FastQCFastQC Report
Thu 26 May 2016
SRR936455_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936455_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2258877
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73560.3256485412884367No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60830.2692931044939587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34420.15237660129347458No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33130.14666579897887314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA132000.027.8190381
GGTATCA93500.027.2834431
GTACATG107600.026.313011
TACATGG109000.025.2777292
ACATGGG107950.024.5554583
TCAACGC153950.023.4823474
ATCAACG154450.023.3448123
CAACGCA155750.023.1820015
TATCAAC158100.022.8962
GAGTACT77100.022.70490312-13
AACGCAG159650.022.6469656
GTACTTT84550.020.76254814-15
CATGGGG45600.020.5228614
ACGCAGA177300.020.391137
CGCAGAG178900.020.1560968
CATGGGA62000.019.9213244
AGTACTT82750.019.43243412-13
GCAGAGT187650.018.9643149
AGAGTAC142050.018.67831610-11
GTGTAGC12250.018.2262921
Produced by FastQC (version 0.11.5)