FastQCFastQC Report
Thu 26 May 2016
SRR936454_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936454_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2286351
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77780.3401927350612395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71130.3111070872320129No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42370.18531712759764357No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36640.16025535886659573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103750.027.523731
GTATCAA144650.026.1780321
GTACATG100750.022.4364591
GTAAGGT7700.022.205684
GAGTACT96350.022.17875512-13
ATCAACG175700.021.5465073
TCAACGC176000.021.4809114
CAACGCA177150.021.3391325
TATCAAC176800.021.3332522
TACATGG104800.021.2130622
AACGCAG180950.020.917256
GTACTTT103300.020.640614-15
ACATGGG103450.020.5701683
TAAGGTG9700.020.0732145
AGTACTT99800.019.77046212-13
ACGCAGA202500.018.5505287
AGAGTAC162150.018.5380110-11
CGCAGAG203850.018.4276778
ACTTTTT121150.017.99260516-17
CATGGGG45950.017.881874
Produced by FastQC (version 0.11.5)