##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936458_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2741481 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.526820722084157 31.0 31.0 34.0 30.0 34.0 2 31.74439764492258 31.0 31.0 34.0 30.0 34.0 3 32.043194171325645 33.0 31.0 34.0 30.0 34.0 4 35.825342579430604 37.0 35.0 37.0 35.0 37.0 5 35.63031514717775 37.0 35.0 37.0 33.0 37.0 6 35.72091435249779 37.0 35.0 37.0 33.0 37.0 7 35.65939650867542 37.0 35.0 37.0 33.0 37.0 8 35.63470583965382 37.0 35.0 37.0 33.0 37.0 9 37.28361312735707 39.0 37.0 39.0 34.0 39.0 10-11 37.16845912847837 39.0 37.0 39.0 33.0 39.0 12-13 37.046348670663775 39.0 37.0 39.0 33.0 39.0 14-15 38.20755514993539 40.0 37.5 41.0 33.0 41.0 16-17 38.11510347874014 40.0 37.0 41.0 33.0 41.0 18-19 38.14298512373422 40.0 37.5 41.0 33.0 41.0 20-21 38.198563294803066 40.0 38.0 41.0 33.0 41.0 22-23 38.11541736017868 40.0 37.0 41.0 33.0 41.0 24-25 38.01353173704286 40.0 37.0 41.0 33.0 41.0 26-27 37.813598927003326 40.0 37.0 41.0 33.0 41.0 28-29 37.75848729208774 40.0 37.0 41.0 32.5 41.0 30-31 37.79914816115814 40.0 37.0 41.0 33.0 41.0 32-33 37.671794369539676 40.0 37.0 41.0 32.5 41.0 34-35 37.52946527807415 40.0 37.0 41.0 32.0 41.0 36-37 37.31933779588478 39.5 36.0 41.0 31.5 41.0 38-39 37.181709630670426 39.0 36.0 41.0 31.0 41.0 40-41 36.98279579541131 39.0 36.0 41.0 31.0 41.0 42-43 36.78007945340492 39.0 35.5 41.0 30.5 41.0 44-45 36.5592130676813 39.0 35.0 40.5 30.0 41.0 46-47 36.25986993891258 39.0 35.0 40.0 30.0 41.0 48-49 35.88274494698304 38.5 35.0 40.0 29.0 41.0 50-51 35.70633372983435 38.0 35.0 40.0 28.0 41.0 52-53 35.44363885797494 38.0 34.0 40.0 28.0 41.0 54-55 35.1755126152616 38.0 34.0 40.0 27.0 41.0 56-57 34.881295000767835 38.0 34.0 40.0 26.5 41.0 58-59 34.51465394069848 37.5 33.5 40.0 26.0 41.0 60-61 34.12825877691657 37.0 33.0 40.0 26.0 41.0 62-63 33.835389703594515 36.0 33.0 39.0 26.0 40.5 64-65 33.38871106529646 36.0 32.0 39.0 24.5 40.0 66-67 32.976494821594606 35.0 32.0 38.5 23.5 40.0 68-69 32.513922584179866 35.0 31.0 38.0 22.5 40.0 70-71 32.03243046368003 35.0 31.0 37.0 22.0 39.5 72-73 31.614955201221527 34.5 31.0 37.0 21.0 39.0 74-75 31.24762181463231 34.0 30.0 36.0 20.0 39.0 76-77 29.808818299306104 32.5 28.5 35.0 19.5 37.0 78-79 30.626647968743903 34.0 30.0 35.0 20.0 37.0 80-81 30.48086946435157 34.0 30.0 35.0 18.5 37.0 82-83 30.152802080335412 34.0 30.0 35.0 17.5 36.5 84-85 29.716319390869387 34.0 29.0 35.0 14.0 36.0 86-87 29.351813125825053 34.0 29.0 35.0 8.5 36.0 88-89 28.94698248866215 33.5 29.0 35.0 6.5 35.0 90-91 28.502461260902408 33.0 29.0 35.0 2.0 35.0 92-93 28.047693381788896 33.0 27.0 35.0 2.0 35.0 94-95 27.519631542221155 33.0 27.0 34.5 2.0 35.0 96-97 26.840428403479727 32.5 25.5 34.0 2.0 35.0 98-99 25.98910059927463 32.0 24.5 34.0 2.0 35.0 100-101 23.12915683165413 29.5 10.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.46851053953627897 1101 2 0.4859171186868956 1101 3 0.2755167221993702 1101 4 0.21039751164287424 1101 5 0.28556700484541864 1101 6 0.25033323566500343 1101 7 0.3133947526065626 1101 8 0.2510195732680671 1101 9 0.36328998808239277 1101 10-11 0.38456523920802255 1101 12-13 0.35400649604383005 1101 14-15 0.4824033883295513 1101 16-17 0.40690611230497353 1101 18-19 0.4051165498587963 1101 20-21 0.2876676512094747 1101 22-23 0.2211432246272409 1101 24-25 0.21685189072423583 1101 26-27 0.31016407844324334 1101 28-29 0.4282601047028294 1101 30-31 0.23480774518274217 1101 32-33 0.3292288254214881 1101 34-35 0.4280170446862286 1101 36-37 0.4210908847601118 1101 38-39 0.44334881254882674 1101 40-41 0.5293494019157734 1101 42-43 0.6385795247664205 1101 44-45 0.6305240360338686 1101 46-47 0.6812391598254166 1101 48-49 0.5342778200485157 1101 50-51 0.4878373947827015 1101 52-53 0.6038295207953936 1101 54-55 0.5730608240009261 1101 56-57 0.5682412577255391 1101 58-59 0.5716241573725327 1101 60-61 0.7106580998150562 1101 62-63 0.5204958452094033 1101 64-65 0.5822092119138276 1101 66-67 0.4527056912449652 1101 68-69 0.29549795037085147 1101 70-71 0.16529628170403754 1101 72-73 0.3453238355136641 1101 74-75 0.27514279091782967 1101 76-77 0.634599904724837 1101 78-79 0.5854187412787617 1101 80-81 0.43193066685791237 1101 82-83 0.45959726018219627 1101 84-85 0.5233659724851272 1101 86-87 0.46683708171683236 1101 88-89 0.5304251912662821 1101 90-91 0.4586265256195148 1101 92-93 0.49401761465048466 1101 94-95 0.07646299430116343 1101 96-97 -0.0207365992301618 1101 98-99 0.03423579405519561 1101 100-101 -0.027780510982093887 1102 1 0.42567539544381106 1102 2 0.4836847258649293 1102 3 0.2423764184460211 1102 4 0.13812717960178844 1102 5 0.13505510665003584 1102 6 0.1529807775067411 1102 7 0.14579588224108164 1102 8 0.048871481140977835 1102 9 0.13118064250267025 1102 10-11 0.24752953759028884 1102 12-13 0.2698505342893114 1102 14-15 0.3460524833666767 1102 16-17 0.4325493315550162 1102 18-19 0.32239886942223706 1102 20-21 0.3049594503691253 1102 22-23 0.14276024581518243 1102 24-25 0.1135496838013168 1102 26-27 -0.12463049297743822 1102 28-29 0.06110251735382377 1102 30-31 0.19438690319667984 1102 32-33 0.28631093018353226 1102 34-35 0.38547507794086044 1102 36-37 0.41798840042878993 1102 38-39 0.3220305548986033 1102 40-41 0.3721241135823945 1102 42-43 0.20543912986311597 1102 44-45 0.14141268046723354 1102 46-47 0.24580784080210805 1102 48-49 0.09904240833975564 1102 50-51 0.09521446082683838 1102 52-53 -0.0010334990393587873 1102 54-55 0.0817485223497556 1102 56-57 0.023175674024606963 1102 58-59 0.03332190096821108 1102 60-61 0.2613266717763949 1102 62-63 0.03995226068239077 1102 64-65 0.004222605229038834 1102 66-67 0.021637200120622424 1102 68-69 0.1116030941976831 1102 70-71 0.03734054881406479 1102 72-73 0.13518514693767258 1102 74-75 -0.3415335246235536 1102 76-77 -0.09288071549076804 1102 78-79 0.02611025959235036 1102 80-81 0.11938837978552996 1102 82-83 0.10886190134041485 1102 84-85 0.09883605889008251 1102 86-87 0.035461805857977424 1102 88-89 0.16598862565107098 1102 90-91 0.14663367750608458 1102 92-93 0.2936464259559983 1102 94-95 0.1386720631495919 1102 96-97 0.06844419958728665 1102 98-99 -0.3790768143807064 1102 100-101 -0.4505658793816032 1103 1 0.5187773994591645 1103 2 0.5469297562437525 1103 3 0.2954822768820691 1103 4 0.15768392861480152 1103 5 0.13439265889820007 1103 6 0.1390050522244124 1103 7 0.1889360669550726 1103 8 0.18873717035218363 1103 9 0.10835318093813129 1103 10-11 0.20470554352569792 1103 12-13 0.23851420490787234 1103 14-15 0.1976841491085466 1103 16-17 0.09524001786739689 1103 18-19 0.16667312624174713 1103 20-21 0.19698231516730402 1103 22-23 0.15631812133440093 1103 24-25 0.08780861042648525 1103 26-27 0.08327545620015542 1103 28-29 0.07492193404738146 1103 30-31 0.0936136408497461 1103 32-33 -0.00857548085174642 1103 34-35 0.03720906820154113 1103 36-37 0.08035889133880403 1103 38-39 0.20741132126537565 1103 40-41 0.36001614347091504 1103 42-43 0.30385802874827306 1103 44-45 0.259475015864183 1103 46-47 0.20091881127450506 1103 48-49 0.38820947763812796 1103 50-51 0.19452001201824487 1103 52-53 0.18178113252891137 1103 54-55 0.18132122136965023 1103 56-57 0.4313999825793644 1103 58-59 0.5092341128400975 1103 60-61 0.5753310341229891 1103 62-63 0.22031019194776746 1103 64-65 0.2505142964017111 1103 66-67 0.2030610734018623 1103 68-69 0.24754221554501754 1103 70-71 0.20259156611373186 1103 72-73 0.1677755185011165 1103 74-75 0.3240045410462642 1103 76-77 0.2348399873692557 1103 78-79 0.4048383919468499 1103 80-81 0.2911501396193863 1103 82-83 0.27513154295938236 1103 84-85 0.36455363607122493 1103 86-87 0.25613540037760174 1103 88-89 0.25919697126051133 1103 90-91 0.2387743559753126 1103 92-93 0.4808760820265796 1103 94-95 0.3739940582263337 1103 96-97 0.14891311796019124 1103 98-99 -0.05827349050968067 1103 100-101 0.1659986784067975 1104 1 0.26821036049796376 1104 2 0.3690859613023285 1104 3 0.16995865420697243 1104 4 0.07142560916379637 1104 5 0.05971308444845391 1104 6 0.15108229040595234 1104 7 0.10852726454871942 1104 8 0.05921907123801162 1104 9 0.10577735576745795 1104 10-11 0.0621954510574696 1104 12-13 0.06041308868925199 1104 14-15 0.27061460384761205 1104 16-17 0.04426707151264253 1104 18-19 0.09318219322471322 1104 20-21 0.09549145482246502 1104 22-23 0.12280917939033031 1104 24-25 0.13107248680586991 1104 26-27 0.09936403266725335 1104 28-29 0.02122758066416708 1104 30-31 0.0065549774170676756 1104 32-33 0.10205200664168501 1104 34-35 0.1898114507804607 1104 36-37 0.14625979664183575 1104 38-39 0.11402121259077802 1104 40-41 0.10891071287576182 1104 42-43 0.04016184549848134 1104 44-45 0.16622039568856195 1104 46-47 0.1367464827283129 1104 48-49 0.1470165566212671 1104 50-51 0.1769029447897026 1104 52-53 0.14485944784995297 1104 54-55 0.20658584845745054 1104 56-57 0.3080286202407265 1104 58-59 0.24893909464889674 1104 60-61 0.09179977398139272 1104 62-63 -0.19648615458881835 1104 64-65 -0.008287201547723555 1104 66-67 0.09046555905676001 1104 68-69 -0.03276223340568407 1104 70-71 -0.03532582247547822 1104 72-73 -0.08363027857796368 1104 74-75 0.02295996153025115 1104 76-77 0.21533403709674204 1104 78-79 0.30420392341147107 1104 80-81 0.2780990110571864 1104 82-83 0.30348853769860895 1104 84-85 0.32585112666035343 1104 86-87 0.37470262078176475 1104 88-89 0.2500683594262725 1104 90-91 0.088102357956938 1104 92-93 0.1692278353439427 1104 94-95 0.31014287660869044 1104 96-97 0.03745191902828182 1104 98-99 0.21943767769685607 1104 100-101 0.2523161330659498 1105 1 0.32394436985644504 1105 2 0.3264713628569389 1105 3 0.11805418645470667 1105 4 0.07127624095890894 1105 5 -0.09067050027507406 1105 6 -0.024439349992462667 1105 7 0.02567824016838216 1105 8 -0.009318026282066683 1105 9 -0.03243925728593666 1105 10-11 -0.025762984086703966 1105 12-13 -0.028305377235646745 1105 14-15 0.017102513064138236 1105 16-17 0.0472560554050645 1105 18-19 0.026192173126538876 1105 20-21 0.10095675760662459 1105 22-23 0.08885649058331069 1105 24-25 -0.04629809131387219 1105 26-27 -0.13392885593680148 1105 28-29 0.02053326726560556 1105 30-31 -0.07337037550436065 1105 32-33 -0.06693717555987178 1105 34-35 -0.1313618914262662 1105 36-37 0.005915236241953892 1105 38-39 -0.005614215603664263 1105 40-41 -0.055115649976059444 1105 42-43 -0.05113504422971715 1105 44-45 -0.22318673749362006 1105 46-47 -0.12246392986693166 1105 48-49 -0.10998538169440053 1105 50-51 -0.30121137360475814 1105 52-53 -0.23151892506864868 1105 54-55 -0.1467588134109974 1105 56-57 -0.23447293967935678 1105 58-59 -0.12212288089197187 1105 60-61 -0.2513087692667213 1105 62-63 -0.2910005928018151 1105 64-65 -0.11370239482798894 1105 66-67 -0.13224688886828773 1105 68-69 -0.27165290085478233 1105 70-71 -0.2192712048517187 1105 72-73 -0.3625614594047768 1105 74-75 -0.31898313948095236 1105 76-77 -0.37139595736934794 1105 78-79 -0.42013468262334897 1105 80-81 -0.48295640547242513 1105 82-83 -0.16635210112062637 1105 84-85 -0.10846961419668943 1105 86-87 -0.23414017363701944 1105 88-89 -0.1353326745349186 1105 90-91 -0.3644968397125048 1105 92-93 -0.33238663710456606 1105 94-95 -0.11841561839846548 1105 96-97 -0.21095926491316774 1105 98-99 -0.17934060377277206 1105 100-101 0.08977963417568091 1106 1 0.33859127336423 1106 2 0.37605464915636944 1106 3 0.19422419383965206 1106 4 0.0776112629960366 1106 5 -0.076083654709997 1106 6 -0.02454350401548311 1106 7 -0.0027246882247524695 1106 8 0.04038939507676531 1106 9 0.05256650219038761 1106 10-11 4.1269033356883256E-4 1106 12-13 0.07101857473315931 1106 14-15 0.0958624761658271 1106 16-17 0.08427505932284163 1106 18-19 -0.03126720134989114 1106 20-21 -0.01801756756545103 1106 22-23 -0.03670411241680682 1106 24-25 -0.06384798677515136 1106 26-27 0.004023779131273386 1106 28-29 -0.08654539382581561 1106 30-31 -0.04494149473062947 1106 32-33 -0.049298858270887536 1106 34-35 -0.1627190275328374 1106 36-37 -0.024017734270977087 1106 38-39 -7.069189999953096E-4 1106 40-41 0.05101448605461201 1106 42-43 -0.08851456805405888 1106 44-45 -0.03348442393075146 1106 46-47 0.07205507663460509 1106 48-49 -0.17290095710748687 1106 50-51 -0.24380625797714828 1106 52-53 -0.041950622249842695 1106 54-55 -0.22523157782298142 1106 56-57 -0.17624957764491 1106 58-59 -0.28235701270502744 1106 60-61 -0.02452102058176564 1106 62-63 -0.47733455148034665 1106 64-65 -0.16406034820487747 1106 66-67 -0.209346071862079 1106 68-69 -0.16271864455068652 1106 70-71 -0.12074132682905514 1106 72-73 -0.08047487192808589 1106 74-75 -0.0767626813571276 1106 76-77 -0.11853123369387575 1106 78-79 -0.05040305894711139 1106 80-81 -0.02464488572742951 1106 82-83 0.12787399946413913 1106 84-85 0.10676128677465613 1106 86-87 -0.023406958023382884 1106 88-89 -0.08271824204860323 1106 90-91 -0.1881682701721239 1106 92-93 0.2460563854199158 1106 94-95 0.17069131769678236 1106 96-97 0.21713495646775982 1106 98-99 0.11937583282297837 1106 100-101 0.32405671166661776 1107 1 0.5853413402583918 1107 2 0.6068177711481972 1107 3 0.4436706405208781 1107 4 0.21205339872016538 1107 5 0.0930487032348779 1107 6 0.21357407775231252 1107 7 0.17874024436905955 1107 8 0.2205681098499639 1107 9 0.32883562483629447 1107 10-11 0.3656764845704217 1107 12-13 0.37487068743248386 1107 14-15 0.5264310930588323 1107 16-17 0.43557247572164925 1107 18-19 0.41404389779551565 1107 20-21 0.3831213179282571 1107 22-23 0.41311581840967904 1107 24-25 0.3634639250104357 1107 26-27 0.4240615041351532 1107 28-29 0.5321315883060151 1107 30-31 0.49333451883979507 1107 32-33 0.5640909414430411 1107 34-35 0.4906195692423623 1107 36-37 0.6139811888156075 1107 38-39 0.694967456737082 1107 40-41 0.6924181733268853 1107 42-43 0.5104981732191405 1107 44-45 0.4130854086191178 1107 46-47 0.5331064216201824 1107 48-49 0.5212663344552624 1107 50-51 0.45372212661969513 1107 52-53 0.2574260893322631 1107 54-55 0.3924482353358414 1107 56-57 0.4771445825773455 1107 58-59 0.28007601880624833 1107 60-61 0.4684538040894637 1107 62-63 0.33672471839878426 1107 64-65 0.41560528008820796 1107 66-67 0.3913669373181108 1107 68-69 0.4061985289149632 1107 70-71 0.3511943754935771 1107 72-73 0.3858910077549176 1107 74-75 0.6702683079827487 1107 76-77 0.4734217785254593 1107 78-79 0.5998782929426092 1107 80-81 0.5324043054313101 1107 82-83 0.4674575709186648 1107 84-85 0.6524799613122596 1107 86-87 0.7833004287437966 1107 88-89 0.8686466300927052 1107 90-91 0.8648738781049801 1107 92-93 1.0002199771574176 1107 94-95 1.05051465104113 1107 96-97 1.1236611178660318 1107 98-99 1.0954476094328136 1107 100-101 1.2420393158811152 1108 1 0.37793849881546393 1108 2 0.3718868142253804 1108 3 0.14548512106069467 1108 4 0.1976261003686517 1108 5 0.0244416651117092 1108 6 0.05234254855491827 1108 7 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2316 72-73 -1.5148837000903441 2316 74-75 -1.4442984378735275 2316 76-77 -1.3837004115021436 2316 78-79 -1.2187177851117994 2316 80-81 -1.3064849572813664 2316 82-83 -1.123328191430108 2316 84-85 -1.3477127715532582 2316 86-87 -1.3429838161855479 2316 88-89 -1.3602919315859303 2316 90-91 -1.5600928897229842 2316 92-93 -1.7497981494818795 2316 94-95 -1.886744038073278 2316 96-97 -1.9004880286713721 2316 98-99 -2.3104296100505763 2316 100-101 -3.0749430106455726 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 12.0 4 41.0 5 96.0 6 161.0 7 398.0 8 1039.0 9 1850.0 10 2927.0 11 4515.0 12 6270.0 13 8593.0 14 10683.0 15 13651.0 16 16500.0 17 19764.0 18 18204.0 19 14634.0 20 14835.0 21 15662.0 22 17136.0 23 19383.0 24 23379.0 25 28371.0 26 35495.0 27 43704.0 28 54833.0 29 68077.0 30 84201.0 31 104555.0 32 131002.0 33 165951.0 34 217508.0 35 293741.0 36 417357.0 37 537926.0 38 329229.0 39 19796.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.990646282949754 18.634013798004236 14.746128663920038 23.629211255125966 2 15.92660405875265 19.982766589802157 38.87184639355141 25.218782957893787 3 20.34708307296996 22.919746797302505 28.989323186548578 27.74384694317895 4 13.652874833781144 16.223215735202643 35.9011205294196 34.222788901596616 5 14.495340292345634 36.797446343782795 33.827810588510374 14.8794027753612 6 33.83700270036524 35.00352546670942 16.568270945521782 14.591200887403561 7 29.98835301065373 31.55907336217176 19.740352021407407 18.712221605767102 8 27.06941977712047 33.99312269536065 19.127143321438304 19.81031420608058 9 26.204847734502874 16.67415077828073 19.73970612428927 37.38129536292713 10-11 25.801695513102274 25.736055570166528 26.645585526894138 21.816663389837064 12-13 26.672713292192444 22.799213124034733 27.05163353728687 23.47644004648594 14-15 23.247866708468912 24.685685229453657 25.282406817194875 26.784041244882555 16-17 23.145682935610353 27.597273152722927 26.066111711151745 23.190932200514975 18-19 23.049895293821113 26.62615571656342 27.762457591352995 22.561491398262472 20-21 23.864436777055907 25.584528946215567 27.63962252519715 22.91141175153138 22-23 23.404097768948688 25.506986649002005 27.721422623049246 23.36749295900006 24-25 23.576326080684126 25.600870478402005 27.847904107305503 22.974899333608366 26-27 23.239600507754936 25.740822475451214 27.892397537096752 23.127179479697098 28-29 22.95746350239159 26.361025299828817 27.730759396107434 22.950751801672162 30-31 23.251555091248193 25.700506916157302 27.5377998518318 23.510138140762706 32-33 23.18002453159697 25.692205974219025 27.711968045087072 23.415801449096936 34-35 23.81655776797968 25.733727891590714 27.421659059717214 23.028055280712394 36-37 23.741203099536637 25.543526931259926 27.57717607297791 23.138093896225527 38-39 23.294261279563706 25.825837820427587 27.721497216733994 23.158403683274706 40-41 23.574919264849594 25.792149256704032 27.45431027029906 23.17862120814731 42-43 23.358603937588544 25.797138511280938 27.15561513215675 23.688642418973764 44-45 23.53019818169449 25.660625407068693 27.277847419847934 23.531328991388882 46-47 24.018199264288942 26.080280379269283 26.8695948591784 23.03192549726338 48-49 23.941295385204118 25.98834349214792 26.777354790238128 23.29300633240984 50-51 23.511696920210127 26.210833277554247 27.12050963908225 23.156960163153375 52-53 23.938013876020456 26.282619956081156 26.5367583223293 23.242607845569086 54-55 23.52171869348389 26.06888921032169 26.72446012209623 23.68493197409819 56-57 23.731761505228352 26.177215873265432 26.56769845092831 23.523324170577904 58-59 24.153991726224934 25.884309410443368 26.47093691510973 23.49076194822197 60-61 23.68457291607958 26.08063931582963 27.118192408689946 23.11659535940084 62-63 23.372997127353973 26.122730381651543 27.10276777164744 23.401504719347045 64-65 23.358013881668356 26.378197936903163 26.780018423764833 23.483769757663648 66-67 23.474886813331324 26.227115299839117 26.457400902502155 23.840596984327412 68-69 23.56700862457632 25.997312388836928 26.787564180942475 23.648114805644273 70-71 23.707934659755686 26.006785863386483 26.584184302050623 23.701095174807204 72-73 23.633671660258024 25.899782982224384 26.864808996452826 23.60173636106476 74-75 23.432079230167925 26.57904614330721 26.4169257419621 23.57194888456276 76-77 23.688703431916576 26.120239369109083 26.42361824818304 23.767438950791302 78-79 23.63677321445509 26.179200198454676 26.578827366316695 23.605199220773535 80-81 23.83004672261317 26.102060107162494 26.463761792379692 23.604131377844645 82-83 23.746839829998358 26.15446071904128 26.5843168013425 23.51438264961786 84-85 23.621274275010077 26.342410109275168 26.440796061643525 23.595519554071235 86-87 23.550810683622714 26.370537774445914 26.35709478130968 23.7215567606217 88-89 23.526024963664057 26.426205151996164 26.06312849049902 23.984641393840757 90-91 23.57442686470778 26.09304794689229 26.367707768954464 23.964817419445463 92-93 23.686156808150013 26.082108941270583 26.576437648795164 23.655296601784237 94-95 23.43344761815821 26.040524206014737 26.676420122859984 23.849608052967067 96-97 23.22264464676154 25.94372911146323 26.94940676236367 23.884219479411563 98-99 23.13613467327591 26.23945174616192 26.3719661230142 24.252447457547976 100-101 23.05975739819496 25.909173074085285 26.581571259013298 24.44949826870646 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 90.0 1 68.0 2 72.0 3 103.0 4 93.0 5 92.0 6 158.5 7 331.5 8 521.5 9 648.0 10 701.0 11 709.5 12 794.5 13 894.5 14 990.5 15 1177.5 16 1400.0 17 1604.0 18 1794.5 19 2054.5 20 2448.0 21 2854.0 22 3373.0 23 4149.5 24 5127.0 25 6466.5 26 8524.0 27 11265.0 28 14308.0 29 18047.5 30 22632.5 31 27611.5 32 33782.0 33 38664.5 34 42598.0 35 47467.5 36 54107.0 37 60388.0 38 64335.5 39 67877.0 40 71844.0 41 76464.0 42 80028.5 43 84575.0 44 90125.5 45 95570.5 46 100839.5 47 109638.5 48 124049.0 49 123790.5 50 110139.5 51 103734.5 52 103811.0 53 104935.0 54 105038.5 55 99378.5 56 89961.0 57 82594.0 58 77770.0 59 70504.0 60 59558.0 61 49777.0 62 41222.0 63 32536.0 64 23564.5 65 17498.0 66 13911.5 67 11214.5 68 8660.5 69 6740.5 70 5326.0 71 3923.5 72 3046.5 73 2544.5 74 1999.5 75 1491.0 76 1112.0 77 716.5 78 499.0 79 369.0 80 245.0 81 158.0 82 117.0 83 73.0 84 45.5 85 28.5 86 13.0 87 7.0 88 5.0 89 3.5 90 3.0 91 2.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0335654341576688 2 0.010724130497347967 3 0.0043771961213665165 4 0.013350448170167877 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004304242852677075 10-11 3.647663434472098E-4 12-13 2.553364404130468E-4 14-15 5.471495151708146E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001386112105099397 24-25 0.0 26-27 3.647663434472098E-5 28-29 0.0 30-31 7.295326868944196E-5 32-33 0.004322481169849435 34-35 6.74817735377338E-4 36-37 0.003775331654678621 38-39 2.735747575854073E-4 40-41 0.004012429777919308 42-43 0.010414079105417837 44-45 0.00300932233343948 46-47 0.0018785466687531302 48-49 0.009684546418523418 50-51 4.194812949642912E-4 52-53 0.0022250746950279797 54-55 0.010304649202383675 56-57 0.0047237241476413665 58-59 0.0017508784485466067 60-61 0.006401649327498531 62-63 0.003866523240540423 64-65 0.016633345261192763 66-67 0.0037388550203339 68-69 4.7419624648137266E-4 70-71 0.0016961634970295253 72-73 1.823831717236049E-5 74-75 0.0 76-77 8.936775414456639E-4 78-79 0.010979466937761013 80-81 0.0149554200813356 82-83 0.012073765968102642 84-85 0.00860848570535415 86-87 0.010067551079142988 88-89 0.026901517829231718 90-91 0.023199139443242537 92-93 0.003155228870818364 94-95 0.008407864216458186 96-97 0.010742368814520327 98-99 0.009812214638729942 100-101 0.011763714576172514 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2741481.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.88728857469343 #Duplication Level Percentage of deduplicated Percentage of total 1 75.93104254549361 41.676490439718314 2 13.71046679684103 15.050606951439324 3 4.4196325417217786 7.277449401347671 4 2.004939296606014 4.401827269902285 5 1.0664959457744407 2.9268535369731157 6 0.6272504848338627 2.0656847021815548 7 0.4129551759398316 1.5866192937155983 8 0.29387678069581236 1.290407973396236 9 0.2044825210526607 1.010114202734839 >10 1.1916538781041284 11.971043052731886 >50 0.08924384013739478 3.3578361428656014 >100 0.04343142159142923 4.196985270908324 >500 0.0030635805231437846 1.1718706300585617 >1k 0.0013985911083627394 1.703229775285487 >5k 6.659957658870186E-5 0.31298135674108984 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8562 0.312312943259501 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4730 0.1725344804505302 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4098 0.14948124754466655 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4061 0.14813161207391187 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3428 0.1250419025337035 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3136 0.11439072530504497 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.647663434472097E-5 2 0.0 0.0 0.0 0.0 3.647663434472097E-5 3 0.0 0.0 0.0 0.0 3.647663434472097E-5 4 0.0 0.0 0.0 0.0 3.647663434472097E-5 5 0.0 3.647663434472097E-5 0.0 0.0 3.647663434472097E-5 6 0.0 3.647663434472097E-5 0.0 0.0 3.647663434472097E-5 7 0.0 3.647663434472097E-5 0.0 0.0 3.647663434472097E-5 8 0.0 3.647663434472097E-5 0.0 0.0 3.647663434472097E-5 9 0.0 3.647663434472097E-5 0.0 3.647663434472097E-5 3.647663434472097E-5 10-11 0.0 3.647663434472097E-5 0.0 3.647663434472097E-5 3.647663434472097E-5 12-13 0.0 7.295326868944194E-5 0.0 3.647663434472097E-5 3.647663434472097E-5 14-15 0.0 7.295326868944194E-5 0.0 3.647663434472097E-5 3.647663434472097E-5 16-17 0.0 7.295326868944194E-5 0.0 7.295326868944194E-5 7.295326868944194E-5 18-19 0.0 7.295326868944194E-5 0.0 1.8238317172360486E-4 7.295326868944194E-5 20-21 0.0 7.295326868944194E-5 0.0 1.8238317172360486E-4 7.295326868944194E-5 22-23 0.0 7.295326868944194E-5 0.0 2.0062148889596535E-4 7.295326868944194E-5 24-25 0.0 7.295326868944194E-5 0.0 2.9181307475776777E-4 7.295326868944194E-5 26-27 0.0 7.295326868944194E-5 0.0 3.2828970910248875E-4 7.295326868944194E-5 28-29 0.0 7.295326868944194E-5 0.0 6.01864466687896E-4 7.295326868944194E-5 30-31 0.0 1.0942990303416292E-4 0.0 8.936775414456638E-4 7.295326868944194E-5 32-33 0.0 1.0942990303416292E-4 0.0 0.0016414485455124438 7.295326868944194E-5 34-35 0.0 1.0942990303416292E-4 0.0 0.002298027963717421 1.0942990303416292E-4 36-37 0.0 1.0942990303416292E-4 0.0 0.002954607381922399 1.0942990303416292E-4 38-39 0.0 1.4590653737888389E-4 0.0 0.0037206167031615394 1.0942990303416292E-4 40-41 0.0 1.8238317172360486E-4 0.0 0.004924345636537332 1.0942990303416292E-4 42-43 0.0 1.8238317172360486E-4 0.0 0.007003513794186428 1.0942990303416292E-4 44-45 0.0 2.0062148889596535E-4 0.0 0.012219672505481526 1.276682202065234E-4 46-47 0.0 2.370981232406863E-4 0.0 0.022998517954346574 1.4590653737888389E-4 48-49 0.0 2.553364404130468E-4 0.0 0.031570527025356 1.4590653737888389E-4 50-51 0.0 2.9181307475776777E-4 0.0 0.0419116528620844 1.4590653737888389E-4 52-53 0.0 2.9181307475776777E-4 0.0 0.05447785339384077 1.6414485455124438E-4 54-55 0.0 3.1005139193012826E-4 0.0 0.07142125004696367 1.8238317172360486E-4 56-57 0.0 3.6476634344720973E-4 0.0 0.08958661395063472 1.8238317172360486E-4 58-59 0.0 3.6476634344720973E-4 0.0 0.11468253837980275 1.8238317172360486E-4 60-61 0.0 3.6476634344720973E-4 0.0 0.1470555513607426 1.8238317172360486E-4 62-63 0.0 3.6476634344720973E-4 0.0 0.1878181902409683 1.8238317172360486E-4 64-65 0.0 4.377196121366517E-4 0.0 0.21641587156722952 1.8238317172360486E-4 66-67 0.0 4.377196121366517E-4 0.0 0.2718968324055501 1.8238317172360486E-4 68-69 0.0 4.377196121366517E-4 0.0 0.3428074095716877 1.8238317172360486E-4 70-71 0.0 4.377196121366517E-4 0.0 0.38532092690046 1.8238317172360486E-4 72-73 0.0 4.377196121366517E-4 0.0 0.46894361113573285 2.370981232406863E-4 74-75 0.0 4.377196121366517E-4 0.0 0.5309903661561033 2.553364404130468E-4 76-77 0.0 4.377196121366517E-4 0.0 0.5865442802631133 2.735747575854073E-4 78-79 0.0 4.377196121366517E-4 0.0 0.6594428340010381 2.9181307475776777E-4 80-81 0.0 4.377196121366517E-4 0.0 0.736080242759297 2.9181307475776777E-4 82-83 0.0 4.377196121366517E-4 0.0 0.8405493235225778 2.9181307475776777E-4 84-85 0.0 4.559579293090122E-4 0.0 0.9909607252430346 2.9181307475776777E-4 86-87 0.0 4.7419624648137266E-4 0.0 1.20989348458005 2.9181307475776777E-4 88-89 1.8238317172360486E-5 4.7419624648137266E-4 0.0 1.476027008759134 2.9181307475776777E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 1230 0.0 22.778542 5 GTACATG 10570 0.0 21.980625 1 TACATGG 10250 0.0 21.6896 2 ACATGGG 10205 0.0 21.27009 3 GAGTACT 7310 0.0 20.658024 12-13 GTACTTT 7365 0.0 20.245846 14-15 GTAAGGT 1115 0.0 19.168493 4 AGAGTAC 12835 0.0 18.110725 10-11 AGTACTT 7795 0.0 17.484161 12-13 GTATAGC 1050 0.0 17.372566 1 CATGGGG 6130 0.0 17.27805 4 GGTAAGG 1200 0.0 16.62395 3 ACTTTTT 9165 0.0 16.502739 16-17 GTATCAA 19485 0.0 16.407541 1 GAGTACA 8710 0.0 16.258242 1 GTGTAGC 1610 0.0 16.100433 1 GTATAGG 600 0.0 16.001049 1 AGTACAT 8210 0.0 15.853931 2 TACTTTT 8800 0.0 15.29852 14-15 AGGTAAG 1270 0.0 14.961868 2 >>END_MODULE