##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936456_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2819652 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.91184621364622 31.0 31.0 34.0 30.0 34.0 2 32.076973683277224 33.0 31.0 34.0 30.0 34.0 3 32.17319867841847 33.0 31.0 34.0 30.0 34.0 4 35.84987580027606 37.0 35.0 37.0 35.0 37.0 5 35.67465595045062 37.0 35.0 37.0 33.0 37.0 6 35.749849981487074 37.0 35.0 37.0 33.0 37.0 7 35.69307489009282 37.0 35.0 37.0 33.0 37.0 8 35.66127025604578 37.0 35.0 37.0 33.0 37.0 9 37.30456418026054 39.0 37.0 39.0 34.0 39.0 10-11 37.19841030027819 39.0 37.0 39.0 33.0 39.0 12-13 37.09653496247054 39.0 37.0 39.0 33.0 39.0 14-15 38.26215185420045 40.0 38.0 41.0 33.0 41.0 16-17 38.152469879261695 40.0 37.0 41.0 33.0 41.0 18-19 38.268364145646345 40.0 38.0 41.0 33.5 41.0 20-21 38.27001825047914 40.0 38.0 41.0 33.5 41.0 22-23 38.1862855770854 40.0 37.5 41.0 33.0 41.0 24-25 38.049381448490806 40.0 37.0 41.0 33.0 41.0 26-27 37.85890634730811 40.0 37.0 41.0 33.0 41.0 28-29 37.79769985799666 40.0 37.0 41.0 32.5 41.0 30-31 37.8215247839095 40.0 37.0 41.0 33.0 41.0 32-33 37.69952994199284 40.0 37.0 41.0 33.0 41.0 34-35 37.55049718901481 40.0 37.0 41.0 32.0 41.0 36-37 37.37480547244837 39.5 36.0 41.0 31.5 41.0 38-39 37.222879631954584 39.0 36.0 41.0 31.0 41.0 40-41 37.05900533115434 39.0 36.0 41.0 31.0 41.0 42-43 36.8092074837604 39.0 36.0 41.0 31.0 41.0 44-45 36.59198511021927 39.0 35.0 40.5 30.0 41.0 46-47 36.306367771625716 39.0 35.0 40.0 30.0 41.0 48-49 35.98284699672158 39.0 35.0 40.0 29.0 41.0 50-51 35.720194194177154 38.0 35.0 40.0 28.0 41.0 52-53 35.367420873214144 38.0 34.0 40.0 27.5 41.0 54-55 35.18036197374711 38.0 34.0 40.0 27.0 41.0 56-57 34.86172726279697 38.0 34.0 40.0 26.5 41.0 58-59 34.483099510152314 37.0 33.5 40.0 26.0 41.0 60-61 34.052597802849434 37.0 33.0 39.5 26.0 41.0 62-63 33.72311724993013 36.0 32.5 39.0 25.0 40.5 64-65 33.299646374800865 36.0 32.0 39.0 24.5 40.0 66-67 32.87130840969027 35.0 31.5 38.5 23.0 40.0 68-69 32.49815172226927 35.0 31.0 38.0 22.0 40.0 70-71 32.12100110226368 35.0 31.0 37.5 22.0 39.5 72-73 31.66193948756797 34.5 31.0 37.0 21.0 39.0 74-75 31.17264009884908 34.0 30.0 36.0 20.0 39.0 76-77 29.65971988741873 32.5 28.5 35.0 18.5 37.0 78-79 30.445009525998245 34.0 30.0 35.0 18.5 37.0 80-81 30.235876625909864 34.0 30.0 35.0 18.0 37.0 82-83 29.9482753545473 34.0 29.5 35.0 16.5 36.5 84-85 29.58236956191757 34.0 29.0 35.0 12.0 36.0 86-87 29.22601352932915 34.0 29.0 35.0 7.0 36.0 88-89 28.77175197506643 33.0 29.0 35.0 4.5 35.0 90-91 28.318856901489973 33.0 28.0 35.0 2.0 35.0 92-93 27.717977431257474 33.0 27.0 35.0 2.0 35.0 94-95 27.17577293226256 32.5 26.0 34.0 2.0 35.0 96-97 26.46206482218373 32.0 25.0 34.0 2.0 35.0 98-99 25.588286781489344 32.0 23.5 34.0 2.0 35.0 100-101 22.34231635677027 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32115333777176147 1101 2 0.30941582245306165 1101 3 0.2950720368747852 1101 4 0.27809053171503706 1101 5 0.3700601872467786 1101 6 0.3135185548967385 1101 7 0.3445673459529601 1101 8 0.31298800674834126 1101 9 0.26642492740819534 1101 10-11 0.3728167091077381 1101 12-13 0.4050393116860391 1101 14-15 0.6177446480571618 1101 16-17 0.5267232666819339 1101 18-19 0.46762008406807354 1101 20-21 0.39282099978617424 1101 22-23 0.22604731884896978 1101 24-25 0.3404856629470956 1101 26-27 0.42309368286743165 1101 28-29 0.27641050092808683 1101 30-31 0.40943840003321696 1101 32-33 0.39396427234471076 1101 34-35 0.4097155606184302 1101 36-37 0.5235570761042467 1101 38-39 0.4224853383310503 1101 40-41 0.3463999622486895 1101 42-43 0.5696652409594876 1101 44-45 0.4656605268731866 1101 46-47 0.4336051482700185 1101 48-49 0.2858822450881746 1101 50-51 0.37809334102163916 1101 52-53 0.479046739881845 1101 54-55 0.5312131067875256 1101 56-57 0.3329693528077726 1101 58-59 0.3845847724398297 1101 60-61 0.45985589476306643 1101 62-63 0.3735294391524633 1101 64-65 0.3815828025179613 1101 66-67 0.2478050646781611 1101 68-69 0.15208017169839394 1101 70-71 0.2002775821026077 1101 72-73 0.3623861627485745 1101 74-75 0.1704193617143943 1101 76-77 0.16851304272806544 1101 78-79 0.24376581470881575 1101 80-81 0.47919333639096706 1101 82-83 0.5026936617109428 1101 84-85 0.4392942834313587 1101 86-87 0.29361750829097844 1101 88-89 0.25833449954186705 1101 90-91 0.33514787195135654 1101 92-93 0.33602282924060844 1101 94-95 0.31879945992635683 1101 96-97 0.35544949674906334 1101 98-99 0.3786411295428742 1101 100-101 0.858241948965123 1102 1 0.11881714444935199 1102 2 0.13958388697955826 1102 3 0.1701322894535977 1102 4 0.13473759620939774 1102 5 0.1165494703214307 1102 6 0.10404845624318426 1102 7 0.1058385950290841 1102 8 0.21062289218810548 1102 9 0.17739571695798872 1102 10-11 0.18456355187261408 1102 12-13 0.2619032854641148 1102 14-15 0.30419022792798245 1102 16-17 0.2680629626875941 1102 18-19 0.15855648947955814 1102 20-21 0.15593196093531247 1102 22-23 0.15199288265243638 1102 24-25 0.14998307610304806 1102 26-27 0.21142235666799536 1102 28-29 0.08594004879273598 1102 30-31 0.2224063617286518 1102 32-33 0.13658935669831607 1102 34-35 0.18656410973633086 1102 36-37 0.17026005499174346 1102 38-39 0.10489801226016482 1102 40-41 0.1451161806361725 1102 42-43 0.18035324507273032 1102 44-45 0.07153635835877736 1102 46-47 -0.02767252774784623 1102 48-49 0.03616342378929005 1102 50-51 -0.07149533470701641 1102 52-53 0.12555691051714035 1102 54-55 0.01981331887660076 1102 56-57 -0.1393947977112049 1102 58-59 0.02545530169847865 1102 60-61 0.08134116099912347 1102 62-63 0.1315309816184822 1102 64-65 -0.0648406044038552 1102 66-67 -0.19644121572930118 1102 68-69 -0.16043790407524483 1102 70-71 -0.11099527370895146 1102 72-73 -0.021029114091938084 1102 74-75 -0.12444872530639728 1102 76-77 -0.2152717832624873 1102 78-79 -0.058204042934420386 1102 80-81 -0.05862118207042144 1102 82-83 0.014462998771900715 1102 84-85 0.04108900691218764 1102 86-87 0.0895058723129587 1102 88-89 -0.01097846038614847 1102 90-91 -0.11143620851395397 1102 92-93 -0.03959174706325541 1102 94-95 -0.22614896386618355 1102 96-97 0.037042735606352295 1102 98-99 0.026846406062045247 1102 100-101 0.9082194546690303 1103 1 0.25426647025685156 1103 2 0.3356763595719272 1103 3 0.2527859836673514 1103 4 0.1682507410166707 1103 5 0.2514062480515662 1103 6 0.1990634125075772 1103 7 0.19361320597064235 1103 8 0.19434591452691308 1103 9 0.26423782318310174 1103 10-11 0.35128559857241726 1103 12-13 0.27043839037363426 1103 14-15 0.3502931002691412 1103 16-17 0.25199672158814934 1103 18-19 0.23621410050555625 1103 20-21 0.22924123411167585 1103 22-23 0.0573334186799741 1103 24-25 0.2508061732688631 1103 26-27 0.1779075669092478 1103 28-29 0.2163667894164263 1103 30-31 0.21900460206921935 1103 32-33 0.10189605059076001 1103 34-35 0.2139192872942246 1103 36-37 0.16130164923485069 1103 38-39 0.1745931442638735 1103 40-41 0.2036479408110239 1103 42-43 0.1899085854442646 1103 44-45 0.20208007330988664 1103 46-47 0.287666864124553 1103 48-49 0.2766041538114479 1103 50-51 0.36385423393187466 1103 52-53 0.3462752206182813 1103 54-55 0.424670787486356 1103 56-57 0.20507668486163055 1103 58-59 0.2423712150687578 1103 60-61 0.25091463970230876 1103 62-63 0.3221814063997783 1103 64-65 0.2872959865182807 1103 66-67 0.25631998154680957 1103 68-69 0.31020994727085593 1103 70-71 0.31732797607180885 1103 72-73 0.5184650742944861 1103 74-75 0.40456218730171756 1103 76-77 0.3314066393240864 1103 78-79 0.09158341105719714 1103 80-81 0.23877983114658008 1103 82-83 0.3171454028721463 1103 84-85 0.5990081140471482 1103 86-87 0.667962198668409 1103 88-89 0.49517786191174906 1103 90-91 0.5736180913343816 1103 92-93 0.5069866990278449 1103 94-95 0.4209606918893378 1103 96-97 0.644511071204235 1103 98-99 0.4370039083384718 1103 100-101 0.8507349802694115 1104 1 0.2477792050201657 1104 2 0.27065115969646314 1104 3 0.2069286041208116 1104 4 0.19773966863895964 1104 5 0.11993427231460885 1104 6 0.07740868555024605 1104 7 0.15149654152790504 1104 8 0.11819239127019898 1104 9 0.18458543690918106 1104 10-11 0.20578012076165209 1104 12-13 0.23029285518554587 1104 14-15 0.3733333338926954 1104 16-17 0.3782878155935592 1104 18-19 0.22769923911182133 1104 20-21 0.18584963388483544 1104 22-23 0.24061508045400615 1104 24-25 0.2423316205562287 1104 26-27 0.28225857629421824 1104 28-29 0.27715071681632253 1104 30-31 0.2804042793217789 1104 32-33 0.10992998772033502 1104 34-35 0.08321665138446832 1104 36-37 0.21556095637031802 1104 38-39 0.2288352507121445 1104 40-41 0.1847419531649379 1104 42-43 0.2937364506965139 1104 44-45 0.34267293061159165 1104 46-47 0.2776755235412196 1104 48-49 0.34880916376351934 1104 50-51 0.3651296909489261 1104 52-53 0.372720147840262 1104 54-55 0.36854181777624717 1104 56-57 0.3000832037483363 1104 58-59 0.3665014510274389 1104 60-61 0.49040814722474124 1104 62-63 0.4015280026791572 1104 64-65 0.35865659909903513 1104 66-67 0.38869195283590585 1104 68-69 0.2866754110708811 1104 70-71 0.30921672272202727 1104 72-73 0.44744169942930867 1104 74-75 0.47321273605576053 1104 76-77 0.341330914601798 1104 78-79 0.33859726751381203 1104 80-81 0.5688745704467095 1104 82-83 0.3646674267015264 1104 84-85 0.7509784440779477 1104 86-87 0.8298310898182883 1104 88-89 0.7258693086876136 1104 90-91 0.7954828056709253 1104 92-93 0.7225481433573862 1104 94-95 0.7525555396059183 1104 96-97 1.027459352386476 1104 98-99 1.1034030087599085 1104 100-101 1.669527130640251 1105 1 0.0425818132135376 1105 2 0.11646816405085758 1105 3 0.058509515877673834 1105 4 0.14277910256768678 1105 5 0.1626423279442193 1105 6 0.08155591295626863 1105 7 0.07272699118577464 1105 8 0.16992948360113047 1105 9 0.06583499258322689 1105 10-11 0.18005248146546649 1105 12-13 0.15366190121772405 1105 14-15 0.20127888174660313 1105 16-17 0.19336507276941717 1105 18-19 0.17030032326716338 1105 20-21 0.23071322581586884 1105 22-23 0.2639442482314891 1105 24-25 0.1751970786562893 1105 26-27 0.08296333280359391 1105 28-29 0.14529782203270258 1105 30-31 0.17942093043703977 1105 32-33 0.2589195904929298 1105 34-35 0.27722547125472374 1105 36-37 0.17800241642542147 1105 38-39 0.07711276559706448 1105 40-41 0.16276964578023723 1105 42-43 0.0958037882953775 1105 44-45 0.17568068409955373 1105 46-47 0.2388688719816301 1105 48-49 0.2195501883791806 1105 50-51 0.32741202677048165 1105 52-53 0.33919120000413017 1105 54-55 0.1493740958354337 1105 56-57 0.08022461048431495 1105 58-59 0.01303804127003616 1105 60-61 0.18531178403269877 1105 62-63 0.15627418147591499 1105 64-65 0.18814129206979402 1105 66-67 0.19732465078311634 1105 68-69 0.28164880705417517 1105 70-71 0.2672640767609451 1105 72-73 0.3339765679088238 1105 74-75 0.2324727111735072 1105 76-77 0.09495394845943395 1105 78-79 0.12345136197751572 1105 80-81 0.1812917036556314 1105 82-83 0.35394878500597926 1105 84-85 0.4274828206886312 1105 86-87 0.4468426645094823 1105 88-89 0.2296205305839969 1105 90-91 0.14878020414244375 1105 92-93 0.1080130193904445 1105 94-95 0.46812083330537746 1105 96-97 0.5762625048791428 1105 98-99 0.3423097447414243 1105 100-101 0.8922532370118859 1106 1 0.14997486932717408 1106 2 0.14937918804061212 1106 3 0.10399740154355186 1106 4 0.15170472240239974 1106 5 0.10092764196122772 1106 6 -0.0027953647231626633 1106 7 0.03708011506583375 1106 8 -0.005139423122301423 1106 9 0.06710463561490343 1106 10-11 0.06822832664525436 1106 12-13 0.16142544635732037 1106 14-15 0.21739860242730202 1106 16-17 0.13340253978216055 1106 18-19 0.2013579790267599 1106 20-21 0.03912701367655558 1106 22-23 0.060510423663984625 1106 24-25 -0.034090885201614185 1106 26-27 0.002289741074577023 1106 28-29 -0.14927624862247768 1106 30-31 0.04006346255550142 1106 32-33 -0.004372076626232513 1106 34-35 -0.024890871964835526 1106 36-37 -0.16971882899979818 1106 38-39 0.14165930614289124 1106 40-41 0.24551923581510238 1106 42-43 0.24906678500479273 1106 44-45 0.24788086654761088 1106 46-47 0.21647766206861974 1106 48-49 0.06510236526692381 1106 50-51 0.23189319009407683 1106 52-53 0.026650230640200334 1106 54-55 0.17789376873212603 1106 56-57 -0.032239415753451794 1106 58-59 0.10983444878081627 1106 60-61 0.08871916449121642 1106 62-63 0.21238233625268776 1106 64-65 0.17658548794082662 1106 66-67 0.20075614044982615 1106 68-69 0.13388254691364665 1106 70-71 0.2253135657977694 1106 72-73 0.33126281588615214 1106 74-75 0.2651840739953748 1106 76-77 0.08522159325043788 1106 78-79 0.04034903902272191 1106 80-81 0.22504464943163427 1106 82-83 0.23053722344604566 1106 84-85 0.5221036436597117 1106 86-87 0.527133115308235 1106 88-89 0.5050744787383401 1106 90-91 0.4911455672466687 1106 92-93 0.441708787247002 1106 94-95 0.30481814328246415 1106 96-97 0.6186176595798969 1106 98-99 0.6596435595349206 1106 100-101 0.9152782910404049 1107 1 0.10136365620721932 1107 2 0.11860641893206747 1107 3 0.1082380899080988 1107 4 0.1319027422043817 1107 5 0.021000891073263972 1107 6 -0.025104489154323062 1107 7 0.05421997919425081 1107 8 0.08664629311198979 1107 9 -0.022254097850748167 1107 10-11 0.05780922488173701 1107 12-13 0.025537651842633124 1107 14-15 0.13681744440052057 1107 16-17 0.15570683773770355 1107 18-19 -0.009099440756166643 1107 20-21 0.03331583211755884 1107 22-23 0.0052952279042415284 1107 24-25 0.08927881527569781 1107 26-27 0.0994711947334821 1107 28-29 0.1089054144635071 1107 30-31 0.08387993938778493 1107 32-33 0.13627939685479618 1107 34-35 0.06753010289090611 1107 36-37 0.07175373290603915 1107 38-39 -0.06264677047384737 1107 40-41 0.020731852558107278 1107 42-43 0.08863358153863032 1107 44-45 0.11287747122694469 1107 46-47 -0.1247418351301306 1107 48-49 -0.054513124110428635 1107 50-51 0.07381069009722552 1107 52-53 0.18639455963627682 1107 54-55 0.05601686568426345 1107 56-57 -0.06072737327711053 1107 58-59 -0.05481961253052958 1107 60-61 0.1795466679316533 1107 62-63 0.15435923338286983 1107 64-65 0.0037014463256994645 1107 66-67 -0.05281036271823325 1107 68-69 0.08143780061627837 1107 70-71 -0.02447758420866819 1107 72-73 0.20240383878840973 1107 74-75 0.23224206640815126 1107 76-77 0.04398182485411439 1107 78-79 0.05776479918633726 1107 80-81 0.4028200617700364 1107 82-83 0.4795019444274935 1107 84-85 0.528362533293322 1107 86-87 0.34637599027607635 1107 88-89 0.354216215958985 1107 90-91 0.34580784825583066 1107 92-93 0.34594122863365584 1107 94-95 0.3873933177385709 1107 96-97 0.5952741479789374 1107 98-99 0.5822902119963622 1107 100-101 1.045111009592663 1108 1 0.09567607507101172 1108 2 0.12251777218292403 1108 3 0.05852692036653906 1108 4 0.1171439988881886 1108 5 0.07567060223042432 1108 6 0.010847774489143092 1108 7 0.04003378000418678 1108 8 0.10227695407756698 1108 9 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-1.2062616034539246 2316 80-81 -0.9317742986671185 2316 82-83 -1.0341031980667346 2316 84-85 -0.8640683671938376 2316 86-87 -1.1853130518205681 2316 88-89 -1.2522313587206177 2316 90-91 -1.3552112525419027 2316 92-93 -1.4319638143734252 2316 94-95 -1.466287155981771 2316 96-97 -1.7225927888816024 2316 98-99 -2.2141025529753513 2316 100-101 -3.346793086267425 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 16.0 4 43.0 5 82.0 6 164.0 7 401.0 8 1003.0 9 1858.0 10 2999.0 11 4483.0 12 6399.0 13 8547.0 14 10989.0 15 14037.0 16 17337.0 17 20373.0 18 19575.0 19 15753.0 20 15743.0 21 16594.0 22 17957.0 23 20613.0 24 24881.0 25 30064.0 26 36802.0 27 46286.0 28 56893.0 29 70835.0 30 87656.0 31 107784.0 32 135261.0 33 171027.0 34 225281.0 35 304525.0 36 431756.0 37 552266.0 38 325579.0 39 17789.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.0362594241939 18.612565686463 14.6815585543338 23.669616335009298 2 16.104072626098603 19.89843441297758 38.68923853121846 25.30825442970536 3 20.315361345038554 22.861533910601985 29.02199038153573 27.801114362823736 4 13.595117411652218 16.14787924183552 35.87346949197986 34.3835338545324 5 14.535456138235315 36.80710328635167 33.76189023741462 14.895550337998397 6 33.912199094072605 34.87777924367972 16.58137245305449 14.628649209193192 7 30.091089254986077 31.50715052779563 19.69838831174911 18.703371905469186 8 27.107919700729028 33.96958206189984 19.07480072008886 19.847697517282274 9 26.186954985934435 16.714367588624413 19.695657478298738 37.40301994714241 10-11 25.869380334878205 25.733477039010488 26.548737220054107 21.8484054060572 12-13 26.723018161821958 22.809712005875962 27.01931658606193 23.447953246240143 14-15 23.252924751768045 24.692044129022097 25.279618930211328 26.775412188998526 16-17 23.20596655190073 27.574324774830366 26.020586937678836 23.19912173559007 18-19 23.122753233782852 26.589174807278177 27.69952418917807 22.588547769760904 20-21 23.877631686043472 25.533669675135386 27.617916611325903 22.970782027495243 22-23 23.425815260759315 25.41661199084993 27.75879985104534 23.398772897345417 24-25 23.593851297961592 25.56384617676224 27.803324665597028 23.03897785967914 26-27 23.239730962267934 25.705271785457857 27.906236495224775 23.148760757049438 28-29 22.98636328089187 26.349729115199498 27.6705396753 22.99336792860863 30-31 23.272482557381757 25.650707856869897 27.535991332111532 23.540818253636818 32-33 23.254803093303106 25.645310087568866 27.651300054870198 23.44858676425783 34-35 23.868186509386117 25.702974545944624 27.35443805659688 23.07440088807238 36-37 23.734318573352592 25.477604675102206 27.612539644118396 23.175537107426806 38-39 23.371440285316254 25.76333305906353 27.669183092117773 23.196043563502442 40-41 23.566053899424414 25.782630799919353 27.43725662899224 23.214058671663995 42-43 23.398243183259858 25.79163244781962 27.051069268404447 23.759055100516072 44-45 23.560417485712037 25.630432188185992 27.226566524571716 23.582583801530255 46-47 24.02245108110375 26.05411007037597 26.85406992342821 23.069368925092075 48-49 23.946575155663457 25.990003278805386 26.74313547779146 23.320286087739696 50-51 23.475966410694955 26.2066623576174 27.115499366498824 23.20187186518882 52-53 24.00031351447779 26.260224464307846 26.438491491752604 23.300970529461758 54-55 23.564153648500195 26.06404946770262 26.671826941065586 23.699969942731595 56-57 23.768488588587203 26.15948842932437 26.51185302132951 23.560169960758913 58-59 24.171765255947587 25.855851733130137 26.465110537131814 23.507272473790465 60-61 23.69344140495233 26.05579207085775 27.090322595520878 23.160443928669043 62-63 23.400710184597628 26.107885489384298 27.062034209601286 23.429370116416784 64-65 23.369832799362072 26.40454778924547 26.697759023357577 23.52786038803488 66-67 23.52468949931532 26.234936030776446 26.38116457219019 23.85920989771805 68-69 23.574869649549115 26.008361793233497 26.707731440211337 23.709037117006055 70-71 23.733428569756633 25.94308264200923 26.564897516517565 23.758591271716572 72-73 23.66902434573918 25.875272174593206 26.83922108813802 23.61648239152959 74-75 23.5055462644442 26.52973355622543 26.317735438052303 23.646984741278064 76-77 23.738115930952215 26.092931810673452 26.404626782643607 23.764325475730725 78-79 23.676614055830964 26.136153038138758 26.514821947403117 23.672410958627157 80-81 23.900988831154503 26.09275925447384 26.340476951009645 23.665774963362008 82-83 23.805985052798334 26.13215173655069 26.522397213601533 23.539465997049437 84-85 23.68971255155307 26.273472378586987 26.385824415442144 23.6509906544178 86-87 23.60045620921779 26.35060469357776 26.257302069771775 23.791637027432675 88-89 23.576966935152537 26.377228916432088 25.956050724457715 24.089753423957664 90-91 23.674144548008723 26.04804500176254 26.28156580104482 23.99624464918392 92-93 23.76021678501662 26.045065868652216 26.47551049114535 23.719206855185814 94-95 23.473467880378504 25.988393605627376 26.62366596175453 23.914472552239594 96-97 23.31922068668931 25.88413980745676 26.866406980721546 23.930232525132386 98-99 23.175396028994104 26.182941905339103 26.311036580432813 24.330625485233984 100-101 23.12500055446191 25.877420448022963 26.457769075929345 24.53980992158578 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 113.0 1 81.5 2 71.5 3 88.0 4 87.5 5 96.5 6 165.0 7 340.0 8 532.5 9 679.5 10 694.0 11 675.5 12 756.0 13 875.5 14 1044.0 15 1219.5 16 1398.0 17 1629.0 18 1902.0 19 2115.0 20 2417.5 21 2887.5 22 3370.0 23 4184.0 24 5257.0 25 6599.5 26 8638.0 27 11347.0 28 14279.5 29 18174.0 30 22976.5 31 28068.0 32 34555.0 33 39459.0 34 43255.5 35 48468.0 36 55081.5 37 61369.0 38 65426.5 39 68861.5 40 73413.5 41 78294.0 42 81945.5 43 86755.0 44 91882.5 45 97440.0 46 103072.5 47 112636.5 48 127421.0 49 126944.5 50 113747.0 51 107148.0 52 107148.5 53 108502.0 54 108444.0 55 102917.5 56 93678.0 57 85809.0 58 80739.0 59 73557.5 60 62175.5 61 51736.0 62 42997.0 63 34020.5 64 24525.5 65 18301.0 66 14589.5 67 11747.5 68 9178.5 69 7134.5 70 5604.5 71 4046.0 72 3077.5 73 2622.5 74 2115.5 75 1555.0 76 1142.5 77 797.0 78 516.0 79 340.5 80 250.5 81 175.5 82 121.0 83 83.5 84 54.5 85 33.0 86 16.5 87 8.5 88 3.0 89 2.0 90 1.5 91 1.5 92 1.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02049898356251055 2 0.013831494099271823 3 0.010036699564343401 4 0.0 5 0.003972121382354986 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0010107630303314026 14-15 3.901190643384361E-4 16-17 0.0 18-19 0.0015604762573537443 20-21 1.7732684742656188E-5 22-23 7.093073897062475E-5 24-25 0.0 26-27 0.0016846050505523375 28-29 0.0033869427858473316 30-31 0.0017555357895229623 32-33 0.009611115130519652 34-35 0.002198852908089367 36-37 0.0012235552472432767 38-39 0.0015604762573537443 40-41 4.078517490810923E-4 42-43 0.0062951030836429465 44-45 0.0020747241148907736 46-47 3.7238637959577993E-4 48-49 9.752976608460903E-4 50-51 1.5959416268390567E-4 52-53 1.9505953216921804E-4 54-55 0.0012235552472432767 56-57 0.01464719759743401 58-59 0.026067046571704593 60-61 0.021119627528503517 62-63 0.013654167251845263 64-65 0.018583853610303683 66-67 0.0032982793621340505 68-69 0.0014718128336404634 70-71 8.866342371328092E-5 72-73 1.7732684742656188E-5 74-75 0.0018264665284935871 76-77 0.0024293778097438973 78-79 0.010728274269306993 80-81 0.018282397969678527 82-83 0.018761180457730245 84-85 0.029560385466007862 86-87 0.011756769984381051 88-89 0.04007586751840298 90-91 0.04516514803954531 92-93 0.029170266401669424 94-95 0.030535683126853952 96-97 0.048800348411789825 98-99 0.05050268614708482 100-101 0.0569041853391837 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2819652.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.2304076620138 #Duplication Level Percentage of deduplicated Percentage of total 1 75.49365698534099 40.940517942112784 2 13.977935321753513 15.1605826154391 3 4.544922328540241 7.394189720067787 4 1.9577188590960268 4.246715672655603 5 1.0707172596697683 2.9032716741322906 6 0.6251206865175276 2.0340329800682078 7 0.43885397961537276 1.6659461153047128 8 0.30399533274141327 1.31886326575331 9 0.22613397094333326 1.1037003687438252 >10 1.2167131010720125 12.048299110997434 >50 0.09333560786273863 3.454619134135326 >100 0.04624930873336157 4.432094535143214 >500 0.0030108996230089613 1.1444670364046812 >1k 0.001570904151079009 1.8412210670484306 >5k 6.545433962829203E-5 0.3114787619932135 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8775 0.31120861723361604 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4730 0.1677511976655275 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4553 0.16147382726662723 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4145 0.14700395651661977 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3576 0.12682416127947704 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3269 0.11593629284748613 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 7.093073897062475E-5 0.0 0.0 0.0 4 0.0 7.093073897062475E-5 0.0 0.0 3.5465369485312375E-5 5 0.0 1.7732684742656187E-4 0.0 0.0 3.5465369485312375E-5 6 0.0 1.7732684742656187E-4 0.0 0.0 3.5465369485312375E-5 7 0.0 1.7732684742656187E-4 0.0 3.5465369485312375E-5 3.5465369485312375E-5 8 0.0 1.7732684742656187E-4 0.0 3.5465369485312375E-5 3.5465369485312375E-5 9 0.0 1.7732684742656187E-4 0.0 3.5465369485312375E-5 3.5465369485312375E-5 10-11 0.0 1.7732684742656187E-4 0.0 3.5465369485312375E-5 3.5465369485312375E-5 12-13 0.0 1.7732684742656187E-4 0.0 3.5465369485312375E-5 3.5465369485312375E-5 14-15 0.0 1.7732684742656187E-4 0.0 3.5465369485312375E-5 3.5465369485312375E-5 16-17 0.0 1.7732684742656187E-4 0.0 3.5465369485312375E-5 3.5465369485312375E-5 18-19 0.0 1.7732684742656187E-4 0.0 3.5465369485312375E-5 3.5465369485312375E-5 20-21 0.0 2.1279221691187424E-4 0.0 3.5465369485312375E-5 3.5465369485312375E-5 22-23 0.0 2.1279221691187424E-4 0.0 1.241287931985933E-4 5.3198054227968566E-5 24-25 0.0 2.1279221691187424E-4 0.0 1.7732684742656187E-4 7.093073897062475E-5 26-27 0.0 2.1279221691187424E-4 0.0 2.305249016545304E-4 1.5959416268390567E-4 28-29 0.0 2.1279221691187424E-4 0.0 4.965151727943732E-4 1.7732684742656187E-4 30-31 0.0 2.482575863971866E-4 0.0 8.511688676474968E-4 1.7732684742656187E-4 32-33 0.0 2.482575863971866E-4 0.0 0.0012944859862139015 2.1279221691187424E-4 34-35 0.0 2.482575863971866E-4 0.0 0.0021279221691187424 2.305249016545304E-4 36-37 0.0 2.482575863971866E-4 0.0 0.0029081602977956145 2.482575863971866E-4 38-39 0.0 2.659902711398428E-4 0.0 0.004096250175553579 2.83722955882499E-4 40-41 0.0 2.83722955882499E-4 0.0 0.005798587910848573 2.83722955882499E-4 42-43 0.0 2.83722955882499E-4 0.0 0.008192500351107159 2.83722955882499E-4 44-45 0.0 2.83722955882499E-4 0.0 0.013866959468757137 2.83722955882499E-4 46-47 0.0 2.83722955882499E-4 0.0 0.026084779256447248 2.83722955882499E-4 48-49 0.0 2.83722955882499E-4 0.0 0.034968854312518 3.5465369485312374E-4 50-51 0.0 2.83722955882499E-4 0.0 0.04599858422245015 3.5465369485312374E-4 52-53 0.0 2.83722955882499E-4 0.0 0.060716712558854774 4.6104980330906087E-4 54-55 0.0 3.5465369485312374E-4 0.0 0.07745636695592223 5.851785965076541E-4 56-57 0.0 3.5465369485312374E-4 0.0 0.09754749876935168 6.029112812503103E-4 58-59 0.0 3.5465369485312374E-4 0.0 0.1245721103171597 6.383766507356227E-4 60-61 0.0 3.901190643384361E-4 0.0 0.15656187359291146 6.383766507356227E-4 62-63 0.0 3.901190643384361E-4 0.0 0.1943856901489971 6.383766507356227E-4 64-65 0.0 4.255844338237485E-4 0.0 0.22566614603504265 6.561093354782788E-4 66-67 0.0 4.255844338237485E-4 0.0 0.28077933021521806 6.738420202209351E-4 68-69 0.0 4.255844338237485E-4 0.0 0.35194059408749734 6.915747049635913E-4 70-71 0.0 4.4331711856640467E-4 0.0 0.3983647627437712 7.802381286768722E-4 72-73 0.0 4.610498033090608E-4 0.0 0.48039616236329874 8.51168867647497E-4 74-75 0.0 4.610498033090608E-4 0.0 0.5431344009828163 8.51168867647497E-4 76-77 0.0 4.610498033090608E-4 0.0 0.5996661999424042 8.51168867647497E-4 78-79 0.0 4.610498033090608E-4 0.0 0.6744271988174427 8.51168867647497E-4 80-81 0.0 4.610498033090608E-4 0.0 0.7558734198404626 8.51168867647497E-4 82-83 0.0 4.610498033090608E-4 0.0 0.8575171687853678 8.51168867647497E-4 84-85 0.0 4.965151727943732E-4 0.0 1.0170581334150457 8.51168867647497E-4 86-87 0.0 6.029112812503103E-4 0.0 1.2352233538039445 8.51168867647497E-4 88-89 0.0 6.029112812503103E-4 0.0 1.505664528814194 8.866342371328093E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGGT 985 0.0 24.105417 4 TACATGG 10240 0.0 21.568434 2 GTACATG 10560 0.0 21.414923 1 GTGTAGC 1765 0.0 21.264534 1 GAGTACT 7605 0.0 20.63841 12-13 GTACTTT 7765 0.0 20.18257 14-15 ACATGGG 10530 0.0 20.07124 3 AGTACTT 7925 0.0 18.906195 12-13 AGAGTAC 13385 0.0 18.768343 10-11 GTATCAA 18905 0.0 18.722036 1 TAAGGTG 1330 0.0 17.85251 5 GTATTAG 935 0.0 17.78402 1 CATGGGG 6415 0.0 17.248056 4 GGTAAGG 1160 0.0 17.196253 3 TCAACGC 21130 0.0 16.563377 4 ACTTTTT 9565 0.0 16.53315 16-17 CAACGCA 21265 0.0 16.502888 5 ATCAACG 21280 0.0 16.448957 3 AACGCAG 21530 0.0 16.277708 6 TATCAAC 21680 0.0 16.25587 2 >>END_MODULE