FastQCFastQC Report
Thu 26 May 2016
SRR936459_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936459_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2637459
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT84230.31936041470218113No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45920.17410697189984753No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT44010.16686515316446626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41110.15586972157671455No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT34750.13175560264633498No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC32090.12167013781067308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG98750.022.1833041
TACATGG98000.021.573582
TAAGGTG12600.021.1105085
ACATGGG98900.020.6543253
GAGTACT72700.020.41742512-13
GTAAGGT11800.019.7240144
GTACTTT75400.019.46673214-15
CATGGGG57700.018.7689634
AGTACTT75850.018.34836612-13
AGAGTAC125200.017.8499310-11
GTATCAA181150.017.732531
AGGTAAG13150.017.7034782
GGTAAGG13500.017.5944253
GTGTAGC17250.016.803631
ACTTTTT89150.016.65014816-17
ATTGCGC2053.3367355E-416.2178216
ATAGTAC8350.015.9298043
TCAACGC199600.015.8011524
TACTTTT86600.015.74236314-15
CAACGCA201000.015.71472555
Produced by FastQC (version 0.11.5)