FastQCFastQC Report
Thu 26 May 2016
SRR936456_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936456_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2819652
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT78970.2800700228255118No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT47290.16771573229604222No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39320.13944983281624826No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT35360.12540554650006455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32880.11661013486770708No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30080.10667983141181962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG104800.024.7574582
GTACATG106900.024.6276021
ACATGGG106100.023.3360773
GAGTACT61450.020.57511712-13
GTACTTT62400.020.37269814-15
GTATAGG6550.019.5890121
CATGGGG64600.019.1276554
ATACCGT3254.8567017E-1019.0106226
GTGTAGC16900.018.2774981
TATTACG1300.00754761718.2768482
GTATCAA170000.018.2538451
GTGTAAG14000.017.990281
AGAGTAC114800.017.84711810-11
GTGTAGG12550.017.4182361
AGTACTT64600.016.92302112-13
ACTTTTT78700.016.75526616-17
TCAACGC186950.016.5237584
CAACGCA187100.016.4856955
GGTATCA139900.016.3386971
GAGTACA85300.016.3233281
Produced by FastQC (version 0.11.5)