##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936459_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2637459 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.97429647247597 31.0 31.0 34.0 30.0 34.0 2 32.09273812408079 33.0 31.0 34.0 30.0 34.0 3 32.23105155378719 34.0 31.0 34.0 30.0 34.0 4 35.938918481765974 37.0 35.0 37.0 35.0 37.0 5 35.79827022903484 37.0 35.0 37.0 35.0 37.0 6 35.836605990841946 37.0 35.0 37.0 35.0 37.0 7 35.76693628223225 37.0 35.0 37.0 33.0 37.0 8 35.73187677988549 37.0 35.0 37.0 33.0 37.0 9 37.36643109902372 39.0 37.0 39.0 34.0 39.0 10-11 37.26653381152086 39.0 37.0 39.0 33.5 39.0 12-13 37.147048162644424 39.0 37.0 39.0 33.0 39.0 14-15 38.289783272460355 40.0 38.0 41.0 33.0 41.0 16-17 38.262363888879406 40.0 38.0 41.0 33.0 41.0 18-19 38.37255290034841 40.0 38.0 41.0 33.5 41.0 20-21 38.367124379942965 40.0 38.0 41.0 34.0 41.0 22-23 38.26873555190811 40.0 38.0 41.0 34.0 41.0 24-25 38.15489321350588 40.0 37.5 41.0 33.0 41.0 26-27 37.96094612276437 40.0 37.0 41.0 33.0 41.0 28-29 38.0130866489299 40.0 37.0 41.0 33.0 41.0 30-31 37.92828229746889 40.0 37.0 41.0 33.0 41.0 32-33 37.77436919398558 40.0 37.0 41.0 33.0 41.0 34-35 37.618571890596215 40.0 37.0 41.0 32.5 41.0 36-37 37.46939269956424 40.0 37.0 41.0 32.0 41.0 38-39 37.28210675502444 39.0 36.0 41.0 31.5 41.0 40-41 37.09016993249942 39.0 36.0 41.0 31.0 41.0 42-43 36.85630601271906 39.0 36.0 41.0 31.0 41.0 44-45 36.62848692624227 39.0 35.0 40.0 30.0 41.0 46-47 36.33346793258208 39.0 35.0 40.0 30.0 41.0 48-49 35.99578116664563 39.0 35.0 40.0 29.5 41.0 50-51 35.71780888347459 38.0 35.0 40.0 28.5 41.0 52-53 35.452378975369854 38.0 34.0 40.0 28.0 41.0 54-55 35.127461886611314 38.0 34.0 40.0 27.0 41.0 56-57 34.777779863118255 38.0 34.0 40.0 26.5 41.0 58-59 34.399534362429904 37.0 33.0 40.0 26.0 41.0 60-61 34.01234388856851 37.0 33.0 39.0 26.0 40.5 62-63 33.67794551498241 36.0 33.0 39.0 25.0 40.0 64-65 33.35073379339735 36.0 32.0 39.0 24.5 40.0 66-67 32.890440761354014 35.0 31.5 38.5 23.0 40.0 68-69 32.43403006454318 35.0 31.0 38.0 22.0 40.0 70-71 31.922026086471867 35.0 31.0 37.0 21.5 39.5 72-73 31.35387829725505 34.0 30.0 36.5 20.0 39.0 74-75 30.81666103624739 34.0 30.0 36.0 18.5 39.0 76-77 29.423432932985875 32.5 28.5 34.5 18.0 36.5 78-79 30.227126184710357 34.0 29.5 35.0 18.0 37.0 80-81 30.080395372970727 34.0 29.5 35.0 17.5 37.0 82-83 29.688610704469717 34.0 29.0 35.0 13.0 36.0 84-85 29.25454424125645 34.0 29.0 35.0 7.5 36.0 86-87 28.84308798733933 33.0 29.0 35.0 6.0 35.5 88-89 28.355425051157194 33.0 27.5 35.0 2.0 35.0 90-91 27.786377153161432 33.0 27.0 34.5 2.0 35.0 92-93 27.19876157316569 32.0 26.0 34.0 2.0 35.0 94-95 26.454650669451162 32.0 25.0 34.0 2.0 35.0 96-97 25.62589200438756 32.0 23.5 34.0 2.0 35.0 98-99 24.47728381749252 31.0 10.5 34.0 2.0 35.0 100-101 20.622649679104015 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2824975409452364 1101 2 0.3735209860064046 1101 3 0.33962205007618707 1101 4 0.2146368613103533 1101 5 0.20565725778831734 1101 6 0.24138836914716677 1101 7 0.31751531391133625 1101 8 0.2312100356133513 1101 9 0.23501801241062026 1101 10-11 0.3063580608719292 1101 12-13 0.40027354469810916 1101 14-15 0.4325284552043698 1101 16-17 0.4653142864159392 1101 18-19 0.42529931109172026 1101 20-21 0.3714297337983936 1101 22-23 0.3963129396067657 1101 24-25 0.3846842639346022 1101 26-27 0.3645668291143309 1101 28-29 0.3660056964173819 1101 30-31 0.44930751219698095 1101 32-33 0.4498716096504154 1101 34-35 0.49118258303509776 1101 36-37 0.5490289471863647 1101 38-39 0.40600717928310814 1101 40-41 0.4382427198095513 1101 42-43 0.5684448884176163 1101 44-45 0.5887639284007165 1101 46-47 0.622891142811703 1101 48-49 0.5890442632372626 1101 50-51 0.5833876533632463 1101 52-53 0.5990959212429416 1101 54-55 0.6409348514336912 1101 56-57 0.6253113232442757 1101 58-59 0.636669260694056 1101 60-61 0.6200892778194671 1101 62-63 0.6250398650077571 1101 64-65 0.6251006623688511 1101 66-67 0.48359752979978765 1101 68-69 0.498285669994587 1101 70-71 0.3810543226027967 1101 72-73 0.5597510772469931 1101 74-75 0.4799583078577001 1101 76-77 0.435041727121682 1101 78-79 0.551193753402508 1101 80-81 0.620109995891351 1101 82-83 0.425294600012581 1101 84-85 0.44027035672631953 1101 86-87 0.36220020096354943 1101 88-89 0.39614837606224995 1101 90-91 0.4598964996716397 1101 92-93 0.4604066154879831 1101 94-95 0.48453138398901885 1101 96-97 0.6522551450723064 1101 98-99 0.014569647711720535 1101 100-101 0.050761751864715876 1102 1 0.2836737529485376 1102 2 0.35298730786210086 1102 3 0.25083520516918156 1102 4 0.16789794539234748 1102 5 0.06763267350533653 1102 6 0.13832487152038198 1102 7 0.17847438212965727 1102 8 0.12837861213940727 1102 9 0.17278425248591844 1102 10-11 0.29881802709048344 1102 12-13 0.22474429880049485 1102 14-15 0.35527264625499555 1102 16-17 0.3224193791762886 1102 18-19 0.2696782124093815 1102 20-21 0.3284210996630321 1102 22-23 0.26928494747721743 1102 24-25 0.23633000366436363 1102 26-27 0.28051433652836266 1102 28-29 0.4067997253263158 1102 30-31 0.3764902864335795 1102 32-33 0.3781949531023372 1102 34-35 0.3410711993226627 1102 36-37 0.32921003895135925 1102 38-39 0.30766443691380374 1102 40-41 0.3383483940753891 1102 42-43 0.3702150808820761 1102 44-45 0.4656600752173219 1102 46-47 0.31482029551002455 1102 48-49 0.4687286661512644 1102 50-51 0.44272999112090616 1102 52-53 0.6663812543016903 1102 54-55 0.6325629545839178 1102 56-57 0.5519751648899671 1102 58-59 0.39587067742886717 1102 60-61 0.4002927453346885 1102 62-63 0.42094404663284024 1102 64-65 0.31546397458713216 1102 66-67 0.2416729644602853 1102 68-69 0.20046667859307377 1102 70-71 0.01506321877866057 1102 72-73 -0.09741922861433139 1102 74-75 0.12138961661467107 1102 76-77 0.2372724376078601 1102 78-79 0.14221045072856953 1102 80-81 0.2201244510220306 1102 82-83 0.27119445829949385 1102 84-85 0.07651596985817477 1102 86-87 0.25900442899026643 1102 88-89 -0.016925200552915243 1102 90-91 0.021703140187060654 1102 92-93 0.3437925879387471 1102 94-95 0.4136033579988272 1102 96-97 0.24844235683601212 1102 98-99 0.16904311462630162 1102 100-101 0.28950051201053384 1103 1 0.22463023130846693 1103 2 0.30525553614338463 1103 3 0.23557323965730603 1103 4 0.131068031533232 1103 5 0.21187782073284467 1103 6 0.1917811512891916 1103 7 0.22417575828664837 1103 8 0.19601779273658337 1103 9 0.17769342485348005 1103 10-11 0.22750348100622375 1103 12-13 0.21483889172048265 1103 14-15 0.301420455238933 1103 16-17 0.26641096526792296 1103 18-19 0.2235691684111245 1103 20-21 0.202212791881756 1103 22-23 0.1650338266289637 1103 24-25 0.23118379294172087 1103 26-27 0.1262980088434844 1103 28-29 0.20954790861480177 1103 30-31 0.3506867867815089 1103 32-33 0.3090093037729389 1103 34-35 0.2171373158654646 1103 36-37 0.14667398274848864 1103 38-39 0.10193071423596933 1103 40-41 0.2753854781858749 1103 42-43 0.3322346095379771 1103 44-45 0.3186101859517265 1103 46-47 0.29359106416885083 1103 48-49 0.33357772101643235 1103 50-51 0.2712896177060671 1103 52-53 0.26250539122469974 1103 54-55 0.3057944609849059 1103 56-57 0.31868112611863353 1103 58-59 0.1879988433684261 1103 60-61 0.07846646966480364 1103 62-63 0.3659911842199506 1103 64-65 0.2935026569589141 1103 66-67 0.20244502316699453 1103 68-69 0.2685681793064134 1103 70-71 -0.024213019873844388 1103 72-73 0.21355920597929057 1103 74-75 0.20924283077215478 1103 76-77 0.13528716194750245 1103 78-79 -0.05730053850938077 1103 80-81 0.26137675070714295 1103 82-83 0.17924359450757876 1103 84-85 0.18165550791935559 1103 86-87 0.04948857824181374 1103 88-89 -0.02313554190720879 1103 90-91 -0.0522896851131307 1103 92-93 -0.08838997607908539 1103 94-95 -0.19759940511851326 1103 96-97 -0.02139085840180499 1103 98-99 -0.044021003295515726 1103 100-101 -0.01499749731212674 1104 1 0.18756635734128224 1104 2 0.25102729886741315 1104 3 0.171674970801881 1104 4 0.09083857790108141 1104 5 0.029785866241866188 1104 6 0.02520664438420539 1104 7 0.08630165878464169 1104 8 0.11044039253249593 1104 9 0.0958312548028104 1104 10-11 0.12427193055925301 1104 12-13 0.14316434079256624 1104 14-15 0.16319315440453863 1104 16-17 0.15837900665457028 1104 18-19 0.14179548954442112 1104 20-21 0.18530664979201816 1104 22-23 0.0957759565755012 1104 24-25 0.11600370606640809 1104 26-27 0.1486401560938333 1104 28-29 0.0768662885708764 1104 30-31 0.11257914943887215 1104 32-33 0.2622521780378406 1104 34-35 0.10293667562442721 1104 36-37 0.177493025593229 1104 38-39 0.21225943576420292 1104 40-41 0.1769962594784218 1104 42-43 0.17538993414984816 1104 44-45 0.3656331669404338 1104 46-47 0.26642045367511 1104 48-49 0.25938050336755936 1104 50-51 0.2220413792595437 1104 52-53 0.29256370252536357 1104 54-55 0.2571014713188404 1104 56-57 0.347455774507722 1104 58-59 0.43241041198439945 1104 60-61 0.2504436464461435 1104 62-63 0.30330927153803344 1104 64-65 0.23487817002965272 1104 66-67 0.3365444742799184 1104 68-69 0.27044411606607355 1104 70-71 0.09904559322565021 1104 72-73 0.24241992810806323 1104 74-75 0.41520450282929744 1104 76-77 0.21855539393054713 1104 78-79 0.37319552105644505 1104 80-81 0.345940605589238 1104 82-83 0.15482696198201396 1104 84-85 0.26086154287814267 1104 86-87 0.31224428659115944 1104 88-89 0.40996715875974843 1104 90-91 0.4104935057606376 1104 92-93 0.41474118681340144 1104 94-95 0.485946260403896 1104 96-97 0.5720367467761065 1104 98-99 0.3674065102739803 1104 100-101 0.3300816676262812 1105 1 0.08884366133440835 1105 2 0.1996028056985537 1105 3 0.16201427432205406 1105 4 0.08961467493779196 1105 5 0.11806741903204454 1105 6 0.061953098674841556 1105 7 0.07691986884628221 1105 8 0.07827675045807325 1105 9 0.15291511873988384 1105 10-11 0.14180830871719508 1105 12-13 0.08765353044746149 1105 14-15 0.13489953463746218 1105 16-17 0.1903009340384827 1105 18-19 0.06511772957274076 1105 20-21 0.08137405462098002 1105 22-23 -0.016440848500906213 1105 24-25 -0.020078171890510532 1105 26-27 0.019959844567132734 1105 28-29 0.05048756401721732 1105 30-31 0.10908447375956598 1105 32-33 0.09766245525231909 1105 34-35 -0.1489084929536162 1105 36-37 -0.06395886406326667 1105 38-39 -0.062701147341933 1105 40-41 -0.014466613093368608 1105 42-43 0.021665471044940432 1105 44-45 -0.1038432545903234 1105 46-47 -0.18052630880984566 1105 48-49 -0.10061100007323631 1105 50-51 0.008142765150800813 1105 52-53 -0.0340003474045929 1105 54-55 0.053425377024630905 1105 56-57 0.07929871286833645 1105 58-59 -0.0033675406345992087 1105 60-61 -0.06015274525869074 1105 62-63 0.013756805284948825 1105 64-65 -0.17138950418473797 1105 66-67 -0.10822478618309361 1105 68-69 -0.08287846806771171 1105 70-71 -0.18046766052703944 1105 72-73 -0.11222112331267908 1105 74-75 0.033199045782197345 1105 76-77 -0.17975600548173176 1105 78-79 -0.19803676980116336 1105 80-81 -0.13637224372309475 1105 82-83 -0.1573100132118661 1105 84-85 -0.0599501889969396 1105 86-87 -0.0694311655453248 1105 88-89 -0.09831155321982976 1105 90-91 0.032480577743879024 1105 92-93 0.07720987450896288 1105 94-95 0.278070915069474 1105 96-97 0.42626823506591194 1105 98-99 0.47862954909269817 1105 100-101 0.3560747611965773 1106 1 0.1555941374433658 1106 2 0.23673769967069092 1106 3 0.18810665015925565 1106 4 0.10237840720407121 1106 5 0.1391527602256346 1106 6 0.049795067947087546 1106 7 0.13508941713121914 1106 8 0.1401413589788234 1106 9 0.17055094353178646 1106 10-11 0.13469496001285108 1106 12-13 0.1990984676438785 1106 14-15 0.2347928113370088 1106 16-17 0.27257316404725884 1106 18-19 0.2843729935189785 1106 20-21 0.1743041709199531 1106 22-23 0.14628349778543992 1106 24-25 0.20955706980165445 1106 26-27 0.17103289519516096 1106 28-29 0.25987346370266806 1106 30-31 0.26488230398660306 1106 32-33 0.2286909174738767 1106 34-35 0.27664023599594856 1106 36-37 0.3371533050677513 1106 38-39 0.5120771811411586 1106 40-41 0.38061441904665827 1106 42-43 0.23037565710190222 1106 44-45 0.35761004126057827 1106 46-47 0.37425243426250177 1106 48-49 0.37243354286216146 1106 50-51 0.41659570525736456 1106 52-53 0.3150184134282199 1106 54-55 0.30544522916337513 1106 56-57 0.4287141420083458 1106 58-59 0.35956242855206 1106 60-61 0.26540447608383033 1106 62-63 0.20667274530592294 1106 64-65 0.1963228190130053 1106 66-67 0.1281895249032985 1106 68-69 0.28750989508038316 1106 70-71 0.3432558057601689 1106 72-73 0.40841806633917344 1106 74-75 0.35529999693020997 1106 76-77 0.22225147584837757 1106 78-79 0.12331972614389031 1106 80-81 0.06890233473324159 1106 82-83 0.2466953202783877 1106 84-85 0.2852875846319698 1106 86-87 0.22689604167441502 1106 88-89 0.12376412135701997 1106 90-91 0.28444312294748286 1106 92-93 0.10582796825428531 1106 94-95 0.2970203860000815 1106 96-97 0.645134186320913 1106 98-99 0.6388712412588369 1106 100-101 0.4826193736759805 1107 1 0.1624427252539462 1107 2 0.24444103371188675 1107 3 0.10643856776383842 1107 4 0.06632245052536945 1107 5 0.1169545758977506 1107 6 0.13098666913629842 1107 7 0.10957710029112633 1107 8 0.13934102982188534 1107 9 0.11903788343410326 1107 10-11 0.0864157340625411 1107 12-13 0.1413354075461868 1107 14-15 0.22371561872711965 1107 16-17 0.1974291578888412 1107 18-19 0.224157183368213 1107 20-21 0.14337868249353392 1107 22-23 0.12177258782578804 1107 24-25 0.18475482941091315 1107 26-27 0.17872627489361292 1107 28-29 0.11705519018386923 1107 30-31 0.268810951257791 1107 32-33 0.17640208226470122 1107 34-35 0.09196859658206336 1107 36-37 0.21317262976514684 1107 38-39 0.2846742028018028 1107 40-41 0.22432924703561952 1107 42-43 0.23114479598488913 1107 44-45 0.17210953690720743 1107 46-47 0.34712884114294695 1107 48-49 0.2317952704051649 1107 50-51 0.18076405310191745 1107 52-53 0.12445911209035643 1107 54-55 0.2789653502081819 1107 56-57 0.378463292571098 1107 58-59 0.25570677979435885 1107 60-61 0.18183245973215634 1107 62-63 6.505258984645934E-5 1107 64-65 0.09177832973714573 1107 66-67 0.35798353637069624 1107 68-69 0.27127834379560767 1107 70-71 0.14588507949131824 1107 72-73 0.3458716127396819 1107 74-75 0.34976936409945836 1107 76-77 0.07283745482480342 1107 78-79 0.14399755052273377 1107 80-81 0.2785812739984799 1107 82-83 0.45354788982606564 1107 84-85 0.3211716629925583 1107 86-87 0.5522019718082021 1107 88-89 0.5438400398110481 1107 90-91 0.2881932556720521 1107 92-93 0.45917594553183605 1107 94-95 0.5987743411922644 1107 96-97 0.5510457386674865 1107 98-99 0.5998979985321427 1107 100-101 0.5590720440943286 1108 1 -0.016276149132799844 1108 2 0.18027896232960217 1108 3 0.003475105233590625 1108 4 -0.014242471916809052 1108 5 0.03538067446825721 1108 6 0.025685231704898115 1108 7 0.032183254666236394 1108 8 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20503.0 24 23701.0 25 28811.0 26 35210.0 27 44310.0 28 55032.0 29 67877.0 30 84156.0 31 103954.0 32 130176.0 33 166902.0 34 219381.0 35 299468.0 36 419774.0 37 508031.0 38 254122.0 39 9429.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.15885232200631 18.60229129094056 14.661129809989399 23.577726577063732 2 16.003593219901948 19.868200572655205 38.85739702387656 25.27080918356629 3 20.3424207195135 22.8117053845588 29.106784939949538 27.739088955978165 4 13.635420920306645 16.172950584409847 35.98119696812865 34.21043152715486 5 14.492374450104235 36.79154353020758 33.81901613684812 14.897065882840065 6 33.77406056359549 34.972638437223104 16.611898042775262 14.641402956406147 7 30.041403628504803 31.580996796147794 19.738649073916093 18.638950501431307 8 27.04620734840465 33.95338467651671 19.16523597339214 19.835172001686498 9 26.200702495131672 16.726838286591324 19.83350622721289 37.23895299106412 10-11 25.788438796584135 25.725423599001918 26.651693922066656 21.834443682347292 12-13 26.686348489208743 22.848279347660004 27.04504600829814 23.42032615483312 14-15 23.260891531424 24.68645383107664 25.320304589602728 26.732350047896634 16-17 23.11352912716336 27.626480094277095 26.086363036583943 23.173627741975604 18-19 23.100057556204508 26.601867277970687 27.78849952718981 22.509575638635003 20-21 23.854478916345588 25.60350050768812 27.663258318843305 22.87876225712299 22-23 23.347444878809494 25.47490680825354 27.801482385276472 23.376165927660495 24-25 23.524095803106864 25.629973789086506 27.860517598241636 22.98541280956499 26-27 23.18325508562697 25.72977953199246 27.980109632098337 23.106855750282232 28-29 22.91093276591808 26.390989502388095 27.744585030201442 22.953492701492383 30-31 23.2181744246557 25.687157160772223 27.599521127000877 23.495147287571196 32-33 23.191452856962755 25.63972286811969 27.7359948206027 23.432829454314852 34-35 23.787106629817707 25.73352574264446 27.490916356927357 22.988451270610476 36-37 23.651336568305613 25.505246403121834 27.705649371773845 23.137767656798715 38-39 23.267961564099394 25.82401644696181 27.792904631433508 23.11511735750529 40-41 23.545962903302552 25.80051970485981 27.476242754151393 23.177274637686242 42-43 23.372933962636154 25.82470873006003 27.142286237741793 23.660071069562026 44-45 23.54403885748086 25.686243283927308 27.244410066241237 23.525307792350596 46-47 23.937547465192825 26.167572652314213 26.87562157364342 23.01925830884954 48-49 23.897098133544855 26.013135634028284 26.797125660592265 23.292640571834596 50-51 23.452239084960826 26.227355400447177 27.161329608002312 23.15907590658969 52-53 23.908076135090667 26.29288551584138 26.565024046960627 23.234014302107326 54-55 23.471286424088074 26.13489635555798 26.696574291613373 23.69724292874057 56-57 23.707499179987522 26.21023413489158 26.568006051976468 23.514260633144428 58-59 24.114309330573043 25.891833070764637 26.531211550747802 23.46264604791452 60-61 23.66895259422413 26.073299957893635 27.15299359949131 23.104753848390924 62-63 23.301726421026668 26.18244399278773 27.18185611445599 23.33397347172961 64-65 23.303206109417296 26.41605978074378 26.828743472192574 23.451990637646354 66-67 23.48259034693047 26.237601332054055 26.461228077858358 23.81858024315712 68-69 23.507578910051215 26.0501039369004 26.800478119220788 23.6418390338276 70-71 23.65835596991496 26.004673676802696 26.625454437907514 23.711515915374825 72-73 23.597614231800044 25.94774112811172 26.896785628364718 23.557859011723526 74-75 23.41032500763572 26.563843419527682 26.415432739039684 23.61039883379691 76-77 23.63110436040097 26.154234937351607 26.49052975296294 23.72413094928449 78-79 23.614719874537947 26.197274741908505 26.575010763884084 23.61299461966946 80-81 23.752573567049243 26.15083472675638 26.465198548565063 23.631393157629326 82-83 23.726450220520878 26.16509036199397 26.600539472327206 23.507919945157944 84-85 23.555235881848546 26.34687457759246 26.455427983081137 23.642461557477855 86-87 23.514312455579567 26.38290797460644 26.36362791880992 23.73915165100407 88-89 23.477991995158874 26.424506825651285 26.065081420973048 24.03241975821679 90-91 23.562204508841724 26.118702484661526 26.334117043932853 23.9849759625639 92-93 23.636805876394533 26.10518429233038 26.590510842214083 23.667498989061006 94-95 23.37771775345841 26.064612801117136 26.692264629142663 23.865404816281792 96-97 23.232752173448993 25.894427413088106 26.91122181966672 23.96159859379618 98-99 23.103246669783324 26.24193299489922 26.358219956389544 24.296600378927906 100-101 23.043236258519702 25.940996793831538 26.45473827412892 24.56102867351984 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 87.0 1 71.5 2 73.0 3 101.5 4 92.0 5 87.0 6 159.0 7 310.5 8 478.5 9 617.5 10 685.0 11 716.5 12 767.5 13 856.0 14 1003.5 15 1185.5 16 1358.0 17 1531.5 18 1791.5 19 2036.5 20 2314.5 21 2711.5 22 3171.0 23 3912.5 24 4903.0 25 6248.0 26 8227.5 27 10782.0 28 13464.5 29 17049.5 30 21773.5 31 26776.5 32 32629.5 33 37391.0 34 41133.0 35 45841.0 36 52341.0 37 58201.5 38 61753.0 39 65454.0 40 69572.0 41 73680.0 42 77369.5 43 81976.5 44 86620.0 45 92022.5 46 97251.0 47 105417.5 48 119460.5 49 119282.0 50 106596.5 51 100476.5 52 100199.5 53 101064.0 54 100527.5 55 95241.0 56 86492.5 57 78946.0 58 74304.5 59 67562.5 60 57001.0 61 47532.0 62 39452.0 63 31039.5 64 22394.5 65 16626.0 66 13256.0 67 10650.0 68 8294.0 69 6541.5 70 5145.0 71 3657.0 72 2781.5 73 2407.5 74 1946.0 75 1477.5 76 1058.5 77 675.5 78 449.5 79 331.0 80 252.5 81 147.0 82 78.0 83 60.5 84 42.0 85 18.0 86 13.5 87 9.0 88 5.5 89 5.0 90 3.5 91 2.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013004941498616661 2 0.0102371259610102 3 0.006711004796662241 4 4.549833760448978E-4 5 0.005346054668527549 6 0.0 7 3.4123753203367334E-4 8 0.001630357097494217 9 1.1374584401122445E-4 10-11 0.0 12-13 0.0 14-15 3.222798913651359E-4 16-17 0.004815240729808501 18-19 0.0014028654094717682 20-21 4.928986573819725E-4 22-23 7.772632674100337E-4 24-25 3.033222506965985E-4 26-27 5.6872922005612226E-5 28-29 7.583056267414962E-5 30-31 6.256021420617344E-4 32-33 0.003279671835656971 34-35 9.85797314763945E-4 36-37 0.0012512042841234688 38-39 0.00504273241783095 40-41 1.8957640668537405E-5 42-43 0.001630357097494217 44-45 0.004891071292482651 46-47 0.0 48-49 0.002009509910864965 50-51 7.583056267414963E-4 52-53 5.876868607246596E-4 54-55 1.7061876601683667E-4 56-57 0.011526245526470745 58-59 0.0020474251922020397 60-61 0.0034313329610052706 62-63 3.222798913651359E-4 64-65 0.0033365447576625836 66-67 0.001080585518106632 68-69 0.0015734841754886048 70-71 0.0051185629805051 72-73 0.0026351120529266997 74-75 0.006919538844016154 76-77 0.0015545265348200674 78-79 0.006350809623960032 80-81 0.0026161544122581624 82-83 0.0025403238495840127 84-85 0.0018009758635110535 86-87 7.014327047358841E-4 88-89 0.002085340473539115 90-91 0.008284488972150847 92-93 0.002255959239555951 94-95 3.6019517270221077E-4 96-97 1.3270348467976184E-4 98-99 6.824750640673467E-4 100-101 0.001630357097494217 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2637459.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.907846254218796 #Duplication Level Percentage of deduplicated Percentage of total 1 76.03750288682508 41.7505551806451 2 13.639551922749835 14.978368399015649 3 4.4554411763072075 7.339160373101756 4 1.9353336181990513 4.25060003034778 5 1.0586461648442786 2.906399042844401 6 0.6218822359345632 2.048772851935489 7 0.4105977753297722 1.5781527664091983 8 0.2998180749337876 1.316989181016021 9 0.2211748405111831 1.0929810724280464 >10 1.1827116520969845 11.894689410469683 >50 0.09032063259588319 3.405596868733757 >100 0.04239001544440577 4.139725847152284 >500 0.0030399430750536823 1.14831121042666 >1k 0.0015199715375164148 1.830166040156246 >5k 6.908961534165521E-5 0.3195317253178854 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8423 0.31936041470218113 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4592 0.17410697189984753 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4401 0.16686515316446626 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4111 0.15586972157671455 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3475 0.13175560264633498 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3209 0.12167013781067308 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.791528133707481E-5 0.0 0.0 0.0 6 0.0 3.791528133707481E-5 0.0 0.0 0.0 7 0.0 3.791528133707481E-5 0.0 0.0 0.0 8 0.0 3.791528133707481E-5 0.0 0.0 0.0 9 0.0 3.791528133707481E-5 0.0 0.0 0.0 10-11 0.0 3.791528133707481E-5 0.0 0.0 0.0 12-13 0.0 3.791528133707481E-5 0.0 0.0 0.0 14-15 0.0 3.791528133707481E-5 0.0 0.0 1.8957640668537405E-5 16-17 0.0 3.791528133707481E-5 0.0 1.8957640668537405E-5 1.3270348467976184E-4 18-19 0.0 7.583056267414962E-5 0.0 7.583056267414962E-5 1.5166112534829924E-4 20-21 0.0 7.583056267414962E-5 0.0 7.583056267414962E-5 1.5166112534829924E-4 22-23 0.0 7.583056267414962E-5 0.0 1.3270348467976184E-4 1.7061876601683664E-4 24-25 0.0 7.583056267414962E-5 0.0 1.5166112534829924E-4 1.8957640668537407E-4 26-27 0.0 7.583056267414962E-5 0.0 2.4644932869098625E-4 1.8957640668537407E-4 28-29 0.0 7.583056267414962E-5 0.0 6.824750640673466E-4 1.8957640668537407E-4 30-31 0.0 7.583056267414962E-5 0.0 0.0010616278774380947 1.8957640668537407E-4 32-33 1.8957640668537405E-5 7.583056267414962E-5 0.0 0.0017630605821739786 2.2749168802244888E-4 34-35 3.791528133707481E-5 9.478820334268704E-5 0.0 0.0023128321615615635 2.2749168802244888E-4 36-37 3.791528133707481E-5 1.3270348467976184E-4 0.0 0.003090095428971597 2.2749168802244888E-4 38-39 3.791528133707481E-5 1.5166112534829924E-4 0.0 0.0043412997130950655 2.2749168802244888E-4 40-41 3.791528133707481E-5 1.5166112534829924E-4 0.0 0.005876868607246595 2.2749168802244888E-4 42-43 3.791528133707481E-5 1.5166112534829924E-4 0.0 0.008549895941510371 2.2749168802244888E-4 44-45 3.791528133707481E-5 1.7061876601683664E-4 0.0 0.013611586000009859 2.2749168802244888E-4 46-47 3.791528133707481E-5 1.8957640668537407E-4 0.0 0.027583367172721925 2.2749168802244888E-4 48-49 3.791528133707481E-5 1.8957640668537407E-4 0.0 0.03719489099167039 2.2749168802244888E-4 50-51 3.791528133707481E-5 1.8957640668537407E-4 0.0 0.04913820461284896 2.2749168802244888E-4 52-53 3.791528133707481E-5 2.0853404735391147E-4 0.0 0.06570718255715065 2.654069693595237E-4 54-55 3.791528133707481E-5 4.928986573819725E-4 0.0 0.08688286718390693 3.033222506965985E-4 56-57 3.791528133707481E-5 6.06644501393197E-4 0.0 0.10826708585801713 3.033222506965985E-4 58-59 3.791528133707481E-5 6.445597827302718E-4 0.0 0.13628647876611544 3.033222506965985E-4 60-61 3.791528133707481E-5 6.445597827302718E-4 0.0 0.17192684322296575 3.033222506965985E-4 62-63 3.791528133707481E-5 6.445597827302718E-4 0.0 0.21600335777731522 3.4123753203367334E-4 64-65 3.791528133707481E-5 6.635174233988093E-4 0.0 0.25016502626201964 3.601951727022107E-4 66-67 3.791528133707481E-5 7.203903454044214E-4 0.0 0.3107157305573281 3.981104540392856E-4 68-69 3.791528133707481E-5 7.203903454044214E-4 0.0 0.39060322833454475 4.1706809470782295E-4 70-71 3.791528133707481E-5 7.393479860729589E-4 0.0 0.4430779777050563 4.5498337604489775E-4 72-73 3.791528133707481E-5 7.583056267414963E-4 0.0 0.5312878797357609 5.687292200561222E-4 74-75 3.791528133707481E-5 7.583056267414963E-4 0.0 0.6011847008806582 5.687292200561222E-4 76-77 3.791528133707481E-5 7.583056267414963E-4 0.0 0.6610529301118995 5.687292200561222E-4 78-79 3.791528133707481E-5 7.583056267414963E-4 0.0 0.7386275957275544 5.687292200561222E-4 80-81 7.583056267414962E-5 7.583056267414963E-4 0.0 0.8265152178668939 5.876868607246595E-4 82-83 7.583056267414962E-5 7.583056267414963E-4 0.0 0.9366401525104276 6.445597827302718E-4 84-85 7.583056267414962E-5 7.583056267414963E-4 0.0 1.0992587941651415 6.445597827302718E-4 86-87 7.583056267414962E-5 7.583056267414963E-4 0.0 1.3258973883575063 6.445597827302718E-4 88-89 7.583056267414962E-5 7.583056267414963E-4 0.0 1.6038164005582645 6.445597827302718E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9875 0.0 22.183304 1 TACATGG 9800 0.0 21.57358 2 TAAGGTG 1260 0.0 21.110508 5 ACATGGG 9890 0.0 20.654325 3 GAGTACT 7270 0.0 20.417425 12-13 GTAAGGT 1180 0.0 19.724014 4 GTACTTT 7540 0.0 19.466732 14-15 CATGGGG 5770 0.0 18.768963 4 AGTACTT 7585 0.0 18.348366 12-13 AGAGTAC 12520 0.0 17.84993 10-11 GTATCAA 18115 0.0 17.73253 1 AGGTAAG 1315 0.0 17.703478 2 GGTAAGG 1350 0.0 17.594425 3 GTGTAGC 1725 0.0 16.80363 1 ACTTTTT 8915 0.0 16.650148 16-17 ATTGCGC 205 3.3367355E-4 16.217821 6 ATAGTAC 835 0.0 15.929804 3 TCAACGC 19960 0.0 15.801152 4 TACTTTT 8660 0.0 15.742363 14-15 CAACGCA 20100 0.0 15.7147255 5 >>END_MODULE