##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936456_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2819652 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.207342962890458 31.0 31.0 34.0 28.0 34.0 2 31.262199023141864 31.0 31.0 34.0 28.0 34.0 3 31.218930208408697 31.0 31.0 34.0 27.0 34.0 4 34.866859811068885 37.0 35.0 37.0 32.0 37.0 5 34.89917940228085 37.0 35.0 37.0 32.0 37.0 6 34.94894121685939 37.0 35.0 37.0 32.0 37.0 7 34.79995439153484 37.0 35.0 37.0 32.0 37.0 8 34.78427834356864 37.0 35.0 37.0 32.0 37.0 9 36.25227723137465 39.0 37.0 39.0 32.0 39.0 10-11 36.12077057736203 39.0 35.0 39.0 31.0 39.0 12-13 36.01907451699714 39.0 35.0 39.0 30.0 39.0 14-15 37.00218147487704 39.0 36.0 41.0 31.0 41.0 16-17 36.910940250782716 39.0 36.0 41.0 31.0 41.0 18-19 36.74653414676705 39.0 36.0 41.0 30.0 41.0 20-21 36.70765629942986 39.0 36.0 41.0 30.0 41.0 22-23 36.70986189075815 39.0 36.0 41.0 30.0 41.0 24-25 36.83443311444107 39.0 36.0 41.0 30.0 41.0 26-27 36.767173395865875 39.0 36.0 41.0 30.0 41.0 28-29 36.659416303855934 39.0 36.0 41.0 30.0 41.0 30-31 36.48587680323671 39.0 36.0 41.0 30.0 41.0 32-33 36.348173994521304 39.0 35.0 41.0 30.0 41.0 34-35 36.169187367802834 39.0 35.0 40.0 29.5 41.0 36-37 35.97741973122925 38.5 35.0 40.0 28.5 41.0 38-39 35.784404600284006 38.0 35.0 40.0 27.5 41.0 40-41 35.57672595767137 38.0 34.5 40.0 27.0 41.0 42-43 35.31176506888084 38.0 34.0 40.0 26.0 41.0 44-45 35.20799534836214 38.0 34.0 40.0 26.0 41.0 46-47 35.14691759834192 38.0 34.0 40.0 26.0 41.0 48-49 34.947273103205646 38.0 34.0 40.0 25.0 41.0 50-51 33.980185675395404 37.5 33.0 39.5 23.5 40.5 52-53 34.08796741583713 37.5 33.0 39.5 24.0 40.5 54-55 34.534658000348976 38.0 34.0 40.0 24.0 41.0 56-57 34.369769921962 38.0 33.5 40.0 24.0 41.0 58-59 34.1268982839017 37.5 33.0 40.0 23.0 41.0 60-61 33.81705898458391 37.0 33.0 40.0 23.0 41.0 62-63 33.47112196824289 36.5 32.5 39.5 22.0 41.0 64-65 33.09691568321197 36.0 32.0 39.0 22.0 41.0 66-67 32.716207531993305 35.5 32.0 39.0 20.5 40.5 68-69 32.26996682569339 35.0 31.0 38.5 19.5 40.0 70-71 31.866026729539673 35.0 31.0 38.0 18.5 40.0 72-73 31.44213576710885 35.0 31.0 37.0 17.5 39.5 74-75 31.03190571035007 35.0 30.0 36.5 14.5 39.0 76-77 30.57063389382803 34.0 30.0 36.0 11.5 39.0 78-79 30.105992335224347 34.0 29.5 36.0 7.5 37.5 80-81 29.663405803269338 34.0 29.0 35.0 7.0 37.0 82-83 29.2830578028778 34.0 29.0 35.0 3.5 36.5 84-85 28.914249879063092 34.0 29.0 35.0 2.0 36.0 86-87 28.57071049902612 34.0 29.0 35.0 2.0 36.0 88-89 28.21455661904377 34.0 27.5 35.0 2.0 35.5 90-91 27.862934858627945 33.5 27.0 35.0 2.0 35.0 92-93 27.436854973592485 33.0 26.0 35.0 2.0 35.0 94-95 26.933775870213772 33.0 25.0 35.0 2.0 35.0 96-97 26.320459581537012 33.0 24.5 35.0 2.0 35.0 98-99 25.570032223834716 32.5 22.0 35.0 2.0 35.0 100-101 22.977051068713443 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4142007119732405 1101 2 0.4644301334232388 1101 3 0.531859828838769 1101 4 0.6824460096836873 1101 5 0.6041013280826419 1101 6 0.5908061229837358 1101 7 0.5641145287005216 1101 8 0.46134464338619807 1101 9 0.6891264981721434 1101 10-11 0.7863339037789956 1101 12-13 0.8128173751765928 1101 14-15 0.9662084174528047 1101 16-17 0.9817638150778194 1101 18-19 1.1479644768234962 1101 20-21 1.0076622692851487 1101 22-23 0.9196173706029782 1101 24-25 0.8169674580039654 1101 26-27 0.747132926121985 1101 28-29 0.8166249704026782 1101 30-31 0.6720585689135845 1101 32-33 0.5962855223100334 1101 34-35 0.7262254094706364 1101 36-37 0.7170452086006733 1101 38-39 0.6648446020849264 1101 40-41 0.508826402237176 1101 42-43 0.6176558430188024 1101 44-45 0.5478280431909255 1101 46-47 0.6730628060592068 1101 48-49 0.7135423087921708 1101 50-51 0.6396143114153361 1101 52-53 0.4496842944044417 1101 54-55 0.5645070678138211 1101 56-57 0.6574455371176242 1101 58-59 0.6372511764106861 1101 60-61 0.44970300802195595 1101 62-63 0.5267741946086559 1101 64-65 0.30784021562089947 1101 66-67 0.4079076630579479 1101 68-69 0.4340959618995157 1101 70-71 0.2852720936692883 1101 72-73 0.586455172005266 1101 74-75 0.4815045442329229 1101 76-77 0.45790374071505013 1101 78-79 0.46863532603734726 1101 80-81 0.5518310916570606 1101 82-83 0.6723376780552321 1101 84-85 0.7189149340276266 1101 86-87 0.5708394744940541 1101 88-89 0.7699584114603546 1101 90-91 0.5675761288208783 1101 92-93 0.6586692997563333 1101 94-95 0.8634391684849234 1101 96-97 0.8835555126063284 1101 98-99 0.7625916637042067 1101 100-101 1.2299182558390704 1102 1 0.31212480979129253 1102 2 0.39924487682759136 1102 3 0.4889117906627334 1102 4 0.31244697372495267 1102 5 0.3901484375655926 1102 6 0.3641760898849853 1102 7 0.4789736180028754 1102 8 0.3255272970704439 1102 9 0.48616367803010974 1102 10-11 0.43690911506470087 1102 12-13 0.45857238015747015 1102 14-15 0.4729165378936955 1102 16-17 0.4445759234360551 1102 18-19 0.5038563756634318 1102 20-21 0.5988364715410057 1102 22-23 0.41775355749844323 1102 24-25 0.5731212547198012 1102 26-27 0.4331286200710238 1102 28-29 0.22737885066874952 1102 30-31 0.31523476352138147 1102 32-33 0.4300526742108062 1102 34-35 0.1395420327554433 1102 36-37 0.2162900429462553 1102 38-39 0.001921628981676804 1102 40-41 0.1908293586303813 1102 42-43 0.08736084639294717 1102 44-45 0.1819181489141215 1102 46-47 0.12641927638280492 1102 48-49 0.21955953299602982 1102 50-51 0.09109893495722332 1102 52-53 -0.09720024558720297 1102 54-55 0.16335343177153305 1102 56-57 0.07918145408153521 1102 58-59 0.0016427057014780644 1102 60-61 -0.003436553017927224 1102 62-63 0.0220825271386218 1102 64-65 0.14131815514337376 1102 66-67 0.042568159860550736 1102 68-69 0.09141946201198436 1102 70-71 -0.19016895520760713 1102 72-73 0.08064682340794249 1102 74-75 -0.002725347473109707 1102 76-77 0.08062619323601794 1102 78-79 -0.007111002572184333 1102 80-81 0.219036014694435 1102 82-83 0.06659038540444229 1102 84-85 0.0709667351780503 1102 86-87 -0.1531314114598672 1102 88-89 0.10419955031442996 1102 90-91 0.31503748683366695 1102 92-93 0.12804492236631404 1102 94-95 0.37568249249919106 1102 96-97 0.3148113370222809 1102 98-99 -5.514321536281841E-4 1102 100-101 0.5969608985975121 1103 1 0.4631054019226504 1103 2 0.49919030099295014 1103 3 0.5661079014125647 1103 4 0.6429367342675363 1103 5 0.5451668372617533 1103 6 0.43806767588505835 1103 7 0.5246904750635366 1103 8 0.4440522654234087 1103 9 0.5095263755750707 1103 10-11 0.46091224705232037 1103 12-13 0.4744871029484159 1103 14-15 0.8031109382595361 1103 16-17 0.863156308376638 1103 18-19 0.9033489264092722 1103 20-21 0.9299058033215033 1103 22-23 0.712878506185092 1103 24-25 0.7018964640581444 1103 26-27 0.8011371650456383 1103 28-29 0.7162010398213496 1103 30-31 0.6868932730564339 1103 32-33 0.8027515241703966 1103 34-35 0.7808047998635814 1103 36-37 0.8138170228920956 1103 38-39 0.6868883808568285 1103 40-41 0.8185355322794976 1103 42-43 0.9385897896432098 1103 44-45 0.8995247591333637 1103 46-47 1.1064453616578191 1103 48-49 1.077180392150538 1103 50-51 1.0013840123595017 1103 52-53 1.0381830993887249 1103 54-55 1.0246584339227596 1103 56-57 1.2331419672040411 1103 58-59 1.0354077108574842 1103 60-61 1.0321172454857503 1103 62-63 0.9863951220203333 1103 64-65 1.0768440991353359 1103 66-67 0.8874188951355819 1103 68-69 0.9487791394260299 1103 70-71 0.974563614064035 1103 72-73 0.9310414107133766 1103 74-75 0.8373775095151714 1103 76-77 0.7667444111467638 1103 78-79 0.7810833999869224 1103 80-81 0.9288497586661428 1103 82-83 0.735033965213649 1103 84-85 0.9574747700349349 1103 86-87 1.0887190419801485 1103 88-89 0.894897934569979 1103 90-91 0.980494565845806 1103 92-93 1.032380941357097 1103 94-95 1.062599791284157 1103 96-97 1.1878280281621691 1103 98-99 1.3873714437479414 1103 100-101 1.7159250689954781 1104 1 0.3586461498293758 1104 2 0.33671886455834965 1104 3 0.4733756956103399 1104 4 0.5278478049773057 1104 5 0.45789472030541845 1104 6 0.347999665410363 1104 7 0.3339748152799231 1104 8 0.36767287455707276 1104 9 0.5319883881180232 1104 10-11 0.5231549317216846 1104 12-13 0.6259007561936869 1104 14-15 0.7215557042467893 1104 16-17 0.6665640946238724 1104 18-19 0.7157804711491309 1104 20-21 0.7497195702650501 1104 22-23 0.5232673390124774 1104 24-25 0.630920695135309 1104 26-27 0.31591377155507416 1104 28-29 0.23713166997113433 1104 30-31 0.25945359013285696 1104 32-33 0.14731534254226375 1104 34-35 0.36491251160924776 1104 36-37 0.371285040038579 1104 38-39 0.22128541163597504 1104 40-41 0.36561381312814945 1104 42-43 0.5333151902281088 1104 44-45 0.7094517795712605 1104 46-47 0.9185231629721642 1104 48-49 0.8638590332753964 1104 50-51 0.7158625083731138 1104 52-53 0.6148448247670757 1104 54-55 0.7385192688962405 1104 56-57 0.8437750497895848 1104 58-59 0.8033992821732667 1104 60-61 0.7660028077501551 1104 62-63 0.7826541984233515 1104 64-65 0.7312749961563867 1104 66-67 0.6428802001916765 1104 68-69 0.579586195669151 1104 70-71 0.6462735569647116 1104 72-73 0.7874504215105418 1104 74-75 0.668887570005996 1104 76-77 0.6069691389936658 1104 78-79 0.4672890048395324 1104 80-81 0.6124095850886242 1104 82-83 0.5592761272601088 1104 84-85 0.49925037438715947 1104 86-87 0.554856101225571 1104 88-89 0.5335438130768324 1104 90-91 0.8123361304898111 1104 92-93 0.7161800137014502 1104 94-95 0.5791590144663736 1104 96-97 0.4916769512254042 1104 98-99 0.3181838513159647 1104 100-101 0.8651359918140855 1105 1 0.4143606259690742 1105 2 0.3225165407335915 1105 3 0.48131491181935004 1105 4 0.540009672923631 1105 5 0.37543237693031983 1105 6 0.36080813870636774 1105 7 0.44293331523549284 1105 8 0.3196541240016657 1105 9 0.3175132481553433 1105 10-11 0.5957708452558492 1105 12-13 0.6569610290098211 1105 14-15 0.8271645418900846 1105 16-17 0.8136169360883727 1105 18-19 0.6498888200338655 1105 20-21 0.6564797120050301 1105 22-23 0.5557740594425837 1105 24-25 0.4520793306102959 1105 26-27 0.5328723603758405 1105 28-29 0.6434777554594504 1105 30-31 0.35104533233493385 1105 32-33 0.49603557270310006 1105 34-35 0.35026491763431267 1105 36-37 0.3560212215012939 1105 38-39 0.2967763362782989 1105 40-41 0.2763537824696556 1105 42-43 0.3103515360914386 1105 44-45 0.5649771394752818 1105 46-47 0.6877641431552277 1105 48-49 0.7367761998087659 1105 50-51 0.7704031308737811 1105 52-53 0.9462360648808215 1105 54-55 0.729113665945917 1105 56-57 0.722952491360715 1105 58-59 0.7442443901444804 1105 60-61 0.6187569621978639 1105 62-63 0.7368864031687608 1105 64-65 0.8542488697900836 1105 66-67 0.6248948296238339 1105 68-69 0.6387072633844255 1105 70-71 0.7422841208143431 1105 72-73 0.6023867718171303 1105 74-75 0.5793551119947971 1105 76-77 0.6671921906243412 1105 78-79 0.6016777234034727 1105 80-81 0.7513466463167404 1105 82-83 0.6709354070077289 1105 84-85 0.7304475551389622 1105 86-87 0.8249273600010092 1105 88-89 0.8274958703060165 1105 90-91 0.6783259776416806 1105 92-93 0.8553352945154558 1105 94-95 0.9426449737661144 1105 96-97 1.1681896589477923 1105 98-99 1.3281234785264857 1105 100-101 1.8064767453909063 1106 1 0.10663796874337095 1106 2 0.057089362488351725 1106 3 0.16024780785653547 1106 4 0.1903735916473579 1106 5 0.32621712079713205 1106 6 0.10805425897004994 1106 7 0.24051403221293555 1106 8 0.386502442330098 1106 9 0.4382171935946886 1106 10-11 0.5288710505099132 1106 12-13 0.392035528635148 1106 14-15 0.2727285561818462 1106 16-17 0.1289475689007773 1106 18-19 0.4580307820021403 1106 20-21 0.1359833555362684 1106 22-23 0.02339561003391566 1106 24-25 -0.27868179274849325 1106 26-27 -0.17759717188381074 1106 28-29 0.0030337442084871213 1106 30-31 -0.037316761908179785 1106 32-33 -0.01578402091556086 1106 34-35 0.06323165344300463 1106 36-37 -0.09465845182978683 1106 38-39 -0.05982307076550342 1106 40-41 -0.048266877601363944 1106 42-43 0.0975893484522885 1106 44-45 -0.01036125823977585 1106 46-47 0.16253526857741463 1106 48-49 0.08947892892769005 1106 50-51 -0.025599410367085795 1106 52-53 -0.031587916179326214 1106 54-55 0.18048115761086336 1106 56-57 0.24536667018153935 1106 58-59 0.03284589316898945 1106 60-61 0.27784966318937876 1106 62-63 0.2768151072445164 1106 64-65 0.039681803837282814 1106 66-67 0.052588219139586556 1106 68-69 0.17468933351833016 1106 70-71 -0.07721143201414193 1106 72-73 0.08163173006041546 1106 74-75 0.2594080545127966 1106 76-77 0.10425977242731932 1106 78-79 0.2564410296229944 1106 80-81 0.32921851999317653 1106 82-83 0.391598084433042 1106 84-85 0.6474325466138566 1106 86-87 0.5663954153001463 1106 88-89 0.6494522456711849 1106 90-91 0.8200134050154055 1106 92-93 0.7937782414860592 1106 94-95 0.8983688473122342 1106 96-97 0.8378055949172101 1106 98-99 0.9150695368881472 1106 100-101 1.5537257435555674 1107 1 0.19260494893509517 1107 2 0.21739130680257546 1107 3 0.26880846469369857 1107 4 0.27182603344530776 1107 5 0.22185791097932395 1107 6 0.18577447111673706 1107 7 0.33171249227343225 1107 8 0.3702945076881292 1107 9 0.415561926923516 1107 10-11 0.541052235564969 1107 12-13 0.38520622303069274 1107 14-15 0.2697332034483253 1107 16-17 0.3881070654532124 1107 18-19 0.5530695427982266 1107 20-21 0.3533323004896829 1107 22-23 0.3885942446869137 1107 24-25 0.3434742589594393 1107 26-27 0.2235976851077197 1107 28-29 0.24001280485881438 1107 30-31 0.3497851535311298 1107 32-33 0.37574010034061445 1107 34-35 0.2637816828005626 1107 36-37 0.2539669574238701 1107 38-39 0.21475628090031762 1107 40-41 0.2720185160984485 1107 42-43 0.00853609941579947 1107 44-45 0.2214936591624408 1107 46-47 0.22748661980171647 1107 48-49 0.2312453182748655 1107 50-51 0.14335671948776962 1107 52-53 0.17259560992624046 1107 54-55 0.3188284086811919 1107 56-57 0.3647980190219897 1107 58-59 0.458381715900515 1107 60-61 0.40912135347569745 1107 62-63 0.33507355326206323 1107 64-65 0.29654966878494093 1107 66-67 0.31306843877360535 1107 68-69 0.3123043767614604 1107 70-71 0.3536204509283003 1107 72-73 0.32027739372389163 1107 74-75 0.33192453902547925 1107 76-77 0.3161940937472032 1107 78-79 0.4463724978662533 1107 80-81 0.4388594679801372 1107 82-83 0.4527589735286064 1107 84-85 0.35834591095694535 1107 86-87 0.43537716744509325 1107 88-89 0.4898743251602973 1107 90-91 0.47601852695526503 1107 92-93 0.5688412374851168 1107 94-95 0.47311595048255484 1107 96-97 0.7124996383328259 1107 98-99 0.6839432599181166 1107 100-101 0.9803215023006153 1108 1 0.14409251141977464 1108 2 0.16361569293211176 1108 3 0.2662614170406954 1108 4 0.29735789083210307 1108 5 0.20586556718380677 1108 6 0.23254111327232607 1108 7 0.377829528958344 1108 8 0.290372299413292 1108 9 -0.17910344734084305 1108 10-11 0.018860889461116415 1108 12-13 0.07638577328128804 1108 14-15 0.5484785807992552 1108 16-17 0.5065826930234678 1108 18-19 0.4435848180691906 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0.6556556451195021 2311 70-71 0.6530118206158129 2311 72-73 0.573667532701748 2311 74-75 0.4488591865134737 2311 76-77 0.6557373104359705 2311 78-79 0.8712488024006539 2311 80-81 0.8586908219481195 2311 82-83 0.7055346498167232 2311 84-85 0.904979447193071 2311 86-87 1.022486342410165 2311 88-89 1.050239764299274 2311 90-91 0.9267411283873273 2311 92-93 0.9904908880331611 2311 94-95 0.9454937895009152 2311 96-97 0.9460756416801424 2311 98-99 1.096337384985695 2311 100-101 1.0509953058045944 2312 1 0.28658333149321535 2312 2 0.2656570366013611 2312 3 0.299125554127599 2312 4 0.3305939849433486 2312 5 0.2994022188905916 2312 6 0.29223919568766377 2312 7 0.3335843229199895 2312 8 0.3547215446193306 2312 9 0.3734506122266765 2312 10-11 0.27368443087278393 2312 12-13 0.22486156547495284 2312 14-15 0.3534904807040604 2312 16-17 0.36325445964933323 2312 18-19 0.2765975339674611 2312 20-21 0.22046695283437856 2312 22-23 0.21787932260048137 2312 24-25 0.2131218128489465 2312 26-27 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-0.30273390346604856 2312 92-93 -0.014647306615472644 2312 94-95 -0.033488127582295135 2312 96-97 -0.20597302047804433 2312 98-99 0.17516567078398992 2312 100-101 -2.276188254164424E-4 2313 1 -0.013971468122164765 2313 2 7.414902380382671E-4 2313 3 0.08903103136775314 2313 4 0.09312632406415844 2313 5 0.03810030847857604 2313 6 0.13713696233800476 2313 7 0.2599541772352012 2313 8 0.1744900454992404 2313 9 0.08587935730550811 2313 10-11 0.15291500472208952 2313 12-13 0.1196094745444185 2313 14-15 0.1483367224718677 2313 16-17 0.1794265600140008 2313 18-19 0.023996978805001845 2313 20-21 0.10294958132908505 2313 22-23 0.10963824420646517 2313 24-25 0.04074513692957282 2313 26-27 -0.12009689217600794 2313 28-29 0.12347439383720626 2313 30-31 0.17999909863382157 2313 32-33 0.0011313931810761346 2313 34-35 -0.09992598262521568 2313 36-37 -0.036407037776641005 2313 38-39 0.11398248633769015 2313 40-41 0.2077116784359987 2313 42-43 0.08905514641674728 2313 44-45 0.32093117071557486 2313 46-47 0.14832747127519497 2313 48-49 0.15267160207814356 2313 50-51 0.07859876589276382 2313 52-53 0.2179486974583611 2313 54-55 -6.776570803381787E-4 2313 56-57 0.18539980932744982 2313 58-59 0.22344631823425232 2313 60-61 0.19447505313829083 2313 62-63 0.16460953084487784 2313 64-65 0.06960521599158653 2313 66-67 0.1978290985137292 2313 68-69 -0.011924066365068597 2313 70-71 -0.10454703743544158 2313 72-73 0.16234096259204023 2313 74-75 0.18449919169373175 2313 76-77 0.16781300818226796 2313 78-79 0.10717785017651593 2313 80-81 0.21616447772853675 2313 82-83 0.10783644711644413 2313 84-85 0.03210420815971915 2313 86-87 0.08268663936036447 2313 88-89 0.22926539406670088 2313 90-91 0.2347172476796544 2313 92-93 0.5334150675204867 2313 94-95 0.33526438969849437 2313 96-97 0.25297797645585973 2313 98-99 0.38271787601267704 2313 100-101 0.1530157273891355 2314 1 -0.4882665307415657 2314 2 -0.38605353358870076 2314 3 -0.5382787268315496 2314 4 -0.33829014602428487 2314 5 -0.2355766922084328 2314 6 -0.10089044401679814 2314 7 -0.35043163561589097 2314 8 -0.3108655809137417 2314 9 -0.43224532150561856 2314 10-11 -0.3992324478528744 2314 12-13 -0.7081560485835325 2314 14-15 -0.8948080164558121 2314 16-17 -1.0148351374678342 2314 18-19 -1.429103957172856 2314 20-21 -1.3214311067527404 2314 22-23 -1.1288741566294362 2314 24-25 -0.9964849566590104 2314 26-27 -1.0234551758001302 2314 28-29 -0.8950210278815547 2314 30-31 -1.0476045757611487 2314 32-33 -0.9121452258550917 2314 34-35 -1.252664819275239 2314 36-37 -0.9416728859008927 2314 38-39 -1.0138148525201771 2314 40-41 -0.849323592082186 2314 42-43 -1.0551237876390687 2314 44-45 -0.9224821513627077 2314 46-47 -1.0045944667395332 2314 48-49 -1.4045657351061749 2314 50-51 -0.9125907596517138 2314 52-53 -0.8816468933504353 2314 54-55 -1.0023324083653549 2314 56-57 -0.9811409392318495 2314 58-59 -1.0069810596611077 2314 60-61 -1.1214061224667233 2314 62-63 -1.0777468492376912 2314 64-65 -1.167862081794329 2314 66-67 -0.9961343839707801 2314 68-69 -0.6912670255658853 2314 70-71 -0.8884935764345983 2314 72-73 -0.8729622563713306 2314 74-75 -0.773220484829487 2314 76-77 -0.715185737004429 2314 78-79 -0.46873324003061967 2314 80-81 -0.48977042025365947 2314 82-83 -0.5646277972559481 2314 84-85 -0.6327763294065782 2314 86-87 -0.4607599352131011 2314 88-89 -0.38686357182207587 2314 90-91 -0.557598448957819 2314 92-93 -0.4934937642065975 2314 94-95 -0.5647050244426524 2314 96-97 -0.7459235302971088 2314 98-99 -0.7837341379241778 2314 100-101 -1.372693060522355 2315 1 -1.0854886739969416 2315 2 -1.404125644776105 2315 3 -1.2308714544419708 2315 4 -0.9945776703734808 2315 5 -1.052314334277689 2315 6 -0.6847845069993426 2315 7 -0.5325090449027741 2315 8 -0.553729774249561 2315 9 -0.8095587667875535 2315 10-11 -1.1300669988549927 2315 12-13 -0.9822068327020332 2315 14-15 -0.9873772100876153 2315 16-17 -1.0959443648944713 2315 18-19 -1.0622292974038459 2315 20-21 -1.2332105709650918 2315 22-23 -1.050324235442062 2315 24-25 -1.5083571855497553 2315 26-27 -1.5136838924045506 2315 28-29 -1.5692060259122158 2315 30-31 -1.3035689789183849 2315 32-33 -1.5656181487919625 2315 34-35 -1.279821926921393 2315 36-37 -1.449095119256512 2315 38-39 -1.3898957460844912 2315 40-41 -1.6671796276781379 2315 42-43 -1.869072079106779 2315 44-45 -1.9055533500649702 2315 46-47 -2.2101737476521777 2315 48-49 -2.863011261749463 2315 50-51 -2.743100907411879 2315 52-53 -2.4438047462578254 2315 54-55 -2.492379408318193 2315 56-57 -2.6984217691618966 2315 58-59 -2.724320975373562 2315 60-61 -2.9828101634214192 2315 62-63 -3.2102613762576766 2315 64-65 -2.638801631576275 2315 66-67 -2.5627304152568158 2315 68-69 -2.659599944741327 2315 70-71 -2.505071776077429 2315 72-73 -2.536625637311083 2315 74-75 -2.4957255570094574 2315 76-77 -2.4292065925018953 2315 78-79 -2.462187259976421 2315 80-81 -2.75358793704768 2315 82-83 -2.6554596459196453 2315 84-85 -2.56265594268233 2315 86-87 -2.8086836106520003 2315 88-89 -2.811309968698602 2315 90-91 -2.5507750444328074 2315 92-93 -2.300757391255356 2315 94-95 -2.6275585845672857 2315 96-97 -2.457372482020567 2315 98-99 -2.594936389783676 2315 100-101 -2.949717843886564 2316 1 -5.51181176637381 2316 2 -5.525384861804273 2316 3 -6.1856899328657455 2316 4 -8.160440107518063 2316 5 -8.857768516624503 2316 6 -9.68705085368493 2316 7 -11.56748977500558 2316 8 -11.680297092072514 2316 9 -11.332988901462993 2316 10-11 -11.517173442397713 2316 12-13 -9.91992562497057 2316 14-15 -10.178599560862594 2316 16-17 -9.138838899443613 2316 18-19 -9.48392058558057 2316 20-21 -10.532683331106846 2316 22-23 -10.060013853052777 2316 24-25 -10.104195869566212 2316 26-27 -7.930146665968099 2316 28-29 -6.265811414938021 2316 30-31 -8.370650460664326 2316 32-33 -5.975286159126263 2316 34-35 -4.615638795398535 2316 36-37 -5.092459398226161 2316 38-39 -3.9041143380859324 2316 40-41 -3.381518417127687 2316 42-43 -3.646421554302851 2316 44-45 -4.15623810734013 2316 46-47 -4.016277078036364 2316 48-49 -4.716606530313776 2316 50-51 -4.619573856105941 2316 52-53 -4.528897064369005 2316 54-55 -3.972235736665496 2316 56-57 -4.6960029442779 2316 58-59 -4.814046027429992 2316 60-61 -5.015861300518289 2316 62-63 -4.5472030503716 2316 64-65 -5.235864418007502 2316 66-67 -4.615387087535424 2316 68-69 -4.864779071294137 2316 70-71 -4.48749196954623 2316 72-73 -3.5045059188676575 2316 74-75 -3.7887853072650444 2316 76-77 -4.249816178025245 2316 78-79 -4.512213683332508 2316 80-81 -5.060154838344818 2316 82-83 -4.388588398138481 2316 84-85 -4.486619341964339 2316 86-87 -5.213827853168201 2316 88-89 -6.683804166159359 2316 90-91 -5.655695478705589 2316 92-93 -5.024950177251462 2316 94-95 -4.481116291429068 2316 96-97 -5.87977744254545 2316 98-99 -7.584503162032199 2316 100-101 -8.672521590684989 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7577.0 3 3057.0 4 3281.0 5 3549.0 6 3800.0 7 4379.0 8 5515.0 9 6961.0 10 8736.0 11 10086.0 12 11840.0 13 13537.0 14 14906.0 15 17002.0 16 19163.0 17 22301.0 18 19885.0 19 18223.0 20 19491.0 21 21453.0 22 24077.0 23 27275.0 24 31472.0 25 36549.0 26 42495.0 27 49837.0 28 59734.0 29 70815.0 30 84381.0 31 103392.0 32 127945.0 33 160736.0 34 209372.0 35 280813.0 36 386417.0 37 488843.0 38 354655.0 39 46093.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.557498016820205 17.85156409441888 14.029306694540622 23.561631194220293 2 15.859444678195606 19.329868928274184 39.685471848989025 25.125214544541187 3 20.346066983704375 22.80940528649206 28.687904433242778 28.156623296560788 4 13.206371864244925 15.648818134541887 36.29873949013451 34.84607051107868 5 14.630713917027476 36.77844254022424 33.631205246045866 14.959638296702419 6 33.71909821767186 34.56933958532374 17.012348473372217 14.699213723632177 7 30.09355765780379 31.41874780605003 19.86514550435915 18.62254903178703 8 26.9595722453429 33.91803734762589 19.294806647400485 19.827583759630727 9 26.298424974961016 16.963159504913037 19.85320161867912 36.88521390144683 10-11 25.83841839387202 25.671449787873573 26.734832206310028 21.755299611944377 12-13 26.750559700830596 22.873580346783893 26.953755051499574 23.42210490088594 14-15 23.41147240654838 24.67102496857062 25.17684015778737 26.740662467093625 16-17 23.24791092875173 27.611795964269582 25.808879657359313 23.331413449619376 18-19 23.166011382934045 26.713468338519945 27.38144433063206 22.739075947913946 20-21 24.066033992842026 25.75957484205472 27.224584782306792 22.949806382796456 22-23 23.519587522454945 25.625586036602204 27.354960430170156 23.4998660107727 24-25 23.650473148519964 25.774817062009486 27.377732718266273 23.196977071204277 26-27 23.29937125034765 25.88531881623085 27.565922222915944 23.24938771050555 28-29 23.107343490586683 26.547892275940487 27.229564636953917 23.115199596518913 30-31 23.410729710161245 25.828289951888898 27.112292988468834 23.64868734948102 32-33 23.25265515830573 25.893262343491212 27.242020869552263 23.612061628650792 34-35 23.948981169874067 25.935813418309152 26.968512176020248 23.146693235796533 36-37 23.76644668299919 25.72335258301258 27.204983813777307 23.30521692021092 38-39 23.409603959184476 25.98431173505698 27.263091007791285 23.342993297967258 40-41 23.685129400273414 25.929282806882647 27.063409405799167 23.322178387044776 42-43 23.479010131450124 25.959054634429553 26.7517240559919 23.810211178128423 44-45 23.606743426010812 25.878934684808495 26.873779762274197 23.640542126906496 46-47 24.05088386393069 26.2110204630987 26.54212697274986 23.195968700220757 48-49 23.887260614735755 26.127280362091604 26.534693706736164 23.45076531643648 50-51 23.440234456252334 26.377215028706775 26.85974279253966 23.322807722501228 52-53 23.87902674376376 26.3874958860113 26.30023322060058 23.43324414962435 54-55 23.490943176474175 26.208398087416775 26.4875470006476 23.813111735461455 56-57 23.770057624801275 26.262297029738086 26.285616498479804 23.682028846980838 58-59 24.11578995341965 26.00402583899871 26.27071860924659 23.609465598335046 60-61 23.655252875644376 26.18118080218876 26.861620958478966 23.301945363687903 62-63 23.332287666911323 26.30122960085985 26.852642784449493 23.513839947779335 64-65 23.350003927812654 26.561624720642087 26.432920141628237 23.65545120991703 66-67 23.43021571201136 26.325231705193463 26.247597653458477 23.9969549293367 68-69 23.518693520394148 26.17552148900541 26.570833288261074 23.734951702339366 70-71 23.74384348268337 26.066092277093023 26.340282730753913 23.849781509469697 72-73 23.57927849983133 26.05109251614821 26.66670923430398 23.702919749716482 74-75 23.441577271344695 26.7060280986708 26.176067622680062 23.676327007304447 76-77 23.698337835656627 26.24389171010832 26.22517883653817 23.83259161769688 78-79 23.60150346868359 26.390905404988363 26.31486998777261 23.692721138555438 80-81 23.782579340440442 26.295995393167182 26.26776133924475 23.653663927147626 82-83 23.73652252865736 26.343242821473154 26.38406537282942 23.536169277040063 84-85 23.674699863453387 26.507651930272562 26.181784327286266 23.63586387898778 86-87 23.617098471873817 26.509018842054378 26.200765570398893 23.673117115672916 88-89 23.58877045593024 26.476398408567466 25.882856350444282 24.05197478505801 90-91 23.67305962568666 26.284272568892725 26.136017018885916 23.90665078653471 92-93 23.767434469661904 26.289759706428057 26.3547571189458 23.588048704964244 94-95 23.513096449365285 26.199744507106747 26.511777129892227 23.77538191363574 96-97 23.275936830564536 26.208785742167446 26.715328751946622 23.799948675321396 98-99 23.21630548972748 26.49035482953805 26.159085051923526 24.134254628810947 100-101 23.35011939070744 26.078875019044162 26.31249522233205 24.258510367916347 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 55.0 1 53.5 2 67.5 3 88.5 4 81.5 5 84.5 6 144.5 7 284.0 8 501.0 9 637.5 10 657.0 11 655.0 12 717.5 13 799.5 14 892.5 15 1069.0 16 1237.0 17 1394.5 18 1583.0 19 1782.0 20 2028.0 21 2356.0 22 2837.5 23 3600.0 24 4665.0 25 6010.5 26 7970.0 27 10821.0 28 13791.0 29 17545.5 30 22499.5 31 27865.5 32 34389.5 33 39283.5 34 43405.5 35 48692.0 36 55334.0 37 61674.5 38 65765.5 39 69595.5 40 73747.5 41 78711.0 42 82857.5 43 87484.0 44 93002.5 45 99496.5 46 104940.0 47 112680.0 48 126155.5 49 126552.0 50 114537.0 51 108174.0 52 107589.0 53 108254.5 54 108161.0 55 102463.0 56 93408.0 57 85900.5 58 80475.0 59 72648.5 60 61478.0 61 51787.5 62 43089.5 63 34049.5 64 24985.0 65 18679.5 66 14694.0 67 11789.5 68 9131.0 69 7171.0 70 5709.5 71 4136.5 72 3130.5 73 2691.5 74 2086.5 75 1482.0 76 1131.5 77 749.5 78 493.0 79 331.0 80 217.5 81 161.0 82 120.5 83 74.0 84 40.5 85 29.0 86 19.0 87 12.5 88 13.0 89 12.0 90 8.0 91 7.0 92 6.0 93 5.0 94 4.5 95 1.0 96 0.0 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07827207045408441 2 0.03156417884192801 3 0.015214643509199008 4 0.022485044253688045 5 0.02720193839523459 6 0.03273453603494332 7 0.07961975449452627 8 0.056319006742676044 9 0.07256214596694911 10-11 0.06231265418569383 12-13 0.04192006673163923 14-15 0.05068001299451138 16-17 0.06288010009745884 18-19 0.0589257113998465 20-21 0.01980740885754696 22-23 0.014026553631441042 24-25 0.023549005338247414 26-27 0.025676927507366155 28-29 0.006702954832724038 30-31 0.0011526245082726521 32-33 0.009983501510115433 34-35 0.012058225625006207 36-37 0.00441543850092139 38-39 0.010054432249086058 40-41 0.004450903870406702 42-43 0.0023229817012879604 44-45 6.029112812503104E-4 46-47 5.497132270223417E-4 48-49 0.0012058225625006208 50-51 7.62505443934216E-4 52-53 1.418614779412495E-4 54-55 3.546536948531237E-4 56-57 0.004238111653494829 58-59 0.004096250175553579 60-61 0.005443934215995449 62-63 0.002784031504597021 64-65 5.851785965076541E-4 66-67 4.610498033090608E-4 68-69 0.0037415964807004554 70-71 0.002801764189339677 72-73 0.021598410016555235 74-75 0.01700564466820728 76-77 0.026137977310675218 78-79 0.020250725976113364 80-81 0.012820731068940421 82-83 0.004681428772061233 84-85 0.0036174676875018615 86-87 0.0019505953216921806 88-89 0.001915129952206868 90-91 0.006951212419121226 92-93 0.010958799170961523 94-95 0.014026553631441042 96-97 0.012253285157175423 98-99 0.023070222850195696 100-101 0.01938182442372321 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2819652.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.92626761958164 #Duplication Level Percentage of deduplicated Percentage of total 1 77.41127675858095 44.0673505753248 2 12.968158778303076 14.764577542938156 3 4.1678717092874225 7.11784140980937 4 1.8244382882484662 4.154338490089745 5 0.9620409178682753 2.73826993757787 6 0.5855232755627785 1.9998992809308467 7 0.38787237016238324 1.5456088442273686 8 0.2777034777433628 1.2646897994325765 9 0.20350304300029345 1.042620181851054 >10 1.0880095313238813 11.290789396586847 >50 0.0811465835744152 3.200033025965327 >100 0.03825583014243797 3.8704254281902233 >500 0.002757838699999899 1.070267259703512 >1k 0.0013789193498994342 1.5915212400468983 >5k 6.26781522681561E-5 0.2817675873254199 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7897 0.2800700228255118 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4729 0.16771573229604222 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3932 0.13944983281624826 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3536 0.12540554650006455 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3288 0.11661013486770708 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3008 0.10667983141181962 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0639610845593712E-4 2 0.0 0.0 0.0 0.0 1.0639610845593712E-4 3 0.0 0.0 0.0 0.0 1.0639610845593712E-4 4 0.0 0.0 0.0 0.0 1.418614779412495E-4 5 0.0 7.093073897062475E-5 0.0 0.0 1.418614779412495E-4 6 0.0 7.093073897062475E-5 0.0 0.0 1.418614779412495E-4 7 0.0 7.093073897062475E-5 0.0 0.0 1.418614779412495E-4 8 0.0 7.093073897062475E-5 0.0 0.0 1.418614779412495E-4 9 0.0 7.093073897062475E-5 0.0 0.0 1.418614779412495E-4 10-11 0.0 7.093073897062475E-5 0.0 0.0 1.418614779412495E-4 12-13 0.0 8.866342371328093E-5 0.0 0.0 1.418614779412495E-4 14-15 0.0 1.0639610845593712E-4 0.0 0.0 1.418614779412495E-4 16-17 0.0 1.0639610845593712E-4 0.0 0.0 1.418614779412495E-4 18-19 0.0 1.418614779412495E-4 0.0 0.0 1.418614779412495E-4 20-21 0.0 1.418614779412495E-4 0.0 0.0 1.418614779412495E-4 22-23 0.0 1.418614779412495E-4 0.0 1.7732684742656188E-5 2.305249016545304E-4 24-25 0.0 1.418614779412495E-4 0.0 7.093073897062475E-5 3.5465369485312374E-4 26-27 0.0 1.418614779412495E-4 0.0 1.241287931985933E-4 3.5465369485312374E-4 28-29 0.0 1.418614779412495E-4 0.0 3.723863795957799E-4 3.5465369485312374E-4 30-31 0.0 1.418614779412495E-4 0.0 7.447727591915598E-4 3.5465369485312374E-4 32-33 0.0 1.418614779412495E-4 0.0 0.0011880898777579644 3.723863795957799E-4 34-35 0.0 1.418614779412495E-4 0.0 0.0020392587454054613 3.901190643384361E-4 36-37 0.0 1.418614779412495E-4 0.0 0.002872694928310302 3.901190643384361E-4 38-39 0.0 1.418614779412495E-4 0.0 0.004149448229781547 3.901190643384361E-4 40-41 0.0 1.418614779412495E-4 0.0 0.005940449388789822 3.901190643384361E-4 42-43 0.0 1.418614779412495E-4 0.0 0.008352094513791064 4.078517490810923E-4 44-45 0.0 1.5959416268390567E-4 0.0 0.014150682424639637 4.255844338237485E-4 46-47 0.0 1.9505953216921807E-4 0.0 0.02636850221232975 4.610498033090608E-4 48-49 0.0 2.1279221691187424E-4 0.0 0.03530577532262846 5.319805422796856E-4 50-51 0.0 2.1279221691187424E-4 0.0 0.0464241686562739 5.319805422796856E-4 52-53 0.0 2.305249016545304E-4 0.0 0.061426019948561034 5.319805422796856E-4 54-55 0.0 3.5465369485312374E-4 0.0 0.07836073387779768 5.319805422796856E-4 56-57 0.0 3.901190643384361E-4 0.0 0.09857599448442574 5.319805422796856E-4 58-59 0.0 4.965151727943732E-4 0.0 0.12593752704234423 5.319805422796856E-4 60-61 0.0 4.965151727943732E-4 0.0 0.1582110132739785 5.497132270223417E-4 62-63 0.0 4.965151727943732E-4 0.0 0.19610576056903475 5.67445911764998E-4 64-65 1.7732684742656188E-5 4.965151727943732E-4 0.0 0.22731528571610965 5.67445911764998E-4 66-67 3.5465369485312375E-5 5.319805422796856E-4 0.0 0.28228660841834385 5.67445911764998E-4 68-69 3.5465369485312375E-5 5.319805422796856E-4 0.0 0.35335920886690986 5.67445911764998E-4 70-71 3.5465369485312375E-5 5.319805422796856E-4 0.0 0.3998011102079264 5.851785965076541E-4 72-73 3.5465369485312375E-5 5.319805422796856E-4 0.0 0.48225809426127764 6.738420202209351E-4 74-75 3.5465369485312375E-5 5.319805422796856E-4 0.0 0.5455637787925602 6.738420202209351E-4 76-77 3.5465369485312375E-5 5.319805422796856E-4 0.0 0.6024324987622587 6.915747049635913E-4 78-79 3.5465369485312375E-5 5.319805422796856E-4 0.0 0.6770693688440985 7.093073897062475E-4 80-81 3.5465369485312375E-5 5.319805422796856E-4 0.0 0.7591185011483687 7.093073897062475E-4 82-83 3.5465369485312375E-5 5.319805422796856E-4 0.0 0.8615424882219508 7.447727591915598E-4 84-85 3.5465369485312375E-5 5.319805422796856E-4 0.0 1.0216154333939083 7.447727591915598E-4 86-87 3.5465369485312375E-5 5.319805422796856E-4 0.0 1.241961774006154 7.447727591915598E-4 88-89 3.5465369485312375E-5 5.319805422796856E-4 0.0 1.5141052867516984 7.447727591915598E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 10480 0.0 24.757458 2 GTACATG 10690 0.0 24.627602 1 ACATGGG 10610 0.0 23.336077 3 GAGTACT 6145 0.0 20.575117 12-13 GTACTTT 6240 0.0 20.372698 14-15 GTATAGG 655 0.0 19.589012 1 CATGGGG 6460 0.0 19.127655 4 ATACCGT 325 4.8567017E-10 19.010622 6 GTGTAGC 1690 0.0 18.277498 1 TATTACG 130 0.007547617 18.276848 2 GTATCAA 17000 0.0 18.253845 1 GTGTAAG 1400 0.0 17.99028 1 AGAGTAC 11480 0.0 17.847118 10-11 GTGTAGG 1255 0.0 17.418236 1 AGTACTT 6460 0.0 16.923021 12-13 ACTTTTT 7870 0.0 16.755266 16-17 TCAACGC 18695 0.0 16.523758 4 CAACGCA 18710 0.0 16.485695 5 GGTATCA 13990 0.0 16.338697 1 GAGTACA 8530 0.0 16.323328 1 >>END_MODULE