-5 0.0 0.5740977050008671 2.1410571255451666E-4 70-71 2.6763214069314584E-4 5.3526428138629166E-5 0.0 0.6513095775908397 2.1410571255451666E-4 72-73 2.6763214069314584E-4 5.3526428138629166E-5 0.0 0.754107082831077 3.21158568831775E-4 74-75 2.6763214069314584E-4 5.3526428138629166E-5 0.0 0.8771375779077162 3.21158568831775E-4 76-77 2.6763214069314584E-4 5.3526428138629166E-5 0.0 0.9796406877931909 3.21158568831775E-4 78-79 2.6763214069314584E-4 5.3526428138629166E-5 0.0 1.081555006969141 3.21158568831775E-4 80-81 2.6763214069314584E-4 5.3526428138629166E-5 0.0 1.2331686146718082 3.21158568831775E-4 82-83 3.2115856883177497E-4 5.3526428138629166E-5 0.0 1.4104749078810173 3.21158568831775E-4 84-85 3.7468499697040415E-4 5.3526428138629166E-5 0.0 1.6159361022911454 3.21158568831775E-4 86-87 3.7468499697040415E-4 5.3526428138629166E-5 0.0 1.883889401553123 3.479217829010896E-4 88-89 3.7468499697040415E-4 5.3526428138629166E-5 0.0 2.1843600059093173 4.282114251090333E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 5905 0.0 22.601341 12-13 TATACCG 150 3.0376223E-5 22.163736 5 GTACTTT 6235 0.0 21.174896 14-15 TACATGG 5200 0.0 19.825865 2 GTACATG 5470 0.0 19.198269 1 ACATGGG 5195 0.0 18.651085 3 AGAGTAC 8740 0.0 18.61217 10-11 GCGTGCC 410 1.8189894E-12 18.534632 9 TAGACCG 180 1.235095E-4 18.469782 5 TACCCCG 155 9.636138E-4 18.384666 5 AGTACTT 6305 0.0 18.342634 12-13 CGAACGT 130 0.007570619 18.266817 4 GTGTAGC 1160 0.0 18.024027 1 ACTTTTT 7775 0.0 17.164246 16-17 TACAGCG 455 9.094947E-12 16.70109 5 TACTTTT 7280 0.0 16.015226 14-15 TATACTG 975 0.0 15.587683 5 GTATAGG 580 0.0 15.566205 1 CATGGGG 3300 0.0 15.543401 4 GGTAAGG 680 0.0 15.366439 3 >>END_MODULE