FastQCFastQC Report
Thu 26 May 2016
SRR936455_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936455_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2258877
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77730.34410904179377627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69610.30816197606155626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40450.17907128188033258No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38270.16942046866650995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96250.028.9252431
GTATCAA133450.027.3415281
TAAGGTG9900.023.0292825
GTACATG97800.022.4431231
ATCAACG163450.022.3192443
TCAACGC164000.022.2139574
CAACGCA166900.021.8564325
TATCAAC165950.021.842312
GAGTACT95300.021.50684412-13
AACGCAG171000.021.3309736
TAGCCCT14950.021.2866844
TACATGG101050.021.2027172
GTAAGGT9650.020.1804544
ACATGGG101800.020.0176333
GTACTTT102600.019.93055514-15
AGTACTT100200.019.24629412-13
ACGCAGA191450.018.9532437
CGCAGAG193200.018.7569838
GTATAGG6650.018.5751291
AGAGTAC157500.018.41149910-11
Produced by FastQC (version 0.11.5)