##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936459_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2637459 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.39592767129271 31.0 31.0 34.0 30.0 34.0 2 31.484680140999348 31.0 31.0 34.0 28.0 34.0 3 31.50545468194956 33.0 31.0 34.0 28.0 34.0 4 35.12720501058026 37.0 35.0 37.0 32.0 37.0 5 35.126525947891516 37.0 35.0 37.0 32.0 37.0 6 35.112762700766154 37.0 35.0 37.0 32.0 37.0 7 35.01290712007277 37.0 35.0 37.0 32.0 37.0 8 34.96340758282878 37.0 35.0 37.0 32.0 37.0 9 36.4675170305965 39.0 37.0 39.0 32.0 39.0 10-11 36.33008569991041 39.0 37.0 39.0 32.0 39.0 12-13 36.24108962452118 39.0 35.0 39.0 32.0 39.0 14-15 37.3647178970365 40.0 37.0 41.0 32.0 41.0 16-17 37.25640246919478 40.0 37.0 41.0 31.5 41.0 18-19 37.18725618104395 39.5 36.5 41.0 31.0 41.0 20-21 37.01720235271904 39.0 36.0 41.0 31.0 41.0 22-23 37.13924235409915 39.0 37.0 41.0 31.0 41.0 24-25 37.192825746295966 39.0 37.0 41.0 31.0 41.0 26-27 37.10484693790501 39.0 36.0 41.0 31.0 41.0 28-29 36.97492340165287 39.0 36.0 41.0 30.5 41.0 30-31 36.78459513493859 39.0 36.0 41.0 30.0 41.0 32-33 36.62508232355461 39.0 36.0 41.0 30.0 41.0 34-35 36.46028999882084 39.0 35.0 41.0 30.0 41.0 36-37 36.27547935342312 39.0 35.0 40.0 30.0 41.0 38-39 36.07502751701543 39.0 35.0 40.0 29.0 41.0 40-41 35.878474319411225 38.5 35.0 40.0 28.5 41.0 42-43 35.65557322407666 38.0 35.0 40.0 27.5 41.0 44-45 35.42304695542187 38.0 34.5 40.0 27.0 41.0 46-47 35.32829458202004 38.0 34.0 40.0 26.5 41.0 48-49 35.211083281294606 38.0 34.0 40.0 26.0 41.0 50-51 34.256109384070044 37.5 33.0 39.5 25.0 40.5 52-53 34.361872544748564 37.5 33.5 39.5 25.0 40.5 54-55 34.79667285823211 38.0 34.0 40.0 26.0 41.0 56-57 34.674256358108316 38.0 34.0 40.0 25.5 41.0 58-59 34.43236558369249 38.0 34.0 40.0 25.0 41.0 60-61 34.12223981491276 37.0 33.0 40.0 24.0 41.0 62-63 33.742689649393604 37.0 33.0 40.0 23.0 41.0 64-65 33.375578918951916 36.0 32.5 39.0 22.5 41.0 66-67 32.899109711278925 36.0 32.0 39.0 22.0 41.0 68-69 32.5025152997639 35.0 31.5 39.0 20.5 40.0 70-71 32.099059738938124 35.0 31.0 38.0 20.0 40.0 72-73 31.696149020705157 35.0 31.0 37.0 18.5 39.5 74-75 31.258546767930802 35.0 31.0 37.0 18.0 39.0 76-77 30.79346314009052 34.5 30.0 36.0 14.5 39.0 78-79 30.303680929258046 34.0 30.0 36.0 9.5 37.5 80-81 29.850093025142762 34.0 29.0 35.0 7.0 37.0 82-83 29.44700524254595 34.0 29.0 35.0 6.0 37.0 84-85 29.08730429553597 34.0 29.0 35.0 2.0 36.0 86-87 28.723994951201135 34.0 29.0 35.0 2.0 36.0 88-89 28.316838100611232 34.0 27.5 35.0 2.0 35.5 90-91 27.900833529544915 33.5 27.0 35.0 2.0 35.0 92-93 27.44669831834353 33.0 26.5 35.0 2.0 35.0 94-95 26.94098277925837 33.0 25.0 35.0 2.0 35.0 96-97 26.327066506057534 33.0 24.5 35.0 2.0 35.0 98-99 25.557294350357672 32.5 22.0 35.0 2.0 35.0 100-101 22.604584564158152 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5087766738911768 1101 2 0.5312528634263884 1101 3 0.5111489480893603 1101 4 0.4987111382210401 1101 5 0.35716694232737467 1101 6 0.5241742482022786 1101 7 0.47249506824326915 1101 8 0.4415883170743484 1101 9 0.5929763150211329 1101 10-11 0.7398777401664063 1101 12-13 0.7343144446883798 1101 14-15 0.7954379169788126 1101 16-17 0.7893832803766401 1101 18-19 0.7366625763870402 1101 20-21 0.8162323117194461 1101 22-23 0.7230924471153699 1101 24-25 0.6646329642354871 1101 26-27 0.5858779425382536 1101 28-29 0.6645637840302783 1101 30-31 0.728366345354992 1101 32-33 0.5671261396787131 1101 34-35 0.3987765973367843 1101 36-37 0.29015805447239273 1101 38-39 0.1911045536886462 1101 40-41 0.22611120154102338 1101 42-43 0.2289129084759125 1101 44-45 0.17977058886766173 1101 46-47 0.2566067240905099 1101 48-49 0.39291399083646894 1101 50-51 0.40968038222056435 1101 52-53 0.4549830427816417 1101 54-55 0.3838069965112254 1101 56-57 0.38692952792891333 1101 58-59 0.44232701934761565 1101 60-61 0.42258109021591395 1101 62-63 0.5720145690402845 1101 64-65 0.4448493871747985 1101 66-67 0.4986596966942045 1101 68-69 0.2755245862718425 1101 70-71 0.2813533272083646 1101 72-73 0.3075135938829625 1101 74-75 0.28917590133956494 1101 76-77 0.27015901314300805 1101 78-79 0.3835983335839437 1101 80-81 0.3196421712528341 1101 82-83 0.2729126522356644 1101 84-85 0.3048732641086147 1101 86-87 0.49449394013920056 1101 88-89 0.48653346074566883 1101 90-91 0.38149304115005833 1101 92-93 0.1774880929813527 1101 94-95 0.21916538260694907 1101 96-97 0.34912006890447955 1101 98-99 0.20381308495681694 1101 100-101 0.3721802245104904 1102 1 0.34344575370660024 1102 2 0.27171990275820335 1102 3 0.3130397319864784 1102 4 0.1498643474250585 1102 5 0.24641384321996185 1102 6 0.3167522316521385 1102 7 0.3282560978710123 1102 8 0.41577488594776213 1102 9 0.4944832788958493 1102 10-11 0.45117676797636364 1102 12-13 0.4146082732716536 1102 14-15 0.4205932766310454 1102 16-17 0.4007657387125292 1102 18-19 0.2799483001963523 1102 20-21 0.2602446203622364 1102 22-23 0.18879979474410646 1102 24-25 0.22379867217716054 1102 26-27 0.01273511330614241 1102 28-29 0.01116032681186141 1102 30-31 0.39182159682476225 1102 32-33 0.3065943228799952 1102 34-35 0.357464295888569 1102 36-37 0.12064333103072755 1102 38-39 0.07713555103326541 1102 40-41 0.0400992136833338 1102 42-43 0.14896791717870173 1102 44-45 0.18508466453466355 1102 46-47 0.10615547979418949 1102 48-49 0.261500087640961 1102 50-51 0.2501404910102778 1102 52-53 0.38251505033527167 1102 54-55 0.29862033721072834 1102 56-57 0.28306697179976936 1102 58-59 0.41017484840480734 1102 60-61 0.3323944078136094 1102 62-63 0.41953247789111714 1102 64-65 0.44246270648863373 1102 66-67 0.3283160804165419 1102 68-69 0.3271554748399481 1102 70-71 0.393827114908035 1102 72-73 0.08880938083941459 1102 74-75 0.11121885353928818 1102 76-77 0.13447305778758079 1102 78-79 0.08084611630550498 1102 80-81 0.09923924933628214 1102 82-83 0.05916938723747833 1102 84-85 -0.2583017954445417 1102 86-87 -0.10035369684296569 1102 88-89 -0.1681439621223504 1102 90-91 -0.17144666379365958 1102 92-93 -0.11593066423384002 1102 94-95 -0.24810879557927024 1102 96-97 -0.1527842460510378 1102 98-99 -0.1683793359343504 1102 100-101 -0.04982279456566019 1103 1 0.23163927359907532 1103 2 0.22654725558630062 1103 3 0.2937144333848778 1103 4 0.4253416810295363 1103 5 0.3573052154686849 1103 6 0.3919194453711299 1103 7 0.4420853476414379 1103 8 0.41428974215190806 1103 9 0.32754027931209606 1103 10-11 0.4849521916109296 1103 12-13 0.220144040325863 1103 14-15 0.48473471163455883 1103 16-17 0.49505680020587306 1103 18-19 0.618722500509655 1103 20-21 0.7100722432742401 1103 22-23 0.3413695637746912 1103 24-25 0.4699054919549255 1103 26-27 0.2674426759735624 1103 28-29 0.5158985519474584 1103 30-31 0.3559881337452353 1103 32-33 0.2585298713251163 1103 34-35 0.5874874595562503 1103 36-37 0.5336829825271536 1103 38-39 0.3547274828323026 1103 40-41 0.1714950391392165 1103 42-43 0.28722355637200536 1103 44-45 0.35624859911715845 1103 46-47 0.606528513344962 1103 48-49 0.4990576393461481 1103 50-51 0.48642197564567624 1103 52-53 0.5367888900071023 1103 54-55 0.4763765435802654 1103 56-57 0.4289723407502848 1103 58-59 0.3109666708992975 1103 60-61 0.4006725751830231 1103 62-63 0.6139770105982763 1103 64-65 0.48514390896577453 1103 66-67 0.4004434202170657 1103 68-69 0.4171007272413192 1103 70-71 0.3916823308639579 1103 72-73 0.2580420565448911 1103 74-75 0.39508880654468825 1103 76-77 0.5136657928479664 1103 78-79 0.3554035758644112 1103 80-81 0.2836125116640211 1103 82-83 -0.07542677375795748 1103 84-85 0.06270946228859486 1103 86-87 0.20396478611156255 1103 88-89 0.015267483716293384 1103 90-91 0.14200828144285538 1103 92-93 -0.03990839660927392 1103 94-95 0.17978864879138712 1103 96-97 0.11049260267337857 1103 98-99 0.05900653772357245 1103 100-101 0.0682515388831213 1104 1 0.32466023920266096 1104 2 0.30273390264197886 1104 3 0.2587715486867914 1104 4 0.3755911867257993 1104 5 0.40716818454829706 1104 6 0.3979093633561561 1104 7 0.4160665221122102 1104 8 0.4517021097968339 1104 9 0.44212166702689615 1104 10-11 0.5280741173215944 1104 12-13 0.4705723096647816 1104 14-15 0.35170682450630153 1104 16-17 0.388099141106224 1104 18-19 0.4080887993006712 1104 20-21 0.20593815909119684 1104 22-23 0.4357378728614876 1104 24-25 0.42087558689686944 1104 26-27 0.14174867861493823 1104 28-29 0.13131911091146264 1104 30-31 0.23390083601534428 1104 32-33 0.11677354716364619 1104 34-35 0.062616220272524 1104 36-37 -0.20115009930715644 1104 38-39 -0.07120889512366091 1104 40-41 -0.2818557294808315 1104 42-43 -0.053978854965045286 1104 44-45 0.073673028536696 1104 46-47 -0.19853641411898337 1104 48-49 -0.012203325035997636 1104 50-51 0.030692060154443368 1104 52-53 0.03956672433329089 1104 54-55 -0.14085359965967115 1104 56-57 -0.13487547986008508 1104 58-59 0.058977114258581764 1104 60-61 0.010115112532545822 1104 62-63 0.07791472196561244 1104 64-65 0.10386707851514387 1104 66-67 0.16423869322286322 1104 68-69 0.029639310924324036 1104 70-71 -0.018921533993271566 1104 72-73 -0.19808578299788238 1104 74-75 0.0020572169096730875 1104 76-77 -0.031893743715240674 1104 78-79 0.1477142581689712 1104 80-81 0.2716249144255052 1104 82-83 0.20795870427256347 1104 84-85 0.11380310135653104 1104 86-87 0.005882264742627541 1104 88-89 -0.12624486942397084 1104 90-91 -0.2299047058312098 1104 92-93 -0.24363431441107863 1104 94-95 -0.28653482561042054 1104 96-97 -0.18804248971116522 1104 98-99 -0.2016362607099218 1104 100-101 -0.31404659815797586 1105 1 0.25463270559028217 1105 2 0.12353877828019222 1105 3 0.13943227776608325 1105 4 0.2889568125035922 1105 5 0.0670419159504334 1105 6 0.22461137888281257 1105 7 0.2938086934539683 1105 8 0.4611011050088507 1105 9 0.4539962812018743 1105 10-11 0.4901646253402845 1105 12-13 0.29225801769671733 1105 14-15 0.2859656032061224 1105 16-17 0.25758407471974465 1105 18-19 0.16910211488885807 1105 20-21 0.2729347185539197 1105 22-23 -0.06635580196246593 1105 24-25 -0.00757046124581251 1105 26-27 -0.02699016706385038 1105 28-29 -0.02048644672827038 1105 30-31 -0.14477714697807897 1105 32-33 -0.22708063853015403 1105 34-35 -0.08814550764405737 1105 36-37 -0.02538386955956895 1105 38-39 -0.028786899694821955 1105 40-41 -0.21669256656681313 1105 42-43 -0.3054151719084359 1105 44-45 -0.17008792527193606 1105 46-47 -0.062072721386030594 1105 48-49 -0.020743608920426482 1105 50-51 0.03716932823640917 1105 52-53 0.14328069610214555 1105 54-55 0.06867649197587156 1105 56-57 -0.23133529429150457 1105 58-59 -0.10637539721980005 1105 60-61 -0.12285707391363587 1105 62-63 -0.16480395447537433 1105 64-65 -0.09987266235532388 1105 66-67 0.017845552460947545 1105 68-69 -0.05117845185194625 1105 70-71 -0.05698496789682039 1105 72-73 -0.15345413885158266 1105 74-75 -0.056906096789813176 1105 76-77 -0.13366655958983387 1105 78-79 -0.05293681267173511 1105 80-81 -0.08320280774632138 1105 82-83 -0.17520590957641602 1105 84-85 -0.3139957438979444 1105 86-87 -0.25616252640605097 1105 88-89 -0.1783129433181614 1105 90-91 -0.33330490774091714 1105 92-93 -0.4952535505797222 1105 94-95 -0.25308803233708943 1105 96-97 -0.25999268031455003 1105 98-99 -0.18125907195332402 1105 100-101 -0.13674217813611023 1106 1 0.3588425252038334 1106 2 0.36768558032585474 1106 3 0.3703678586190122 1106 4 0.34675991737595524 1106 5 0.22434457146152909 1106 6 0.40992823207007234 1106 7 0.4757655012771309 1106 8 0.5729560843596317 1106 9 0.707488058960628 1106 10-11 0.7422745503397437 1106 12-13 0.6354701215633654 1106 14-15 0.5893644995962575 1106 16-17 0.5393987185474458 1106 18-19 0.44746704170716356 1106 20-21 0.3499593184084091 1106 22-23 0.5589025138957027 1106 24-25 0.4105176670575261 1106 26-27 0.36000028935880124 1106 28-29 0.3859418935237997 1106 30-31 0.5295112974830047 1106 32-33 0.47378525120515036 1106 34-35 0.4843374674484906 1106 36-37 0.28254321087609213 1106 38-39 0.26068514094027506 1106 40-41 0.36926054739976877 1106 42-43 0.3959408693258055 1106 44-45 0.2033439242776396 1106 46-47 0.2440975704848114 1106 48-49 0.29610429566520224 1106 50-51 0.20388389556570274 1106 52-53 0.11723125031437576 1106 54-55 0.3132566735392217 1106 56-57 0.3854387197884819 1106 58-59 0.22892941950500045 1106 60-61 0.3541234591144189 1106 62-63 0.3422325614769477 1106 64-65 0.4737205426604021 1106 66-67 0.521314460941035 1106 68-69 0.5402108635140692 1106 70-71 0.25936950859730246 1106 72-73 0.047182656881627594 1106 74-75 0.4519738033877907 1106 76-77 0.47408980368665254 1106 78-79 0.2616249664778998 1106 80-81 0.5409802507990342 1106 82-83 0.45078435693761065 1106 84-85 0.4434264069376539 1106 86-87 0.35827066320300816 1106 88-89 0.3576432466332662 1106 90-91 0.3090703276387927 1106 92-93 0.4980089058215391 1106 94-95 0.5637012947979123 1106 96-97 0.35409232118331957 1106 98-99 0.4851433497829234 1106 100-101 0.45186263537896565 1107 1 0.21767932707259519 1107 2 0.2696153955732541 1107 3 0.30905707076596656 1107 4 0.2582246657301681 1107 5 0.25385720684058555 1107 6 0.37386696640857053 1107 7 0.3686328828868142 1107 8 0.5599663306964331 1107 9 0.49029093642930377 1107 10-11 0.5594909179337151 1107 12-13 0.3882772462182871 1107 14-15 0.17217069517917594 1107 16-17 0.3286440466425802 1107 18-19 0.29870868005925644 1107 20-21 0.32715922815570053 1107 22-23 0.436398399434033 1107 24-25 0.3946305360318334 1107 26-27 0.3662701980442975 1107 28-29 0.4300402955199729 1107 30-31 0.3338440910697571 1107 32-33 0.14679637352411135 1107 34-35 0.23573439354745318 1107 36-37 0.34221485028998444 1107 38-39 0.33591939980627217 1107 40-41 0.41679253402113403 1107 42-43 0.3672730258346206 1107 44-45 0.38496090771361224 1107 46-47 0.460883246849221 1107 48-49 0.5487528144590001 1107 50-51 0.5473120384835184 1107 52-53 0.6498217684710923 1107 54-55 0.7096273201733325 1107 56-57 0.5203187367772273 1107 58-59 0.7040302901781885 1107 60-61 0.6602858608982416 1107 62-63 0.7776779851337778 1107 64-65 0.6718982343146749 1107 66-67 0.6996936284925397 1107 68-69 0.7108263073450303 1107 70-71 0.7711780135877504 1107 72-73 0.5863768860173231 1107 74-75 0.74319075496987 1107 76-77 0.5357901381525672 1107 78-79 0.4496180382553483 1107 80-81 0.4886715069043639 1107 82-83 0.44001176721580393 1107 84-85 0.38660237317252566 1107 86-87 0.41879173986471585 1107 88-89 0.32582850358803483 1107 90-91 0.25318863832046645 1107 92-93 0.28766501646073905 1107 94-95 0.30105728863949466 1107 96-97 0.2902869750376418 1107 98-99 0.4836866976274763 1107 100-101 0.26788116372893356 1108 1 0.10338303371492685 1108 2 0.21445082488521905 1108 3 0.24158903939901677 1108 4 0.19311576740827263 1108 5 0.1535570256739831 1108 6 0.06378246607136617 1108 7 0.27886020069536244 1108 8 0.11068636878109572 1108 9 0.26634256230042297 1108 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2316 88-89 -3.1586415740601765 2316 90-91 -3.2850178733492257 2316 92-93 -3.8190083501477226 2316 94-95 -3.562406506409662 2316 96-97 -4.051828054354143 2316 98-99 -5.088090171852791 2316 100-101 -6.003471391699986 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5715.0 3 2403.0 4 2471.0 5 2727.0 6 3163.0 7 3670.0 8 4610.0 9 5806.0 10 7121.0 11 8619.0 12 10241.0 13 11965.0 14 13319.0 15 15059.0 16 16992.0 17 20474.0 18 18124.0 19 16192.0 20 17411.0 21 19095.0 22 21176.0 23 24154.0 24 27573.0 25 32076.0 26 37722.0 27 44615.0 28 52831.0 29 62815.0 30 76161.0 31 93891.0 32 116804.0 33 149170.0 34 195974.0 35 268241.0 36 371948.0 37 473125.0 38 341610.0 39 42392.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.54101378084878 17.862773961772195 14.04162391198337 23.55458834539565 2 15.862105839137888 19.379535430822557 39.66785393187274 25.090504798166812 3 20.309517290754826 22.84880894506563 28.70203869895194 28.139635065227598 4 13.222208738784518 15.689538790586202 36.33941099287822 34.748841477751064 5 14.565025945448188 36.744610469354335 33.72783107279365 14.962532512403829 6 33.68173728061324 34.67429781823313 16.99916395191282 14.644800949240805 7 30.14538320543767 31.433895042896047 19.8586739650234 18.562047786642886 8 26.968919620020813 33.90257647976165 19.322991410722793 19.80551248949475 9 26.318881652533836 17.00028029233846 19.820992189363835 36.859845865763866 10-11 25.833901968229018 25.73441140693612 26.70930891041715 21.72237771441771 12-13 26.703011691431765 22.910564045732606 26.97799823224293 23.408426030592704 14-15 23.39048180349247 24.74737160083035 25.30776301010893 26.554383585568253 16-17 23.220808655330195 27.722497407280777 25.898273625413182 23.15842031197585 18-19 23.1560196879337 26.831493791724842 27.467291858618083 22.545194661723382 20-21 23.99034990783513 25.814982870119046 27.2769881454623 22.91767907658352 22-23 23.466870121441495 25.707899944389958 27.376542572020508 23.448687362148046 24-25 23.57487325571387 25.82248709617854 27.46325259337142 23.139387054736165 26-27 23.284309450668115 25.910775749955828 27.636817316303574 23.168097483072486 28-29 23.010163003512663 26.639622955204796 27.28477054710055 23.065443494181995 30-31 23.348151664536086 25.90600581295157 27.180487198216873 23.565355324295474 32-33 23.156271132265733 25.97026662366988 27.310219338581295 23.563242905483094 34-35 23.885759719062875 26.007669883715707 27.025600592388464 23.08096980483295 36-37 23.744637111275285 25.75087077331571 27.28693518693877 23.217556928470234 38-39 23.37964182923172 26.027931450635972 27.286643914952926 23.305782805179383 40-41 23.588279454921857 25.992902154378143 27.149373251131788 23.269445139568216 42-43 23.39869201742359 26.000684779839112 26.815500746205274 23.785122456532026 44-45 23.541819199344204 25.93615410252395 26.901797329428494 23.620229368703345 46-47 23.99366125837557 26.234647945425703 26.62773864880687 23.143952147391857 48-49 23.813167313758797 26.18288946857559 26.605666858772143 23.398276358893472 50-51 23.404239991142674 26.439467589608412 26.87490236463378 23.28139005461514 52-53 23.83605196105196 26.448967907301242 26.336374253040916 23.378605878605878 54-55 23.456973625687777 26.24649215239368 26.539177742699827 23.757356479218718 56-57 23.679525447570697 26.331327910761697 26.37240953086003 23.616737110807577 58-59 24.040991266235633 26.059272507970764 26.33635581326083 23.563380412532773 60-61 23.58366818427845 26.24090120167174 26.92186432114599 23.253566292903812 62-63 23.295759700634346 26.367503228031236 26.872461912454238 23.46427515888018 64-65 23.24231113315274 26.659098703341716 26.514659042588185 23.583931120917363 66-67 23.412687728314378 26.450432671505265 26.234826918125183 23.902052682055178 68-69 23.450227402903323 26.221773780578012 26.632919747068794 23.69507906944987 70-71 23.65834986413069 26.145177388828255 26.413065456646446 23.783407290394614 72-73 23.48935376291425 26.13406187234754 26.722558487041525 23.654025877696682 74-75 23.34967006676037 26.837161883854698 26.216057534834714 23.597110514550213 76-77 23.607459700052488 26.323648097187117 26.265128598778485 23.803763603981906 78-79 23.552330154784716 26.44388563294886 26.356023621830676 23.647760590435748 80-81 23.754556938632785 26.370591670597328 26.263524720645005 23.61132667012489 82-83 23.664023682914785 26.3851007639136 26.469029596815368 23.48184595635625 84-85 23.57681230758029 26.543581256791633 26.291095123524993 23.588511312103087 86-87 23.57998889611956 26.59161308134199 26.260961668858624 23.56743635367983 88-89 23.518810987166066 26.551793381297912 25.933599848153676 23.995795783382345 90-91 23.648658003173658 26.274940131168073 26.209339025716428 23.867062839941838 92-93 23.71337838450276 26.318374365395364 26.416581287893052 23.551665962208823 94-95 23.505249558318123 26.238880215571854 26.533410641091905 23.72245958501812 96-97 23.214243051571067 26.234156489871115 26.808567279859446 23.743033178698376 98-99 23.150457355903065 26.567288227630414 26.188559189025202 24.093695227441316 100-101 23.314286104540393 26.175107654056895 26.260172333756316 24.250433907646393 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 70.0 1 56.5 2 62.5 3 85.5 4 83.0 5 87.0 6 145.0 7 279.0 8 465.5 9 614.5 10 650.0 11 637.5 12 691.5 13 796.0 14 914.5 15 1031.5 16 1145.0 17 1290.5 18 1418.0 19 1636.5 20 1956.0 21 2291.0 22 2771.5 23 3517.0 24 4461.5 25 5673.5 26 7675.5 27 10191.5 28 12908.0 29 16578.0 30 21349.5 31 26436.5 32 32518.0 33 37438.0 34 41222.0 35 45978.5 36 52559.0 37 58749.0 38 62296.0 39 65817.0 40 70332.0 41 74963.5 42 78083.5 43 82153.0 44 87669.5 45 93491.0 46 98404.5 47 106303.5 48 118944.5 49 118556.5 50 106952.5 51 100777.5 52 100040.0 53 100402.0 54 99829.0 55 94763.5 56 86445.5 57 79147.0 58 74177.0 59 67104.0 60 56625.5 61 47607.5 62 39598.5 63 31216.5 64 22840.0 65 17046.5 66 13424.5 67 10728.0 68 8324.0 69 6425.0 70 5040.0 71 3797.5 72 2948.0 73 2482.0 74 1927.5 75 1369.5 76 996.0 77 650.0 78 400.5 79 282.5 80 206.0 81 143.5 82 99.0 83 65.5 84 43.5 85 29.5 86 20.0 87 16.0 88 14.0 89 12.0 90 8.5 91 4.5 92 4.0 93 3.0 94 1.5 95 2.0 96 1.5 97 1.0 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012170805309201015 2 0.011602076089144894 3 0.014597383314773803 4 0.018161419760458833 5 0.02930851247355883 6 0.04728035582733229 7 0.005801038044572447 8 0.025896137153222098 9 0.03510955051813128 10-11 0.03897690921451291 12-13 0.029744538208935193 14-15 0.02769711301673315 16-17 0.028379588080800495 18-19 0.023336855662969547 20-21 0.05391553006132038 22-23 0.01309972970195935 24-25 0.0023507474428986386 26-27 0.0014028654094717682 28-29 1.8957640668537405E-5 30-31 0.002748857896937924 32-33 0.0019905522701964273 34-35 1.8957640668537405E-5 36-37 1.7061876601683667E-4 38-39 7.583056267414962E-5 40-41 0.0014786959721459177 42-43 0.004360257353763604 44-45 0.0017441029415054415 46-47 0.0010237125961010199 48-49 7.203903454044215E-4 50-51 0.003601951727022107 52-53 0.0037536128523704064 54-55 0.005990614451257821 56-57 0.0010047549554324825 58-59 0.006274979061285881 60-61 0.0034692482423423453 62-63 5.876868607246596E-4 64-65 9.668396740954078E-4 66-67 0.017820182228425165 68-69 0.04432296388304045 70-71 0.05573546356549998 72-73 0.015185070175498463 74-75 0.032796718356569714 76-77 0.027716070657401688 78-79 0.057157286615640276 80-81 0.08311029669086799 82-83 0.049176119894186035 84-85 0.018294123245138596 86-87 0.020587997766031624 88-89 0.02208565137884608 90-91 0.040588308671338585 92-93 0.02576343366854234 94-95 0.014407806908088428 96-97 0.01880597954318911 98-99 0.048285110782764776 100-101 0.06891102383013348 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2637459.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.40720009143684 #Duplication Level Percentage of deduplicated Percentage of total 1 77.91390934019769 44.72819383398799 2 12.745950590182753 14.634186717723773 3 4.000099867876826 6.88903600502805 4 1.76497441697991 4.052889580473311 5 0.9442534763464409 2.7103474126827476 6 0.5802688388342235 1.998696560266919 7 0.38358443288986327 1.5414355803608095 8 0.26310027863177327 1.2083080271621593 9 0.19790719771601825 1.022516828893711 >10 1.0839549444926626 11.310622185122632 >50 0.08103918335618458 3.188543812098764 >100 0.036851769588080376 3.774248896221345 >500 0.0026488147791583834 1.034965453716823 >1k 0.001390627759040686 1.6050803080407885 >5k 6.622036947812792E-5 0.30092879822014307 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7916 0.30013736706428423 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4496 0.17046710489148836 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3784 0.1434714245794911 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3541 0.13425801121458192 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3152 0.11950896677445982 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2991 0.11340460647919076 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 3.791528133707481E-5 0.0 0.0 0.0 3 0.0 3.791528133707481E-5 0.0 0.0 0.0 4 0.0 3.791528133707481E-5 0.0 0.0 3.791528133707481E-5 5 0.0 1.1374584401122444E-4 0.0 0.0 3.791528133707481E-5 6 0.0 1.1374584401122444E-4 0.0 0.0 7.583056267414962E-5 7 0.0 1.1374584401122444E-4 0.0 0.0 7.583056267414962E-5 8 0.0 1.1374584401122444E-4 0.0 0.0 7.583056267414962E-5 9 0.0 1.1374584401122444E-4 0.0 0.0 1.1374584401122444E-4 10-11 0.0 1.1374584401122444E-4 0.0 0.0 1.1374584401122444E-4 12-13 0.0 1.1374584401122444E-4 0.0 0.0 1.5166112534829924E-4 14-15 0.0 1.1374584401122444E-4 0.0 0.0 1.7061876601683664E-4 16-17 0.0 1.1374584401122444E-4 0.0 3.791528133707481E-5 2.0853404735391147E-4 18-19 0.0 1.1374584401122444E-4 0.0 7.583056267414962E-5 3.222798913651359E-4 20-21 0.0 1.1374584401122444E-4 0.0 9.478820334268704E-5 3.4123753203367334E-4 22-23 0.0 1.1374584401122444E-4 0.0 1.7061876601683664E-4 3.7915281337074815E-4 24-25 0.0 1.1374584401122444E-4 0.0 1.8957640668537407E-4 4.1706809470782295E-4 26-27 0.0 1.1374584401122444E-4 0.0 3.222798913651359E-4 4.1706809470782295E-4 28-29 0.0 1.1374584401122444E-4 0.0 7.393479860729589E-4 4.1706809470782295E-4 30-31 0.0 1.1374584401122444E-4 0.0 0.0011374584401122444 4.360257353763603E-4 32-33 0.0 1.1374584401122444E-4 0.0 0.0018388911448481283 4.5498337604489775E-4 34-35 0.0 1.1374584401122444E-4 0.0 0.0023886627242357132 4.928986573819726E-4 36-37 0.0 1.1374584401122444E-4 0.0 0.003128010710308672 4.928986573819726E-4 38-39 0.0 1.1374584401122444E-4 0.0 0.0043412997130950655 4.928986573819726E-4 40-41 0.0 1.1374584401122444E-4 0.0 0.005876868607246596 4.928986573819726E-4 42-43 0.0 1.1374584401122444E-4 0.0 0.008549895941510371 4.928986573819726E-4 44-45 0.0 1.1374584401122444E-4 0.0 0.013573670718672784 5.308139387190474E-4 46-47 0.0 1.3270348467976184E-4 0.0 0.027621282454059003 5.687292200561222E-4 48-49 0.0 1.5166112534829924E-4 0.0 0.037175933351001854 5.687292200561222E-4 50-51 0.0 1.5166112534829924E-4 0.0 0.049232992816191645 5.876868607246595E-4 52-53 0.0 1.5166112534829924E-4 0.0 0.06583988604183041 6.256021420617344E-4 54-55 0.0 1.8957640668537407E-4 0.0 0.08703452830925523 6.445597827302718E-4 56-57 0.0 2.2749168802244888E-4 0.0 0.10843770462403396 6.445597827302718E-4 58-59 0.0 3.033222506965985E-4 0.0 0.13624856348477835 6.445597827302718E-4 60-61 0.0 3.033222506965985E-4 0.0 0.1720595467076455 6.445597827302718E-4 62-63 0.0 3.033222506965985E-4 0.0 0.21628772238734328 7.583056267414963E-4 64-65 0.0 3.033222506965985E-4 0.0 0.2507337554820757 7.583056267414963E-4 66-67 0.0 3.033222506965985E-4 0.0 0.3113034174180528 7.583056267414963E-4 68-69 0.0 3.033222506965985E-4 0.0 0.39086863530390425 7.583056267414963E-4 70-71 0.0 3.222798913651359E-4 0.0 0.44315380826773043 7.583056267414963E-4 72-73 0.0 3.4123753203367334E-4 0.0 0.5306243623123621 7.96220908078571E-4 74-75 0.0 3.4123753203367334E-4 0.0 0.6001420306438887 7.96220908078571E-4 76-77 0.0 3.4123753203367334E-4 0.0 0.6602187939224837 8.151785487471085E-4 78-79 0.0 3.4123753203367334E-4 0.0 0.7380209512261613 8.341361894156459E-4 80-81 0.0 3.601951727022107E-4 0.0 0.8258517004434951 8.341361894156459E-4 82-83 0.0 3.7915281337074815E-4 0.0 0.9371709664491468 8.720514707527208E-4 84-85 0.0 3.7915281337074815E-4 0.0 1.1006237442932763 8.720514707527208E-4 86-87 0.0 4.1706809470782295E-4 0.0 1.329385594240517 8.720514707527208E-4 88-89 0.0 4.360257353763603E-4 0.0 1.6099776337755394 8.720514707527208E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 9605 0.0 24.039297 2 GTACATG 9990 0.0 23.920424 1 ACATGGG 9550 0.0 22.933163 3 GTATAGC 840 0.0 22.622837 1 GTATCGC 110 0.0028745963 21.595757 6 GAGTACT 5860 0.0 20.793287 12-13 GTGTAGC 1605 0.0 20.423983 1 GTACTTT 6010 0.0 19.882692 14-15 CATGGGG 5870 0.0 19.50497 4 AGAGTAC 10935 0.0 18.789051 10-11 AGTACTT 6065 0.0 18.758932 12-13 CTAGTAC 575 0.0 18.176958 3 GTGTAGG 1265 0.0 17.651178 1 GTATCAA 16765 0.0 17.512625 1 ACTTTTT 7415 0.0 16.662327 16-17 TAGCCCT 1800 0.0 16.09992 4 ATAGTAC 830 0.0 16.026781 3 TAAGGTG 1080 0.0 15.8362875 5 TAGTACG 210 4.0004027E-4 15.835986 4 TACTTTT 7330 0.0 15.686392 14-15 >>END_MODULE