FastQCFastQC Report
Thu 26 May 2016
SRR936459_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936459_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2637459
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT79160.30013736706428423No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT44960.17046710489148836No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37840.1434714245794911No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT35410.13425801121458192No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31520.11950896677445982No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC29910.11340460647919076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG96050.024.0392972
GTACATG99900.023.9204241
ACATGGG95500.022.9331633
GTATAGC8400.022.6228371
GTATCGC1100.002874596321.5957576
GAGTACT58600.020.79328712-13
GTGTAGC16050.020.4239831
GTACTTT60100.019.88269214-15
CATGGGG58700.019.504974
AGAGTAC109350.018.78905110-11
AGTACTT60650.018.75893212-13
CTAGTAC5750.018.1769583
GTGTAGG12650.017.6511781
GTATCAA167650.017.5126251
ACTTTTT74150.016.66232716-17
TAGCCCT18000.016.099924
ATAGTAC8300.016.0267813
TAAGGTG10800.015.83628755
TAGTACG2104.0004027E-415.8359864
TACTTTT73300.015.68639214-15
Produced by FastQC (version 0.11.5)