##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936454_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2286351 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.311270666664917 31.0 31.0 34.0 30.0 34.0 2 31.36479831836844 31.0 31.0 34.0 28.0 34.0 3 31.40332214957371 31.0 31.0 34.0 28.0 34.0 4 35.060348564153095 37.0 35.0 37.0 32.0 37.0 5 34.98108033280979 37.0 35.0 37.0 32.0 37.0 6 35.103203751305024 37.0 35.0 37.0 32.0 37.0 7 34.942334313497796 37.0 35.0 37.0 32.0 37.0 8 34.92702214139474 37.0 35.0 37.0 32.0 37.0 9 36.42490763666646 39.0 37.0 39.0 32.0 39.0 10-11 36.298001706649586 39.0 35.0 39.0 32.0 39.0 12-13 36.19369160728165 39.0 35.0 39.0 32.0 39.0 14-15 37.24933463846977 39.0 36.5 41.0 31.5 41.0 16-17 37.17596313951795 39.0 36.0 41.0 31.0 41.0 18-19 37.1361335158075 39.0 36.0 41.0 31.0 41.0 20-21 37.045330528864554 39.0 36.0 41.0 31.0 41.0 22-23 36.939646843376195 39.0 36.0 41.0 31.0 41.0 24-25 36.79352535984195 39.0 36.0 41.0 30.0 41.0 26-27 36.58694793581563 39.0 36.0 40.0 30.0 41.0 28-29 36.703508122768554 39.0 36.0 41.0 30.0 41.0 30-31 36.63719984376852 39.0 36.0 41.0 30.0 41.0 32-33 36.48490651697836 39.0 35.0 40.5 30.0 41.0 34-35 36.31233196477706 39.0 35.0 40.0 30.0 41.0 36-37 36.07744436440424 38.5 35.0 40.0 29.5 41.0 38-39 35.89289199252433 38.0 35.0 40.0 29.0 41.0 40-41 35.74072725491405 38.0 35.0 40.0 28.0 41.0 42-43 35.47507644276841 38.0 34.5 40.0 27.0 41.0 44-45 35.20313831953187 38.0 34.0 40.0 26.5 41.0 46-47 34.85356622845748 38.0 34.0 40.0 24.5 41.0 48-49 34.596183175724114 38.0 33.5 40.0 24.0 41.0 50-51 33.89684151733483 37.5 33.0 39.5 23.5 40.5 52-53 34.08654423577133 37.5 33.0 39.5 24.0 40.5 54-55 34.445501587464044 38.0 34.0 40.0 24.0 41.0 56-57 34.36863981077271 38.0 34.0 40.0 23.5 41.0 58-59 34.13950723226661 38.0 33.0 40.0 23.0 41.0 60-61 33.82226023038457 37.0 33.0 40.0 22.5 41.0 62-63 33.5005285715098 37.0 33.0 40.0 22.0 41.0 64-65 33.19307249849214 36.0 32.0 39.5 21.5 41.0 66-67 32.832746590527876 36.0 32.0 39.0 20.0 41.0 68-69 32.45264922140126 35.5 31.5 39.0 19.5 40.5 70-71 32.06073214480192 35.0 31.0 38.5 18.0 40.0 72-73 31.6518301433157 35.0 31.0 37.5 17.5 40.0 74-75 31.229937354325735 35.0 31.0 37.0 14.0 39.0 76-77 30.812958290306256 35.0 30.0 36.5 11.0 39.0 78-79 30.359750099612874 34.0 30.0 36.0 7.5 38.5 80-81 29.943337877692443 34.0 29.5 35.5 7.0 37.0 82-83 29.539475784776705 34.0 29.0 35.0 3.5 37.0 84-85 29.15716353263344 34.0 29.0 35.0 2.0 36.5 86-87 28.809203836156392 34.0 29.0 35.0 2.0 36.0 88-89 28.426796891640873 34.0 28.5 35.0 2.0 36.0 90-91 28.066577047881097 34.0 27.5 35.0 2.0 35.0 92-93 27.706390226172623 34.0 27.0 35.0 2.0 35.0 94-95 27.273246758699777 33.0 26.0 35.0 2.0 35.0 96-97 26.677032747815186 33.0 25.0 35.0 2.0 35.0 98-99 25.992312641409825 33.0 24.5 35.0 2.0 35.0 100-101 23.85902536399704 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28463971614299055 1101 2 0.3759337915397474 1101 3 0.4719826002554228 1101 4 0.42137844809117553 1101 5 0.37727346383551463 1101 6 0.27801887403980174 1101 7 0.3623644240590451 1101 8 0.4894861587458408 1101 9 0.6094882640928532 1101 10-11 0.4398728493985331 1101 12-13 0.5540930167672968 1101 14-15 0.6707419684498959 1101 16-17 0.7469460503674696 1101 18-19 0.8059365519115786 1101 20-21 0.8383499261731586 1101 22-23 0.762434378817126 1101 24-25 0.7279596889856279 1101 26-27 0.6564126933320367 1101 28-29 0.7607912414152764 1101 30-31 0.5677261031031904 1101 32-33 0.5427948903436857 1101 34-35 0.5113959403623554 1101 36-37 0.6019875939832033 1101 38-39 0.6143055322125761 1101 40-41 0.5951949260619642 1101 42-43 0.3632189257547651 1101 44-45 0.4332668965953488 1101 46-47 0.5556920572598258 1101 48-49 0.42522828446881533 1101 50-51 0.7002582512924889 1101 52-53 0.5957980608670539 1101 54-55 0.643499832511381 1101 56-57 0.49811496904272445 1101 58-59 0.6474327742106283 1101 60-61 0.585325839194546 1101 62-63 0.5387866560792602 1101 64-65 0.5002375355005881 1101 66-67 0.5406716376522525 1101 68-69 0.5776193944505934 1101 70-71 0.5961916109010943 1101 72-73 0.6076051170276848 1101 74-75 0.4673107696287033 1101 76-77 0.40030847269322933 1101 78-79 0.3069177766568778 1101 80-81 0.50055592008475 1101 82-83 0.4008726694434195 1101 84-85 0.4191747157136838 1101 86-87 0.3527476687776172 1101 88-89 0.38419550748040265 1101 90-91 0.39960984675163047 1101 92-93 0.22142604577667768 1101 94-95 0.13250002253765558 1101 96-97 0.20832512318485286 1101 98-99 0.09610411893313042 1101 100-101 0.5244505815361507 1102 1 0.23132608128439003 1102 2 0.18386036257011185 1102 3 0.350993609420744 1102 4 0.34459499761001666 1102 5 0.3226952485005228 1102 6 0.33460666280550555 1102 7 0.10823344950256342 1102 8 0.21147585869761087 1102 9 0.3435257793969697 1102 10-11 0.4041264879112205 1102 12-13 0.4838054603371802 1102 14-15 0.5008293235551591 1102 16-17 0.46344936809742165 1102 18-19 0.36175344709874935 1102 20-21 0.13678785602727572 1102 22-23 -0.2138411744237061 1102 24-25 0.05685342782474123 1102 26-27 0.21009733713194834 1102 28-29 0.14346622638241513 1102 30-31 0.2529384042196483 1102 32-33 0.1853045873273942 1102 34-35 0.12495311459025515 1102 36-37 0.037890024231771235 1102 38-39 -0.03924955596504276 1102 40-41 -0.16216487630927645 1102 42-43 0.08905723095791984 1102 44-45 0.012254612699315715 1102 46-47 0.1672369454916378 1102 48-49 0.2110149844282887 1102 50-51 0.23475298261551814 1102 52-53 0.1712091282737731 1102 54-55 0.09173909757766552 1102 56-57 0.22602882426663484 1102 58-59 0.12234145700988108 1102 60-61 -0.19261323413712006 1102 62-63 -0.0490511983130304 1102 64-65 0.1418658237423287 1102 66-67 -0.01283642689210751 1102 68-69 -0.016273168360172008 1102 70-71 0.033189042539198965 1102 72-73 0.0038076655091039413 1102 74-75 0.11780853412482983 1102 76-77 0.18842237873836964 1102 78-79 0.07458987755451219 1102 80-81 0.11311303271116913 1102 82-83 -0.03881868961833135 1102 84-85 -0.049217247532627795 1102 86-87 -0.17471443055897495 1102 88-89 -0.46585501623722436 1102 90-91 -0.475875089935343 1102 92-93 -0.41935084694643976 1102 94-95 -0.371868607746336 1102 96-97 -0.3925828336969097 1102 98-99 -0.3329880221874717 1102 100-101 -0.10218807045536238 1103 1 0.29947316697193216 1103 2 0.4218853797095683 1103 3 0.36871129732353936 1103 4 0.2734991357578167 1103 5 0.4438904024482113 1103 6 0.20169216891464714 1103 7 0.2036044269799433 1103 8 -0.048647592430540954 1103 9 0.5236092684175517 1103 10-11 0.4582734805307993 1103 12-13 0.5098068691241053 1103 14-15 0.5275552009349198 1103 16-17 0.6645972119360977 1103 18-19 0.6552953534700237 1103 20-21 0.5695153404253759 1103 22-23 0.40637508596097405 1103 24-25 0.5357210359561932 1103 26-27 0.5870601430403823 1103 28-29 0.6312839825070569 1103 30-31 0.5179100411692446 1103 32-33 0.5675390047009259 1103 34-35 0.40538638124727555 1103 36-37 0.5644910830226664 1103 38-39 0.5876885970121535 1103 40-41 0.5304219746655647 1103 42-43 0.4784245351065479 1103 44-45 0.6780562848652139 1103 46-47 0.5296981160749681 1103 48-49 0.6871591786251798 1103 50-51 0.059858620635900195 1103 52-53 0.3998150457500458 1103 54-55 0.6279741187170345 1103 56-57 0.6210097730525419 1103 58-59 0.48342155946528464 1103 60-61 0.5916919286654618 1103 62-63 0.5020520182603221 1103 64-65 0.6437407904587715 1103 66-67 0.576818598383241 1103 68-69 0.3942924647985038 1103 70-71 0.6424192511911926 1103 72-73 0.6052288166993556 1103 74-75 0.4521660350584469 1103 76-77 0.365078984321201 1103 78-79 0.4324976055386891 1103 80-81 0.5722946662689878 1103 82-83 0.7627851032540391 1103 84-85 0.5407988780882924 1103 86-87 0.4150874203511812 1103 88-89 0.5507338446024868 1103 90-91 0.60927134679633 1103 92-93 0.46580109994635777 1103 94-95 0.4586085134095086 1103 96-97 0.6494350847422901 1103 98-99 0.6241580436162835 1103 100-101 0.6281980658448596 1104 1 0.27241289274021696 1104 2 0.3151763520728217 1104 3 0.3028928007380358 1104 4 0.262005255921423 1104 5 0.3385067532515933 1104 6 0.33194198947387576 1104 7 0.45521800065162665 1104 8 0.47248068718857894 1104 9 0.5267733922622497 1104 10-11 0.5200074971076987 1104 12-13 0.44169600546287313 1104 14-15 0.4222444098531497 1104 16-17 0.22743360703131543 1104 18-19 0.39325061219501123 1104 20-21 0.42277393395396246 1104 22-23 0.47387530191275573 1104 24-25 0.34986686921781285 1104 26-27 0.2973894731932063 1104 28-29 0.31967613586019183 1104 30-31 0.4601118379864033 1104 32-33 0.12549243090909812 1104 34-35 0.22816217215940782 1104 36-37 0.21364926446445764 1104 38-39 0.20329901770588066 1104 40-41 0.15821379682160597 1104 42-43 0.1762389063983889 1104 44-45 0.03376492714891555 1104 46-47 0.27583839802358057 1104 48-49 0.09663629011318164 1104 50-51 0.049266902923477573 1104 52-53 0.2717860454338563 1104 54-55 0.42792851888002303 1104 56-57 0.30141441505693933 1104 58-59 0.29212245748048105 1104 60-61 0.2592680514630956 1104 62-63 0.09133189324268187 1104 64-65 0.24606095671623507 1104 66-67 0.21118174715851978 1104 68-69 0.37916223563063767 1104 70-71 0.23895649922628337 1104 72-73 0.005981066490736708 1104 74-75 -0.09510355586577646 1104 76-77 0.09849507594966411 1104 78-79 0.1022254604007351 1104 80-81 0.12087553935015194 1104 82-83 -0.004915853604938292 1104 84-85 0.23899393098131938 1104 86-87 0.13921717565445846 1104 88-89 0.08693809909022221 1104 90-91 0.06645503398734931 1104 92-93 0.003581433631609343 1104 94-95 0.1635024970666734 1104 96-97 0.2758383410492691 1104 98-99 0.21496449689472996 1104 100-101 0.3630652550902447 1105 1 0.10490702248064565 1105 2 0.18819898461507734 1105 3 0.2240978637728297 1105 4 0.2605577178416354 1105 5 0.37030596858502207 1105 6 0.27339944803599536 1105 7 0.3856983621980987 1105 8 -0.025496124004391163 1105 9 0.3453464834025155 1105 10-11 0.2337850796373928 1105 12-13 0.2275572994321351 1105 14-15 0.2443676141689437 1105 16-17 0.258217783349302 1105 18-19 0.12935440684358213 1105 20-21 -0.14075792758480787 1105 22-23 -0.3417475191699424 1105 24-25 -0.26860759387133015 1105 26-27 -0.14242514580387677 1105 28-29 -0.05255559776332319 1105 30-31 -0.07126825619409516 1105 32-33 -0.3472077378929086 1105 34-35 -0.2966914238231837 1105 36-37 -0.350951628378958 1105 38-39 -0.20576498991263037 1105 40-41 -0.414895768112693 1105 42-43 -0.10217473605922578 1105 44-45 -0.03761704474560901 1105 46-47 0.05144251886889606 1105 48-49 1.650466947893392E-4 1105 50-51 -0.015377888695120134 1105 52-53 0.12210644240695956 1105 54-55 0.02137154788269413 1105 56-57 0.20598080089962423 1105 58-59 0.15383226350557067 1105 60-61 0.14123890459894284 1105 62-63 0.037628631018954195 1105 64-65 0.0755398494160957 1105 66-67 -0.34096799508837705 1105 68-69 0.07097809404715605 1105 70-71 0.1881121847905618 1105 72-73 0.07376790493299268 1105 74-75 0.06280514493555245 1105 76-77 0.030951843666823464 1105 78-79 -0.1436120064669062 1105 80-81 -0.02480422837726337 1105 82-83 -0.018690616359297252 1105 84-85 -0.02023442467951142 1105 86-87 -0.047818602808607835 1105 88-89 -0.15607654836095364 1105 90-91 -0.09451264241523205 1105 92-93 -0.13980406234123777 1105 94-95 0.044675562059929064 1105 96-97 0.06842055352190357 1105 98-99 -0.13166960457104437 1105 100-101 0.12195515760292253 1106 1 0.057404132937243446 1106 2 0.018216841486363933 1106 3 -0.03233944961082358 1106 4 0.11007064601367489 1106 5 0.10688559198119663 1106 6 0.16699914108537683 1106 7 0.3557706768237452 1106 8 0.293100749205486 1106 9 0.3135336244274427 1106 10-11 0.3753833203110162 1106 12-13 0.2601690601272537 1106 14-15 0.16037877376298582 1106 16-17 0.1684579354558835 1106 18-19 0.08815480909844808 1106 20-21 0.13557818524424903 1106 22-23 0.14724235412104747 1106 24-25 0.08392335465931211 1106 26-27 0.0666594582526372 1106 28-29 -0.016622683962964402 1106 30-31 0.18136575905458585 1106 32-33 0.05366991338989635 1106 34-35 -0.11188817257991701 1106 36-37 -0.09290985800134166 1106 38-39 0.0323230892000268 1106 40-41 -0.015126329596597543 1106 42-43 0.09106054818060016 1106 44-45 0.014788246667379212 1106 46-47 -0.0010846751563420298 1106 48-49 -0.0907690742503462 1106 50-51 0.0892788703017473 1106 52-53 -0.017146578940177903 1106 54-55 0.2382518992410354 1106 56-57 0.005091553422900574 1106 58-59 0.03994029250746678 1106 60-61 -0.0257252973801414 1106 62-63 0.029438423022526194 1106 64-65 -0.05276316483391952 1106 66-67 0.005871867810391507 1106 68-69 -0.02896145366326408 1106 70-71 -0.0316120063907519 1106 72-73 0.2013661969340248 1106 74-75 0.04035668779964752 1106 76-77 -0.1505222543647342 1106 78-79 -0.041172781929105895 1106 80-81 0.016707848773549472 1106 82-83 0.10575571290670993 1106 84-85 0.17757636599081295 1106 86-87 0.16222688532144858 1106 88-89 0.18247274877028374 1106 90-91 0.4565982159312405 1106 92-93 0.45273126802988983 1106 94-95 0.0835655845172596 1106 96-97 0.2917397232344179 1106 98-99 0.3359663211981072 1106 100-101 0.5140482167422427 1107 1 0.47534111062575946 1107 2 0.5003685403495375 1107 3 0.526020614039755 1107 4 0.46607674372334174 1107 5 0.4518231074372707 1107 6 0.45181597767178516 1107 7 0.599051879038619 1107 8 0.6245805276459748 1107 9 0.73206761984531 1107 10-11 0.823859154821676 1107 12-13 0.7605751405676671 1107 14-15 0.8599559298595523 1107 16-17 0.8631396364921713 1107 18-19 0.9890638656857931 1107 20-21 0.9514839297831656 1107 22-23 0.8367179883011033 1107 24-25 0.9574665997875655 1107 26-27 0.9873739683485567 1107 28-29 0.9225824858312279 1107 30-31 0.8325794211324364 1107 32-33 0.8681931238315101 1107 34-35 0.7770802584592928 1107 36-37 0.9744986822243789 1107 38-39 0.9603319637195398 1107 40-41 0.979356178867782 1107 42-43 1.1093526836777912 1107 44-45 0.9595488425579148 1107 46-47 1.1528308308482451 1107 48-49 1.3639167381697561 1107 50-51 1.20389525525011 1107 52-53 1.1981202211477608 1107 54-55 1.4060984210663179 1107 56-57 1.3470904049089185 1107 58-59 1.2424110427031394 1107 60-61 1.2988059533819367 1107 62-63 1.4983828432912176 1107 64-65 1.1592203990241359 1107 66-67 1.1089845972219052 1107 68-69 1.2782437284145018 1107 70-71 1.3476556542697935 1107 72-73 1.3887550689932162 1107 74-75 1.1328179344846667 1107 76-77 1.1660036299214873 1107 78-79 1.385080083710836 1107 80-81 1.3463877226284886 1107 82-83 1.3766258179258308 1107 84-85 1.5203367086229918 1107 86-87 1.3398950261974 1107 88-89 1.3720304841781719 1107 90-91 1.4761583592805252 1107 92-93 1.516777541690896 1107 94-95 1.6330208713215058 1107 96-97 1.8318366442306377 1107 98-99 2.0459189409317737 1107 100-101 2.426421097577684 1108 1 0.19622887503303588 1108 2 0.08755590191357854 1108 3 -0.01905283730653906 1108 4 0.04741069459986136 1108 5 0.17197638718490538 1108 6 0.2535419878186147 1108 7 0.3952897045953421 1108 8 0.181922465107057 1108 9 0.34972486148961224 1108 10-11 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62-63 -2.0441624751791885 2316 64-65 -2.0112733200671826 2316 66-67 -2.741332418529229 2316 68-69 -2.3676389630200063 2316 70-71 -2.0574930874184076 2316 72-73 -1.9672773713612166 2316 74-75 -2.247866636287977 2316 76-77 -2.7438967622459565 2316 78-79 -2.9521396013435393 2316 80-81 -2.8058714628218198 2316 82-83 -2.523909995102848 2316 84-85 -2.7930727033527667 2316 86-87 -2.8620845855484838 2316 88-89 -2.8976897965451265 2316 90-91 -3.0937911549683257 2316 92-93 -3.421054531692949 2316 94-95 -3.4919132197583593 2316 96-97 -3.583448423756966 2316 98-99 -3.9040307402970456 2316 100-101 -4.518479473343952 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4794.0 3 1985.0 4 1865.0 5 2209.0 6 2493.0 7 2883.0 8 3646.0 9 4610.0 10 6018.0 11 7639.0 12 9318.0 13 11390.0 14 12937.0 15 15277.0 16 18552.0 17 22336.0 18 19621.0 19 14522.0 20 14799.0 21 15987.0 22 17453.0 23 20309.0 24 23020.0 25 26855.0 26 32125.0 27 37391.0 28 45190.0 29 54496.0 30 65865.0 31 81549.0 32 101146.0 33 129518.0 34 169007.0 35 226510.0 36 308970.0 37 398547.0 38 309907.0 39 45594.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.2724169742908 18.54283488381147 14.371744684871718 22.813003457026014 2 15.60324171397228 19.91113630577929 40.424888832900734 24.060733147347694 3 19.598374391282498 24.332472063404165 29.50656537887347 26.562588166439866 4 12.733829223014286 16.474703019659266 37.33173374565593 33.45973401167052 5 13.90753572264655 37.30072221991279 33.82938457061718 14.96235748682348 6 31.82765450727734 35.82478715890064 17.676730102519084 14.670828231302938 7 28.90420475703826 32.631315535271796 20.331814968291077 18.13266473939886 8 26.60677326793357 34.22126405228015 19.647315493348394 19.52464718643789 9 26.630919526503494 16.933217325727224 20.336325822147042 36.09953732562224 10-11 25.158174018001834 26.458549426276655 27.067332610331285 21.315943945390227 12-13 26.36288197789162 23.823090118746045 27.55285836520015 22.261169538162186 14-15 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28.928182290980935 22.23639669651398 40-41 22.522966417396347 26.4422490227545 28.874548227617613 22.160236332231538 42-43 22.33226488413446 26.538231156363533 28.533935636305348 22.59556832319666 44-45 22.500402939479038 26.618739649047914 28.3913234500717 22.48953396140135 46-47 22.689976321916955 26.84966024673582 27.993193343893374 22.46717008745385 48-49 22.770205726902304 26.89858690688841 27.810374871191062 22.520832495018222 50-51 22.588882831094026 27.160724669724434 27.713667937928815 22.536724561252726 52-53 22.946381318532318 27.121631610484926 27.467928951638655 22.464058119344102 54-55 22.51044324954167 26.985649867437818 27.725058834515636 22.77884804850488 56-57 22.722454488017398 27.261766697932703 27.394623656149136 22.62115515790077 58-59 22.94758407636445 26.909072936419747 27.495391570526955 22.647951416688848 60-61 22.713619489686014 26.906154493813794 27.980121045121294 22.400104971378898 62-63 22.54982019155657 27.040591832646495 27.93451666038292 22.475071315414013 64-65 22.418747709154722 27.114510334281896 27.726248035012425 22.74049392155096 66-67 22.459578689354913 27.229153380973752 27.455327267621747 22.855940662049587 68-69 22.49230874881326 27.044619645293732 27.690538213713882 22.772533392179124 70-71 22.51308213476644 27.019906540870544 27.587874371263617 22.879136953099405 72-73 22.598508525818243 26.979590917311953 27.702736541729806 22.71916401514 74-75 22.47333335374492 27.393043643328497 27.38928207925266 22.744340923673928 76-77 22.58554960118551 27.168592068499166 27.41888993279901 22.82696839751631 78-79 22.590804900447896 27.151668258526147 27.46108548919818 22.796441351827777 80-81 22.593043438317196 27.457977472053336 27.25015339083986 22.69882569878961 82-83 22.62798609434431 27.40704922966027 27.30283991699996 22.66212475899546 84-85 22.531299790150065 27.40644509400599 27.17999525404527 22.882259861798673 86-87 22.627148799344937 27.287895425991586 27.25080313771015 22.834152636953323 88-89 22.573196615814954 27.304975784290463 27.008854125090625 23.11297347480396 90-91 22.54051803063846 27.302422792543226 27.24352286729998 22.913536309518342 92-93 22.69004259354767 27.29265664841229 27.249590665377095 22.76771009266295 94-95 22.470413091483927 27.17020053245828 27.30428833842879 23.055098037629005 96-97 22.472030764628027 27.06887924481096 27.385684580332438 23.073405410228574 98-99 22.359409635744885 27.199440206523366 27.04800810904336 23.393142048688382 100-101 22.346923728884015 27.089173816723715 27.30661810359878 23.25728435079349 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 316.0 1 242.5 2 240.5 3 312.5 4 277.0 5 277.0 6 456.5 7 888.5 8 1448.0 9 1775.5 10 1709.5 11 1604.0 12 1730.0 13 1878.0 14 2052.5 15 2321.5 16 2512.5 17 2679.5 18 2878.5 19 3202.5 20 3612.0 21 4151.0 22 4897.0 23 5921.0 24 7233.0 25 8919.5 26 11338.5 27 14298.0 28 17437.5 29 21281.0 30 25872.0 31 30865.0 32 36420.5 33 40432.5 34 44325.5 35 48504.0 36 53126.0 37 58282.0 38 61363.5 39 62754.0 40 64975.5 41 69734.0 42 73668.0 43 77198.0 44 80533.0 45 84014.0 46 85746.0 47 88143.0 48 96377.5 49 96702.0 50 88762.5 51 83362.0 52 79069.5 53 76903.5 54 74898.5 55 69733.5 56 63384.5 57 56827.0 58 51631.5 59 45805.5 60 38642.0 61 32432.0 62 27163.5 63 21267.0 64 15902.0 65 12548.0 66 9498.5 67 7483.5 68 5702.0 69 4003.5 70 3051.5 71 2379.0 72 2032.0 73 1799.5 74 1435.5 75 1213.0 76 922.5 77 552.0 78 370.0 79 273.0 80 174.5 81 116.5 82 74.5 83 45.5 84 32.5 85 21.5 86 15.5 87 12.5 88 10.5 89 5.0 90 5.5 91 5.5 92 2.5 93 3.0 94 3.5 95 2.0 96 1.5 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012334064192243447 2 0.006691885891536339 3 0.008397660726633836 4 0.03332821600882804 5 0.04531237767079508 6 0.05742775278161577 7 0.02444943930306414 8 0.022393761937690233 9 0.012858917987658064 10-11 0.042535026336726074 12-13 0.04056682460392127 14-15 0.018326145023226968 16-17 0.021037889632869143 18-19 0.02880135202337699 20-21 0.02265618883539754 22-23 0.013471247415641778 24-25 0.002930433691064933 26-27 0.0026024000689307985 28-29 0.0012683966722519858 30-31 0.006232638820548551 32-33 0.001202789947825159 34-35 0.0016182992025283957 36-37 0.005838998473987589 38-39 0.006998050605528198 40-41 0.0034115496701949966 42-43 6.779361524105441E-4 44-45 0.001202789947825159 46-47 0.0026024000689307985 48-49 8.310185094064735E-4 50-51 0.001771381559524325 52-53 9.184941419755759E-4 54-55 0.002864826966638106 56-57 0.002514924436361696 58-59 0.0022524975386543885 60-61 5.904605198414417E-4 62-63 0.0012683966722519858 64-65 0.0033678118539104446 66-67 0.0024930555282194206 68-69 0.0065388035345404095 70-71 0.00953484395003217 72-73 0.003914534557467335 74-75 0.002974171507349484 76-77 0.003280336221341343 78-79 0.03341569164139715 80-81 0.02289674682496257 82-83 0.0038051900167559577 84-85 0.008463267451060665 86-87 0.007063657329955024 88-89 0.0056203093925648335 90-91 0.011809210396828832 92-93 0.0141710524761946 94-95 0.007107395146239576 96-97 0.0038926656493250594 98-99 0.022218810672552027 100-101 0.041026071674909056 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2286351.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.731751599865525 #Duplication Level Percentage of deduplicated Percentage of total 1 79.4479234288227 47.455636273755744 2 12.337105655259387 14.738338609225 3 3.644328467661599 6.530463683360437 4 1.5412019337926313 3.682347642981353 5 0.8231937791072506 2.4585403166094433 6 0.48356854859635906 1.7330637855759132 7 0.3197598303823374 1.3369870332009037 8 0.23321033549666492 1.114404946432647 9 0.16208260878001507 0.8713330313675438 >10 0.8859332082574535 9.858747197456326 >50 0.0756104125631169 3.1143168430102097 >100 0.042704546130622266 4.70213465347549 >500 0.002643061421742065 1.1116570760794202 >1k 5.873469826092222E-4 0.6767561638745092 >5k 1.4683674565230554E-4 0.6152727435950858 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7784 0.3404551619589468 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6246 0.273186400513307 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3585 0.15680007138011617 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3220 0.14083576843625498 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.1868908142275618E-5 0.0 16-17 0.0 0.0 0.0 4.3737816284551236E-5 0.0 18-19 0.0 0.0 0.0 6.560672442682686E-5 0.0 20-21 0.0 0.0 0.0 8.747563256910247E-5 0.0 22-23 0.0 0.0 0.0 2.4055798956503177E-4 0.0 24-25 0.0 0.0 0.0 3.280336221341343E-4 0.0 26-27 0.0 0.0 0.0 6.341983361259929E-4 0.0 28-29 0.0 0.0 0.0 0.001487085753674742 0.0 30-31 0.0 0.0 0.0 0.002755482425926728 0.0 32-33 0.0 0.0 0.0 0.005401620311142078 0.0 34-35 0.0 0.0 0.0 0.008594480899914318 0.0 36-37 0.0 0.0 0.0 0.012246588559674346 0.0 38-39 0.0 0.0 0.0 0.0158768273112921 0.0 40-41 0.0 0.0 0.0 0.019988182042039913 0.0 42-43 0.0 0.0 0.0 0.0248430796496251 0.0 44-45 0.0 0.0 0.0 0.02985105961420622 0.0 46-47 0.0 0.0 0.0 0.038314327065266884 0.0 48-49 0.0 0.0 0.0 0.048592713892136416 0.0 50-51 4.3737816284551236E-5 0.0 0.0 0.05908978980042871 0.0 52-53 4.3737816284551236E-5 0.0 0.0 0.07619127596768825 0.0 54-55 4.3737816284551236E-5 0.0 0.0 0.10641410702031315 0.0 56-57 4.3737816284551236E-5 0.0 0.0 0.15288553682264883 0.0 58-59 4.3737816284551236E-5 0.0 0.0 0.19789174977945206 0.0 60-61 4.3737816284551236E-5 0.0 0.0 0.2428104871036862 0.0 62-63 4.3737816284551236E-5 0.0 0.0 0.2948803573904444 0.0 64-65 4.3737816284551236E-5 0.0 0.0 0.3551948060468406 0.0 66-67 4.3737816284551236E-5 0.0 0.0 0.4387996418747603 0.0 68-69 4.3737816284551236E-5 0.0 0.0 0.5425020042854312 0.0 70-71 4.3737816284551236E-5 0.0 0.0 0.6488286356731753 0.0 72-73 4.3737816284551236E-5 0.0 0.0 0.7568391729878745 0.0 74-75 4.3737816284551236E-5 0.0 0.0 0.8771837744948172 0.0 76-77 4.3737816284551236E-5 0.0 0.0 1.0090970284090237 0.0 78-79 4.3737816284551236E-5 0.0 0.0 1.1362647292563566 0.0 80-81 4.3737816284551236E-5 0.0 0.0 1.2773629245903189 0.0 82-83 4.3737816284551236E-5 0.0 0.0 1.4312763000956545 0.0 84-85 4.3737816284551236E-5 0.0 0.0 1.6208359958728997 0.0 86-87 4.3737816284551236E-5 0.0 0.0 1.8454734203103549 0.0 88-89 4.3737816284551236E-5 0.0 0.0 2.10201758172739 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13690 0.0 29.090223 1 GGTATCA 9600 0.0 27.474401 1 GTACATG 10615 0.0 24.354845 1 ATCAACG 16245 0.0 24.308031 3 TCAACGC 16390 0.0 24.122501 4 TATCAAC 16465 0.0 24.01262 2 CAACGCA 16485 0.0 23.901194 5 GAGTACT 8130 0.0 23.612665 12-13 AACGCAG 16875 0.0 23.319624 6 TACATGG 10785 0.0 23.220331 2 ACATGGG 10825 0.0 21.948788 3 GTACTTT 8850 0.0 21.528204 14-15 ACGCAGA 18495 0.0 21.094841 7 AGTACTT 8545 0.0 20.96445 12-13 CGCAGAG 18630 0.0 20.890966 8 AGAGTAC 14450 0.0 20.143072 10-11 GCAGAGT 19505 0.0 19.563972 9 ACTTTTT 10490 0.0 18.388762 16-17 TATACCG 130 0.007539243 18.280188 5 TACTTTT 9905 0.0 17.988024 14-15 >>END_MODULE