##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936457_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2718469 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.89388917070601 31.0 31.0 34.0 30.0 34.0 2 32.04288001812785 31.0 31.0 34.0 30.0 34.0 3 32.16452459086346 33.0 31.0 34.0 30.0 34.0 4 35.89311594136258 37.0 35.0 37.0 35.0 37.0 5 35.663143114745836 37.0 35.0 37.0 33.0 37.0 6 35.75187430866418 37.0 35.0 37.0 33.0 37.0 7 35.697739426125516 37.0 35.0 37.0 33.0 37.0 8 35.66698020098813 37.0 35.0 37.0 33.0 37.0 9 37.319647934186484 39.0 37.0 39.0 34.0 39.0 10-11 37.18682648211181 39.0 37.0 39.0 33.0 39.0 12-13 37.09330545980109 39.0 37.0 39.0 33.0 39.0 14-15 38.22974163030736 40.0 38.0 41.0 33.0 41.0 16-17 38.14533824001671 40.0 37.0 41.0 33.0 41.0 18-19 38.26397707680316 40.0 38.0 41.0 33.5 41.0 20-21 38.283968476374014 40.0 38.0 41.0 34.0 41.0 22-23 38.189716711869806 40.0 38.0 41.0 33.0 41.0 24-25 38.07091932996109 40.0 37.0 41.0 33.0 41.0 26-27 37.85805503023944 40.0 37.0 41.0 33.0 41.0 28-29 37.79740397996078 40.0 37.0 41.0 32.5 41.0 30-31 37.81474940490401 40.0 37.0 41.0 33.0 41.0 32-33 37.70504041061348 40.0 37.0 41.0 33.0 41.0 34-35 37.56014764192639 40.0 37.0 41.0 32.0 41.0 36-37 37.32493510133829 39.5 36.0 41.0 31.5 41.0 38-39 37.16785679733703 39.0 36.0 41.0 31.0 41.0 40-41 36.988887679057584 39.0 36.0 41.0 31.0 41.0 42-43 36.77470609376087 39.0 35.5 41.0 30.5 41.0 44-45 36.580620746456916 39.0 35.0 40.0 30.0 41.0 46-47 36.309541142459224 39.0 35.0 40.0 30.0 41.0 48-49 36.01173252297525 39.0 35.0 40.0 29.5 41.0 50-51 35.7185040550398 38.0 35.0 40.0 28.5 41.0 52-53 35.452261548687886 38.0 34.0 40.0 28.0 41.0 54-55 35.18696387562264 38.0 34.0 40.0 27.0 41.0 56-57 34.852946640186076 38.0 34.0 40.0 26.5 41.0 58-59 34.506720694626274 37.0 33.5 40.0 26.0 41.0 60-61 34.13444847081206 37.0 33.0 39.5 26.0 41.0 62-63 33.725329404160945 36.0 32.5 39.0 25.0 40.5 64-65 33.30542926919527 36.0 32.0 39.0 24.5 40.0 66-67 32.83571120362233 35.0 31.5 38.5 23.0 40.0 68-69 32.52994111759229 35.0 31.0 38.0 22.5 40.0 70-71 32.0861232921913 35.0 31.0 37.5 22.0 39.5 72-73 31.636284982466233 34.5 31.0 37.0 20.5 39.0 74-75 31.115552356859688 34.0 30.0 36.0 19.5 39.0 76-77 29.677188704377354 32.5 28.5 35.0 18.5 37.0 78-79 30.480075365950466 34.0 30.0 35.0 19.0 37.0 80-81 30.295585861012206 34.0 30.0 35.0 18.0 37.0 82-83 29.96588226682004 34.0 29.5 35.0 16.5 36.5 84-85 29.560745772712508 34.0 29.0 35.0 12.0 36.0 86-87 29.191047424119972 34.0 29.0 35.0 7.0 36.0 88-89 28.796179761476036 33.0 29.0 35.0 4.5 35.0 90-91 28.323201772762538 33.0 28.0 35.0 2.0 35.0 92-93 27.82775359954445 33.0 27.0 35.0 2.0 35.0 94-95 27.229033327214694 32.5 26.0 34.0 2.0 35.0 96-97 26.457112256935797 32.0 25.0 34.0 2.0 35.0 98-99 25.491931487907348 32.0 23.0 34.0 2.0 35.0 100-101 22.210373743456337 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2591547821202447 1101 2 0.382302357221846 1101 3 0.30224777392561464 1101 4 0.14859879908451035 1101 5 0.0865452034829417 1101 6 0.18552471415571858 1101 7 0.1506087030461103 1101 8 0.20583525277248782 1101 9 0.2961592181953563 1101 10-11 0.31478592170840614 1101 12-13 0.28947780445872695 1101 14-15 0.3847590208108187 1101 16-17 0.48112367675815193 1101 18-19 0.4158441764488572 1101 20-21 0.27220784436634915 1101 22-23 0.2474139817515848 1101 24-25 0.16150882875372474 1101 26-27 0.11250315948313272 1101 28-29 0.11080471545883341 1101 30-31 0.2368607934070326 1101 32-33 0.2364870679113409 1101 34-35 0.08784184614335544 1101 36-37 0.08247539938430037 1101 38-39 0.14008293305452213 1101 40-41 0.0907567639633271 1101 42-43 0.20423950216962083 1101 44-45 0.16016392668588963 1101 46-47 0.09313694095794034 1101 48-49 0.15293471939017422 1101 50-51 0.13487472831970848 1101 52-53 0.11579321422069455 1101 54-55 0.06622432791925092 1101 56-57 -0.07790905147894023 1101 58-59 0.08286772046765378 1101 60-61 0.0783806168095893 1101 62-63 0.1281255728290276 1101 64-65 0.03344629756482931 1101 66-67 -0.008509707042229309 1101 68-69 -0.14207974267813483 1101 70-71 -0.1518425419514955 1101 72-73 -0.14250134325509478 1101 74-75 -0.14442241458084837 1101 76-77 -0.07314957074756379 1101 78-79 -0.34124099800797936 1101 80-81 -0.0686165598971904 1101 82-83 0.20238098561177154 1101 84-85 0.08250061904694661 1101 86-87 -0.025593751853971725 1101 88-89 -0.24153776393823634 1101 90-91 -0.18193140096585125 1101 92-93 -0.17186902325424214 1101 94-95 -0.10081151554380696 1101 96-97 -0.22843222756116077 1101 98-99 -0.02855145617322208 1101 100-101 0.37590990534980406 1102 1 0.30994648500582755 1102 2 0.31186421110897555 1102 3 0.2656095209356124 1102 4 0.21257443791599684 1102 5 0.13542344476257995 1102 6 0.24875141098058862 1102 7 0.2751386090924868 1102 8 0.2826018084513606 1102 9 0.3696064167812878 1102 10-11 0.3577954454033545 1102 12-13 0.31558061404398075 1102 14-15 0.4964847255632421 1102 16-17 0.38548318095253364 1102 18-19 0.4220496422216087 1102 20-21 0.2700639101449127 1102 22-23 0.2783995774010961 1102 24-25 0.36668284497780235 1102 26-27 0.14648978680779123 1102 28-29 0.23605549071908882 1102 30-31 0.2742414715053414 1102 32-33 0.3178828772449478 1102 34-35 0.32882935174537664 1102 36-37 0.36703664168558703 1102 38-39 0.39704824410608097 1102 40-41 0.46562667664691304 1102 42-43 0.5751926336395243 1102 44-45 0.6584556220519673 1102 46-47 0.5654777205995529 1102 48-49 0.5154692714837523 1102 50-51 0.43007706297346004 1102 52-53 0.42960818644367293 1102 54-55 0.5755887348263613 1102 56-57 0.46381576597271135 1102 58-59 0.2777378938271724 1102 60-61 0.44294230025476367 1102 62-63 0.3573319048479604 1102 64-65 0.6091226513501979 1102 66-67 0.5155749945891088 1102 68-69 0.4278455736671489 1102 70-71 0.42851269417578663 1102 72-73 0.3910787298745326 1102 74-75 0.31014690867435135 1102 76-77 0.04195878020646049 1102 78-79 0.18618825103478898 1102 80-81 0.6066274728798007 1102 82-83 0.5223297377260074 1102 84-85 0.5414029277333725 1102 86-87 0.47488035249861227 1102 88-89 0.43249475237656654 1102 90-91 0.4298439324737586 1102 92-93 0.5382781383357624 1102 94-95 0.6022437567773196 1102 96-97 0.8578447386121297 1102 98-99 0.7983347848497537 1102 100-101 1.2172369664191578 1103 1 0.15965688530078737 1103 2 0.17531291273339633 1103 3 0.22598730814520707 1103 4 0.17699075839205136 1103 5 -0.053776694142385395 1103 6 0.16023593636718658 1103 7 0.1306795882010121 1103 8 0.15749730818770047 1103 9 0.17914370033177107 1103 10-11 0.12723463803966695 1103 12-13 0.2162732096376061 1103 14-15 0.1996128739686256 1103 16-17 0.3256511530686481 1103 18-19 0.26737960582336484 1103 20-21 0.27233290561334655 1103 22-23 0.15702171730927006 1103 24-25 0.1396696875907537 1103 26-27 0.24407052531919504 1103 28-29 0.2037793785147457 1103 30-31 0.16412820393750138 1103 32-33 0.24664477955702324 1103 34-35 0.24401693775713085 1103 36-37 0.24305538528160042 1103 38-39 0.3709939168305425 1103 40-41 0.2838155102310438 1103 42-43 0.2275197205365842 1103 44-45 0.2352306260176249 1103 46-47 0.23305541520583262 1103 48-49 0.2524565771132643 1103 50-51 0.40155652947549925 1103 52-53 0.3332171432416544 1103 54-55 0.35051445925434876 1103 56-57 0.4275064794897929 1103 58-59 0.4884983127714051 1103 60-61 0.46784444513607326 1103 62-63 0.4587936223821387 1103 64-65 0.18660182632329025 1103 66-67 0.52403898004696 1103 68-69 0.5022690537510073 1103 70-71 0.48170172068348194 1103 72-73 0.37120331562476494 1103 74-75 0.25482822038309294 1103 76-77 0.2026212368522451 1103 78-79 0.13840751509570381 1103 80-81 0.40686691245828754 1103 82-83 0.570558485051869 1103 84-85 0.36215871651208076 1103 86-87 0.18505449816876407 1103 88-89 0.16774038694504156 1103 90-91 0.0921365932326772 1103 92-93 0.25527320377972273 1103 94-95 0.3051468276917326 1103 96-97 0.38978109098487224 1103 98-99 0.6485339248393771 1103 100-101 0.9372512856347761 1104 1 0.17850404646308604 1104 2 0.20331423190358322 1104 3 0.13143619857957134 1104 4 0.05268794535820831 1104 5 0.042870460948421396 1104 6 0.12223389965672027 1104 7 0.10126526664913627 1104 8 0.0832663871695658 1104 9 0.15199790994736162 1104 10-11 0.20299585167624912 1104 12-13 0.11543568835287488 1104 14-15 0.1323407137032433 1104 16-17 0.24782287149480453 1104 18-19 0.12253513267731364 1104 20-21 0.1380259253263958 1104 22-23 0.1426150265222148 1104 24-25 0.1322597043977538 1104 26-27 0.1330263386372863 1104 28-29 0.10625151173437786 1104 30-31 0.02146567416648537 1104 32-33 0.11533525476186668 1104 34-35 0.10610742234519677 1104 36-37 0.19143112248523408 1104 38-39 0.31843719665193504 1104 40-41 0.39060482108881445 1104 42-43 0.2934019914963457 1104 44-45 0.23890388025593978 1104 46-47 0.2963717897508218 1104 48-49 0.22758276760917084 1104 50-51 0.2345207435343184 1104 52-53 0.2574086685390853 1104 54-55 0.23323733140247072 1104 56-57 0.1798657381051072 1104 58-59 0.025131848395908207 1104 60-61 0.2909818071820567 1104 62-63 0.15802937299825714 1104 64-65 0.34200069592138505 1104 66-67 0.13519562736762936 1104 68-69 0.38441030431331313 1104 70-71 0.1046812238696404 1104 72-73 0.10100490194072265 1104 74-75 0.2928930418673552 1104 76-77 0.19596018631809287 1104 78-79 0.18761628895736493 1104 80-81 0.3797618286854565 1104 82-83 0.26228007114704255 1104 84-85 0.18240487422191265 1104 86-87 0.17147746332114977 1104 88-89 0.04470465343949925 1104 90-91 0.1488422830510565 1104 92-93 0.27868468000843905 1104 94-95 0.21453287539712207 1104 96-97 0.38290719316812627 1104 98-99 0.37900372053358566 1104 100-101 0.9960795837672372 1105 1 0.03045697310825446 1105 2 0.15913138234161295 1105 3 0.09224728757977374 1105 4 0.06333045776699464 1105 5 -0.04899257343021901 1105 6 0.045069737621908246 1105 7 0.15053331944082515 1105 8 0.046194176002799736 1105 9 -0.07585473993026426 1105 10-11 -0.012698697604385245 1105 12-13 -0.11551344131614627 1105 14-15 0.03837767703726058 1105 16-17 0.13147117086121085 1105 18-19 0.12154791888010408 1105 20-21 0.12788799902433112 1105 22-23 0.10397965059869563 1105 24-25 0.03603792439363929 1105 26-27 0.13087098284233178 1105 28-29 0.11316738355211697 1105 30-31 0.17296856764529167 1105 32-33 0.12077785408938979 1105 34-35 0.08556246477525775 1105 36-37 -0.1018850558354032 1105 38-39 0.12923742081076028 1105 40-41 0.13533388625498333 1105 42-43 0.12137735689811535 1105 44-45 0.1417074667972571 1105 46-47 0.1808601129055134 1105 48-49 0.09961346364521262 1105 50-51 0.0793813304644928 1105 52-53 0.08164492737391527 1105 54-55 0.07319524443798997 1105 56-57 0.14173117418552295 1105 58-59 0.11156394885566101 1105 60-61 0.0667536683865606 1105 62-63 0.19407674370664552 1105 64-65 0.14492539685233652 1105 66-67 0.3855247748778723 1105 68-69 0.3892370386850885 1105 70-71 0.2879523416902643 1105 72-73 0.27113676680496823 1105 74-75 -0.0726160045426738 1105 76-77 -0.13051880450969477 1105 78-79 0.07944341217610429 1105 80-81 0.36690723457305907 1105 82-83 0.27236870537929647 1105 84-85 0.24383503044689547 1105 86-87 0.20488639307708922 1105 88-89 0.2169511595352489 1105 90-91 0.3457321936162572 1105 92-93 0.40691462579859916 1105 94-95 0.30706461217060976 1105 96-97 0.4057812296282499 1105 98-99 0.6488367450766894 1105 100-101 0.7720860111786614 1106 1 0.13080763717394817 1106 2 0.18788046246169188 1106 3 0.1346937593292381 1106 4 0.07967710356358282 1106 5 0.014374128668855235 1106 6 -0.017062281207891772 1106 7 0.14338220215175568 1106 8 0.03747266307775021 1106 9 0.07903715227732988 1106 10-11 0.17809841484874767 1106 12-13 0.18682498104598722 1106 14-15 0.2081544528435444 1106 16-17 0.1354932947033305 1106 18-19 0.15432204309601616 1106 20-21 0.12010166814789613 1106 22-23 0.14011895838175548 1106 24-25 0.03213261069998197 1106 26-27 0.17648861650233982 1106 28-29 0.10061286241532486 1106 30-31 0.2391943149382172 1106 32-33 0.1290068954285033 1106 34-35 0.18625725153832917 1106 36-37 0.07253457294194021 1106 38-39 0.3468255248652454 1106 40-41 0.3873791151444692 1106 42-43 0.28532121794076915 1106 44-45 0.3152742646806601 1106 46-47 0.35795282512034277 1106 48-49 0.22860212137980085 1106 50-51 0.27951437722359174 1106 52-53 0.139135845384871 1106 54-55 0.10850321439441757 1106 56-57 0.1640930753875054 1106 58-59 0.16558747441956 1106 60-61 0.19576849159440712 1106 62-63 0.11414496083335024 1106 64-65 0.2165435678390324 1106 66-67 0.27997757773737675 1106 68-69 0.35267873289948426 1106 70-71 0.334086743447088 1106 72-73 0.1573482632597809 1106 74-75 0.20914280586277556 1106 76-77 0.025166462226280828 1106 78-79 0.24036761407076668 1106 80-81 0.34648169520219696 1106 82-83 0.5400001459988601 1106 84-85 0.27277544559015965 1106 86-87 0.32840109363427317 1106 88-89 0.38591972592429613 1106 90-91 0.5501951589586866 1106 92-93 0.5586211052575365 1106 94-95 0.8224400983928533 1106 96-97 0.8839572391599582 1106 98-99 1.0862366679819893 1106 100-101 1.3748719332195662 1107 1 -0.043190249821257964 1107 2 0.09501111088947312 1107 3 0.041381009861652274 1107 4 0.04525232117742206 1107 5 -0.017556821899702868 1107 6 0.03877209234740775 1107 7 0.010349103961857509 1107 8 -0.03576001368158188 1107 9 0.10175532992548142 1107 10-11 0.08463936658319682 1107 12-13 0.04721215068641982 1107 14-15 0.17115087279942287 1107 16-17 0.09389165597674065 1107 18-19 -0.04128395128340401 1107 20-21 0.05926961511661233 1107 22-23 -0.02726723578330592 1107 24-25 0.018601885026434672 1107 26-27 -0.011647698865651535 1107 28-29 -0.03722097456100215 1107 30-31 -0.04908780406257307 1107 32-33 0.10965606595933508 1107 34-35 -0.016768680261215252 1107 36-37 0.11745303049345779 1107 38-39 0.025973242086323012 1107 40-41 0.06609300016081932 1107 42-43 -0.19571992083107403 1107 44-45 0.03449693640281026 1107 46-47 0.1041170957128017 1107 48-49 -0.02990706685993416 1107 50-51 0.07992534180082345 1107 52-53 -0.05986067990613719 1107 54-55 0.07872038033011819 1107 56-57 -0.0605069774601219 1107 58-59 0.0459277744266231 1107 60-61 0.035531469535690974 1107 62-63 0.12426771227709565 1107 64-65 0.1514732386307216 1107 66-67 0.1822900440605224 1107 68-69 0.3115258938700265 1107 70-71 0.15218331956005215 1107 72-73 0.1056675376537548 1107 74-75 0.08853700279716392 1107 76-77 0.04141778285427833 1107 78-79 0.2313033906990576 1107 80-81 0.5322593363343984 1107 82-83 0.412074226308345 1107 84-85 0.38112050908609163 1107 86-87 0.4587425190167096 1107 88-89 0.5310239379342114 1107 90-91 0.5791895550394983 1107 92-93 0.33356763867375605 1107 94-95 0.6161395493447195 1107 96-97 0.7697952229194378 1107 98-99 0.9506719253620908 1107 100-101 1.1415620377039168 1108 1 0.08024590274184007 1108 2 0.024977829371898963 1108 3 -0.04467992057429626 1108 4 -0.10106072982864589 1108 5 -0.03013714652524868 1108 6 0.004555776948556911 1108 7 0.01114314244200898 1108 8 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2316 54-55 -0.9879866850586936 2316 56-57 -0.961578743968758 2316 58-59 -0.9661894828643867 2316 60-61 -0.7813426131111498 2316 62-63 -0.7557002750469834 2316 64-65 -0.999343469552251 2316 66-67 -1.8053445942012054 2316 68-69 -1.3017444654716392 2316 70-71 -0.763107141457251 2316 72-73 -0.6039943796795342 2316 74-75 -0.5534421951723054 2316 76-77 -0.4521318740463336 2316 78-79 -0.6929848922641888 2316 80-81 -1.2939924238660723 2316 82-83 -0.9106060964509517 2316 84-85 -0.7942889625929972 2316 86-87 -1.057750714611256 2316 88-89 -1.0310291201579922 2316 90-91 -1.321184091748183 2316 92-93 -1.131039231261397 2316 94-95 -1.3332531038827504 2316 96-97 -1.2472180614718376 2316 98-99 -1.319516673754272 2316 100-101 -1.5319450515267583 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 12.0 4 41.0 5 73.0 6 163.0 7 389.0 8 925.0 9 1797.0 10 3022.0 11 4476.0 12 6333.0 13 8174.0 14 10513.0 15 13549.0 16 16219.0 17 20428.0 18 18827.0 19 15128.0 20 14847.0 21 15786.0 22 17544.0 23 19936.0 24 23370.0 25 28836.0 26 35455.0 27 44520.0 28 54659.0 29 68242.0 30 84259.0 31 104681.0 32 129896.0 33 165947.0 34 217712.0 35 294654.0 36 417210.0 37 532507.0 38 311849.0 39 16487.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.0892866868526 18.636573729927534 14.660094708037525 23.614044875182344 2 16.087082399691866 19.874929137670286 38.8391784715368 25.19880999110105 3 20.35381562078676 22.82721415262483 29.041868917529474 27.77710130905894 4 13.64780232816923 16.17498238882891 35.91601080764922 34.261204475352635 5 14.488177274484624 36.77918049654013 33.83739489878611 14.89524733018914 6 33.883570323693704 34.850329137951015 16.642553794144856 14.623546744210428 7 30.03160970384433 31.55533500657907 19.72555140411754 18.68750388545906 8 27.116181939172378 33.953890958477 19.114361797026195 19.81556530532443 9 26.188113130088418 16.68559917717151 19.73975400488812 37.386533687851944 10-11 25.841561650023653 25.72362714450619 26.63200976435906 21.8028014411111 12-13 26.683833422385877 22.82727270219143 27.049135853106527 23.43975802231617 14-15 23.275224653216657 24.677532324018582 25.289268146255978 26.757974876508783 16-17 23.171595965771633 27.58091788572531 26.095331848262305 23.15215430024075 18-19 23.044036919310095 26.632067535072128 27.750859031315052 22.573036514302718 20-21 23.88859317505552 25.57211430404393 27.661176198808963 22.87811632209159 22-23 23.390154911591157 25.45764150261466 27.74606513375501 23.406138452039173 24-25 23.554749539948812 25.59609850843174 27.80467527881263 23.044476672806812 26-27 23.227868660081054 25.762327970155436 27.891320877988164 23.118482491775342 28-29 22.98327769532271 26.335077734722113 27.714608550773445 22.967036019181734 30-31 23.247640237981948 25.66100833878529 27.547217771653354 23.544133651579408 32-33 23.1990666769421 25.6373051124378 27.718043148350436 23.445585062269664 34-35 23.820628733130555 25.687414742251203 27.476208765416292 23.01574775920195 36-37 23.761948970512737 25.495576349338993 27.616982506819376 23.12549217332889 38-39 23.32559610645625 25.79925234806963 27.669872106748343 23.20527943872578 40-41 23.557091172613454 25.7991513437058 27.432658368939254 23.211099114741497 42-43 23.391488355967226 25.78345584482348 27.11756045029295 23.70749534891634 44-45 23.53523366552177 25.678763201060022 27.24545391340686 23.540549220011354 46-47 23.98171028345934 26.102758263617275 26.859544945819657 23.055986507103725 48-49 23.85366745486034 25.987271432418158 26.822765309080314 23.336295803641192 50-51 23.488591438990216 26.17301607796528 27.128710904522112 23.209681578522396 52-53 23.92685035314891 26.27707474985285 26.545357563272514 23.25071733372572 54-55 23.504160079605725 26.081248235403393 26.70214712896109 23.712444556029794 56-57 23.763040387841585 26.175076454989917 26.536829445089527 23.52505371207898 58-59 24.162626359235446 25.897095392945957 26.497218993246126 23.443059254572475 60-61 23.617517390531024 26.083469766622287 27.16608883697036 23.132924005876333 62-63 23.365620194677632 26.13048772412575 27.100693805778526 23.403198275418085 64-65 23.379218398957267 26.36120196847888 26.79057890746258 23.469000725101267 66-67 23.474414753182316 26.202875017979437 26.45274782839651 23.869962400441732 68-69 23.57636993992554 26.015785524926365 26.760071682491372 23.647772852656722 70-71 23.699096531077355 25.967581355150042 26.617598561541953 23.715723552230642 72-73 23.62331635665374 25.895616411648614 26.889331545539992 23.591735686157662 74-75 23.481849887605065 26.560556874079964 26.36440605794064 23.593187180374322 76-77 23.715839516656946 26.097718125051845 26.4494323040332 23.737010054258008 78-79 23.627597694502445 26.218063605716953 26.551976697817665 23.60236200196294 80-81 23.86936951767798 26.092785687733265 26.436304221750078 23.601540572838676 82-83 23.752009031952852 26.180799448488774 26.560404863393185 23.506786656165193 84-85 23.632383590902293 26.26281874538205 26.47040916797001 23.634388495745647 86-87 23.55412913357556 26.344394950078705 26.33598920533156 23.765486711014177 88-89 23.50304305415325 26.39852007153895 26.058097529669478 24.040339344638323 90-91 23.60494068790551 26.097946996575626 26.30648849738279 23.990623818136065 92-93 23.707027918437674 26.03755872171375 26.607114052507757 23.64829930734082 94-95 23.43501405784482 26.002304287320793 26.719175282913437 23.84350637192095 96-97 23.278022761236926 25.91799517185883 26.90821473732613 23.895767329578113 98-99 23.14964021816642 26.205849910433905 26.33655258722693 24.307957284172748 100-101 23.05495843165415 25.949687577540015 26.476348099558024 24.51900589124781 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 120.0 1 86.5 2 74.5 3 104.5 4 105.5 5 104.5 6 162.5 7 312.5 8 496.0 9 638.0 10 666.5 11 678.5 12 776.0 13 899.0 14 1043.0 15 1205.5 16 1433.0 17 1661.5 18 1848.0 19 2042.5 20 2347.0 21 2759.0 22 3231.0 23 4076.0 24 5063.0 25 6310.5 26 8378.0 27 11135.0 28 14022.0 29 17721.5 30 22370.0 31 27289.5 32 33366.0 33 38082.0 34 42032.5 35 47012.5 36 53576.0 37 59626.5 38 63302.0 39 67134.0 40 71365.5 41 75800.5 42 79391.0 43 83670.0 44 88989.0 45 94600.0 46 99881.5 47 108659.5 48 122670.0 49 122581.0 50 109575.0 51 103165.0 52 102880.0 53 104242.5 54 104499.5 55 99180.5 56 89884.5 57 82090.5 58 77187.5 59 70029.5 60 59259.5 61 49394.5 62 40921.0 63 32243.5 64 23428.0 65 17418.5 66 13921.0 67 11241.0 68 8684.0 69 6749.5 70 5293.5 71 3802.0 72 2945.5 73 2596.0 74 2066.0 75 1532.0 76 1129.0 77 721.5 78 487.0 79 357.5 80 242.5 81 164.5 82 113.0 83 76.5 84 46.0 85 23.5 86 11.5 87 8.5 88 6.0 89 4.0 90 4.0 91 2.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03917646292821437 2 0.006253519904034219 3 0.003825682764820934 4 8.82849868804831E-4 5 6.621374016036232E-4 6 1.4714164480080516E-4 7 0.0 8 0.0 9 0.0015082018592082528 10-11 4.046395232022142E-4 12-13 0.001342667508807347 14-15 0.0020967684384114735 16-17 0.002961225601616204 18-19 0.0 20-21 0.0 22-23 0.0017473070320095613 24-25 7.908863408043278E-4 26-27 0.004340678521623752 28-29 0.005646560619230898 30-31 0.0010115988080055356 32-33 3.8624681760211354E-4 34-35 0.008865284099248512 36-37 0.010870089009659481 38-39 0.008295110225645391 40-41 0.0019864122048108696 42-43 6.069592848033212E-4 44-45 0.0011771331584064413 46-47 5.517811680030194E-5 48-49 6.253519904034219E-4 50-51 0.0010299915136056362 52-53 0.002538193372813889 54-55 0.002630156900814392 56-57 0.001453023742407951 58-59 0.0010667769248058373 60-61 0.003954431704021638 62-63 0.004745318044825966 64-65 0.008387073753645894 66-67 0.003163545363217311 68-69 0.0034026505360186194 70-71 9.196352800050322E-5 72-73 0.0012507039808068439 74-75 0.005609775208030697 76-77 0.0025198006672137886 78-79 0.003568184886419525 80-81 0.004763710750426068 82-83 0.004414249344024155 84-85 0.004984423217627275 86-87 0.0033106870080181158 88-89 0.008442251870446196 90-91 0.011219550416061394 92-93 0.001195525864006542 94-95 0.0021335538496116747 96-97 0.0011587404528063407 98-99 0.0031451526576172107 100-101 0.004211929582423048 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2718469.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.77772692288384 #Duplication Level Percentage of deduplicated Percentage of total 1 76.06293494017898 41.66554679106204 2 13.603998854294005 14.903922685994834 3 4.442387489452612 7.30031666348612 4 1.9497414691470083 4.272096230686287 5 1.0459843047111954 2.8648321304546194 6 0.6337510620542096 2.0829265568575863 7 0.4051032507501378 1.5533444671614514 8 0.29115431925906726 1.275901743423306 9 0.22282210922510193 1.0985119786362265 >10 1.2030826242164057 12.092900595221243 >50 0.09146277480357895 3.4452821285753124 >100 0.043073991367188966 4.221095595213467 >500 0.002957069608713095 1.1440683355725796 >1k 0.0014785348043472703 1.763795026075535 >5k 6.720612747033047E-5 0.31545907157941305 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8569 0.3152141885745249 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4640 0.17068430796893397 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4208 0.15479301033044704 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4138 0.15221803154643293 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3417 0.1256957500710878 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3125 0.11495441000062903 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.678541120020129E-5 0.0 12-13 0.0 0.0 0.0 3.678541120020129E-5 0.0 14-15 0.0 1.8392705600100645E-5 0.0 3.678541120020129E-5 5.517811680030193E-5 16-17 0.0 3.678541120020129E-5 0.0 7.357082240040258E-5 1.4714164480080516E-4 18-19 0.0 3.678541120020129E-5 0.0 1.4714164480080516E-4 1.4714164480080516E-4 20-21 0.0 7.357082240040258E-5 0.0 1.4714164480080516E-4 1.4714164480080516E-4 22-23 0.0 7.357082240040258E-5 0.0 1.4714164480080516E-4 2.391051728013084E-4 24-25 0.0 7.357082240040258E-5 0.0 2.2071246720120773E-4 3.310687008018116E-4 26-27 0.0 7.357082240040258E-5 0.0 2.2071246720120773E-4 3.678541120020129E-4 28-29 0.0 1.2874893920070452E-4 0.0 4.046395232022142E-4 3.678541120020129E-4 30-31 0.0 1.4714164480080516E-4 0.0 7.541009296041265E-4 3.8624681760211354E-4 32-33 0.0 1.4714164480080516E-4 0.0 0.001342667508807347 4.046395232022142E-4 34-35 0.0 1.655343504009058E-4 0.0 0.0018760559712102656 4.046395232022142E-4 36-37 0.0 1.8392705600100645E-4 0.0 0.002758905840015097 4.046395232022142E-4 38-39 0.0 1.8392705600100645E-4 0.0 0.0038256827648209344 4.046395232022142E-4 40-41 0.0 1.8392705600100645E-4 0.0 0.005370670035229388 4.046395232022142E-4 42-43 0.0 1.8392705600100645E-4 0.0 0.007743329057642372 4.046395232022142E-4 44-45 0.0 1.8392705600100645E-4 0.0 0.013610602144074477 4.230322288023148E-4 46-47 0.0 1.8392705600100645E-4 0.0 0.02731316781614946 4.4142493440241546E-4 48-49 0.0 1.8392705600100645E-4 0.0 0.036619876849800385 4.4142493440241546E-4 50-51 0.0 1.8392705600100645E-4 0.0 0.04813371055546339 4.4142493440241546E-4 52-53 0.0 1.8392705600100645E-4 0.0 0.06301340938594481 4.4142493440241546E-4 54-55 0.0 3.310687008018116E-4 0.0 0.08186593262604797 4.598176400025161E-4 56-57 0.0 4.046395232022142E-4 0.0 0.10310950759416421 4.7821034560261675E-4 58-59 0.0 4.598176400025161E-4 0.0 0.13027553376551287 4.7821034560261675E-4 60-61 0.0 4.7821034560261675E-4 0.0 0.165938989924108 5.149957568028181E-4 62-63 0.0 4.7821034560261675E-4 0.0 0.20997112713074895 5.149957568028181E-4 64-65 0.0 4.7821034560261675E-4 0.0 0.24068694548291703 5.149957568028181E-4 66-67 0.0 5.517811680030194E-4 0.0 0.29671112674082356 5.149957568028181E-4 68-69 0.0 5.517811680030194E-4 0.0 0.36840589317001593 5.149957568028181E-4 70-71 0.0 6.069592848033213E-4 0.0 0.41436926446466743 5.149957568028181E-4 72-73 0.0 6.621374016036232E-4 0.0 0.497982504122725 6.253519904034219E-4 74-75 0.0 6.621374016036232E-4 0.0 0.565980336726297 6.253519904034219E-4 76-77 0.0 6.621374016036232E-4 0.0 0.623531112549012 6.253519904034219E-4 78-79 0.0 6.621374016036232E-4 0.0 0.7037233089654508 6.253519904034219E-4 80-81 0.0 7.357082240040258E-4 0.0 0.7855156707690983 6.253519904034219E-4 82-83 0.0 7.357082240040258E-4 0.0 0.8895448136432675 6.621374016036232E-4 84-85 0.0 7.541009296041265E-4 0.0 1.0525593633769597 6.621374016036232E-4 86-87 0.0 7.724936352042271E-4 0.0 1.2774469747493902 6.621374016036232E-4 88-89 0.0 7.724936352042271E-4 0.0 1.548426706355673 6.621374016036232E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGGT 1040 0.0 21.92114 4 GAGTACT 7055 0.0 21.577953 12-13 TCGCGTA 160 4.99519E-5 20.779799 9 TAAGGTG 1245 0.0 20.600592 5 GTACTTT 7405 0.0 20.365633 14-15 TACATGG 9405 0.0 20.302359 2 GTACATG 9900 0.0 19.871752 1 TACACTG 2280 0.0 19.164974 5 GTACACT 1425 0.0 18.665018 4 AGTACTT 7620 0.0 18.637835 12-13 ACATGGG 9615 0.0 18.6229 3 TCTATAC 820 0.0 18.53495 3 GTGTAGC 1715 0.0 18.287352 1 AGAGTAC 12570 0.0 18.213398 10-11 CCTATAC 640 0.0 17.810928 3 ACTTTTT 8550 0.0 17.666082 16-17 GTATCAA 18600 0.0 17.142752 1 GTGTAAG 1365 0.0 17.058249 1 GAGTACA 8460 0.0 16.232988 1 AGTACAT 7850 0.0 16.216024 2 >>END_MODULE