##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936455_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2258877 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.730617913237417 31.0 31.0 34.0 30.0 34.0 2 31.850399556947988 31.0 31.0 34.0 30.0 34.0 3 31.9694086929036 31.0 31.0 34.0 30.0 34.0 4 35.73872636712844 37.0 35.0 37.0 35.0 37.0 5 35.586182426046214 37.0 35.0 37.0 33.0 37.0 6 35.63196800888229 37.0 35.0 37.0 33.0 37.0 7 35.55518118073715 37.0 35.0 37.0 33.0 37.0 8 35.51171666274879 37.0 35.0 37.0 33.0 37.0 9 37.08915757697298 39.0 37.0 39.0 33.0 39.0 10-11 36.97541676682705 39.0 37.0 39.0 33.0 39.0 12-13 36.83760824515899 39.0 37.0 39.0 33.0 39.0 14-15 37.876058103207924 40.0 37.0 41.0 33.0 41.0 16-17 37.84922441549496 39.5 37.0 41.0 32.5 41.0 18-19 37.99862011078956 40.0 37.0 41.0 33.0 41.0 20-21 38.01101498665045 40.0 37.0 41.0 33.0 41.0 22-23 37.90971907722289 40.0 37.0 41.0 33.0 41.0 24-25 37.783060786399616 39.5 37.0 41.0 32.0 41.0 26-27 37.56776176834772 39.0 36.5 41.0 32.0 41.0 28-29 37.56342510017146 39.0 37.0 41.0 32.0 41.0 30-31 37.431762995506176 39.0 36.0 41.0 32.0 41.0 32-33 37.23357579894788 39.0 36.0 41.0 31.0 41.0 34-35 37.04597594291323 39.0 36.0 41.0 31.0 41.0 36-37 36.85245943006193 39.0 35.0 41.0 31.0 41.0 38-39 36.63782578688436 39.0 35.0 40.0 30.0 41.0 40-41 36.39785433204199 39.0 35.0 40.0 30.0 41.0 42-43 36.11531681450562 38.0 35.0 40.0 30.0 41.0 44-45 35.8249535056579 38.0 35.0 40.0 29.0 41.0 46-47 35.43622605391971 38.0 34.5 40.0 27.5 41.0 48-49 35.032631922853696 38.0 34.0 40.0 26.0 41.0 50-51 34.713743599142404 38.0 34.0 40.0 25.5 41.0 52-53 34.4213463150052 38.0 33.0 40.0 24.0 41.0 54-55 34.073769620922256 37.5 33.0 40.0 24.0 41.0 56-57 33.71920560526315 37.0 33.0 40.0 23.0 41.0 58-59 33.3275523634089 37.0 32.0 39.0 22.5 40.5 60-61 32.93272807682756 36.0 31.5 39.0 21.5 40.0 62-63 32.57737716573324 36.0 31.0 39.0 20.0 40.0 64-65 32.232520628613244 35.0 31.0 39.0 19.5 40.0 66-67 31.75544086729822 35.0 30.5 38.0 18.0 40.0 68-69 31.289296628368874 35.0 30.0 38.0 15.5 40.0 70-71 30.75657882213153 34.0 29.0 37.0 12.5 39.0 72-73 30.159499167063988 34.0 29.0 36.5 8.0 39.0 74-75 29.596339464255912 34.0 28.0 36.0 7.5 38.5 76-77 28.281825659387387 31.5 26.5 34.5 7.0 36.5 78-79 29.064522105453285 33.0 28.5 35.0 4.5 37.0 80-81 28.88791598657209 33.5 28.0 35.0 2.0 37.0 82-83 28.443455752570856 33.0 27.5 35.0 2.0 36.0 84-85 27.952208774537084 33.0 26.5 35.0 2.0 36.0 86-87 27.4744576619267 32.5 26.0 35.0 2.0 35.5 88-89 26.907733134650535 32.0 25.5 34.0 2.0 35.0 90-91 26.25012694360959 32.0 24.5 34.0 2.0 35.0 92-93 25.57949503226603 31.0 23.5 34.0 2.0 35.0 94-95 24.725943023900815 31.0 19.0 34.0 2.0 35.0 96-97 23.783359164753108 31.0 8.5 34.0 2.0 35.0 98-99 22.509975753438546 30.0 2.0 34.0 2.0 35.0 100-101 18.669776397741003 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2595462526634975 1101 2 0.3861899149087833 1101 3 0.3277382257190311 1101 4 0.2892082165307528 1101 5 0.2606035079313571 1101 6 0.2777884033474791 1101 7 0.22954403319926087 1101 8 0.20614168733298754 1101 9 0.3186591253601634 1101 10-11 0.3042365927006614 1101 12-13 0.4445250661946645 1101 14-15 0.4568608512055832 1101 16-17 0.451519000110018 1101 18-19 0.43896680663178955 1101 20-21 0.40954947723808033 1101 22-23 0.43568839154933414 1101 24-25 0.3644190171771129 1101 26-27 0.2735981240160612 1101 28-29 0.4563959352743794 1101 30-31 0.5078788996466628 1101 32-33 0.5286645093626348 1101 34-35 0.5295471229937689 1101 36-37 0.47402710600638187 1101 38-39 0.6531322077501329 1101 40-41 0.5652237042568586 1101 42-43 0.7368883131786816 1101 44-45 0.9096885879086472 1101 46-47 0.6859563287437567 1101 48-49 0.7812949040214292 1101 50-51 0.7755472297562349 1101 52-53 0.6883752327595332 1101 54-55 0.7456582824573204 1101 56-57 0.7293732633284833 1101 58-59 0.8521352531015154 1101 60-61 0.7692100782718683 1101 62-63 0.776330231744538 1101 64-65 0.7357471606518615 1101 66-67 0.7693359987788106 1101 68-69 0.6814076845654427 1101 70-71 0.38340322836923235 1101 72-73 0.4970252104994799 1101 74-75 0.546225216976012 1101 76-77 0.7834190914472465 1101 78-79 0.9128975094653811 1101 80-81 0.783807907030809 1101 82-83 0.6930778921759639 1101 84-85 0.692527248100042 1101 86-87 0.7597788407269341 1101 88-89 0.4429656742847321 1101 90-91 0.644657559745557 1101 92-93 0.6963839590705128 1101 94-95 0.7490988666753466 1101 96-97 0.7142912955219671 1101 98-99 0.642082487048615 1101 100-101 0.7432992448168427 1102 1 0.31819073861698755 1102 2 0.4483549410563512 1102 3 0.4071369621083676 1102 4 0.23644915008772927 1102 5 0.20763334123170551 1102 6 0.24554327386859853 1102 7 0.2873220343267562 1102 8 0.19090838677178112 1102 9 0.31492452881030886 1102 10-11 0.4220944398049511 1102 12-13 0.45170830017409 1102 14-15 0.5894859785352082 1102 16-17 0.5076314132572222 1102 18-19 0.5060332190507424 1102 20-21 0.498617353100002 1102 22-23 0.4569157917366411 1102 24-25 0.34975528736596573 1102 26-27 0.31467120887245414 1102 28-29 0.5242987498633198 1102 30-31 0.5944238318320103 1102 32-33 0.5760618205644121 1102 34-35 0.5198163401596005 1102 36-37 0.6413430131932216 1102 38-39 0.6062533441720248 1102 40-41 0.5126368740611085 1102 42-43 0.4701903524349831 1102 44-45 0.5231448033853212 1102 46-47 0.5167106974235693 1102 48-49 0.6739647693032964 1102 50-51 0.4826011952589013 1102 52-53 0.6221290509093578 1102 54-55 0.6238896900717421 1102 56-57 0.7516726841764907 1102 58-59 0.7503223296620476 1102 60-61 0.5813583242095817 1102 62-63 0.44563628920901976 1102 64-65 0.4864569722231522 1102 66-67 0.5305916940515907 1102 68-69 0.5281679390125475 1102 70-71 0.1793290977492994 1102 72-73 0.024747160509409838 1102 74-75 0.2767990375533138 1102 76-77 0.46506338508039846 1102 78-79 0.5359515702587743 1102 80-81 0.6631339407699883 1102 82-83 0.6136715047064847 1102 84-85 0.526103213414217 1102 86-87 0.6609538379708049 1102 88-89 0.572277449192736 1102 90-91 0.5912764952646903 1102 92-93 0.5348814816855416 1102 94-95 0.6226061670230223 1102 96-97 0.6703584455745997 1102 98-99 0.744170675484984 1102 100-101 0.622156562953279 1103 1 0.25628734956003996 1103 2 0.33468633587253294 1103 3 0.2855291499879158 1103 4 0.1669410806843885 1103 5 0.1991177063162084 1103 6 0.2122425806925179 1103 7 0.25719917565415074 1103 8 0.2231713751028508 1103 9 0.22134231217660982 1103 10-11 0.277561301920322 1103 12-13 0.26288300122246255 1103 14-15 0.43181762886918307 1103 16-17 0.45887517135246725 1103 18-19 0.40661695954552357 1103 20-21 0.22567904131617667 1103 22-23 0.1845684489116124 1103 24-25 0.3291805572356239 1103 26-27 0.33270185587881684 1103 28-29 0.34752397283202185 1103 30-31 0.4088076346933107 1103 32-33 0.2818916608405715 1103 34-35 0.2686073795538917 1103 36-37 0.4284694549986199 1103 38-39 0.27532711737140403 1103 40-41 0.48020863968068284 1103 42-43 0.6165321192564974 1103 44-45 0.36195795683637044 1103 46-47 0.38521880128819674 1103 48-49 0.46076651859159057 1103 50-51 0.41261646749041603 1103 52-53 0.4834863547258976 1103 54-55 0.45827423258982236 1103 56-57 0.46644119261656414 1103 58-59 0.23643930806323255 1103 60-61 0.07836187372161874 1103 62-63 0.17826498738786256 1103 64-65 0.21209263578354154 1103 66-67 -0.009047773698110717 1103 68-69 0.03768648128351515 1103 70-71 -0.07443218306728383 1103 72-73 0.17841875794112028 1103 74-75 0.33029479839537856 1103 76-77 0.13499751360080836 1103 78-79 0.060639044255218266 1103 80-81 0.37551209702416344 1103 82-83 0.13901760944692754 1103 84-85 0.2936532095898592 1103 86-87 0.34505079688875284 1103 88-89 0.42096720603897353 1103 90-91 0.38714559618665234 1103 92-93 0.3296022654527633 1103 94-95 0.4134379531008321 1103 96-97 0.6336205337097489 1103 98-99 0.7178479716359938 1103 100-101 0.5761946266999267 1104 1 0.3344485181683119 1104 2 0.38689828542178617 1104 3 0.2755767528206974 1104 4 0.26545521191549426 1104 5 0.16285442550420726 1104 6 0.14484209434566253 1104 7 0.1477594376073128 1104 8 0.16210921969886272 1104 9 0.3201591203791523 1104 10-11 0.2768054693694779 1104 12-13 0.25706696003088325 1104 14-15 0.3966868930545928 1104 16-17 0.33158557484150464 1104 18-19 0.33348702169748634 1104 20-21 0.3054764713135327 1104 22-23 0.27788952038808645 1104 24-25 0.29455895504259644 1104 26-27 0.31758967622627665 1104 28-29 0.3326340715803795 1104 30-31 0.283355510967219 1104 32-33 0.3288236169936809 1104 34-35 0.42626734261353505 1104 36-37 0.466767464553584 1104 38-39 0.47566315759991795 1104 40-41 0.4550075142726726 1104 42-43 0.4110834178438836 1104 44-45 0.5961165148557583 1104 46-47 0.6562860643660784 1104 48-49 0.48420996787083936 1104 50-51 0.5190226427885349 1104 52-53 0.47110187930292824 1104 54-55 0.4329061189208545 1104 56-57 0.513731545819951 1104 58-59 0.5764695413652632 1104 60-61 0.49382170195243447 1104 62-63 0.41283155740427446 1104 64-65 0.44616469010029647 1104 66-67 0.5917717757947365 1104 68-69 0.5124524225573026 1104 70-71 0.332073050148761 1104 72-73 0.4035928831874749 1104 74-75 0.6641482717856029 1104 76-77 0.46602800830568114 1104 78-79 0.5347879372485913 1104 80-81 0.5894187280574954 1104 82-83 0.6509399865617951 1104 84-85 0.37742404872736657 1104 86-87 0.49296206782247864 1104 88-89 0.7043846354172132 1104 90-91 0.8661021517227177 1104 92-93 0.782551130242144 1104 94-95 0.8105849646816665 1104 96-97 1.0601655620685193 1104 98-99 1.0277098236751065 1104 100-101 1.0014775264828941 1105 1 0.15596261787647236 1105 2 0.2660820047054884 1105 3 0.2613601370960197 1105 4 0.18301932112131425 1105 5 0.19406425471900945 1105 6 0.22226650160532557 1105 7 0.1994722974391152 1105 8 0.16349017948058986 1105 9 0.2708158404053975 1105 10-11 0.30493529374910366 1105 12-13 0.23540866857359788 1105 14-15 0.3554857844186259 1105 16-17 0.340106357448704 1105 18-19 0.25833143275621495 1105 20-21 0.20821084766262743 1105 22-23 0.10263826410668031 1105 24-25 0.1778073274890204 1105 26-27 0.19880952115538975 1105 28-29 0.2534720242302342 1105 30-31 0.30083837200781005 1105 32-33 0.33933614527081346 1105 34-35 0.23659527091484023 1105 36-37 0.4571034226948001 1105 38-39 0.42287723485906525 1105 40-41 0.41896009504595355 1105 42-43 0.5142191998466856 1105 44-45 0.4755767887337399 1105 46-47 0.5709225630105053 1105 48-49 0.5630637729788575 1105 50-51 0.7108264211304274 1105 52-53 0.7360615574185871 1105 54-55 0.665233736389645 1105 56-57 0.8930285462862031 1105 58-59 0.8476165133385294 1105 60-61 0.7033513950916728 1105 62-63 0.7385804047336961 1105 64-65 0.6184987832464444 1105 66-67 0.6262412883057458 1105 68-69 0.5831781301329784 1105 70-71 0.5735375029971941 1105 72-73 0.6833773726450723 1105 74-75 0.7269907487753606 1105 76-77 0.5830125012169738 1105 78-79 0.5989954870249647 1105 80-81 0.738316136059705 1105 82-83 0.7128426269113106 1105 84-85 0.677377251165673 1105 86-87 0.8150759879385241 1105 88-89 0.822796626644827 1105 90-91 0.6731292114198304 1105 92-93 0.7742429225369527 1105 94-95 0.9549854033008778 1105 96-97 0.9196253178923115 1105 98-99 0.8457312883869825 1105 100-101 0.7966662708922279 1106 1 0.10472047347835911 1106 2 0.18074863726370438 1106 3 0.21648996237549767 1106 4 0.18897687315801903 1106 5 0.21697012393016735 1106 6 0.1097296005283539 1106 7 0.16150891285784041 1106 8 0.14722607776919716 1106 9 0.27223338749637804 1106 10-11 0.14627481776571472 1106 12-13 0.26060304409055135 1106 14-15 0.26904755908116584 1106 16-17 0.23992379198180203 1106 18-19 0.3001830951166937 1106 20-21 0.16597572107765046 1106 22-23 0.2523487686558994 1106 24-25 0.18115072281445777 1106 26-27 0.16457896510242165 1106 28-29 0.34097758817077306 1106 30-31 0.18440594510648367 1106 32-33 0.2133080740182649 1106 34-35 0.22265207853466507 1106 36-37 0.21182079156913858 1106 38-39 0.16096622379940584 1106 40-41 0.18426276488470705 1106 42-43 0.32278314892277393 1106 44-45 0.25777831849762123 1106 46-47 0.24039405261168412 1106 48-49 0.34673443886102717 1106 50-51 0.17755465354538558 1106 52-53 0.23883841142590967 1106 54-55 0.3113720631997339 1106 56-57 0.3210757523779648 1106 58-59 0.13064031250799246 1106 60-61 0.18643437241807703 1106 62-63 0.1639350899553591 1106 64-65 0.10181941118296578 1106 66-67 -0.10601669093926702 1106 68-69 -0.010167396970444997 1106 70-71 0.029478086081109467 1106 72-73 0.10589679314254141 1106 74-75 -0.038903276277292065 1106 76-77 -0.14180489068056445 1106 78-79 -0.38260974180008844 1106 80-81 -0.31993469746548797 1106 82-83 -0.027693046261539678 1106 84-85 0.034741861945647656 1106 86-87 0.11584436599655845 1106 88-89 -0.05360194762205239 1106 90-91 0.17941915028043454 1106 92-93 0.0783124014451424 1106 94-95 0.11096870721567598 1106 96-97 0.23924696993498173 1106 98-99 0.5510154543928003 1106 100-101 0.13828906556789633 1107 1 0.17219112258692704 1107 2 0.24263590526769718 1107 3 0.07852134172060232 1107 4 0.13460708535738064 1107 5 0.12215870919870042 1107 6 0.23084676549239447 1107 7 0.10635696635348779 1107 8 0.12286172384179395 1107 9 0.10661327598827341 1107 10-11 0.21232683904097627 1107 12-13 0.2862006258981751 1107 14-15 0.2754454272108049 1107 16-17 0.3437347834752913 1107 18-19 0.33222979520117235 1107 20-21 0.2216052103444781 1107 22-23 0.25015570358144856 1107 24-25 0.39455438424716505 1107 26-27 0.26468289089763886 1107 28-29 0.3387899535754997 1107 30-31 0.25979864511696604 1107 32-33 0.22876873308157997 1107 34-35 0.25688225550379684 1107 36-37 0.3285566629047949 1107 38-39 0.31666408619310005 1107 40-41 0.29547558434537535 1107 42-43 0.28666825627486503 1107 44-45 0.18579663821912362 1107 46-47 0.20689860250472947 1107 48-49 0.08349357301185023 1107 50-51 0.15199610330683555 1107 52-53 0.06280561065288026 1107 54-55 0.10251905846470066 1107 56-57 0.2506862207126801 1107 58-59 0.19006222781763427 1107 60-61 0.12619941255441347 1107 62-63 0.09457480379626304 1107 64-65 0.19309100200450757 1107 66-67 0.15040434157810267 1107 68-69 0.33700390036763395 1107 70-71 0.12303794506514265 1107 72-73 0.21858907877352962 1107 74-75 0.26656991701077004 1107 76-77 0.14000889275803274 1107 78-79 0.22099896434412614 1107 80-81 0.3457788352546771 1107 82-83 0.38989773522853355 1107 84-85 0.4211024668059977 1107 86-87 0.6092846017065092 1107 88-89 0.5973778465831536 1107 90-91 0.5027787297984325 1107 92-93 0.4606032015685493 1107 94-95 0.5908074593187109 1107 96-97 0.7182277063283209 1107 98-99 0.9226393509448876 1107 100-101 0.6361335895914877 1108 1 0.04988939723706309 1108 2 0.21422020898820193 1108 3 -0.028495758916246672 1108 4 -0.03670984853213355 1108 5 0.09503905281268743 1108 6 -0.02816633168673377 1108 7 0.014332081695854981 1108 8 -0.011091910266173954 1108 9 0.0200926269342645 1108 10-11 -0.027003397976102406 1108 12-13 -0.04899082573740543 1108 14-15 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-2.5925256423544987 2316 96-97 -2.577645177934887 2316 98-99 -1.8495264435717793 2316 100-101 -1.5314044213021774 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 11.0 4 45.0 5 79.0 6 231.0 7 483.0 8 1127.0 9 2047.0 10 3262.0 11 5212.0 12 7254.0 13 10135.0 14 12893.0 15 16840.0 16 20934.0 17 25427.0 18 23018.0 19 17551.0 20 17136.0 21 18138.0 22 19813.0 23 22243.0 24 26130.0 25 31116.0 26 38147.0 27 46413.0 28 56574.0 29 68696.0 30 82676.0 31 100342.0 32 122070.0 33 152445.0 34 194026.0 35 253027.0 36 331918.0 37 368244.0 38 158709.0 39 4461.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.41279539542864 19.68179755382146 15.147578725994798 22.757828324755106 2 16.03389239192129 20.85914858160393 38.98417569923646 24.122783327238324 3 19.675373862827904 24.44519902974301 29.988360989081286 25.8910661183478 4 13.351120956724458 17.24974478434831 36.70501320569452 32.69412105323271 5 13.753503012701604 37.53728799302275 33.90433731632703 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26.37373753471863 29.508086772436176 21.950493918651507 28-29 21.812632795668815 26.624166150000804 29.659549479176466 21.90365157515391 30-31 22.08220103507758 26.17343802605247 29.602284687726954 22.142076251143 32-33 21.896175569795208 26.36721188386369 29.512939205611488 22.22367334072961 34-35 22.597985527174398 26.361730328741917 29.220635773715344 21.819648370368338 36-37 22.561404681548748 26.104164227184096 29.224761399236293 22.109669692030867 38-39 22.20435349965634 26.38705128815289 29.373095606766107 22.035499605424665 40-41 22.422528377504648 26.322566250331192 29.205283779473458 22.0496215926907 42-43 22.274800738784972 26.417140802994805 28.90423154498496 22.403826913235264 44-45 22.467587376287483 26.489143403585587 28.713079935274365 22.330189284852565 46-47 22.633237666327116 26.805377185211942 28.182920097021665 22.378465051439278 48-49 22.81019765064631 26.90445601580284 27.923189574798325 22.362156758752523 50-51 22.62174600968107 27.071393218295036 27.919504772799513 22.387355999224383 52-53 22.9778193523196 27.074817795340216 27.65480036177525 22.292562490564936 54-55 22.54100937015522 26.970605708862028 27.802015517465783 22.686369403516974 56-57 22.745204993036694 27.19532299622049 27.52277878736426 22.536693223378553 58-59 23.047695994067745 26.813106299147236 27.592334552618325 22.54686315416669 60-61 22.69646202775977 26.87235922663635 28.0887751415791 22.342403604024778 62-63 22.627655472441347 26.954437013501863 28.04329858296593 22.374608931090858 64-65 22.45550641953348 27.04274686350196 27.888502303647627 22.61324441331693 66-67 22.545953027982534 27.147774614179614 27.604377526583708 22.701894831254148 68-69 22.596673281440744 26.872798226517457 27.83763293648378 22.692895555558014 70-71 22.603667917344577 26.941682991887195 27.740390255780234 22.71425883498799 72-73 22.67127793612046 26.864407605052914 27.758748617637398 22.705565841189227 74-75 22.50491428773819 27.301192710915917 27.493735389955372 22.700157611390523 76-77 22.595772759539756 27.08192821168475 27.56299169112418 22.759307337651315 78-79 22.643514225522686 27.075875726262367 27.540471134453533 22.740138913761417 80-81 22.573479704677794 27.346389997259635 27.352322295569238 22.727808002493337 82-83 22.640632778606513 27.259679311953093 27.386295242229338 22.713392667211053 84-85 22.533842300579174 27.268300187376386 27.276645243262802 22.92121226878163 86-87 22.546488615161767 27.20271004017735 27.265507217248352 22.98529412741253 88-89 22.50078525009247 27.239224476162377 27.04450016147708 23.215490112268075 90-91 22.46008766104396 27.263735777216986 27.1823393987692 23.093837162969848 92-93 22.592491917236394 27.102879867753906 27.309514396682516 22.995113818327187 94-95 22.403478563445734 27.060156578739708 27.242726264778728 23.293638593035833 96-97 22.428081962093053 26.90743859715916 27.31257416583974 23.35190527490805 98-99 22.285152181819107 27.053419190782257 26.99155171379552 23.66987691360312 100-101 22.15740497456758 27.09850389327747 27.058461030369934 23.68563010178502 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 397.0 1 299.0 2 271.0 3 347.0 4 292.5 5 267.0 6 439.5 7 867.5 8 1372.0 9 1720.0 10 1705.0 11 1533.5 12 1587.5 13 1791.0 14 2094.0 15 2398.5 16 2619.5 17 2868.5 18 3176.5 19 3485.0 20 3873.0 21 4502.5 22 5263.5 23 6199.0 24 7483.0 25 9160.0 26 11569.0 27 14578.0 28 17748.0 29 21387.5 30 25702.5 31 30643.0 32 36082.5 33 40207.0 34 44025.5 35 47751.5 36 52273.5 37 57452.0 38 60862.0 39 62581.0 40 64526.5 41 69106.0 42 73214.5 43 76577.0 44 79319.0 45 82261.5 46 84041.5 47 86869.5 48 95927.5 49 96097.5 50 87475.0 51 82058.0 52 77699.0 53 75537.0 54 73869.5 55 68801.5 56 62180.5 57 55873.0 58 50630.5 59 44778.0 60 38025.5 61 31747.0 62 26268.0 63 20700.5 64 15317.0 65 11945.5 66 9189.0 67 7226.5 68 5427.0 69 3709.0 70 2828.5 71 2216.5 72 1902.5 73 1716.0 74 1410.5 75 1177.0 76 883.5 77 530.5 78 346.5 79 264.0 80 184.0 81 125.0 82 84.5 83 56.0 84 34.5 85 18.0 86 9.0 87 7.5 88 4.5 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011155985916895874 2 0.00880968729151698 3 0.0041613598261436985 4 1.3280935615352232E-4 5 0.004736867036142295 6 0.0 7 2.2134892692253716E-4 8 0.0017707914153802973 9 8.853957076901487E-5 10-11 0.0 12-13 0.0 14-15 7.525863515366264E-4 16-17 0.0038957411138366544 18-19 0.0018371960934570584 20-21 4.869676392295818E-4 22-23 3.984280684605669E-4 24-25 3.541582830760595E-4 26-27 1.3280935615352232E-4 28-29 1.1067446346126858E-4 30-31 5.53372317306343E-4 32-33 0.003674392186914117 34-35 0.001018205063843671 36-37 0.0013502284542274768 38-39 0.005290239353448639 40-41 6.640467807676116E-5 42-43 0.0014609029176887453 44-45 0.004958215963064833 46-47 0.0 48-49 0.0019036007715338198 50-51 0.0015494424884577602 52-53 7.08316566152119E-4 54-55 1.3280935615352232E-4 56-57 0.01113385102420362 58-59 0.0017486565226880436 60-61 0.0030546151915310125 62-63 1.7707914153802974E-4 64-65 0.0030103454061465055 66-67 9.518003857669099E-4 68-69 0.001970005449610581 70-71 0.004515518109219759 72-73 0.0027889964792239684 74-75 0.006950356305367667 76-77 0.0012174190980739545 78-79 0.006087095490369772 80-81 0.0024791079815324165 82-83 0.0034309083672993262 84-85 0.002412703303455655 86-87 7.747212442288801E-4 88-89 0.0018814658788415658 90-91 0.007835752013057815 92-93 0.0025676475523014313 94-95 5.976421026908504E-4 96-97 1.5494424884577603E-4 98-99 7.304514588443727E-4 100-101 0.002102814805764103 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2258877.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.12971916658303 #Duplication Level Percentage of deduplicated Percentage of total 1 78.2678830269207 45.496900601178716 2 12.990476541059436 15.102655063437401 3 3.9678033000219846 6.919418746155581 4 1.6163617090173925 3.758346088671969 5 0.8363503149268642 2.430840446579095 6 0.4912407665891007 1.7133412683000833 7 0.3263472645471525 1.3279332387235987 8 0.24667275705197383 1.1471214474782403 9 0.16983755467730655 0.888534842159792 >10 0.9549859359292683 10.348999166930938 >50 0.08270385443429222 3.320967134670451 >100 0.04536902537835835 4.846757173859875 >500 0.0028996553644797356 1.1330078397295362 >1k 9.156806414145436E-4 0.9116226411196513 >5k 1.5261344023575728E-4 0.6535543010051061 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7773 0.34410904179377627 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6961 0.30816197606155626 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4045 0.17907128188033258 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3827 0.16942046866650995 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.4269785384507435E-5 2.2134892692253717E-5 18-19 0.0 0.0 0.0 6.640467807676116E-5 4.4269785384507435E-5 20-21 0.0 4.4269785384507435E-5 0.0 8.853957076901487E-5 4.4269785384507435E-5 22-23 0.0 4.4269785384507435E-5 0.0 2.2134892692253716E-4 4.4269785384507435E-5 24-25 0.0 4.4269785384507435E-5 0.0 3.541582830760595E-4 4.4269785384507435E-5 26-27 0.0 4.4269785384507435E-5 0.0 5.53372317306343E-4 4.4269785384507435E-5 28-29 0.0 4.4269785384507435E-5 0.0 9.518003857669099E-4 4.4269785384507435E-5 30-31 0.0 4.4269785384507435E-5 0.0 0.0027004569084549536 4.4269785384507435E-5 32-33 0.0 4.4269785384507435E-5 0.0 0.005644397636524698 4.4269785384507435E-5 34-35 2.2134892692253717E-5 4.4269785384507435E-5 0.0 0.009053171111131771 4.4269785384507435E-5 36-37 4.4269785384507435E-5 4.4269785384507435E-5 0.0 0.014144196430350125 4.4269785384507435E-5 38-39 4.4269785384507435E-5 6.640467807676116E-5 0.0 0.01839409582726284 4.4269785384507435E-5 40-41 4.4269785384507435E-5 8.853957076901487E-5 0.0 0.022334106726484 4.4269785384507435E-5 42-43 4.4269785384507435E-5 8.853957076901487E-5 0.0 0.027425132045702356 4.4269785384507435E-5 44-45 4.4269785384507435E-5 8.853957076901487E-5 0.0 0.03240548290145944 4.4269785384507435E-5 46-47 4.4269785384507435E-5 8.853957076901487E-5 0.0 0.040838877017208106 4.4269785384507435E-5 48-49 4.4269785384507435E-5 8.853957076901487E-5 0.0 0.05214980718294976 4.4269785384507435E-5 50-51 4.4269785384507435E-5 8.853957076901487E-5 0.0 0.06383703052445971 4.4269785384507435E-5 52-53 4.4269785384507435E-5 1.328093561535223E-4 0.0 0.08127932596595565 4.4269785384507435E-5 54-55 4.4269785384507435E-5 1.328093561535223E-4 0.0 0.11129424045665169 4.4269785384507435E-5 56-57 4.4269785384507435E-5 1.328093561535223E-4 0.0 0.1599245997015331 4.4269785384507435E-5 58-59 4.4269785384507435E-5 1.328093561535223E-4 0.0 0.20724900027757154 4.4269785384507435E-5 60-61 4.4269785384507435E-5 1.7707914153802974E-4 0.0 0.2566762156593741 4.4269785384507435E-5 62-63 4.4269785384507435E-5 1.7707914153802974E-4 0.0 0.3102205210819359 4.4269785384507435E-5 64-65 4.4269785384507435E-5 1.7707914153802974E-4 0.0 0.3744338447821639 4.4269785384507435E-5 66-67 4.4269785384507435E-5 1.7707914153802974E-4 0.0 0.4632833040488703 4.4269785384507435E-5 68-69 4.4269785384507435E-5 1.7707914153802974E-4 0.0 0.5711023663528381 4.4269785384507435E-5 70-71 4.4269785384507435E-5 1.7707914153802974E-4 0.0 0.6807143549648786 4.4269785384507435E-5 72-73 4.4269785384507435E-5 1.7707914153802974E-4 0.0 0.796435573959981 4.4269785384507435E-5 74-75 4.4269785384507435E-5 1.7707914153802974E-4 0.0 0.9197490611485264 4.4269785384507435E-5 76-77 4.4269785384507435E-5 1.7707914153802974E-4 0.0 1.057029665625884 4.4269785384507435E-5 78-79 4.4269785384507435E-5 1.7707914153802974E-4 0.0 1.1903702592040204 4.4269785384507435E-5 80-81 4.4269785384507435E-5 1.7707914153802974E-4 0.0 1.335840773977512 4.4269785384507435E-5 82-83 4.4269785384507435E-5 1.7707914153802974E-4 0.0 1.4952562711471231 4.4269785384507435E-5 84-85 4.4269785384507435E-5 1.7707914153802974E-4 0.0 1.6887816379554974 4.4269785384507435E-5 86-87 4.4269785384507435E-5 1.7707914153802974E-4 0.0 1.9120341656495683 4.4269785384507435E-5 88-89 4.4269785384507435E-5 1.7707914153802974E-4 0.0 2.164991719336644 4.4269785384507435E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9625 0.0 28.925243 1 GTATCAA 13345 0.0 27.341528 1 TAAGGTG 990 0.0 23.029282 5 GTACATG 9780 0.0 22.443123 1 ATCAACG 16345 0.0 22.319244 3 TCAACGC 16400 0.0 22.213957 4 CAACGCA 16690 0.0 21.856432 5 TATCAAC 16595 0.0 21.84231 2 GAGTACT 9530 0.0 21.506844 12-13 AACGCAG 17100 0.0 21.330973 6 TAGCCCT 1495 0.0 21.286684 4 TACATGG 10105 0.0 21.202717 2 GTAAGGT 965 0.0 20.180454 4 ACATGGG 10180 0.0 20.017633 3 GTACTTT 10260 0.0 19.930555 14-15 AGTACTT 10020 0.0 19.246294 12-13 ACGCAGA 19145 0.0 18.953243 7 CGCAGAG 19320 0.0 18.756983 8 GTATAGG 665 0.0 18.575129 1 AGAGTAC 15750 0.0 18.411499 10-11 >>END_MODULE