FastQCFastQC Report
Thu 26 May 2016
SRR936456_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936456_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2819652
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT87750.31120861723361604No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47300.1677511976655275No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT45530.16147382726662723No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41450.14700395651661977No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT35760.12682416127947704No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC32690.11593629284748613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT9850.024.1054174
TACATGG102400.021.5684342
GTACATG105600.021.4149231
GTGTAGC17650.021.2645341
GAGTACT76050.020.6384112-13
GTACTTT77650.020.1825714-15
ACATGGG105300.020.071243
AGTACTT79250.018.90619512-13
AGAGTAC133850.018.76834310-11
GTATCAA189050.018.7220361
TAAGGTG13300.017.852515
GTATTAG9350.017.784021
CATGGGG64150.017.2480564
GGTAAGG11600.017.1962533
TCAACGC211300.016.5633774
ACTTTTT95650.016.5331516-17
CAACGCA212650.016.5028885
ATCAACG212800.016.4489573
AACGCAG215300.016.2777086
TATCAAC216800.016.255872
Produced by FastQC (version 0.11.5)