FastQCFastQC Report
Thu 26 May 2016
SRR936454_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936454_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2286351
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77840.3404551619589468No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62460.273186400513307No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35850.15680007138011617No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32200.14083576843625498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA136900.029.0902231
GGTATCA96000.027.4744011
GTACATG106150.024.3548451
ATCAACG162450.024.3080313
TCAACGC163900.024.1225014
TATCAAC164650.024.012622
CAACGCA164850.023.9011945
GAGTACT81300.023.61266512-13
AACGCAG168750.023.3196246
TACATGG107850.023.2203312
ACATGGG108250.021.9487883
GTACTTT88500.021.52820414-15
ACGCAGA184950.021.0948417
AGTACTT85450.020.9644512-13
CGCAGAG186300.020.8909668
AGAGTAC144500.020.14307210-11
GCAGAGT195050.019.5639729
ACTTTTT104900.018.38876216-17
TATACCG1300.00753924318.2801885
TACTTTT99050.017.98802414-15
Produced by FastQC (version 0.11.5)