##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936458_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2741481 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.251651570811543 31.0 31.0 34.0 28.0 34.0 2 31.3066517696092 31.0 31.0 34.0 28.0 34.0 3 31.36556189884227 31.0 31.0 34.0 28.0 34.0 4 34.995684814157016 37.0 35.0 37.0 32.0 37.0 5 34.90051362748821 37.0 35.0 37.0 32.0 37.0 6 35.0152858983885 37.0 35.0 37.0 32.0 37.0 7 34.83672511317788 37.0 35.0 37.0 32.0 37.0 8 34.81922289448659 37.0 35.0 37.0 32.0 37.0 9 36.307877749289524 39.0 37.0 39.0 32.0 39.0 10-11 36.181219019938496 39.0 35.0 39.0 32.0 39.0 12-13 36.08375035245548 39.0 35.0 39.0 30.5 39.0 14-15 37.16341477471484 39.0 36.0 41.0 31.0 41.0 16-17 37.09149653052492 39.0 36.0 41.0 31.0 41.0 18-19 37.01849000594934 39.0 36.0 41.0 31.0 41.0 20-21 36.90066081070779 39.0 36.0 41.0 30.0 41.0 22-23 36.7998018224456 39.0 36.0 41.0 30.0 41.0 24-25 36.658229803525906 39.0 36.0 41.0 30.0 41.0 26-27 36.44932209998902 39.0 36.0 40.0 30.0 41.0 28-29 36.577512118449846 39.0 36.0 41.0 30.0 41.0 30-31 36.53629279210762 39.0 36.0 41.0 30.0 41.0 32-33 36.415370195890475 39.0 35.0 41.0 30.0 41.0 34-35 36.26544922251878 39.0 35.0 40.0 30.0 41.0 36-37 36.047586505250266 38.5 35.0 40.0 29.0 41.0 38-39 35.870470377142865 38.0 35.0 40.0 28.0 41.0 40-41 35.73742623056661 38.0 35.0 40.0 27.5 41.0 42-43 35.492564420472 38.0 34.5 40.0 27.0 41.0 44-45 35.26115282214248 38.0 34.0 40.0 26.5 41.0 46-47 34.99447652564435 38.0 34.0 40.0 25.5 41.0 48-49 34.79605421303303 38.0 33.5 40.0 24.5 41.0 50-51 34.11893990875734 37.5 33.0 39.5 24.0 40.5 52-53 34.31125585039619 37.5 33.5 39.5 24.5 40.5 54-55 34.66824501063476 38.0 34.0 40.0 25.0 41.0 56-57 34.573218089054784 38.0 34.0 40.0 25.0 41.0 58-59 34.33005919063456 38.0 33.5 40.0 24.0 41.0 60-61 33.98893536012105 37.0 33.0 40.0 23.0 41.0 62-63 33.6648920784058 37.0 33.0 40.0 23.0 41.0 64-65 33.342190224918575 36.0 32.5 39.0 22.0 41.0 66-67 32.9609599701767 36.0 32.0 39.0 22.0 41.0 68-69 32.575071284462666 35.0 31.5 39.0 21.0 40.0 70-71 32.176019640479 35.0 31.0 38.0 20.0 40.0 72-73 31.761544216429 35.0 31.0 37.0 19.5 39.5 74-75 31.33889127810844 35.0 31.0 37.0 18.0 39.0 76-77 30.917340846060945 35.0 30.0 36.0 17.0 39.0 78-79 30.475208290701268 34.0 30.0 36.0 12.5 37.5 80-81 30.0719202504048 34.0 30.0 35.0 9.0 37.0 82-83 29.677468310011996 34.0 29.0 35.0 7.0 37.0 84-85 29.303307409389305 34.0 29.0 35.0 4.5 36.0 86-87 28.970124177406298 34.0 29.0 35.0 2.0 36.0 88-89 28.599331346815827 34.0 29.0 35.0 2.0 35.5 90-91 28.251870613000783 34.0 27.5 35.0 2.0 35.0 92-93 27.890239618658676 34.0 27.0 35.0 2.0 35.0 94-95 27.462287172517335 33.0 26.5 35.0 2.0 35.0 96-97 26.880545953081565 33.0 25.5 35.0 2.0 35.0 98-99 26.212426057302604 33.0 24.5 35.0 2.0 35.0 100-101 24.106607158685396 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.431611997343051 1101 2 0.5931304799056427 1101 3 0.4817239911276374 1101 4 0.43520521051277683 1101 5 0.49841476449680044 1101 6 0.36629379747892443 1101 7 0.44702783092873233 1101 8 0.5177223761932268 1101 9 0.7360708779682383 1101 10-11 0.6353465446415711 1101 12-13 0.7200266298693663 1101 14-15 0.8209615583032601 1101 16-17 0.8703702738189207 1101 18-19 0.8920576758096175 1101 20-21 0.906162233445805 1101 22-23 0.8336472034239009 1101 24-25 0.7524434712600794 1101 26-27 0.9683625634338213 1101 28-29 0.7969201969459832 1101 30-31 0.6352792463659185 1101 32-33 0.5459235748910984 1101 34-35 0.6457941692133886 1101 36-37 0.6770969819033823 1101 38-39 0.6670844171902317 1101 40-41 0.5936553453973374 1101 42-43 0.4815000660433526 1101 44-45 0.4850864115952831 1101 46-47 0.6442465569035747 1101 48-49 0.4122574349167465 1101 50-51 0.6962644581318784 1101 52-53 0.5895448321004153 1101 54-55 0.6779479168085629 1101 56-57 0.604766544825992 1101 58-59 0.5699754613157069 1101 60-61 0.5732453892824836 1101 62-63 0.6409236607277293 1101 64-65 0.7456243566466867 1101 66-67 0.5934488519849239 1101 68-69 0.6627386682462557 1101 70-71 0.5552537723238942 1101 72-73 0.6057024643630022 1101 74-75 0.4271067436872791 1101 76-77 0.468469067049341 1101 78-79 0.5281646237005013 1101 80-81 0.593258877770797 1101 82-83 0.6648583751280945 1101 84-85 0.5425168196846961 1101 86-87 0.5031849539955324 1101 88-89 0.46295230611054805 1101 90-91 0.4890790390409272 1101 92-93 0.30102849140416765 1101 94-95 0.18176526418221073 1101 96-97 0.24636381258536133 1101 98-99 0.008618408364139185 1101 100-101 0.15960119470019762 1102 1 0.2101929799188227 1102 2 0.2607753158956072 1102 3 0.2787684405375863 1102 4 0.27224092515193377 1102 5 0.34702808707965005 1102 6 0.38202341647728844 1102 7 0.06492457937514473 1102 8 0.1990068927312123 1102 9 0.48874300425729444 1102 10-11 0.3635980331204749 1102 12-13 0.5420530055436856 1102 14-15 0.4146001763031322 1102 16-17 0.46255420350740195 1102 18-19 0.3102119098816871 1102 20-21 0.1624713083882554 1102 22-23 -0.02577051498313665 1102 24-25 -0.0036116208196119715 1102 26-27 0.2982743875633531 1102 28-29 0.31467842349061925 1102 30-31 0.19946711581625465 1102 32-33 0.2717112798548058 1102 34-35 0.15415939201254503 1102 36-37 0.09592513974178019 1102 38-39 -0.06018200061098611 1102 40-41 -0.2471309298140909 1102 42-43 -0.06573260804135828 1102 44-45 0.04324412499166641 1102 46-47 0.08732932940420568 1102 48-49 0.13972164813890942 1102 50-51 0.19838623396233857 1102 52-53 0.18526440376850672 1102 54-55 0.0596854616868967 1102 56-57 0.11897220154064314 1102 58-59 0.06657667449567839 1102 60-61 -0.18686405317662746 1102 62-63 -0.06900661996728275 1102 64-65 0.232874885707119 1102 66-67 0.007109848603001012 1102 68-69 -0.06249158958313927 1102 70-71 0.12756030392193907 1102 72-73 -0.15889604869461849 1102 74-75 -0.23011060157784513 1102 76-77 -0.08556039939002247 1102 78-79 -0.13722822222532827 1102 80-81 0.006570900242650168 1102 82-83 -0.11718064174402443 1102 84-85 -0.24600790773785164 1102 86-87 -0.2593156124363851 1102 88-89 -0.4887332650585634 1102 90-91 -0.38567954376985725 1102 92-93 -0.367872260869504 1102 94-95 -0.3945010822616126 1102 96-97 -0.6661092395789829 1102 98-99 -0.640192175761289 1102 100-101 -0.17503447629114177 1103 1 0.28759068404939825 1103 2 0.34242212453261445 1103 3 0.4379101763848219 1103 4 0.3787831265609398 1103 5 0.4572308791441273 1103 6 0.2750443889372747 1103 7 0.38074950241919936 1103 8 0.08999998151337962 1103 9 0.4179710098767657 1103 10-11 0.5158927504077155 1103 12-13 0.5904233976287188 1103 14-15 0.5959297222491742 1103 16-17 0.7450222452706896 1103 18-19 0.6149814351163698 1103 20-21 0.4423179620844522 1103 22-23 0.4168635552124229 1103 24-25 0.5358462243497399 1103 26-27 0.49555443328407733 1103 28-29 0.5890781818746404 1103 30-31 0.4080185695600633 1103 32-33 0.5527737149393062 1103 34-35 0.4815409231023011 1103 36-37 0.5500938842591196 1103 38-39 0.4710173046074999 1103 40-41 0.2978891475528087 1103 42-43 0.46363073358366336 1103 44-45 0.587685607972638 1103 46-47 0.601970090124901 1103 48-49 0.6354767332201945 1103 50-51 0.17803112204305194 1103 52-53 0.507220295542588 1103 54-55 0.6310658088484615 1103 56-57 0.5577968274936396 1103 58-59 0.5845612807181766 1103 60-61 0.7812051888789071 1103 62-63 0.5180368089293168 1103 64-65 0.5802562147108787 1103 66-67 0.49863782451335936 1103 68-69 0.38440914442031016 1103 70-71 0.5220706132034678 1103 72-73 0.519460799932002 1103 74-75 0.47330737498190345 1103 76-77 0.29766889046130274 1103 78-79 0.43297146215084936 1103 80-81 0.5986699292810549 1103 82-83 0.601960487845222 1103 84-85 0.5345361890899305 1103 86-87 0.37438053919317227 1103 88-89 0.2779362727079189 1103 90-91 0.5013221001504284 1103 92-93 0.4839374623503119 1103 94-95 0.39086880349942277 1103 96-97 0.5941140013702473 1103 98-99 0.427580689930263 1103 100-101 0.6507556275822921 1104 1 0.298574158371121 1104 2 0.29458672845318645 1104 3 0.29428939883758076 1104 4 0.39035189694878625 1104 5 0.18820629302824443 1104 6 0.30227342114209677 1104 7 0.4299777820156905 1104 8 0.446870988031435 1104 9 0.5953152429293311 1104 10-11 0.6274845692772857 1104 12-13 0.5173538920287442 1104 14-15 0.5080939889328135 1104 16-17 0.2296429350650797 1104 18-19 0.47532918813877245 1104 20-21 0.4523610322277207 1104 22-23 0.5391067175258684 1104 24-25 0.37600500357104494 1104 26-27 0.3850977016727839 1104 28-29 0.40841370875212135 1104 30-31 0.3053362178929575 1104 32-33 0.17063992188712263 1104 34-35 0.24969712412353573 1104 36-37 0.3609984285148755 1104 38-39 0.26581385752359665 1104 40-41 0.1904632734768441 1104 42-43 0.22074515629281422 1104 44-45 0.054949876686826826 1104 46-47 0.20461094027545101 1104 48-49 0.014119292829491314 1104 50-51 -0.08808829854115174 1104 52-53 0.06482517236857888 1104 54-55 0.2675682747747601 1104 56-57 0.3574426455954054 1104 58-59 0.2547326980121909 1104 60-61 0.2581023217593952 1104 62-63 0.00643229617670471 1104 64-65 0.08672314845178164 1104 66-67 0.10527475416585474 1104 68-69 0.19372630019729087 1104 70-71 0.08431487272437721 1104 72-73 -0.18857632795406332 1104 74-75 -0.33829954830227393 1104 76-77 -0.2033292239805249 1104 78-79 -0.08667318274638447 1104 80-81 -0.08353009995406424 1104 82-83 -0.22302565753548365 1104 84-85 -0.1331771984680934 1104 86-87 -0.08802407411303292 1104 88-89 -0.20656816534195244 1104 90-91 -0.1590316851381779 1104 92-93 -0.3393980032545514 1104 94-95 -0.2533842932914965 1104 96-97 -0.27088958058468293 1104 98-99 -0.34123692295712615 1104 100-101 -0.11204194986909854 1105 1 0.11734598148816033 1105 2 0.2669949153117699 1105 3 0.22101764161147486 1105 4 0.34857392243023355 1105 5 0.4406193531546734 1105 6 0.3838131886622307 1105 7 0.46336302205363467 1105 8 0.10335859527196334 1105 9 0.3747917738984796 1105 10-11 0.4718834524983109 1105 12-13 0.27451694206038013 1105 14-15 0.36296224120101783 1105 16-17 0.34020201759489055 1105 18-19 0.255618335364332 1105 20-21 -0.18402394019701518 1105 22-23 -0.13637830394393546 1105 24-25 -0.19705421345955898 1105 26-27 -0.1257570049471184 1105 28-29 0.015111837229838443 1105 30-31 -0.041107945298570314 1105 32-33 -0.18665709673044972 1105 34-35 -0.34776400977204247 1105 36-37 -0.3241321228828795 1105 38-39 -0.32405483613865016 1105 40-41 -0.44424671559995943 1105 42-43 -0.33082071653178247 1105 44-45 -0.19431742656684747 1105 46-47 -0.21471732857713732 1105 48-49 0.0010074602412828426 1105 50-51 -0.08883294127288366 1105 52-53 0.07354429546730046 1105 54-55 0.055517430809693735 1105 56-57 -0.009690170969449241 1105 58-59 -0.16702335374303345 1105 60-61 -0.10852054874577988 1105 62-63 -0.3328983653439437 1105 64-65 -0.24475596354440654 1105 66-67 -0.36832635358855015 1105 68-69 -0.014261809584517948 1105 70-71 0.014128824270073892 1105 72-73 -0.08605350938644918 1105 74-75 -0.027769934464785706 1105 76-77 -0.1732195557850389 1105 78-79 -0.11686609681592941 1105 80-81 -0.1580326118505866 1105 82-83 -0.09176016174122026 1105 84-85 -0.27069180409842986 1105 86-87 -0.07438089103608903 1105 88-89 -0.026785944416079843 1105 90-91 -0.03433040708550905 1105 92-93 -0.09539526395042941 1105 94-95 -0.034823147606125815 1105 96-97 -0.04679469579199491 1105 98-99 -0.4780860243934093 1105 100-101 0.011301014685979993 1106 1 0.034216716929247326 1106 2 0.07639213452566906 1106 3 0.08149672485396309 1106 4 0.17173761638184715 1106 5 0.38284361226875063 1106 6 0.358453808478572 1106 7 0.37935608472966464 1106 8 0.3265387333868617 1106 9 0.53039340577498 1106 10-11 0.4412040065425842 1106 12-13 0.37643366316770965 1106 14-15 0.4031739620999275 1106 16-17 0.30164246428818586 1106 18-19 0.3944300975803543 1106 20-21 0.4010836766314725 1106 22-23 0.3564272597871323 1106 24-25 0.21091307551686356 1106 26-27 0.20586951916671836 1106 28-29 0.1448237876235794 1106 30-31 0.20728963874149287 1106 32-33 0.1051432026865271 1106 34-35 0.14894486090092585 1106 36-37 0.23574937808797358 1106 38-39 0.12129427333297116 1106 40-41 0.07252012993526336 1106 42-43 0.07990774497319819 1106 44-45 0.006983706369403819 1106 46-47 0.09469975683960996 1106 48-49 -0.04316659204036455 1106 50-51 0.13151804285631385 1106 52-53 0.022562681121279127 1106 54-55 0.21892154256248375 1106 56-57 0.12471841389431404 1106 58-59 0.1257803037512275 1106 60-61 0.1700978360814034 1106 62-63 0.16974125587990585 1106 64-65 0.029421808195678523 1106 66-67 0.10235922677500042 1106 68-69 -0.021778181689754206 1106 70-71 0.051745766443460184 1106 72-73 -0.0016223769566181545 1106 74-75 0.13372719406507017 1106 76-77 -0.11730670204174487 1106 78-79 0.19060128730008685 1106 80-81 0.07634000714768163 1106 82-83 0.19375816873524698 1106 84-85 0.22398342195426935 1106 86-87 0.36879608456304425 1106 88-89 0.44212396488034855 1106 90-91 0.4104963820182128 1106 92-93 0.30369682761278227 1106 94-95 0.29638216707395415 1106 96-97 0.3533381895833436 1106 98-99 0.5608989297713549 1106 100-101 0.9518309622977661 1107 1 0.45923982431997956 1107 2 0.5817125493025088 1107 3 0.5439275761110771 1107 4 0.48442993296797 1107 5 0.4730542050005724 1107 6 0.42539712517370276 1107 7 0.6609714548608423 1107 8 0.6103400395573075 1107 9 0.6966286684130196 1107 10-11 0.7945573365504899 1107 12-13 0.6844020296618325 1107 14-15 0.9299316929178261 1107 16-17 0.9149480929776885 1107 18-19 1.1232153326348708 1107 20-21 1.1023666571049873 1107 22-23 0.8656921507579085 1107 24-25 1.0206505530949315 1107 26-27 0.9440219189328332 1107 28-29 0.7863527404078638 1107 30-31 0.8240006922997694 1107 32-33 0.934527677294831 1107 34-35 0.7807638205232763 1107 36-37 1.00957000923853 1107 38-39 1.0751011622754518 1107 40-41 0.9305715773267238 1107 42-43 0.9720078913144974 1107 44-45 0.8974321450101144 1107 46-47 1.1228264229997578 1107 48-49 1.1299219361889712 1107 50-51 0.9932589427727336 1107 52-53 0.9980755859135471 1107 54-55 1.1243675531928758 1107 56-57 1.1633909033811989 1107 58-59 1.0952967167706547 1107 60-61 1.0539302604249414 1107 62-63 1.0770052681421873 1107 64-65 1.0046888067454773 1107 66-67 0.9077550254912907 1107 68-69 1.0741983904882204 1107 70-71 1.0988505540834055 1107 72-73 1.1189873013898257 1107 74-75 0.8712323886857867 1107 76-77 1.0899716470433702 1107 78-79 1.203245506157966 1107 80-81 1.1232391902130523 1107 82-83 1.1546749325773824 1107 84-85 1.2352303621657548 1107 86-87 1.2914587034447074 1107 88-89 1.3003495683317574 1107 90-91 1.3590388821090578 1107 92-93 1.3965057547625648 1107 94-95 1.4948211711602788 1107 96-97 1.6033725120693596 1107 98-99 1.884305411733056 1107 100-101 2.0838964227773538 1108 1 0.16427484410758453 1108 2 0.09958488367030327 1108 3 0.09696134315831273 1108 4 0.11472361517051155 1108 5 0.3017862324469789 1108 6 0.2927996819029133 1108 7 0.31180737434380035 1108 8 0.16776338038810223 1108 9 0.32410752841929025 1108 10-11 0.1988397156183268 1108 12-13 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0.1919656651076842 2311 2 0.2321410337695582 2311 3 0.19989241417841086 2311 4 0.19883566397781038 2311 5 0.3551841431989402 2311 6 0.329344382274698 2311 7 0.518069976367606 2311 8 0.39485847171479804 2311 9 0.46446953177878925 2311 10-11 0.4210308602067201 2311 12-13 0.395939985908889 2311 14-15 0.46181562827543843 2311 16-17 0.5344440502496113 2311 18-19 0.5835420524708965 2311 20-21 0.5970361026098345 2311 22-23 0.5878479514106587 2311 24-25 0.7020811732779677 2311 26-27 0.607196260076492 2311 28-29 0.6408539423929511 2311 30-31 0.3387136087385869 2311 32-33 0.3059652789648837 2311 34-35 0.5150567472107923 2311 36-37 0.6310698252436708 2311 38-39 0.5758941629048167 2311 40-41 0.638981621038397 2311 42-43 0.6033263606148722 2311 44-45 0.7769803086726839 2311 46-47 0.70562087495302 2311 48-49 0.11111704295757363 2311 50-51 0.689795231029052 2311 52-53 0.7598576878570142 2311 54-55 0.72682242286605 2311 56-57 0.6415342405319961 2311 58-59 0.6822946429594836 2311 60-61 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-0.8750517486058484 2314 58-59 -0.9345765398416006 2314 60-61 -1.0338537209413161 2314 62-63 -1.0066491923284886 2314 64-65 -1.007232219239583 2314 66-67 -0.8667417471449212 2314 68-69 -0.9002382550597758 2314 70-71 -0.8578242737330513 2314 72-73 -0.7995285815034237 2314 74-75 -0.7510070475365502 2314 76-77 -0.6877623329121789 2314 78-79 -0.7632261497821737 2314 80-81 -0.7976304894957806 2314 82-83 -0.6974880438075317 2314 84-85 -0.5820410342059326 2314 86-87 -0.5966687985851316 2314 88-89 -0.5139901465818291 2314 90-91 -0.536703066723355 2314 92-93 -0.5078891027726016 2314 94-95 -0.6735496606186757 2314 96-97 -0.5297181393910222 2314 98-99 -0.5360895837370379 2314 100-101 -0.8461974670189072 2315 1 -0.7998174906666158 2315 2 -0.943141557408385 2315 3 -0.9948563733326843 2315 4 -0.93321374783023 2315 5 -0.851972967665489 2315 6 -0.8766883195861368 2315 7 -1.3123641947592617 2315 8 -0.7801952193532955 2315 9 -1.5993204082407715 2315 10-11 -0.9149704031688088 2315 12-13 -1.3354237899431283 2315 14-15 -1.2793376934191443 2315 16-17 -1.398851360291232 2315 18-19 -1.276532410624398 2315 20-21 -1.1741610760587733 2315 22-23 -1.0244064443923122 2315 24-25 -1.1935231553020458 2315 26-27 -1.2124150857150369 2315 28-29 -1.4911026656087927 2315 30-31 -1.4364483614096812 2315 32-33 -1.1317167259226295 2315 34-35 -1.096268295011356 2315 36-37 -1.2761142896149096 2315 38-39 -1.3351765299162608 2315 40-41 -1.4875271882672365 2315 42-43 -1.5081765157051592 2315 44-45 -1.6564822922305353 2315 46-47 -1.8893336236756113 2315 48-49 -1.8891684842170733 2315 50-51 -1.9117480388652268 2315 52-53 -2.1128561513670903 2315 54-55 -1.9201008917701898 2315 56-57 -2.0437672749685163 2315 58-59 -2.051166247770489 2315 60-61 -2.2101782204624207 2315 62-63 -2.1481096830518993 2315 64-65 -2.0080590741799043 2315 66-67 -1.8983406327821086 2315 68-69 -1.9379421862671862 2315 70-71 -1.7957328092960978 2315 72-73 -1.3824165650609466 2315 74-75 -1.2931995419829434 2315 76-77 -1.569322584747006 2315 78-79 -1.5183312295821203 2315 80-81 -1.4288262601845396 2315 82-83 -1.6003391290314752 2315 84-85 -1.4820235269015924 2315 86-87 -1.5382918586918741 2315 88-89 -1.629727000242351 2315 90-91 -1.441074921867525 2315 92-93 -1.4349261037953518 2315 94-95 -1.4398083431406086 2315 96-97 -1.3544135548101401 2315 98-99 -1.1714579467953605 2315 100-101 -1.2837316558627627 2316 1 -3.3759912216695014 2316 2 -2.797103863545434 2316 3 -2.8184244926602915 2316 4 -2.248114800007407 2316 5 -5.732609545840528 2316 6 -6.050676946865956 2316 7 -5.102422758346883 2316 8 -3.9488563461006727 2316 9 -7.723948774906898 2316 10-11 -6.99089988046109 2316 12-13 -6.236621850812206 2316 14-15 -5.2142919513606145 2316 16-17 -4.805050533792063 2316 18-19 -4.898065822526405 2316 20-21 -4.847438550184123 2316 22-23 -4.544374244924654 2316 24-25 -3.8680357461868695 2316 26-27 -4.327124316356432 2316 28-29 -3.796236553889109 2316 30-31 -3.4377742077977373 2316 32-33 -2.6691514125281657 2316 34-35 -2.74200323842949 2316 36-37 -2.3625370065948204 2316 38-39 -2.628945815931381 2316 40-41 -2.293278754308382 2316 42-43 -2.0782618111065077 2316 44-45 -2.310252657450711 2316 46-47 -2.349730737709386 2316 48-49 -2.6845140781475507 2316 50-51 -2.829129997230833 2316 52-53 -2.992561318276177 2316 54-55 -2.727190719832766 2316 56-57 -2.56393754582988 2316 58-59 -2.666393592343475 2316 60-61 -3.050123515271636 2316 62-63 -2.6702567853863286 2316 64-65 -2.54532698540239 2316 66-67 -3.359065328527464 2316 68-69 -2.8266989468183255 2316 70-71 -2.5632655937310993 2316 72-73 -2.4964831672936576 2316 74-75 -2.5438014614398625 2316 76-77 -3.256571108480447 2316 78-79 -3.3171357712208156 2316 80-81 -3.175980334374639 2316 82-83 -3.1953569263644077 2316 84-85 -3.19615714967836 2316 86-87 -3.364189461233064 2316 88-89 -3.5458948529203553 2316 90-91 -3.6513491927594544 2316 92-93 -3.718696865360755 2316 94-95 -3.8408451201567857 2316 96-97 -4.062902600878825 2316 98-99 -4.8206003846718275 2316 100-101 -5.073640276801061 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6353.0 3 2588.0 4 2839.0 5 3049.0 6 3400.0 7 3975.0 8 5027.0 9 6286.0 10 7646.0 11 9256.0 12 10853.0 13 12113.0 14 13646.0 15 15511.0 16 17341.0 17 19949.0 18 18600.0 19 16684.0 20 17305.0 21 19528.0 22 21844.0 23 25234.0 24 28968.0 25 33821.0 26 39561.0 27 46893.0 28 56037.0 29 67009.0 30 81136.0 31 98788.0 32 123499.0 33 155113.0 34 203220.0 35 275142.0 36 380478.0 37 482385.0 38 359969.0 39 50420.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.562547106691504 17.853445200097187 14.070499701216802 23.513507991994505 2 15.88137766501721 19.354285641327763 39.618283992478034 25.146052701176995 3 20.30270696193181 22.852369252881424 28.671069743348415 28.173854041838347 4 13.222304565760682 15.692793164586247 36.33269368094226 34.75220858871081 5 14.643711994674922 36.684875527371496 33.69864183478752 14.972770643166058 6 33.6831309514685 34.65719176457277 17.014785373028648 14.644891910930081 7 30.137303678579798 31.420804105099776 19.862915236724064 18.578976979596366 8 26.96606502409854 33.941908108127826 19.29309734642426 19.798929521349372 9 26.332874394496237 17.021413983990406 19.78030036051141 36.865411261001945 10-11 25.80870828186325 25.694175563312307 26.712890906536412 21.78422524828803 12-13 26.711688542958502 22.8631774925407 26.98758179319848 23.437552171302322 14-15 23.43673270440481 24.75322496732894 25.216256505936975 26.593785822329274 16-17 23.295832664438638 27.675986194013515 25.86798207848689 23.16019906306096 18-19 23.13954809979963 26.836215139245983 27.447908295467006 22.57632846548738 20-21 24.03667622696471 25.830226327681675 27.205803749130286 22.927293696223323 22-23 23.528002921466616 25.67319047283762 27.3284085266841 23.470398079011662 24-25 23.610350901159617 25.786149297918627 27.4307380084771 23.172761792444657 26-27 23.26145102734823 25.902599125812426 27.581301906597076 23.25464794024227 28-29 23.05561478034251 26.58839510716728 27.26450000610993 23.091490106380284 30-31 23.35443595552672 25.84748073577862 27.172643209675456 23.6254400990192 32-33 23.213435992182948 25.95412816427848 27.265317437808744 23.567118405729833 34-35 23.941908229345295 25.967135292244425 26.944912875475897 23.14604360293439 36-37 23.7655625329335 25.72531220822673 27.24979704116941 23.259328217670358 38-39 23.38600432527987 26.001709756831136 27.281254998722332 23.331030919166658 40-41 23.62896456780662 25.948619981855835 27.127698962966424 23.29471648737113 42-43 23.443962401765337 25.992350422229627 26.769382064328166 23.79430511167687 44-45 23.55788758347123 25.929726309745778 26.88389690303102 23.628489203751972 46-47 24.021273743505844 26.205753796179515 26.60890436755572 23.16406809275892 48-49 23.85266218121399 26.167691973787644 26.554749211641266 23.424896633357104 50-51 23.441256602866467 26.430342927742274 26.854027999327357 23.274372470063906 52-53 23.815499961334357 26.40152007038606 26.35793003123892 23.42504993704066 54-55 23.41272743555001 26.247584043020222 26.542797251850647 23.796891269579124 56-57 23.70146681790268 26.33294065226642 26.317911892436392 23.647680637394508 58-59 24.05158294454314 26.0228495555207 26.310092251797435 23.615475248138733 60-61 23.60353935816988 26.227453827024494 26.913512246679 23.255494568126625 62-63 23.362586783675603 26.306766181168094 26.873438550427643 23.45720848472866 64-65 23.289078640159936 26.617856459543077 26.470229354593776 23.62283554570321 66-67 23.426264897454153 26.38458047835273 26.251346023649162 23.937808600543953 68-69 23.475932727201094 26.195374776202378 26.62602560230196 23.702666894294566 70-71 23.662692858125222 26.115323987787825 26.401986867790335 23.819996286296615 72-73 23.54165553324514 26.071430330194335 26.715250638321237 23.671663498239294 74-75 23.364493481465782 26.76236801561844 26.243011947834216 23.630126555081567 76-77 23.62807725081418 26.28074697381783 26.27832121304089 23.812854562327097 78-79 23.54215497158337 26.422368708882725 26.365263602098914 23.670212717434993 80-81 23.795899372955372 26.35105195478741 26.25257969767667 23.60046897458055 82-83 23.684001257761608 26.389666073047934 26.437196913377143 23.48913575581332 84-85 23.565992542044935 26.523675103986015 26.262168789676476 23.648163564292577 86-87 23.59093216414354 26.573872744819326 26.23311846345756 23.602076627579574 88-89 23.56426495012824 26.519179916617148 25.92100333742988 23.995551795824728 90-91 23.619222382030753 26.312749042136858 26.170307656247292 23.897720919585097 92-93 23.717892592495307 26.299290215066 26.430295442141727 23.552521750296957 94-95 23.48215842591127 26.263016662933637 26.517932771895314 23.73689213925978 96-97 23.240112746011775 26.25010806589229 26.811558253626 23.698220934469937 98-99 23.21841659529406 26.5148733216036 26.191019547429264 24.07569053567308 100-101 23.220880846391402 26.140906375632344 26.44765137186828 24.190561406107967 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 97.0 1 66.0 2 58.0 3 89.5 4 87.0 5 93.0 6 156.5 7 290.0 8 484.0 9 644.0 10 667.0 11 673.0 12 760.0 13 865.0 14 971.0 15 1090.5 16 1213.0 17 1332.5 18 1518.5 19 1734.5 20 1994.0 21 2414.5 22 2893.0 23 3586.0 24 4615.0 25 5961.0 26 7819.5 27 10437.0 28 13381.0 29 17126.5 30 21828.0 31 27018.5 32 33492.5 33 38712.0 34 42815.5 35 47557.5 36 54228.0 37 60664.0 38 64480.0 39 68047.5 40 72299.5 41 77287.0 42 81238.5 43 85484.0 44 90533.5 45 96924.0 46 102524.0 47 110178.0 48 123180.0 49 122830.5 50 110832.0 51 104746.5 52 104063.0 53 104956.0 54 105023.0 55 99153.5 56 89968.0 57 82678.5 58 77272.5 59 70012.5 60 59486.0 61 50074.5 62 41679.0 63 32788.0 64 24015.0 65 17992.5 66 13974.0 67 11135.0 68 8768.0 69 6838.5 70 5417.0 71 3937.0 72 3012.5 73 2597.0 74 1971.0 75 1407.0 76 1072.5 77 698.0 78 477.5 79 322.5 80 228.5 81 170.5 82 103.5 83 57.0 84 38.0 85 28.0 86 18.5 87 14.5 88 14.0 89 11.0 90 8.0 91 7.0 92 7.5 93 6.0 94 4.0 95 2.5 96 2.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013241018267133713 2 0.0064198876446708904 3 0.007878953018459731 4 0.03392326994059051 5 0.04523102658745401 6 0.05876385792934549 7 0.025351260869581078 8 0.023746288958413353 9 0.013678737879270365 10-11 0.0443555873631807 12-13 0.04047082580546792 14-15 0.019077279762289068 16-17 0.023381522614966146 18-19 0.029527835502051628 20-21 0.023053232905863655 22-23 0.014298840663130622 24-25 0.003519995214265574 26-27 0.0028634157960605966 28-29 0.0015502569596506414 30-31 0.00709470538004823 32-33 0.0014043504222717574 34-35 0.0021885980606832583 36-37 0.005890976446672438 38-39 0.005672116640604112 40-41 0.0037935699718509813 42-43 8.207242727562219E-4 44-45 0.0015502569596506414 46-47 0.0026810326243369917 48-49 9.483924929627453E-4 50-51 0.0021156447919938167 52-53 0.0010578223959969084 54-55 0.0026445559899922707 56-57 0.0025716027213028288 58-59 0.002589841038475189 60-61 8.572009071009428E-4 62-63 0.001386112105099397 64-65 0.004176574632470552 66-67 0.002662794307164631 68-69 0.00601864466687896 70-71 0.010286410885211315 72-73 0.0040306680950916675 74-75 0.0038482849233680626 76-77 0.002662794307164631 78-79 0.03357674191431565 80-81 0.022159555364417993 82-83 0.0037570933375062603 84-85 0.009009728683146081 86-87 0.007641854895219044 88-89 0.005471495151708146 90-91 0.012894490240858864 92-93 0.01345987807320204 94-95 0.006821130622462822 96-97 0.0035747101657826554 98-99 0.02128411614014469 100-101 0.03990543797312474 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2741481.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.02240675015583 #Duplication Level Percentage of deduplicated Percentage of total 1 77.59996811632462 44.249369457281865 2 12.80977961839485 14.60888927559934 3 4.1534036190402235 7.105112116874428 4 1.8188242709891975 4.148549495496067 5 0.9505767486216001 2.710208700357076 6 0.5799463777988257 1.9841962948874512 7 0.3842318187327704 1.5336876137892526 8 0.27131445491302547 1.2376802564197882 9 0.21383483071662082 1.0974039025026483 >10 1.094573879831786 11.33031216222333 >50 0.08010317256652791 3.123325985351289 >100 0.039336975531934444 3.9610062455831403 >500 0.0026304809071722515 1.0211824829207616 >1k 0.0014114775599097366 1.6092413526078004 >5k 6.415807090498804E-5 0.2798346581057373 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7649 0.2790097761027707 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4638 0.16917863009081588 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4075 0.14864228495473797 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3382 0.12336397735384633 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3171 0.1156674075071102 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3062 0.11169145436353563 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.647663434472097E-5 2 0.0 0.0 0.0 0.0 3.647663434472097E-5 3 0.0 0.0 0.0 0.0 3.647663434472097E-5 4 0.0 0.0 0.0 0.0 3.647663434472097E-5 5 0.0 0.0 0.0 0.0 3.647663434472097E-5 6 0.0 0.0 0.0 0.0 7.295326868944194E-5 7 0.0 3.647663434472097E-5 0.0 0.0 7.295326868944194E-5 8 0.0 3.647663434472097E-5 0.0 0.0 7.295326868944194E-5 9 0.0 3.647663434472097E-5 0.0 0.0 7.295326868944194E-5 10-11 0.0 3.647663434472097E-5 0.0 0.0 7.295326868944194E-5 12-13 0.0 3.647663434472097E-5 0.0 1.8238317172360486E-5 9.119158586180243E-5 14-15 0.0 3.647663434472097E-5 0.0 3.647663434472097E-5 1.0942990303416292E-4 16-17 0.0 3.647663434472097E-5 0.0 5.4714951517081454E-5 1.4590653737888389E-4 18-19 0.0 3.647663434472097E-5 0.0 7.295326868944194E-5 1.4590653737888389E-4 20-21 0.0 3.647663434472097E-5 0.0 7.295326868944194E-5 1.4590653737888389E-4 22-23 0.0 3.647663434472097E-5 0.0 9.119158586180243E-5 1.6414485455124438E-4 24-25 0.0 3.647663434472097E-5 0.0 1.8238317172360486E-4 1.8238317172360486E-4 26-27 0.0 3.647663434472097E-5 0.0 2.1885980606832584E-4 1.8238317172360486E-4 28-29 0.0 3.647663434472097E-5 0.0 4.924345636537331E-4 1.8238317172360486E-4 30-31 0.0 3.647663434472097E-5 0.0 7.660093212391404E-4 1.8238317172360486E-4 32-33 0.0 3.647663434472097E-5 0.0 0.00149554200813356 1.8238317172360486E-4 34-35 0.0 3.647663434472097E-5 0.0 0.0021521214263385373 2.553364404130468E-4 36-37 0.0 3.647663434472097E-5 0.0 0.002845177478888236 2.553364404130468E-4 38-39 0.0 3.647663434472097E-5 0.0 0.0036111868001273764 2.553364404130468E-4 40-41 0.0 3.647663434472097E-5 0.0 0.004851392367847889 2.9181307475776777E-4 42-43 0.0 3.647663434472097E-5 0.0 0.007021752111358788 2.9181307475776777E-4 44-45 0.0 3.647663434472097E-5 0.0 0.01231086409134333 2.9181307475776777E-4 46-47 0.0 3.647663434472097E-5 0.0 0.023162662808897817 2.9181307475776777E-4 48-49 0.0 3.647663434472097E-5 0.0 0.031953531685975575 2.9181307475776777E-4 50-51 0.0 3.647663434472097E-5 0.0 0.042440564060082855 2.9181307475776777E-4 52-53 0.0 5.4714951517081454E-5 0.0 0.05507971786052867 2.9181307475776777E-4 54-55 0.0 7.295326868944194E-5 0.0 0.07213254441668573 2.9181307475776777E-4 56-57 0.0 7.295326868944194E-5 0.0 0.09051676812642509 2.9181307475776777E-4 58-59 0.0 1.4590653737888389E-4 0.0 0.11572212245862729 2.9181307475776777E-4 60-61 0.0 1.4590653737888389E-4 0.0 0.14800394385370536 2.9181307475776777E-4 62-63 0.0 1.4590653737888389E-4 0.0 0.18849300797634563 2.9181307475776777E-4 64-65 0.0 1.4590653737888389E-4 0.0 0.21705421266826214 2.9181307475776777E-4 66-67 0.0 1.4590653737888389E-4 0.0 0.2728634632156853 3.1005139193012826E-4 68-69 0.0 1.4590653737888389E-4 0.0 0.3439199469192017 3.2828970910248875E-4 70-71 0.0 1.6414485455124438E-4 0.0 0.3862146044419057 3.6476634344720973E-4 72-73 0.0 1.8238317172360486E-4 0.0 0.46981905036000615 4.7419624648137266E-4 74-75 0.0 1.8238317172360486E-4 0.0 0.5323217633096855 4.7419624648137266E-4 76-77 0.0 1.8238317172360486E-4 0.0 0.5879303923682127 4.7419624648137266E-4 78-79 0.0 1.8238317172360486E-4 0.0 0.6611025208637229 4.7419624648137266E-4 80-81 0.0 2.1885980606832584E-4 0.0 0.7379587894280499 4.924345636537331E-4 82-83 0.0 2.1885980606832584E-4 0.0 0.8421907720680902 5.836261495155355E-4 84-85 0.0 2.1885980606832584E-4 0.0 0.9932405148895798 5.836261495155355E-4 86-87 0.0 2.1885980606832584E-4 0.0 1.212975760182179 5.836261495155355E-4 88-89 0.0 2.1885980606832584E-4 0.0 1.479510527339055 5.836261495155355E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10335 0.0 23.269846 1 TACATGG 10110 0.0 23.081709 2 ACATGGG 10350 0.0 21.58217 3 TTATTCG 185 6.5247223E-6 20.55246 4 GAGTACT 5835 0.0 20.357563 12-13 GTACTTT 6025 0.0 19.357698 14-15 GTATAGG 765 0.0 19.259888 1 TAAGGTG 1260 0.0 19.236998 5 GTATAGC 875 0.0 19.011372 1 CATGGGG 5925 0.0 18.690165 4 AGAGTAC 11080 0.0 18.249287 10-11 AGTACTT 6245 0.0 18.069986 12-13 GTACCGT 290 5.1863026E-8 18.027695 6 GTGTAGC 1660 0.0 17.751583 1 AGGTAAG 1180 0.0 17.721819 2 GGTAAGG 1220 0.0 17.530338 3 TACCGTA 220 2.9082554E-5 17.280542 7 GTATCAA 17315 0.0 17.155802 1 GTAAGGT 1310 0.0 16.689068 4 ACTTTTT 7405 0.0 16.42427 16-17 >>END_MODULE