##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936457_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2718469 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.268008941797756 31.0 31.0 34.0 28.0 34.0 2 31.30881352702569 31.0 31.0 34.0 28.0 34.0 3 31.376840787958223 31.0 31.0 34.0 28.0 34.0 4 34.98985127290398 37.0 35.0 37.0 32.0 37.0 5 34.94883296443697 37.0 35.0 37.0 32.0 37.0 6 35.07701614401341 37.0 35.0 37.0 32.0 37.0 7 34.927036136884404 37.0 35.0 37.0 32.0 37.0 8 34.86165595414184 37.0 35.0 37.0 32.0 37.0 9 36.324529358252754 39.0 37.0 39.0 32.0 39.0 10-11 36.233709672613514 39.0 35.0 39.0 32.0 39.0 12-13 36.12572058022365 39.0 35.0 39.0 31.0 39.0 14-15 37.19123723684177 39.5 36.5 41.0 31.0 41.0 16-17 37.056343110773014 39.0 36.0 41.0 31.0 41.0 18-19 36.96801931528371 39.0 36.0 41.0 31.0 41.0 20-21 36.85394039806965 39.0 36.0 41.0 30.5 41.0 22-23 36.720658760500854 39.0 36.0 41.0 30.0 41.0 24-25 36.86739043189384 39.0 36.0 41.0 30.0 41.0 26-27 36.859544655465996 39.0 36.0 41.0 30.0 41.0 28-29 36.77586685741129 39.0 36.0 41.0 30.0 41.0 30-31 36.6110781472954 39.0 36.0 41.0 30.0 41.0 32-33 36.45708301253389 39.0 35.5 41.0 30.0 41.0 34-35 36.251311307945755 39.0 35.0 40.0 30.0 41.0 36-37 36.077986543160875 39.0 35.0 40.0 29.0 41.0 38-39 35.88211563199728 38.0 35.0 40.0 28.5 41.0 40-41 35.71688623265522 38.0 35.0 40.0 27.5 41.0 42-43 35.501632720476124 38.0 34.5 40.0 27.0 41.0 44-45 35.27277228469407 38.0 34.0 40.0 26.5 41.0 46-47 35.07089321231914 38.0 34.0 40.0 26.0 41.0 48-49 35.027015389912485 38.0 34.0 40.0 26.0 41.0 50-51 34.11814002661057 37.5 33.0 39.5 24.0 40.5 52-53 34.26648032403533 37.5 33.0 39.5 24.5 40.5 54-55 34.69244508581853 38.0 34.0 40.0 25.5 41.0 56-57 34.58558769660423 38.0 34.0 40.0 25.0 41.0 58-59 34.31171607989644 38.0 33.5 40.0 24.5 41.0 60-61 33.980230968239844 37.0 33.0 40.0 23.0 41.0 62-63 33.641532605300995 37.0 33.0 40.0 23.0 41.0 64-65 33.28583809489827 36.0 32.0 39.0 22.0 41.0 66-67 32.8821134248726 36.0 32.0 39.0 21.5 41.0 68-69 32.463017051141655 35.0 31.5 39.0 20.5 40.0 70-71 32.02287096155961 35.0 31.0 38.0 19.5 40.0 72-73 31.623495614627203 35.0 31.0 37.0 18.5 39.5 74-75 31.194155423512278 35.0 30.5 37.0 17.5 39.0 76-77 30.766836590742805 34.5 30.0 36.0 14.5 39.0 78-79 30.32442084128971 34.0 30.0 36.0 11.0 37.5 80-81 29.861910325260283 34.0 29.0 35.0 7.0 37.0 82-83 29.381495613891495 34.0 29.0 35.0 7.0 37.0 84-85 29.072823342844814 34.0 29.0 35.0 2.0 36.0 86-87 28.759309192048907 34.0 29.0 35.0 2.0 36.0 88-89 28.36317942194669 34.0 28.0 35.0 2.0 35.5 90-91 27.960320312646566 33.5 27.0 35.0 2.0 35.0 92-93 27.58641536835623 33.0 26.5 35.0 2.0 35.0 94-95 27.11225086620447 33.0 25.5 35.0 2.0 35.0 96-97 26.516378520409834 33.0 25.0 35.0 2.0 35.0 98-99 25.796252412663158 33.0 23.5 35.0 2.0 35.0 100-101 23.286358608466752 29.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.450580724517021 1101 2 0.4463649042582958 1101 3 0.43129949066078055 1101 4 0.4903749926920966 1101 5 0.5014893613237348 1101 6 0.3767630285603474 1101 7 0.4215990006633561 1101 8 0.5359716385072559 1101 9 0.6125788460520738 1101 10-11 0.6692411177915005 1101 12-13 0.7210817429335776 1101 14-15 0.9012948239157197 1101 16-17 0.9287144764814954 1101 18-19 0.9804662556692563 1101 20-21 0.849420695095084 1101 22-23 0.851304687989348 1101 24-25 0.7987420228745208 1101 26-27 0.6573096724893617 1101 28-29 0.703009437180917 1101 30-31 0.6696217996149514 1101 32-33 0.6013967686962118 1101 34-35 0.5959954112751902 1101 36-37 0.6230905177281514 1101 38-39 0.6549034368632789 1101 40-41 0.5398467399026856 1101 42-43 0.5909640967589169 1101 44-45 0.5225318973765383 1101 46-47 0.5244593416954402 1101 48-49 0.6807867272691155 1101 50-51 0.6855653378326778 1101 52-53 0.7678025860801796 1101 54-55 0.7892706866650698 1101 56-57 0.6829267379835002 1101 58-59 0.6245642339265132 1101 60-61 0.5422391040911947 1101 62-63 0.6294350776415243 1101 64-65 0.6829547469100561 1101 66-67 0.6898981606520707 1101 68-69 0.7028074909296791 1101 70-71 0.6726007666619864 1101 72-73 0.5835794264313989 1101 74-75 0.579000226811818 1101 76-77 0.6930593263451037 1101 78-79 0.5544394470021636 1101 80-81 0.6793643288681857 1101 82-83 0.7776423445698164 1101 84-85 0.6781003986466203 1101 86-87 0.4813111560634944 1101 88-89 0.4954731612606018 1101 90-91 0.5080728721384453 1101 92-93 0.502072023161606 1101 94-95 0.6200365896801188 1101 96-97 0.4339585447337946 1101 98-99 0.5364905011070462 1101 100-101 0.8148526616976675 1102 1 0.4221843426989693 1102 2 0.3990885667150472 1102 3 0.3353231239418797 1102 4 0.49114799709250434 1102 5 0.5066838305825243 1102 6 0.33830029469142175 1102 7 0.3847497004278466 1102 8 0.357004075616679 1102 9 0.6041743030721278 1102 10-11 0.5974484594283638 1102 12-13 0.47583343908169695 1102 14-15 0.6044132600205359 1102 16-17 0.5292120667330167 1102 18-19 0.666515556506333 1102 20-21 0.7138727191935743 1102 22-23 0.4829896470882815 1102 24-25 0.37280494302647327 1102 26-27 0.2889942556134031 1102 28-29 0.394409612836391 1102 30-31 0.20730225503585586 1102 32-33 0.23241362039266988 1102 34-35 0.1916298914823713 1102 36-37 0.16290262552366386 1102 38-39 0.09873591949072846 1102 40-41 0.15438711810337225 1102 42-43 0.38401598029292217 1102 44-45 0.06438066122118613 1102 46-47 0.32528939402111945 1102 48-49 0.33266379450445527 1102 50-51 0.49819147741260394 1102 52-53 0.4174136525755969 1102 54-55 0.6126939481821765 1102 56-57 0.5210824507466896 1102 58-59 0.6421162462945844 1102 60-61 0.532466889739382 1102 62-63 0.6169139854494787 1102 64-65 0.5440349027541487 1102 66-67 0.5250301579209875 1102 68-69 0.29676950944368485 1102 70-71 0.4656640547680482 1102 72-73 0.371086432097087 1102 74-75 0.225274892018696 1102 76-77 0.4607027006145863 1102 78-79 0.41747642689101383 1102 80-81 0.5612717397846936 1102 82-83 0.6340639207167662 1102 84-85 0.7417775134051894 1102 86-87 0.5580661818453514 1102 88-89 0.6271274157424749 1102 90-91 0.6249013082733441 1102 92-93 0.5751945047563787 1102 94-95 0.7014961894222509 1102 96-97 0.5012793576015611 1102 98-99 0.729275141680052 1102 100-101 0.7732880095546619 1103 1 0.23411816562943244 1103 2 0.462022376783942 1103 3 0.3816397843921209 1103 4 0.43598471944977035 1103 5 0.43456900247741714 1103 6 0.3620256058342619 1103 7 0.3853992097589156 1103 8 0.33979100654575944 1103 9 0.5596861292865825 1103 10-11 0.42039393065488184 1103 12-13 0.5426310794121463 1103 14-15 0.37551802608999907 1103 16-17 0.5227062554465576 1103 18-19 0.5724588884248902 1103 20-21 0.47743524572056373 1103 22-23 0.5471024344908031 1103 24-25 0.5904355844330169 1103 26-27 0.42208364781843244 1103 28-29 0.032948023917917624 1103 30-31 0.138697563992217 1103 32-33 0.5042857935265559 1103 34-35 0.20546251099852242 1103 36-37 0.2832333393837274 1103 38-39 0.42691605007854605 1103 40-41 0.249347311541392 1103 42-43 0.2643337039663578 1103 44-45 0.23987465272397657 1103 46-47 0.41886804038365 1103 48-49 0.4237312191463758 1103 50-51 0.5180947583993429 1103 52-53 0.5029731094743255 1103 54-55 0.5514717988421936 1103 56-57 0.5713690494185144 1103 58-59 0.36276935686551326 1103 60-61 0.33234513353474426 1103 62-63 0.5179602742431868 1103 64-65 0.4898094479904387 1103 66-67 0.36746948346021213 1103 68-69 0.5229230851747602 1103 70-71 0.5752660748921201 1103 72-73 0.5415483339804439 1103 74-75 0.40803245569419033 1103 76-77 0.48616218023542856 1103 78-79 0.5334309912481743 1103 80-81 0.3244516345770414 1103 82-83 0.1978991125470948 1103 84-85 0.23486549083742148 1103 86-87 0.339484855988875 1103 88-89 0.5358156212532279 1103 90-91 0.6257426693705206 1103 92-93 0.4612752839960912 1103 94-95 0.4810788743180474 1103 96-97 0.5376733160929845 1103 98-99 0.35302381801004756 1103 100-101 0.9138049589615278 1104 1 0.2809797548707458 1104 2 0.31738626629378075 1104 3 0.3089281169113214 1104 4 0.42003255730774924 1104 5 0.3846937440939229 1104 6 0.22897087131036642 1104 7 0.33592480800811586 1104 8 0.4277262568155251 1104 9 0.48707519342087835 1104 10-11 0.5790013781709504 1104 12-13 0.5129238772396576 1104 14-15 0.5801263594923256 1104 16-17 0.4276742559101976 1104 18-19 0.6015875737531289 1104 20-21 0.7726747799817346 1104 22-23 0.4151988691435875 1104 24-25 0.5202121948279697 1104 26-27 0.2256573433440181 1104 28-29 0.5274229638239234 1104 30-31 0.45099420341995966 1104 32-33 0.24884525139728453 1104 34-35 0.1395871394644459 1104 36-37 0.30046603493415347 1104 38-39 0.2715589220058874 1104 40-41 0.3522594901316438 1104 42-43 0.41657298156889055 1104 44-45 0.45743872769038063 1104 46-47 0.4681289049092854 1104 48-49 0.39501649935964167 1104 50-51 0.3983190035331816 1104 52-53 0.4866763210190044 1104 54-55 0.5331803864193674 1104 56-57 0.4922698936322476 1104 58-59 0.4445717076378344 1104 60-61 0.4634814827450313 1104 62-63 0.6194411669736795 1104 64-65 0.4408759612138766 1104 66-67 0.5409486430549606 1104 68-69 0.3900580848573938 1104 70-71 0.5820250972710923 1104 72-73 0.5038967880951333 1104 74-75 0.22991619209197367 1104 76-77 0.4247959283622329 1104 78-79 0.42414201846226973 1104 80-81 0.4369433207690818 1104 82-83 0.35908280561648453 1104 84-85 0.4183615816228219 1104 86-87 0.5477226322763791 1104 88-89 0.4567509625954145 1104 90-91 0.5409625264696167 1104 92-93 0.45418508918390543 1104 94-95 0.3602350883547949 1104 96-97 0.557938654206243 1104 98-99 0.8544954655101087 1104 100-101 1.3041220289686208 1105 1 0.3246081544224282 1105 2 0.29672042306772184 1105 3 0.23399822372986634 1105 4 0.35358341620204925 1105 5 0.40226070582681217 1105 6 0.308421224703622 1105 7 0.44840176310773217 1105 8 0.4203361060801498 1105 9 0.47103640021884274 1105 10-11 0.4525611576718589 1105 12-13 0.46315272510900485 1105 14-15 0.1730247560704754 1105 16-17 0.28094583119783323 1105 18-19 0.3068136768204397 1105 20-21 0.3610560329838606 1105 22-23 0.1514143589763819 1105 24-25 0.34100875925076224 1105 26-27 0.3841478165936323 1105 28-29 0.21752406403206948 1105 30-31 0.15490497448059415 1105 32-33 0.18190147409222135 1105 34-35 0.13720179213262185 1105 36-37 0.1729423038332527 1105 38-39 0.21808377390094336 1105 40-41 -0.023946392894039548 1105 42-43 0.16257998081407266 1105 44-45 -0.02711112477651767 1105 46-47 0.1764064272679846 1105 48-49 0.3420276386812162 1105 50-51 0.3634592171702735 1105 52-53 0.20188995811042787 1105 54-55 0.1242237542914566 1105 56-57 0.4041125707291613 1105 58-59 0.36676251712570007 1105 60-61 0.056413045681061647 1105 62-63 0.043199029687833956 1105 64-65 0.16529394881652593 1105 66-67 0.3459675135689295 1105 68-69 0.3323582289594924 1105 70-71 0.4617580509068162 1105 72-73 0.31044565700796056 1105 74-75 0.4342699055803898 1105 76-77 0.19888270985311962 1105 78-79 0.24212115796683165 1105 80-81 -0.07572005213514643 1105 82-83 0.08392143413040287 1105 84-85 0.21032445817535006 1105 86-87 0.38303817014320884 1105 88-89 0.2958657639403697 1105 90-91 0.35183743211704765 1105 92-93 0.3477696862698423 1105 94-95 0.34193880984888025 1105 96-97 0.3785892866543783 1105 98-99 0.5983660938410402 1105 100-101 0.9365100067357233 1106 1 0.162481041618868 1106 2 0.12993468792305407 1106 3 0.27863675472544003 1106 4 0.21205904376457596 1106 5 0.20804210731497363 1106 6 0.26528744204058796 1106 7 0.4013485327230697 1106 8 0.4206951804765353 1106 9 0.5779609058198858 1106 10-11 0.484733476002873 1106 12-13 0.5539757694150964 1106 14-15 0.714150105040801 1106 16-17 0.8798346162554722 1106 18-19 0.8716686570211181 1106 20-21 0.6575308195133118 1106 22-23 0.5505688871217842 1106 24-25 0.4481606942108698 1106 26-27 0.24352451764142558 1106 28-29 0.5911841932240947 1106 30-31 0.5142579630682391 1106 32-33 0.3385612878968445 1106 34-35 0.27429029918211967 1106 36-37 0.2641834116607029 1106 38-39 0.3907721594678861 1106 40-41 0.2667259663205286 1106 42-43 0.10318517419327122 1106 44-45 0.44239570277666473 1106 46-47 0.44334202983313986 1106 48-49 0.47388667219405534 1106 50-51 0.3050863825383985 1106 52-53 0.41196366531410433 1106 54-55 0.4679995255304661 1106 56-57 0.27995389582348906 1106 58-59 0.4501023528906316 1106 60-61 0.4841367663179099 1106 62-63 0.6542770705480194 1106 64-65 0.4755577061317666 1106 66-67 0.4999166310682597 1106 68-69 0.5366490161430804 1106 70-71 0.6468364889215081 1106 72-73 0.6799031906784876 1106 74-75 0.5512781640578872 1106 76-77 0.6989288498617618 1106 78-79 0.8038403697319509 1106 80-81 0.7200453463626744 1106 82-83 0.8138427040903373 1106 84-85 0.8447180382895922 1106 86-87 0.9656169877908845 1106 88-89 0.9926399348803407 1106 90-91 1.0373694439382355 1106 92-93 1.0476581325780572 1106 94-95 1.0966634299238756 1106 96-97 1.2051770285973937 1106 98-99 1.539784869904306 1106 100-101 1.9866995896066832 1107 1 0.10334211583016994 1107 2 0.13507067603188716 1107 3 0.04024826524477376 1107 4 0.06868532145949757 1107 5 0.23095182385092272 1107 6 0.254885372430266 1107 7 0.18287153472039108 1107 8 0.31231365481865225 1107 9 0.35552753817010796 1107 10-11 0.3020551186367726 1107 12-13 0.38433938719980887 1107 14-15 0.3450342989636752 1107 16-17 0.36609423315069733 1107 18-19 0.5034755462090104 1107 20-21 0.5902538542815634 1107 22-23 0.5515966924462532 1107 24-25 0.43858284952077753 1107 26-27 0.306053074041408 1107 28-29 0.3301376211399756 1107 30-31 0.5294535690626176 1107 32-33 0.3407423778994172 1107 34-35 0.45319178427246953 1107 36-37 0.4615911320058217 1107 38-39 0.39378681500623713 1107 40-41 0.3505127509025243 1107 42-43 0.42292682402057125 1107 44-45 0.5121266615379554 1107 46-47 0.45443496738246125 1107 48-49 0.5825475160185078 1107 50-51 0.5369063936030898 1107 52-53 0.5891708977865022 1107 54-55 0.6590636174583224 1107 56-57 0.6140689524006078 1107 58-59 0.77581265606225 1107 60-61 0.8202624185292038 1107 62-63 0.8728376839406096 1107 64-65 0.9192458358074163 1107 66-67 0.7953119101635977 1107 68-69 0.8000848726730325 1107 70-71 0.8217424413019287 1107 72-73 0.7319391972884901 1107 74-75 0.6985954267308507 1107 76-77 0.6820160335918963 1107 78-79 0.6613173186682673 1107 80-81 0.8361917369912675 1107 82-83 0.9977445695575646 1107 84-85 0.8853656963159224 1107 86-87 0.8138017123057466 1107 88-89 0.7765678640411124 1107 90-91 0.6965579537656872 1107 92-93 0.6818069279087418 1107 94-95 0.747667534843071 1107 96-97 0.907567798538711 1107 98-99 1.2593196082162663 1107 100-101 1.6103559476849227 1108 1 -0.00421348967424251 1108 2 0.14352491589248473 1108 3 0.1541154529621025 1108 4 0.10465840248681246 1108 5 0.17412800002598772 1108 6 0.09338989620190574 1108 7 -0.05491472694511401 1108 8 -0.020758225915344042 1108 9 0.12189829494352722 1108 10-11 -0.007089175825093719 1108 12-13 0.10202287785349995 1108 14-15 0.09576174102701884 1108 16-17 0.15384566661492016 1108 18-19 0.03819241963798703 1108 20-21 0.0798667048863777 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2316 94-95 -4.335726976063345 2316 96-97 -5.2915434709663 2316 98-99 -6.393339132684705 2316 100-101 -6.790042161853563 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6373.0 3 2808.0 4 2819.0 5 3132.0 6 3472.0 7 4045.0 8 4864.0 9 6198.0 10 7640.0 11 9201.0 12 10879.0 13 12384.0 14 13631.0 15 15418.0 16 17779.0 17 20320.0 18 19118.0 19 16876.0 20 18040.0 21 19750.0 22 22043.0 23 25324.0 24 29502.0 25 34002.0 26 39904.0 27 47089.0 28 56064.0 29 66692.0 30 81027.0 31 98780.0 32 122264.0 33 156092.0 34 203785.0 35 273840.0 36 377126.0 37 476758.0 38 347657.0 39 45763.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.54965629921839 17.86592725613436 14.034215523205324 23.550200921441924 2 15.891412970689744 19.344842557638106 39.64173491989904 25.12200955177311 3 20.351403151097173 22.845252142596696 28.655218634455874 28.148126071850253 4 13.222921797083378 15.660605061303686 36.31814245392148 34.798330687691454 5 14.568483414589977 36.76218463207781 33.690050373476296 14.979281579855911 6 33.686175484776115 34.651035174170495 16.99597251312899 14.6668168279244 7 30.14450604313724 31.42596986657215 19.839465537794865 18.59005855249574 8 26.98884996417282 33.95647227984872 19.30744428754337 19.747233468435095 9 26.318650340344345 16.97217184445439 19.853616600325037 36.855561214876225 10-11 25.78559754549247 25.702986848723047 26.737065187366543 21.774350418417935 12-13 26.699715971039534 22.902855102906443 26.991584020403714 23.40584490565031 14-15 23.399837595814514 24.7553954862088 25.242939254468 26.601827663508686 16-17 23.257057186039713 27.70808478578951 25.880817530724205 23.154040497446573 18-19 23.140691305868884 26.837823050681642 27.43169175656131 22.589793886888163 20-21 23.996591012354546 25.831414413476427 27.206396064322657 22.965598509846366 22-23 23.541396807070004 25.651356210620275 27.345085644030565 23.46216133827916 24-25 23.590951363505265 25.818824201114822 27.42253580194593 23.167688633433986 26-27 23.298570603953216 25.881475611456505 27.54947223120508 23.2704815533852 28-29 23.029231606017568 26.615000293384757 27.27183265407252 23.083935446525157 30-31 23.379405019844995 25.861588486315256 27.132359879721495 23.626646614118247 32-33 23.220499754539006 25.91681842820721 27.277034691391428 23.585647125862355 34-35 23.933712405489484 25.979576310344726 26.949513945911583 23.137197338254207 36-37 23.781655974799385 25.722820813501645 27.22536616771286 23.27015704398611 38-39 23.368533591943972 26.04658486609008 27.272894500949096 23.311987041016852 40-41 23.62052582963049 25.982900292045823 27.099766812131655 23.296807066192027 42-43 23.431486586604926 25.996320632489233 26.744609516914718 23.827583263991126 44-45 23.573070688495452 25.93519677118715 26.864916972254065 23.62681556806333 46-47 24.007937602746857 26.228465233719323 26.565752654175 23.197844509358813 48-49 23.84651816809871 26.1455636658258 26.58852114223914 23.419397023836346 50-51 23.42414455243042 26.409584838474725 26.87817735041163 23.288093258683226 52-53 23.853669662334415 26.427767375302114 26.322833168406067 23.395729793957408 54-55 23.467218933039174 26.203700280345448 26.497455075474015 23.831625711141363 56-57 23.736172321666572 26.29560698662804 26.306589474067625 23.661631217637765 58-59 24.066953872868947 26.05139911851487 26.267557962430192 23.614089046185995 60-61 23.642735302779492 26.156382955044833 26.91828221599207 23.282599526183606 62-63 23.329968469113087 26.34903893518234 26.86817102094076 23.452821574763814 64-65 23.288617575895245 26.598055706018187 26.465113156585467 23.6482135615011 66-67 23.395186626739164 26.390687425043964 26.253982326929727 23.96014362128715 68-69 23.464520113268378 26.249577119978717 26.589499992549587 23.696402774203314 70-71 23.643952258467042 26.133096224704815 26.366569007734235 23.85638250909391 72-73 23.559322314472173 26.105305713895255 26.674684865120085 23.66068710651249 74-75 23.396238145224345 26.818992214625954 26.16370897743185 23.62106066271785 76-77 23.634411197088745 26.273085387145834 26.26743842977061 23.82506498599481 78-79 23.60016274781887 26.403571181569767 26.36488914460276 23.631376926008603 80-81 23.782633680180002 26.330915560421513 26.239936369750026 23.64651438964846 82-83 23.712911271052025 26.3851924855116 26.41396051312302 23.487935730313353 84-85 23.606786513067245 26.499137778474374 26.26479086115088 23.629284847307503 86-87 23.6347627244157 26.55071832521133 26.197082995964077 23.61743595440889 88-89 23.551044064962078 26.500320881464784 25.909784944120272 24.038850109452863 90-91 23.561289824176754 26.33877841562268 26.18522967014978 23.914702090050785 92-93 23.740355265096724 26.301522977293768 26.3991683586286 23.558953398980904 94-95 23.498516907257265 26.252075180155533 26.50248561744945 23.746922295137754 96-97 23.28499770894622 26.242324142078076 26.731511490310712 23.741166658664994 98-99 23.230117068048266 26.51318832255693 26.12079677064825 24.135897838746555 100-101 23.318207594418723 26.126343772436073 26.33367102824236 24.221777604902844 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 73.0 1 59.5 2 66.0 3 91.5 4 83.5 5 98.0 6 164.0 7 291.0 8 484.0 9 627.5 10 655.0 11 665.0 12 733.5 13 799.5 14 895.5 15 1031.0 16 1171.5 17 1305.5 18 1521.5 19 1771.0 20 1987.0 21 2373.5 22 2873.0 23 3535.5 24 4526.5 25 5841.5 26 7854.5 27 10506.5 28 13327.5 29 16997.0 30 21670.5 31 26909.0 32 33310.0 33 38006.5 34 42167.0 35 47553.0 36 53958.0 37 59883.5 38 63700.0 39 67428.0 40 71625.5 41 76481.5 42 79919.0 43 84208.0 44 89824.0 45 96050.0 46 101267.0 47 109328.0 48 122392.0 49 121851.0 50 110156.0 51 104101.5 52 103353.5 53 104292.0 54 104350.5 55 98727.5 56 89672.0 57 82183.5 58 76953.0 59 69683.5 60 58778.0 61 49256.0 62 41174.0 63 32428.5 64 23724.0 65 17835.0 66 14016.0 67 11172.0 68 8693.0 69 6686.5 70 5226.5 71 3888.0 72 2990.0 73 2573.0 74 2018.5 75 1425.0 76 1044.0 77 699.0 78 471.0 79 309.5 80 208.5 81 143.5 82 92.5 83 61.0 84 38.5 85 28.0 86 26.0 87 19.5 88 13.0 89 11.5 90 8.0 91 7.0 92 9.0 93 8.0 94 4.0 95 1.0 96 1.0 97 1.0 98 1.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.036380771676999074 2 0.023873731868930638 3 0.0228805257665252 4 0.052308854726686234 5 0.05749559770591462 6 0.051131721568279796 7 0.052235283904285834 8 0.046276047289853225 9 0.04491498707544578 10-11 0.055490792795503646 12-13 0.048427993845065 14-15 0.043204465454636416 16-17 0.05273188695548855 18-19 0.056594355131509684 20-21 0.013242748032072463 22-23 0.0228253476497249 24-25 0.006658159427236433 26-27 0.012065614873666022 28-29 0.007191547889639352 30-31 0.010207951608055857 32-33 0.004211929582423048 34-35 0.009306709033650926 36-37 0.010355093252856664 38-39 0.013040428270471357 40-41 0.0022071246720120775 42-43 0.002464622550413486 44-45 0.002593371489614191 46-47 5.885665792032207E-4 48-49 0.001342667508807347 50-51 3.310687008018116E-4 52-53 0.0036417557088199277 54-55 0.007927256113643378 56-57 0.018595025361701753 58-59 0.012102400284866225 60-61 0.012047222168065922 62-63 0.006694944838436635 64-65 5.517811680030194E-5 66-67 0.007688150940842069 68-69 0.018374312894500543 70-71 0.006069592848033213 72-73 0.0025565860784139897 74-75 0.003770504648020632 76-77 0.007026013539238446 78-79 0.005628167913630798 80-81 0.009748133968053341 82-83 0.006382268843234924 84-85 0.018024851488098632 86-87 0.00485567427842657 88-89 0.006455839665635326 90-91 0.004193536876822947 92-93 0.01730753596969471 94-95 0.012194363812866729 96-97 0.01057580572005787 98-99 0.023836946457730435 100-101 0.03774183189140652 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2718469.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.144586768038494 #Duplication Level Percentage of deduplicated Percentage of total 1 77.77345505928645 44.44331950885537 2 12.681104738770252 14.493129801184814 3 4.15285375388942 7.119393350223251 4 1.7829867678405817 4.075521682445225 5 0.9492033134546733 2.7120915553110114 6 0.5750141886996578 1.971536891940051 7 0.3879517297078467 1.5518538896070464 8 0.2732329560925814 1.2491027493856135 9 0.20851104520165786 1.072374976315848 >10 1.092265321777168 11.375571983952087 >50 0.08174731603759743 3.20437768734997 >100 0.037544255927740376 3.81727920085189 >500 0.0027100193625019396 1.0591518699797113 >1k 0.0013550096812215008 1.5693515018041004 >5k 6.452427053435719E-5 0.2859433507939194 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7755 0.285270863857561 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4558 0.16766790425051747 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3873 0.14246989757837958 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3375 0.12415076280067935 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3178 0.11690403679423969 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3068 0.11285764156221756 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 3.678541120020129E-5 8 0.0 0.0 0.0 0.0 3.678541120020129E-5 9 0.0 0.0 0.0 0.0 3.678541120020129E-5 10-11 0.0 0.0 0.0 0.0 3.678541120020129E-5 12-13 0.0 0.0 0.0 0.0 3.678541120020129E-5 14-15 0.0 0.0 0.0 0.0 7.357082240040258E-5 16-17 0.0 0.0 0.0 3.678541120020129E-5 1.1035623360060387E-4 18-19 0.0 0.0 0.0 1.1035623360060387E-4 1.4714164480080516E-4 20-21 0.0 3.678541120020129E-5 0.0 1.4714164480080516E-4 1.4714164480080516E-4 22-23 0.0 3.678541120020129E-5 0.0 1.8392705600100645E-4 1.655343504009058E-4 24-25 0.0 3.678541120020129E-5 0.0 2.5749787840140904E-4 2.023197616011071E-4 26-27 0.0 3.678541120020129E-5 0.0 2.5749787840140904E-4 2.2071246720120773E-4 28-29 0.0 5.517811680030194E-5 0.0 4.4142493440241546E-4 2.391051728013084E-4 30-31 0.0 7.357082240040258E-5 0.0 7.908863408043277E-4 2.5749787840140904E-4 32-33 0.0 7.357082240040258E-5 0.0 0.001397845625607649 2.5749787840140904E-4 34-35 0.0 7.357082240040258E-5 0.0 0.0019496267936106684 2.5749787840140904E-4 36-37 0.0 1.1035623360060388E-4 0.0 0.002758905840015097 2.5749787840140904E-4 38-39 0.0 1.4714164480080516E-4 0.0 0.003844075470421035 2.5749787840140904E-4 40-41 0.0 1.4714164480080516E-4 0.0 0.005517811680030194 2.5749787840140904E-4 42-43 0.0 1.4714164480080516E-4 0.0 0.007890470702443177 2.758905840015097E-4 44-45 0.0 1.4714164480080516E-4 0.0 0.013757743788875282 2.942832896016103E-4 46-47 0.0 1.8392705600100645E-4 0.0 0.027552272988950764 2.942832896016103E-4 48-49 0.0 2.2071246720120773E-4 0.0 0.0368589820226017 3.678541120020129E-4 50-51 0.0 2.2071246720120773E-4 0.0 0.04835442302266459 3.678541120020129E-4 52-53 0.0 2.2071246720120773E-4 0.0 0.06328929996994631 3.678541120020129E-4 54-55 0.0 3.1267599520171097E-4 0.0 0.08219700132684979 3.678541120020129E-4 56-57 0.0 4.046395232022142E-4 0.0 0.10321986382776482 3.678541120020129E-4 58-59 0.0 4.4142493440241546E-4 0.0 0.13056981705511447 3.8624681760211354E-4 60-61 0.0 4.4142493440241546E-4 0.0 0.16606773886330872 4.230322288023148E-4 62-63 0.0 4.4142493440241546E-4 0.0 0.21035737394835108 4.4142493440241546E-4 64-65 0.0 4.4142493440241546E-4 0.0 0.24127551206212028 4.4142493440241546E-4 66-67 0.0 4.4142493440241546E-4 0.0 0.29783308178242973 4.4142493440241546E-4 68-69 0.0 4.598176400025161E-4 0.0 0.36998766585162457 4.4142493440241546E-4 70-71 0.0 5.149957568028181E-4 0.0 0.41611657149667697 4.4142493440241546E-4 72-73 0.0 5.517811680030194E-4 0.0 0.4991044591643311 4.7821034560261675E-4 74-75 0.0 5.517811680030194E-4 0.0 0.5666424741279006 4.7821034560261675E-4 76-77 0.0 5.517811680030194E-4 0.0 0.6240277156002147 4.7821034560261675E-4 78-79 0.0 5.517811680030194E-4 0.0 0.7041831266054532 4.7821034560261675E-4 80-81 0.0 5.885665792032207E-4 0.0 0.785442099946698 4.966030512027174E-4 82-83 0.0 5.885665792032207E-4 0.0 0.8889930324752646 5.885665792032207E-4 84-85 0.0 5.885665792032207E-4 0.0 1.0525041852601593 5.885665792032207E-4 86-87 0.0 5.885665792032207E-4 0.0 1.2772630476933893 5.885665792032207E-4 88-89 0.0 5.885665792032207E-4 0.0 1.5495854468084793 5.885665792032207E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTCCG 315 0.0 24.137814 5 GTACATG 10315 0.0 23.910824 1 TACATGG 10135 0.0 23.632586 2 ACATGGG 10135 0.0 22.272774 3 GTGTAGC 1665 0.0 20.264717 1 GTGTACG 340 4.1836756E-11 19.56796 1 GAGTACT 5590 0.0 18.406641 12-13 ACCGGTC 160 0.0011881489 17.820166 8 GTATCAA 15980 0.0 17.813387 1 CATGGGG 6220 0.0 17.650215 4 AGAGTAC 11150 0.0 17.64501 10-11 GTACTTT 5795 0.0 17.590181 14-15 GTATAGT 720 0.0 17.160791 1 AGTACTT 5770 0.0 17.091127 12-13 GTATAGG 625 0.0 16.727812 1 TAGCCCT 1925 0.0 16.54141 4 GTATAGC 875 0.0 16.293322 1 GTGTAAG 1415 0.0 15.784755 1 TCAACGC 17740 0.0 15.752597 4 CATGGGA 4175 0.0 15.709091 4 >>END_MODULE