FastQCFastQC Report
Thu 26 May 2016
SRR936457_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936457_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2718469
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT85690.3152141885745249No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46400.17068430796893397No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT42080.15479301033044704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41380.15221803154643293No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT34170.1256957500710878No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC31250.11495441000062903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT10400.021.921144
GAGTACT70550.021.57795312-13
TCGCGTA1604.99519E-520.7797999
TAAGGTG12450.020.6005925
GTACTTT74050.020.36563314-15
TACATGG94050.020.3023592
GTACATG99000.019.8717521
TACACTG22800.019.1649745
GTACACT14250.018.6650184
AGTACTT76200.018.63783512-13
ACATGGG96150.018.62293
TCTATAC8200.018.534953
GTGTAGC17150.018.2873521
AGAGTAC125700.018.21339810-11
CCTATAC6400.017.8109283
ACTTTTT85500.017.66608216-17
GTATCAA186000.017.1427521
GTGTAAG13650.017.0582491
GAGTACA84600.016.2329881
AGTACAT78500.016.2160242
Produced by FastQC (version 0.11.5)