##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936455_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2258877 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.42826103413333 31.0 31.0 34.0 30.0 34.0 2 31.505503398370074 31.0 31.0 34.0 28.0 34.0 3 31.515984270059857 31.0 31.0 34.0 28.0 34.0 4 35.159859965814874 37.0 35.0 37.0 32.0 37.0 5 35.172605679724924 37.0 35.0 37.0 32.0 37.0 6 35.166727094923715 37.0 35.0 37.0 32.0 37.0 7 35.077870109793494 37.0 35.0 37.0 32.0 37.0 8 35.03131290459817 37.0 35.0 37.0 32.0 37.0 9 36.536177932663 39.0 37.0 39.0 32.0 39.0 10-11 36.402347715258514 39.0 37.0 39.0 32.0 39.0 12-13 36.30467418102003 39.0 35.0 39.0 32.0 39.0 14-15 37.3926632570078 40.0 37.0 41.0 32.0 41.0 16-17 37.28195581255642 39.0 37.0 41.0 32.0 41.0 18-19 37.24306591284076 39.0 36.5 41.0 31.5 41.0 20-21 37.113017220503814 39.0 36.0 41.0 31.0 41.0 22-23 37.22851089280204 39.0 37.0 41.0 31.0 41.0 24-25 37.27610710986034 40.0 37.0 41.0 31.0 41.0 26-27 37.18156898317173 39.0 36.5 41.0 31.0 41.0 28-29 37.03492089210701 39.0 36.0 41.0 31.0 41.0 30-31 36.81417713315068 39.0 36.0 41.0 30.0 41.0 32-33 36.62781306817503 39.0 35.5 41.0 30.0 41.0 34-35 36.4387919306806 39.0 35.0 40.5 30.0 41.0 36-37 36.23188292235478 39.0 35.0 40.0 30.0 41.0 38-39 36.02111159660309 38.0 35.0 40.0 29.0 41.0 40-41 35.80105579011163 38.0 35.0 40.0 28.5 41.0 42-43 35.55029755936246 38.0 35.0 40.0 27.0 41.0 44-45 35.27312952409538 38.0 34.0 40.0 26.5 41.0 46-47 35.067070052951095 38.0 34.0 40.0 25.5 41.0 48-49 34.895796451068385 38.0 34.0 40.0 24.5 41.0 50-51 33.93574152111868 37.5 33.0 39.5 23.5 40.5 52-53 34.039994209512074 37.5 33.0 39.5 24.0 40.5 54-55 34.47154072576772 38.0 34.0 40.0 24.0 41.0 56-57 34.36923280904627 38.0 34.0 40.0 23.5 41.0 58-59 34.13928027068317 38.0 33.5 40.0 23.0 41.0 60-61 33.84393395479258 37.5 33.0 40.0 22.5 41.0 62-63 33.4742101938264 37.0 33.0 40.0 22.0 41.0 64-65 33.11264314081731 36.0 32.0 39.0 20.5 41.0 66-67 32.66277867276527 36.0 32.0 39.0 19.5 41.0 68-69 32.278468017514896 35.0 31.0 39.0 18.0 40.0 70-71 31.885249174700526 35.0 31.0 38.0 17.5 40.0 72-73 31.48372443475231 35.0 31.0 37.5 14.0 39.5 74-75 31.05204687993193 35.0 30.5 37.0 11.5 39.0 76-77 30.58282102124197 34.5 30.0 36.0 7.5 39.0 78-79 30.087759094452686 34.0 29.5 36.0 7.0 38.5 80-81 29.622721600157952 34.0 29.0 35.5 2.0 37.0 82-83 29.2112129168609 34.0 29.0 35.0 2.0 37.0 84-85 28.820436438106192 34.0 29.0 35.0 2.0 36.0 86-87 28.443106685313097 34.0 28.5 35.0 2.0 36.0 88-89 28.02564548667325 34.0 27.0 35.0 2.0 36.0 90-91 27.603202166386218 33.0 26.5 35.0 2.0 35.0 92-93 27.142651414840206 33.0 25.5 35.0 2.0 35.0 94-95 26.628090639729386 33.0 25.0 35.0 2.0 35.0 96-97 26.00098456002695 32.5 24.5 35.0 2.0 35.0 98-99 25.188166730636507 32.0 19.0 35.0 2.0 35.0 100-101 22.182982296070126 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.44178141385718206 1101 2 0.47205618445225994 1101 3 0.42690296490225776 1101 4 0.47031524337458563 1101 5 0.44017732281317024 1101 6 0.49830156553700533 1101 7 0.49652442319920453 1101 8 0.5446994753444301 1101 9 0.5329160018350265 1101 10-11 0.7746820760374078 1101 12-13 0.7579875800347295 1101 14-15 0.6939156157290967 1101 16-17 0.7811742370708288 1101 18-19 0.8479810809869619 1101 20-21 0.8420880023042443 1101 22-23 0.7492100803146329 1101 24-25 0.6998275442199571 1101 26-27 0.6563653927375555 1101 28-29 0.7428461028152284 1101 30-31 0.6157209087006592 1101 32-33 0.47443369116145107 1101 34-35 0.5637537260115124 1101 36-37 0.47643268443591325 1101 38-39 0.3930976416214378 1101 40-41 0.41325628589102337 1101 42-43 0.5452172331204466 1101 44-45 0.28220922774168855 1101 46-47 0.38227817007009435 1101 48-49 0.6437861750546929 1101 50-51 0.5149080440446809 1101 52-53 0.6953242842888585 1101 54-55 0.4921219871208393 1101 56-57 0.46624899126530295 1101 58-59 0.4884360749738832 1101 60-61 0.5430978829590742 1101 62-63 0.5094082750458426 1101 64-65 0.49273747600036444 1101 66-67 0.5107018566341921 1101 68-69 0.5288134580561348 1101 70-71 0.4663474518970112 1101 72-73 0.3828673672094851 1101 74-75 0.29615799955347555 1101 76-77 0.33202503869813427 1101 78-79 0.3997671339817437 1101 80-81 0.522842199056111 1101 82-83 0.3308958092534482 1101 84-85 0.23245289684351533 1101 86-87 0.41782001262703616 1101 88-89 0.33574524396957983 1101 90-91 0.3451506658399026 1101 92-93 0.22012476255296676 1101 94-95 0.21381774452262547 1101 96-97 0.25294805871116566 1101 98-99 0.12484137308930698 1101 100-101 0.404799356046297 1102 1 0.37143355749856255 1102 2 0.3640039790457976 1102 3 0.3674785904389175 1102 4 0.3477671971996159 1102 5 0.41347661811391134 1102 6 0.4365498716052514 1102 7 0.47240665360035194 1102 8 0.469244870767902 1102 9 0.38809496581617964 1102 10-11 0.5443661316385047 1102 12-13 0.5439134876701885 1102 14-15 0.48577876155416533 1102 16-17 0.33004151204472976 1102 18-19 0.2572770911921154 1102 20-21 0.3549530327294619 1102 22-23 0.3008947786064624 1102 24-25 0.4072478921284812 1102 26-27 0.0846154759430533 1102 28-29 -0.09994011105124656 1102 30-31 0.43827949249963893 1102 32-33 0.2452061129126406 1102 34-35 0.387175403816876 1102 36-37 0.1903162822937574 1102 38-39 0.11818085581238336 1102 40-41 0.099369853655773 1102 42-43 0.1410583146214961 1102 44-45 0.21423251067411542 1102 46-47 0.17549051432278873 1102 48-49 0.1292548610964488 1102 50-51 0.41904183570090225 1102 52-53 0.4741552061988017 1102 54-55 0.26220198611924417 1102 56-57 0.33204487227153834 1102 58-59 0.3127761432284686 1102 60-61 0.2719722943040992 1102 62-63 0.3336320121046441 1102 64-65 0.21930699945284005 1102 66-67 0.092721522692365 1102 68-69 0.3469820607358898 1102 70-71 0.47070865452161215 1102 72-73 0.25703326905795265 1102 74-75 0.2806251765935599 1102 76-77 0.38933406339060284 1102 78-79 0.41018234071218984 1102 80-81 0.5179556802245422 1102 82-83 0.534335769662583 1102 84-85 0.10894350983761925 1102 86-87 0.22216876485758874 1102 88-89 0.18889397644643324 1102 90-91 0.32936553112351064 1102 92-93 0.16261637505635917 1102 94-95 0.030471259446233745 1102 96-97 0.24511448291974247 1102 98-99 0.4756413109068909 1102 100-101 0.6909515791545786 1103 1 0.21215889183603664 1103 2 0.19631756662494482 1103 3 0.22448931798382077 1103 4 0.3862962552046767 1103 5 0.3655880440461061 1103 6 0.4494604126389703 1103 7 0.352498875352822 1103 8 0.23747946972683565 1103 9 0.2464493471479159 1103 10-11 0.3185410150795249 1103 12-13 -0.009114944568423766 1103 14-15 0.3022664677019691 1103 16-17 0.484695003855137 1103 18-19 0.5203190473825785 1103 20-21 0.5272056490551975 1103 22-23 0.046436959635776986 1103 24-25 0.3319478898590802 1103 26-27 0.28533159819151166 1103 28-29 0.3572010650534736 1103 30-31 0.25599435649103697 1103 32-33 0.14325568840847325 1103 34-35 0.44541236463440015 1103 36-37 0.4981613510944385 1103 38-39 0.47438939195595964 1103 40-41 0.37842579944565813 1103 42-43 0.44651765409117417 1103 44-45 0.424297054133568 1103 46-47 0.5034568532150416 1103 48-49 0.3606239573955534 1103 50-51 0.41184968211452855 1103 52-53 0.44922179632219894 1103 54-55 0.47864917924783157 1103 56-57 0.5452896036878698 1103 58-59 0.48825097346463764 1103 60-61 0.5539271063178006 1103 62-63 0.5985200843825638 1103 64-65 0.5879974119039559 1103 66-67 0.6275064044439418 1103 68-69 0.4683915103555947 1103 70-71 0.43520059016139 1103 72-73 0.23117999511009302 1103 74-75 0.20228963969939784 1103 76-77 0.4713830975921631 1103 78-79 0.3467712285457587 1103 80-81 0.368503740684762 1103 82-83 0.22178850097133207 1103 84-85 0.12101664172094573 1103 86-87 0.2030145960090728 1103 88-89 0.028189839999928523 1103 90-91 0.15455882949685318 1103 92-93 0.1592801867599185 1103 94-95 0.20902272568972435 1103 96-97 0.05826549645966139 1103 98-99 0.07495485898164134 1103 100-101 0.20144545975548311 1104 1 0.3385158649287412 1104 2 0.4104653073821183 1104 3 0.3109020455504705 1104 4 0.4395806442959298 1104 5 0.42486285753862063 1104 6 0.5381015994078595 1104 7 0.4915309625513018 1104 8 0.47389408902456154 1104 9 0.6040764628771385 1104 10-11 0.5721807916488828 1104 12-13 0.6606778936816866 1104 14-15 0.4950760838869357 1104 16-17 0.44792095565382795 1104 18-19 0.45539758861715285 1104 20-21 0.1950353003919716 1104 22-23 0.34393480134260557 1104 24-25 0.5150236038155782 1104 26-27 0.2985971903549256 1104 28-29 0.30985009046802503 1104 30-31 0.4530388706429065 1104 32-33 0.3804285479147751 1104 34-35 0.33456259903707775 1104 36-37 0.1837548731193479 1104 38-39 0.06749661183358313 1104 40-41 -0.08210692922163787 1104 42-43 0.17660804444128786 1104 44-45 0.2400330751008326 1104 46-47 0.12669926307405177 1104 48-49 0.3720106306660824 1104 50-51 0.23168792191036403 1104 52-53 0.23024257440838625 1104 54-55 0.03508674008418211 1104 56-57 0.07936483955234053 1104 58-59 0.1559747738491737 1104 60-61 0.11759615312611515 1104 62-63 0.15723623529454755 1104 64-65 0.2601551719567894 1104 66-67 0.09447849969088651 1104 68-69 0.1011047858363554 1104 70-71 0.08333336560603044 1104 72-73 -0.024734932023864786 1104 74-75 -0.021724424461243785 1104 76-77 -0.02142196124067297 1104 78-79 0.010981482416070776 1104 80-81 0.18274572404440548 1104 82-83 0.07116612144880463 1104 84-85 -0.051264080851531446 1104 86-87 0.13628507227816655 1104 88-89 0.1636217050059976 1104 90-91 -0.10946861436172739 1104 92-93 -0.35543214973433024 1104 94-95 -0.18861871560298127 1104 96-97 -0.15544301873781663 1104 98-99 0.051100216157664136 1104 100-101 0.16731218214222565 1105 1 0.3800413618290506 1105 2 0.3528856565506402 1105 3 0.4076218991138205 1105 4 0.2923408370120839 1105 5 0.3198190819596931 1105 6 0.4905637382809189 1105 7 0.5701613999617194 1105 8 0.5278903590344015 1105 9 0.5002430345672053 1105 10-11 0.5695251595364326 1105 12-13 0.3626126533908902 1105 14-15 0.2742146461138759 1105 16-17 0.44027154019163817 1105 18-19 0.44300538705953585 1105 20-21 0.4865938664179694 1105 22-23 0.1684264174989849 1105 24-25 0.3033613406095128 1105 26-27 0.277779787187896 1105 28-29 0.09039482444719482 1105 30-31 0.010106424909082534 1105 32-33 -0.009973634820212851 1105 34-35 0.13364581580822232 1105 36-37 0.14886521866149138 1105 38-39 0.07671797638831634 1105 40-41 -0.038051687376672305 1105 42-43 0.058340935701714614 1105 44-45 0.14264121891267223 1105 46-47 0.050375289253054234 1105 48-49 0.1559511235083022 1105 50-51 0.06341131551066326 1105 52-53 0.19622575491573002 1105 54-55 0.24982469100718419 1105 56-57 0.10021582989926259 1105 58-59 0.17396489628264789 1105 60-61 0.24424442886908082 1105 62-63 0.32744872385418944 1105 64-65 0.3877967302637799 1105 66-67 0.47265045914451065 1105 68-69 0.4599395956241281 1105 70-71 0.38646543488714613 1105 72-73 0.29022146508193813 1105 74-75 0.47776710535277545 1105 76-77 0.2523860824140307 1105 78-79 0.3448571321423657 1105 80-81 0.4339619425941983 1105 82-83 0.3921735641606432 1105 84-85 0.2963747144997946 1105 86-87 0.19925499059829122 1105 88-89 0.15906602281497229 1105 90-91 0.31262441090204973 1105 92-93 0.07507899706495991 1105 94-95 -0.08406942274694629 1105 96-97 0.10982628309782072 1105 98-99 0.27702856926340047 1105 100-101 0.32795914548125893 1106 1 0.47369548511403536 1106 2 0.3763638655160477 1106 3 0.35478876616761923 1106 4 0.4726357045434 1106 5 0.3008723508280653 1106 6 0.5018377740777353 1106 7 0.5371613061634903 1106 8 0.5620530392762291 1106 9 0.6160376334944928 1106 10-11 0.5841663813010314 1106 12-13 0.5701435428539341 1106 14-15 0.673443624651135 1106 16-17 0.568178986918511 1106 18-19 0.5277319652147838 1106 20-21 0.3058358914365229 1106 22-23 0.5670951647809659 1106 24-25 0.4393308306029553 1106 26-27 0.2467119369580857 1106 28-29 0.21759861389575264 1106 30-31 0.24498401821700355 1106 32-33 0.23139138526114067 1106 34-35 0.1504960711952208 1106 36-37 0.10768438419831483 1106 38-39 0.14556374651442638 1106 40-41 0.1063503488976636 1106 42-43 0.11212620596852929 1106 44-45 -4.2427783423448773E-4 1106 46-47 0.10145985262229118 1106 48-49 0.1690136357660279 1106 50-51 -0.13068016334636212 1106 52-53 -0.031082662196482147 1106 54-55 0.10084564212125002 1106 56-57 0.11323144304124355 1106 58-59 0.3269423079630869 1106 60-61 0.3876670410645602 1106 62-63 0.429478858667828 1106 64-65 0.4527917419032903 1106 66-67 0.4634064728902061 1106 68-69 0.526252131331411 1106 70-71 0.2854029567378653 1106 72-73 0.03550111171841763 1106 74-75 0.23845831304764076 1106 76-77 0.23932553139205126 1106 78-79 0.10527376683236156 1106 80-81 0.3094472631709735 1106 82-83 0.17971340709124206 1106 84-85 0.3252613294921609 1106 86-87 0.3889124565111608 1106 88-89 0.47826720296726677 1106 90-91 0.4014882585055055 1106 92-93 0.4330314669429356 1106 94-95 0.34598617402298615 1106 96-97 0.39431154398481283 1106 98-99 0.3114928534549861 1106 100-101 0.25339031889713226 1107 1 0.3421453514393953 1107 2 0.35662305549898576 1107 3 0.3203369577671431 1107 4 0.357969779137143 1107 5 0.4164694337104109 1107 6 0.5015182243187652 1107 7 0.5565340315139551 1107 8 0.5692606427616482 1107 9 0.7435810917526098 1107 10-11 0.6994079374998776 1107 12-13 0.6008613028627323 1107 14-15 0.3173280735576256 1107 16-17 0.526773534785292 1107 18-19 0.5045607893836745 1107 20-21 0.5260963813404089 1107 22-23 0.6135420531673148 1107 24-25 0.655445828466398 1107 26-27 0.6754270550387673 1107 28-29 0.672466156023539 1107 30-31 0.6972974532564251 1107 32-33 0.45860908264972267 1107 34-35 0.6332690639288998 1107 36-37 0.6313614431050993 1107 38-39 0.7505775891452515 1107 40-41 0.7277042488132537 1107 42-43 0.7454777314070924 1107 44-45 0.8203590484225103 1107 46-47 0.6316655354151024 1107 48-49 0.8319273322151375 1107 50-51 0.7599795991448488 1107 52-53 0.8780700031675508 1107 54-55 1.0066261496198976 1107 56-57 0.7770338713478964 1107 58-59 0.793679265394573 1107 60-61 0.9221241102417395 1107 62-63 1.127231454861132 1107 64-65 1.0359651306546667 1107 66-67 1.2103973086171962 1107 68-69 1.2671601515721633 1107 70-71 1.0387855513054198 1107 72-73 0.7889005068309167 1107 74-75 0.8976500948319845 1107 76-77 0.8084834473506355 1107 78-79 0.6979112928689624 1107 80-81 0.7070974099805838 1107 82-83 0.7557496188840034 1107 84-85 0.4986331106180586 1107 86-87 0.6762699955266065 1107 88-89 0.5146588803506482 1107 90-91 0.4993016251548248 1107 92-93 0.7087416463422116 1107 94-95 0.7500361405406828 1107 96-97 0.7956446587413559 1107 98-99 0.9075745760572858 1107 100-101 0.5557092522967224 1108 1 0.27701895772097984 1108 2 0.2365203614133371 1108 3 0.3273120559903653 1108 4 0.30874896800465024 1108 5 0.25309906597227894 1108 6 0.21355164779560454 1108 7 0.3363825911112954 1108 8 0.3107739158874878 1108 9 0.24989986984031987 1108 10-11 0.26676967580841193 1108 12-13 0.15971119758003027 1108 14-15 0.3720687696079992 1108 16-17 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-2.0846194939297433 2316 72-73 -1.8475203120412793 2316 74-75 -1.587642811191163 2316 76-77 -1.7118259941691818 2316 78-79 -2.6490260293696615 2316 80-81 -2.6775942675187103 2316 82-83 -2.4730498035369486 2316 84-85 -2.0247936703799283 2316 86-87 -1.8764445675754935 2316 88-89 -2.4443114931398604 2316 90-91 -2.6424467722103735 2316 92-93 -3.2466981261554615 2316 94-95 -2.982675215579544 2316 96-97 -3.366923839631685 2316 98-99 -4.519427002656091 2316 100-101 -5.389046254616925 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4441.0 3 1824.0 4 1897.0 5 2146.0 6 2492.0 7 2795.0 8 3710.0 9 4686.0 10 5914.0 11 7640.0 12 9547.0 13 11494.0 14 13180.0 15 15717.0 16 18921.0 17 23743.0 18 18981.0 19 14945.0 20 15369.0 21 16268.0 22 17831.0 23 20279.0 24 23312.0 25 26902.0 26 31617.0 27 37291.0 28 44313.0 29 53573.0 30 65096.0 31 80460.0 32 100904.0 33 127643.0 34 168131.0 35 226394.0 36 311558.0 37 395938.0 38 293839.0 39 38081.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.3138574474067 18.527808093934087 14.362803588940961 22.795530869718256 2 15.651721156259976 19.934987051868237 40.40782298272357 24.005468809148212 3 19.588390234960794 24.417069912647378 29.42555545667192 26.5689843957199 4 12.711027632842029 16.45236248224463 37.34547074305105 33.491139141862284 5 13.861146746718731 37.33084403176517 33.8467813038455 14.961227917670602 6 31.8593422869147 35.787426039499636 17.689664198622282 14.663567474963383 7 28.94606006057325 32.66058750115661 20.312136829480693 18.081215608789453 8 26.590936867331592 34.24273743165189 19.655518418344485 19.510807282672037 9 26.610976829213207 17.015263260915585 20.338009531898912 36.0357503779723 10-11 25.141546879480604 26.48227024166324 27.115707612608077 21.26047526624808 12-13 26.38846051740977 23.81363364529602 27.55109699877714 22.246808838517076 14-15 23.03876049317769 25.342953158924175 26.23811415284293 25.380172195055206 16-17 22.261488480245255 28.708751701277485 26.746130098822995 22.283629719654268 18-19 22.1443457464603 27.007921939417233 29.208490186523473 21.639242127598994 20-21 23.053933193425824 26.004747347057734 29.221497978900484 21.71982148061596 22-23 22.554285744645806 26.131362558465316 29.101604353839804 22.21274734304907 24-25 22.4746066681527 26.274237580249615 29.285068718660234 21.966087032937452 26-27 22.23974592330463 26.496500092635817 29.18324869939091 22.08050528466865 28-29 21.928693739718337 26.749564827817018 29.24047180987358 22.08126962259106 30-31 22.105627344560887 26.30368486888167 29.30768895331785 22.28299883323959 32-33 21.945056178307883 26.515665490322554 29.129889772800972 22.409388558568587 34-35 22.701236056677722 26.48851619632233 28.849003287916958 21.961244459082987 36-37 22.57336632918229 26.23683523177518 28.882914908781824 22.306883530260706 38-39 22.37254280447444 26.50258358581879 28.900656786826723 22.224216822880052 40-41 22.568087356168164 26.45325962012879 28.870181192198046 22.108471831505003 42-43 22.307720403092368 26.589287394232525 28.54212713679352 22.560865065881586 44-45 22.502479706910925 26.65253844292778 28.39502920863427 22.449952641527016 46-47 22.655614481555848 26.859328789594926 27.98961157168031 22.495445157168916 48-49 22.74789955214204 26.936908759648382 27.810733140802885 22.504458547406696 50-51 22.610993140382757 27.15265343382624 27.74469358612838 22.491659839662617 52-53 22.928481557923185 27.154084333199634 27.519410731883536 22.398023376993645 54-55 22.554464641867504 27.00699809054441 27.692684345696023 22.745852921892066 56-57 22.677421632604183 27.25952674873891 27.435699412558574 22.627352206098333 58-59 22.937359407422626 26.876630054857948 27.510810216124632 22.675200321594797 60-61 22.657109135244543 26.9187519811299 27.991572561151614 22.43256632247394 62-63 22.567926812802007 26.98653970024769 27.979339181403052 22.466194305547255 64-65 22.395825147967436 27.089611854837297 27.767277144628583 22.747285852566684 66-67 22.438955274270945 27.26362222505106 27.454170102778964 22.843252397899036 68-69 22.506870215484504 27.002659509684733 27.707817098366426 22.782653176464336 70-71 22.53165039134735 26.995393125698442 27.607428402104254 22.865528080849955 72-73 22.590886630584166 26.977039912721914 27.675193754516215 22.756879702177702 74-75 22.45755928614178 27.43313806541951 27.433603041742256 22.675699606696455 76-77 22.608560305081205 27.15617256288403 27.440481356640706 22.79478577539406 78-79 22.581699635906205 27.169192881124705 27.431809927092655 22.81729755587644 80-81 22.555878312775004 27.489721943658594 27.28195667521519 22.672443068351207 82-83 22.607077315082098 27.38493931866564 27.320629483446307 22.687353882805954 84-85 22.516402198398247 27.353033313592768 27.225822877289307 22.904741610719682 86-87 22.599017277130596 27.29838882864517 27.264692689886154 22.837901204338078 88-89 22.545990571520484 27.31938802778375 27.035289382322343 23.099332018373424 90-91 22.495074182872372 27.278685881892876 27.281298812533738 22.94494112270101 92-93 22.696692550489395 27.246738248661824 27.27306340709987 22.783505793748915 94-95 22.4592768580359 27.178448176050868 27.287831329008625 23.074443636904604 96-97 22.477290383112912 27.113614390694185 27.313374448379523 23.095720777813373 98-99 22.378778919767644 27.197304401939093 27.043722635981315 23.380194042311942 100-101 22.460465039011666 27.081816520569035 27.11041215591377 23.347306284505528 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 254.0 1 197.0 2 229.0 3 321.0 4 268.0 5 263.5 6 455.5 7 895.5 8 1414.5 9 1714.0 10 1694.5 11 1600.0 12 1694.0 13 1830.0 14 2033.5 15 2176.0 16 2300.5 17 2577.0 18 2902.5 19 3198.5 20 3603.5 21 4196.5 22 4887.0 23 5859.0 24 7146.0 25 8783.5 26 11196.5 27 14245.0 28 17236.5 29 20977.5 30 25650.5 31 30485.5 32 36027.0 33 40302.5 34 44199.5 35 48133.5 36 52801.5 37 57980.5 38 60929.5 39 62565.5 40 64562.0 41 68766.5 42 72969.0 43 76505.0 44 79665.5 45 83049.0 46 84642.5 47 86972.0 48 95343.0 49 95677.5 50 87249.5 51 81772.5 52 77564.5 53 75426.0 54 73940.5 55 69215.5 56 62831.0 57 56009.5 58 50366.0 59 44854.0 60 38227.5 61 31993.0 62 26729.5 63 21141.0 64 15714.5 65 12248.0 66 9305.0 67 7328.5 68 5613.5 69 3841.0 70 2965.5 71 2421.0 72 1994.5 73 1766.5 74 1434.5 75 1162.5 76 865.5 77 536.0 78 350.0 79 247.0 80 173.5 81 112.5 82 68.5 83 42.0 84 31.0 85 22.0 86 13.0 87 13.0 88 10.5 89 6.5 90 6.0 91 5.0 92 2.5 93 1.0 94 1.5 95 1.5 96 1.0 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012041381624586022 2 0.011067446346126858 3 0.014609029176887453 4 0.01792926308072551 5 0.030103454061465054 6 0.04798844735680606 7 0.005489453387678922 8 0.025897824449936848 9 0.03430908367299326 10-11 0.03893527624567429 12-13 0.028664686036468563 14-15 0.027624346079932638 16-17 0.028664686036468563 18-19 0.02339658157571218 20-21 0.052902393534486385 22-23 0.012085651409970529 24-25 0.0028996709426852368 26-27 0.0013944982396119842 28-29 4.4269785384507435E-5 30-31 0.002589782444993685 32-33 0.0018814658788415658 34-35 0.0 36-37 3.0988849769155206E-4 38-39 4.4269785384507435E-5 40-41 0.0014387680249964916 42-43 0.003962145791913415 44-45 0.0018371960934570584 46-47 7.747212442288801E-4 48-49 5.976421026908504E-4 50-51 0.0034309083672993262 52-53 0.0033202339038380575 54-55 0.0052238346753718775 56-57 5.976421026908504E-4 58-59 0.005932151241523996 60-61 0.0028775360499929833 62-63 5.312374246140893E-4 64-65 8.189910296133875E-4 66-67 0.017243081407265645 68-69 0.041702137832206006 70-71 0.05153003018756665 72-73 0.015450155099193097 74-75 0.030878175305693933 76-77 0.02625198273301291 78-79 0.053876328812945545 80-81 0.07802549674019436 82-83 0.04681529804411661 84-85 0.01834982604187833 86-87 0.020275561706104405 88-89 0.022422646297253015 90-91 0.03878033199682852 92-93 0.02572074530839882 94-95 0.013391610078813499 96-97 0.018482635398031855 98-99 0.04967069920141735 100-101 0.0681533345994492 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2258877.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.898522484654684 #Duplication Level Percentage of deduplicated Percentage of total 1 79.43069808558512 47.57781455251237 2 12.354186897935044 14.799950833711769 3 3.721081116298176 6.686617827354306 4 1.5247434545784158 3.653195203895812 5 0.7879848745936412 2.3599564864207507 6 0.4862840169113952 1.7476616472537243 7 0.3130246250033014 1.3124798777307596 8 0.214661238069796 1.028631279608599 9 0.1644735947692593 0.8866552782976616 >10 0.8807268993662684 9.802799890627995 >50 0.07727876220413069 3.178205917327865 >100 0.04136934368446752 4.5643633458928825 >500 0.002819350171141994 1.169903156763318 >1k 5.193539788944902E-4 0.6345252525076744 >5k 1.4838685111271148E-4 0.5972394500945957 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7356 0.3256485412884367 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6083 0.2692931044939587 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3442 0.15237660129347458 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3313 0.14666579897887314 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 8.853957076901487E-5 0.0 14-15 0.0 0.0 0.0 8.853957076901487E-5 0.0 16-17 0.0 0.0 0.0 1.328093561535223E-4 0.0 18-19 0.0 0.0 0.0 1.5494424884577603E-4 0.0 20-21 0.0 4.4269785384507435E-5 0.0 1.7707914153802974E-4 0.0 22-23 0.0 4.4269785384507435E-5 0.0 3.0988849769155206E-4 0.0 24-25 0.0 4.4269785384507435E-5 0.0 4.426978538450743E-4 0.0 26-27 0.0 4.4269785384507435E-5 0.0 6.197769953831041E-4 0.0 28-29 0.0 4.4269785384507435E-5 0.0 0.0010624748492281785 0.0 30-31 0.0 4.4269785384507435E-5 0.0 0.002811131371916222 0.0 32-33 0.0 4.4269785384507435E-5 0.0 0.005644397636524698 0.0 34-35 0.0 4.4269785384507435E-5 0.0 0.009031036218439516 0.0 36-37 0.0 4.4269785384507435E-5 0.0 0.014011387074196604 0.0 38-39 0.0 4.4269785384507435E-5 0.0 0.018305556256493823 0.0 40-41 0.0 4.4269785384507435E-5 0.0 0.022334106726484 0.0 42-43 0.0 4.4269785384507435E-5 0.0 0.027469401831086863 0.0 44-45 0.0 4.4269785384507435E-5 0.0 0.03253829225761296 0.0 46-47 0.0 4.4269785384507435E-5 0.0 0.04106022594413065 0.0 48-49 0.0 4.4269785384507435E-5 0.0 0.052371156109872294 0.0 50-51 0.0 4.4269785384507435E-5 0.0 0.06401410966599776 0.0 52-53 0.0 4.4269785384507435E-5 0.0 0.08152280978557044 0.0 54-55 0.0 4.4269785384507435E-5 0.0 0.11155985916895873 0.0 56-57 0.0 4.4269785384507435E-5 0.0 0.16010167884307114 0.0 58-59 0.0 4.4269785384507435E-5 0.0 0.20720473049218707 0.0 60-61 0.0 4.4269785384507435E-5 0.0 0.25709677862052693 0.0 62-63 0.0 4.4269785384507435E-5 0.0 0.31088456786270346 0.0 64-65 0.0 4.4269785384507435E-5 0.0 0.3757398034510069 0.0 66-67 0.0 4.4269785384507435E-5 0.0 0.46540825374732664 0.0 68-69 0.0 4.4269785384507435E-5 0.0 0.5738470930466777 0.0 70-71 0.0 4.4269785384507435E-5 0.0 0.6839681841906399 0.0 72-73 0.0 4.4269785384507435E-5 0.0 0.7993573797953585 0.0 74-75 0.0 4.4269785384507435E-5 0.0 0.9235341277989019 0.0 76-77 0.0 4.4269785384507435E-5 0.0 1.0607483275981826 0.0 78-79 0.0 4.4269785384507435E-5 0.0 1.1937126280005508 4.4269785384507435E-5 80-81 0.0 4.4269785384507435E-5 0.0 1.3404226967648083 4.4269785384507435E-5 82-83 0.0 4.4269785384507435E-5 0.0 1.500989208354417 4.4269785384507435E-5 84-85 0.0 4.4269785384507435E-5 0.0 1.6969936831443235 4.4269785384507435E-5 86-87 0.0 4.4269785384507435E-5 0.0 1.924651054484153 4.4269785384507435E-5 88-89 0.0 4.4269785384507435E-5 0.0 2.182323340314679 4.4269785384507435E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13200 0.0 27.819038 1 GGTATCA 9350 0.0 27.283443 1 GTACATG 10760 0.0 26.31301 1 TACATGG 10900 0.0 25.277729 2 ACATGGG 10795 0.0 24.555458 3 TCAACGC 15395 0.0 23.482347 4 ATCAACG 15445 0.0 23.344812 3 CAACGCA 15575 0.0 23.182001 5 TATCAAC 15810 0.0 22.896 2 GAGTACT 7710 0.0 22.704903 12-13 AACGCAG 15965 0.0 22.646965 6 GTACTTT 8455 0.0 20.762548 14-15 CATGGGG 4560 0.0 20.522861 4 ACGCAGA 17730 0.0 20.39113 7 CGCAGAG 17890 0.0 20.156096 8 CATGGGA 6200 0.0 19.921324 4 AGTACTT 8275 0.0 19.432434 12-13 GCAGAGT 18765 0.0 18.964314 9 AGAGTAC 14205 0.0 18.678316 10-11 GTGTAGC 1225 0.0 18.226292 1 >>END_MODULE