FastQCFastQC Report
Thu 26 May 2016
SRR936458_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936458_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2741481
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT85620.312312943259501No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47300.1725344804505302No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT40980.14948124754466655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40610.14813161207391187No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT34280.1250419025337035No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC31360.11439072530504497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG12300.022.7785425
GTACATG105700.021.9806251
TACATGG102500.021.68962
ACATGGG102050.021.270093
GAGTACT73100.020.65802412-13
GTACTTT73650.020.24584614-15
GTAAGGT11150.019.1684934
AGAGTAC128350.018.11072510-11
AGTACTT77950.017.48416112-13
GTATAGC10500.017.3725661
CATGGGG61300.017.278054
GGTAAGG12000.016.623953
ACTTTTT91650.016.50273916-17
GTATCAA194850.016.4075411
GAGTACA87100.016.2582421
GTGTAGC16100.016.1004331
GTATAGG6000.016.0010491
AGTACAT82100.015.8539312
TACTTTT88000.015.2985214-15
AGGTAAG12700.014.9618682
Produced by FastQC (version 0.11.5)