FastQCFastQC Report
Thu 26 May 2016
SRR936457_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936457_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2718469
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT77550.285270863857561No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT45580.16766790425051747No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38730.14246989757837958No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT33750.12415076280067935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31780.11690403679423969No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30680.11285764156221756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG3150.024.1378145
GTACATG103150.023.9108241
TACATGG101350.023.6325862
ACATGGG101350.022.2727743
GTGTAGC16650.020.2647171
GTGTACG3404.1836756E-1119.567961
GAGTACT55900.018.40664112-13
ACCGGTC1600.001188148917.8201668
GTATCAA159800.017.8133871
CATGGGG62200.017.6502154
AGAGTAC111500.017.6450110-11
GTACTTT57950.017.59018114-15
GTATAGT7200.017.1607911
AGTACTT57700.017.09112712-13
GTATAGG6250.016.7278121
TAGCCCT19250.016.541414
GTATAGC8750.016.2933221
GTGTAAG14150.015.7847551
TCAACGC177400.015.7525974
CATGGGA41750.015.7090914
Produced by FastQC (version 0.11.5)