FastQCFastQC Report
Thu 26 May 2016
SRR936458_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936458_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2741481
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT76490.2790097761027707No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT46380.16917863009081588No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40750.14864228495473797No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT33820.12336397735384633No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31710.1156674075071102No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30620.11169145436353563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG103350.023.2698461
TACATGG101100.023.0817092
ACATGGG103500.021.582173
TTATTCG1856.5247223E-620.552464
GAGTACT58350.020.35756312-13
GTACTTT60250.019.35769814-15
GTATAGG7650.019.2598881
TAAGGTG12600.019.2369985
GTATAGC8750.019.0113721
CATGGGG59250.018.6901654
AGAGTAC110800.018.24928710-11
AGTACTT62450.018.06998612-13
GTACCGT2905.1863026E-818.0276956
GTGTAGC16600.017.7515831
AGGTAAG11800.017.7218192
GGTAAGG12200.017.5303383
TACCGTA2202.9082554E-517.2805427
GTATCAA173150.017.1558021
GTAAGGT13100.016.6890684
ACTTTTT74050.016.4242716-17
Produced by FastQC (version 0.11.5)