##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936454_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2286351 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.29530855061187 31.0 31.0 34.0 30.0 34.0 2 31.505312613855004 31.0 31.0 34.0 30.0 34.0 3 31.779552658362604 31.0 31.0 34.0 30.0 34.0 4 35.62004215450734 37.0 35.0 37.0 33.0 37.0 5 35.40320536960423 37.0 35.0 37.0 33.0 37.0 6 35.51063769298765 37.0 35.0 37.0 33.0 37.0 7 35.43679295086363 37.0 35.0 37.0 33.0 37.0 8 35.41083280738609 37.0 35.0 37.0 33.0 37.0 9 37.0104734574875 39.0 37.0 39.0 33.0 39.0 10-11 36.88185716016483 39.0 37.0 39.0 33.0 39.0 12-13 36.73897052552299 39.0 36.0 39.0 32.0 39.0 14-15 37.80877804851487 39.5 37.0 41.0 32.5 41.0 16-17 37.71194755310974 39.0 37.0 41.0 32.0 41.0 18-19 37.77055578955287 39.5 37.0 41.0 32.0 41.0 20-21 37.85392487855102 40.0 37.0 41.0 32.0 41.0 22-23 37.76826852045028 39.5 37.0 41.0 32.0 41.0 24-25 37.65226555327681 39.0 37.0 41.0 32.0 41.0 26-27 37.430930333968846 39.0 36.0 41.0 32.0 41.0 28-29 37.334513379616695 39.0 36.0 41.0 31.5 41.0 30-31 37.31922023346372 39.0 36.0 41.0 31.5 41.0 32-33 37.143538109415395 39.0 36.0 41.0 31.0 41.0 34-35 36.97299496009143 39.0 35.5 41.0 31.0 41.0 36-37 36.7322591763032 39.0 35.0 40.5 30.0 41.0 38-39 36.57115464773344 39.0 35.0 40.0 30.0 41.0 40-41 36.32451819515026 39.0 35.0 40.0 30.0 41.0 42-43 36.072871357022606 38.0 35.0 40.0 30.0 41.0 44-45 35.789261797510534 38.0 35.0 40.0 28.5 41.0 46-47 35.39319946937282 38.0 34.5 40.0 27.0 41.0 48-49 34.9486275291939 38.0 34.0 40.0 26.0 41.0 50-51 34.74149529096801 38.0 34.0 40.0 25.5 41.0 52-53 34.45050344413434 38.0 33.0 40.0 24.0 41.0 54-55 34.15791144929191 38.0 33.0 40.0 24.0 41.0 56-57 33.87128704210333 37.0 33.0 40.0 23.0 41.0 58-59 33.49788921298611 37.0 32.5 40.0 22.5 41.0 60-61 33.11157954312352 36.0 32.0 39.0 22.0 40.5 62-63 32.79946320578074 36.0 31.0 39.0 21.5 40.0 64-65 32.342355570076506 35.5 31.0 39.0 20.0 40.0 66-67 31.92658716881179 35.0 31.0 38.0 18.5 40.0 68-69 31.459924132383875 35.0 30.0 38.0 18.0 40.0 70-71 30.967502583811495 34.0 29.5 37.0 15.0 39.0 72-73 30.53637521098029 34.0 29.0 36.5 12.5 39.0 74-75 30.144889389249506 34.0 29.0 36.0 9.0 39.0 76-77 28.759955273709068 32.5 27.0 34.5 8.0 37.0 78-79 29.558025430041145 34.0 29.0 35.0 7.0 37.0 80-81 29.396048113347426 34.0 29.0 35.0 6.5 37.0 82-83 29.04030067999183 34.0 29.0 35.0 2.0 36.5 84-85 28.55591464302725 33.0 28.0 35.0 2.0 36.0 86-87 28.127972039288807 33.0 27.0 35.0 2.0 36.0 88-89 27.672396320599944 33.0 26.0 35.0 2.0 35.0 90-91 27.160095059769915 32.5 26.0 34.5 2.0 35.0 92-93 26.63483428397477 32.0 25.0 34.0 2.0 35.0 94-95 26.023122652646073 32.0 24.5 34.0 2.0 35.0 96-97 25.250959717033822 32.0 21.5 34.0 2.0 35.0 98-99 24.262278845199184 31.0 10.0 34.0 2.0 35.0 100-101 21.333533215153754 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4340289359295326 1101 2 0.4425917989011694 1101 3 0.27359126663772315 1101 4 0.14229610913147894 1101 5 0.2639236705192687 1101 6 0.29015373971817837 1101 7 0.32844922896317286 1101 8 0.2731290664167503 1101 9 0.41033883984975006 1101 10-11 0.33838350419121355 1101 12-13 0.3575963256834882 1101 14-15 0.49507060839360406 1101 16-17 0.3935058369413156 1101 18-19 0.32943975901837064 1101 20-21 0.24381525513852864 1101 22-23 0.21544336806507403 1101 24-25 0.2546075544331714 1101 26-27 0.18169981171394056 1101 28-29 0.20520146080424695 1101 30-31 0.12119047719883724 1101 32-33 0.13170648949490982 1101 34-35 0.3083843141259379 1101 36-37 0.3338792800909971 1101 38-39 0.2969465026507834 1101 40-41 0.3094049001633934 1101 42-43 0.3062962444984052 1101 44-45 0.42991603227945774 1101 46-47 0.2788617515641434 1101 48-49 0.307292579321377 1101 50-51 0.38146613277483965 1101 52-53 0.270308123419305 1101 54-55 0.15631647189755427 1101 56-57 0.218879415981462 1101 58-59 0.22683660357866842 1101 60-61 0.2536817768499233 1101 62-63 0.11706866714995812 1101 64-65 0.24478232590597315 1101 66-67 0.22420960579372107 1101 68-69 0.011085572988250192 1101 70-71 0.07149946719193778 1101 72-73 0.14436765426719944 1101 74-75 0.2036157437841446 1101 76-77 0.5128592295377459 1101 78-79 0.4331767194222138 1101 80-81 0.2892546392826638 1101 82-83 0.4797793443635072 1101 84-85 0.6299293591190782 1101 86-87 0.4058984436994564 1101 88-89 0.24558435046151672 1101 90-91 0.303537814778263 1101 92-93 0.5392166027975698 1101 94-95 0.24953350295385235 1101 96-97 0.4125158394762529 1101 98-99 0.3377616689558387 1101 100-101 0.272758505905486 1102 1 0.4887251041071856 1102 2 0.6145754437070075 1102 3 0.31972815353670114 1102 4 0.21240454874487824 1102 5 0.27490886026233596 1102 6 0.28530997298340566 1102 7 0.2781304412803891 1102 8 0.13976027722893036 1102 9 0.12430617146244316 1102 10-11 0.2491343282860754 1102 12-13 0.3173039671376472 1102 14-15 0.34704302842892076 1102 16-17 0.42738866601749237 1102 18-19 0.3880637218558718 1102 20-21 0.39422026739931937 1102 22-23 0.18039177332163803 1102 24-25 0.18832024440899886 1102 26-27 -0.01084044100614534 1102 28-29 0.2252216807915488 1102 30-31 0.37838175050852385 1102 32-33 0.43625171653488337 1102 34-35 0.5988776650156993 1102 36-37 0.572750586889498 1102 38-39 0.5350114775166759 1102 40-41 0.5328052216111345 1102 42-43 0.5493490145701685 1102 44-45 0.445546533974543 1102 46-47 0.6333213315400243 1102 48-49 0.6721740979659714 1102 50-51 0.4678409519079807 1102 52-53 0.38713122635608244 1102 54-55 0.5098813428109423 1102 56-57 0.611199320891167 1102 58-59 0.5936857821438721 1102 60-61 0.5061331484279776 1102 62-63 0.5029498168856676 1102 64-65 0.4160888793939108 1102 66-67 0.422898631699244 1102 68-69 0.5698355388624314 1102 70-71 0.547388173609761 1102 72-73 0.5152762306638934 1102 74-75 0.037905300162453415 1102 76-77 0.3238451367981092 1102 78-79 0.44807176589283415 1102 80-81 0.5808577794183378 1102 82-83 0.6288851253128271 1102 84-85 0.7250426638497238 1102 86-87 0.5796323931876408 1102 88-89 0.47383771386757445 1102 90-91 0.4697559456352174 1102 92-93 0.5300042057788055 1102 94-95 0.7210144656450552 1102 96-97 0.649871609750317 1102 98-99 0.446859648217135 1102 100-101 0.09886766513622192 1103 1 0.5245989409046103 1103 2 0.508628652712293 1103 3 0.3848957203923433 1103 4 0.219103090349698 1103 5 0.17300817386089307 1103 6 0.19651406246371295 1103 7 0.2614714434812626 1103 8 0.27650601302779165 1103 9 0.16781510926297472 1103 10-11 0.22800445630828392 1103 12-13 0.3567720081191368 1103 14-15 0.40009050871456253 1103 16-17 0.347017926249805 1103 18-19 0.3832235156884849 1103 20-21 0.2948094010591973 1103 22-23 0.2509909647055011 1103 24-25 0.21887492780236784 1103 26-27 0.2514098819178514 1103 28-29 0.400101679393849 1103 30-31 0.3985626316269659 1103 32-33 0.25843377729818684 1103 34-35 0.2530237504745969 1103 36-37 0.210241370389042 1103 38-39 0.26283572487990625 1103 40-41 0.33324868878595026 1103 42-43 0.3879449717582375 1103 44-45 0.35990645064344307 1103 46-47 0.33173912573180786 1103 48-49 0.4482953275584336 1103 50-51 0.1999619489985136 1103 52-53 0.20868801822604155 1103 54-55 0.3310902217636169 1103 56-57 0.3893516664306915 1103 58-59 0.4727839434803869 1103 60-61 0.3317044622603831 1103 62-63 0.19440557289572524 1103 64-65 0.11127768556877982 1103 66-67 0.17024900856570824 1103 68-69 0.17765385272288725 1103 70-71 0.11565134226487217 1103 72-73 0.16668991457786575 1103 74-75 0.23586607016715178 1103 76-77 0.25109673058701887 1103 78-79 0.40647601315190585 1103 80-81 0.4147233136796977 1103 82-83 0.5909432981669198 1103 84-85 0.6169590894888408 1103 86-87 0.5171454861200324 1103 88-89 0.456494397735014 1103 90-91 0.3259166333570853 1103 92-93 0.6723329275440584 1103 94-95 0.6180238424945479 1103 96-97 0.3350410782835205 1103 98-99 0.410534330992288 1103 100-101 0.47344976225314994 1104 1 0.3016744178138353 1104 2 0.4575987962927712 1104 3 0.13107755359429873 1104 4 0.060201918571571866 1104 5 -0.004333067159485893 1104 6 0.05411488489988159 1104 7 0.09678587828832974 1104 8 0.12639403772710267 1104 9 0.016469873093683418 1104 10-11 0.031665359398132864 1104 12-13 0.036881775548494034 1104 14-15 0.27085193256373685 1104 16-17 0.08521823082786284 1104 18-19 0.07107755162647322 1104 20-21 -0.06170190981482193 1104 22-23 0.0049455048478606045 1104 24-25 0.19810634926991355 1104 26-27 0.14867453094745287 1104 28-29 0.0452853646179463 1104 30-31 0.11845555232603289 1104 32-33 0.18706314814992453 1104 34-35 0.1529592353924798 1104 36-37 0.19520047231812043 1104 38-39 0.1622421894025905 1104 40-41 0.3357929039909564 1104 42-43 0.33057973755258985 1104 44-45 0.6380693082813025 1104 46-47 0.857432060494375 1104 48-49 0.7434628102523746 1104 50-51 0.6075557314886026 1104 52-53 0.4479695568976396 1104 54-55 0.5961604187952361 1104 56-57 0.6378319313883551 1104 58-59 0.5849392176457187 1104 60-61 0.37256204734742937 1104 62-63 0.1636079806064643 1104 64-65 0.2801365701364489 1104 66-67 0.4526231308057227 1104 68-69 0.5440979620254645 1104 70-71 0.39132850066398817 1104 72-73 0.47102162044613394 1104 74-75 0.34492958687182096 1104 76-77 0.5787630302585889 1104 78-79 0.6097672002376093 1104 80-81 0.6591963003657604 1104 82-83 0.6667791708801047 1104 84-85 0.7720091075271647 1104 86-87 0.8398571881167385 1104 88-89 0.9114425947138507 1104 90-91 0.7562398529350496 1104 92-93 0.9018189426378989 1104 94-95 0.8410944915906882 1104 96-97 0.5870162380099799 1104 98-99 0.852126299651335 1104 100-101 0.8365539539468649 1105 1 0.37326130406409774 1105 2 0.3930402000794757 1105 3 0.14153972118123903 1105 4 0.10293192892783765 1105 5 -0.10492598901069528 1105 6 -0.0057676906829087216 1105 7 0.10654534504317326 1105 8 -0.030118073207987095 1105 9 0.1154553627861219 1105 10-11 0.08804997162776118 1105 12-13 0.05497455961523201 1105 14-15 0.1512045390615242 1105 16-17 0.14435901323862055 1105 18-19 0.06780380847725098 1105 20-21 0.15802267503734413 1105 22-23 0.16717286781959473 1105 24-25 0.06357911344441192 1105 26-27 0.20379400931464176 1105 28-29 0.29485334619712233 1105 30-31 0.13992133242969373 1105 32-33 0.1210134355106689 1105 34-35 0.08666287251815419 1105 36-37 0.18250219084302444 1105 38-39 0.12771508174869695 1105 40-41 0.016584322004703722 1105 42-43 0.1675496998957655 1105 44-45 0.022842873912857442 1105 46-47 0.30230008539555087 1105 48-49 0.08836547353018176 1105 50-51 0.04086468017381861 1105 52-53 -0.011309762998187978 1105 54-55 0.1796614166111965 1105 56-57 0.08824938631040169 1105 58-59 0.27916285979572564 1105 60-61 0.26069763432212056 1105 62-63 0.13096363374332753 1105 64-65 0.20383753488412992 1105 66-67 0.20439510326420773 1105 68-69 0.0498297161564345 1105 70-71 0.09322777957052963 1105 72-73 -0.030603194986753124 1105 74-75 0.055222103316236115 1105 76-77 0.0540118113466157 1105 78-79 0.10272232799907144 1105 80-81 -0.052131281907595195 1105 82-83 0.08132900335981219 1105 84-85 0.24964220092325107 1105 86-87 -0.01918480602838102 1105 88-89 0.10162402482700372 1105 90-91 0.03850016562250147 1105 92-93 0.2947112182478655 1105 94-95 0.43291671052317326 1105 96-97 0.18369215546233875 1105 98-99 0.13332655304659724 1105 100-101 0.45827131889244654 1106 1 0.31443010535982907 1106 2 0.41167442824187717 1106 3 0.150134687039003 1106 4 0.025370976037535797 1106 5 -0.1297063583653113 1106 6 -0.06436443082171195 1106 7 -0.05294081683208418 1106 8 0.03867824820049037 1106 9 -0.01931823261697474 1106 10-11 -0.15709927075424446 1106 12-13 -0.004654986346253054 1106 14-15 0.03431981536777329 1106 16-17 0.043647811695507244 1106 18-19 0.03158114824699254 1106 20-21 -0.11390363653654845 1106 22-23 0.020843237453028962 1106 24-25 -0.03959160597585054 1106 26-27 -0.03773847206807801 1106 28-29 0.08654110885408528 1106 30-31 0.12927826010999866 1106 32-33 -0.04661905258897292 1106 34-35 -0.22684379434284097 1106 36-37 -0.10122530747140956 1106 38-39 -0.15937063947120578 1106 40-41 -0.029250980991804454 1106 42-43 -0.06850199568322779 1106 44-45 -0.026834935661163684 1106 46-47 -0.046180829043542815 1106 48-49 -0.10416390032603573 1106 50-51 0.14327887467557332 1106 52-53 0.017971339514701867 1106 54-55 0.016929983828561035 1106 56-57 -0.1300600533716434 1106 58-59 -0.2394098229932453 1106 60-61 -0.06853819234076042 1106 62-63 -0.4816015077175635 1106 64-65 -0.2346829143384781 1106 66-67 -0.18752520110102822 1106 68-69 -0.12921702284175396 1106 70-71 -0.2231190835005954 1106 72-73 0.01385260450428305 1106 74-75 -0.051122994978928915 1106 76-77 -0.09115606098626117 1106 78-79 -0.2307034950138629 1106 80-81 -0.28065091686194776 1106 82-83 0.06647233041557143 1106 84-85 0.06684277832499319 1106 86-87 -0.013922795413112254 1106 88-89 0.05422276039829654 1106 90-91 0.029527214536894775 1106 92-93 0.3217872221107321 1106 94-95 0.20376728501002717 1106 96-97 0.12773362864788496 1106 98-99 0.11366712312309701 1106 100-101 0.234540975391635 1107 1 0.6283958583996991 1107 2 0.6394022799228019 1107 3 0.42053584863138127 1107 4 0.2892302323142175 1107 5 0.07032366412988722 1107 6 0.1969140108409846 1107 7 0.22885986089593757 1107 8 0.20137686949597366 1107 9 0.35668913319087636 1107 10-11 0.4460124651330659 1107 12-13 0.4680751224410358 1107 14-15 0.5827988291559052 1107 16-17 0.5099868502061327 1107 18-19 0.4727927853418663 1107 20-21 0.5378151874414954 1107 22-23 0.6054102117328597 1107 24-25 0.454888554158579 1107 26-27 0.5125714261968497 1107 28-29 0.5901025318625486 1107 30-31 0.7225517579962357 1107 32-33 0.717978843777054 1107 34-35 0.6991087560247706 1107 36-37 0.7822895788631001 1107 38-39 0.8641389432504383 1107 40-41 0.856375324928031 1107 42-43 0.7566346726037878 1107 44-45 0.6977865270991117 1107 46-47 0.7782225280566948 1107 48-49 0.9280433546045046 1107 50-51 0.7776927649388412 1107 52-53 0.5040588393724477 1107 54-55 0.5334836326004151 1107 56-57 0.6271583064499495 1107 58-59 0.5389743959404711 1107 60-61 0.5048785500595514 1107 62-63 0.38227863063158907 1107 64-65 0.2731624044193097 1107 66-67 0.4580599656909108 1107 68-69 0.5420265388955698 1107 70-71 0.4860423296810126 1107 72-73 0.5916618077538658 1107 74-75 0.8223719287446016 1107 76-77 0.7941855550874415 1107 78-79 0.8526169676649396 1107 80-81 0.914129323711812 1107 82-83 0.8256405266579172 1107 84-85 1.0409323895120046 1107 86-87 1.2293463941908662 1107 88-89 1.3299366572188198 1107 90-91 1.1619291279561352 1107 92-93 1.206759337498994 1107 94-95 1.4961032031320727 1107 96-97 1.6036865493290655 1107 98-99 1.696462372776761 1107 100-101 1.695163636884029 1108 1 0.3941436415521764 1108 2 0.3870638823897501 1108 3 0.19064272623051792 1108 4 0.13994320089382484 1108 5 0.08608311262695878 1108 6 0.03150864155649913 1108 7 0.01237468394850083 1108 8 0.05376676736516828 1108 9 0.18912005850904734 1108 10-11 0.04497548800694062 1108 12-13 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-3.873121964797246 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 22.0 4 46.0 5 89.0 6 207.0 7 521.0 8 1267.0 9 2220.0 10 3642.0 11 5500.0 12 7672.0 13 10275.0 14 13131.0 15 16882.0 16 20420.0 17 24941.0 18 21991.0 19 16456.0 20 15614.0 21 16570.0 22 17927.0 23 20678.0 24 24179.0 25 29040.0 26 36393.0 27 44038.0 28 54334.0 29 65835.0 30 80347.0 31 97839.0 32 118430.0 33 146997.0 34 185914.0 35 241353.0 36 324685.0 37 393591.0 38 216981.0 39 10319.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.190027061375055 19.779903172950643 15.259805108607852 22.77026465706645 2 15.898677078681075 20.957168109385645 39.038886266735545 24.105268545197735 3 19.6893503353321 24.458751286500416 30.032791818535316 25.819106559632164 4 13.405715955438364 17.34746458147791 36.64537560168182 32.601443861401904 5 13.750075994455793 37.58246218537749 33.9135154663479 14.753946353818815 6 31.897989416323213 36.44108013161584 17.081891625564054 14.579038826496893 7 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29.627953013338722 21.88471936286248 30-31 22.087589845734435 26.17316381419376 29.59645933397322 22.142787006098587 32-33 21.88805008696504 26.388559323979937 29.4831719965787 22.240218592476328 34-35 22.554242224969453 26.380289208764307 29.18006780701833 21.885400759247908 36-37 22.571321660365673 26.11498287938986 29.21849668861572 22.095198771628755 38-39 22.186413045261055 26.34197120079078 29.393628921237912 22.077986832710252 40-41 22.484413449703577 26.314763898863912 29.211495950165027 21.98932670126748 42-43 22.27862087789873 26.427508954635314 28.89452000595773 22.399350161508224 44-45 22.493876569129164 26.486747146043825 28.64694047150418 22.372435813322834 46-47 22.660094536156556 26.82632928622009 28.195843890687268 22.31773228693609 48-49 22.852551821154684 26.911722507711715 27.900095007637354 22.335630663496246 50-51 22.651136751314326 27.08394202087179 27.87684683817805 22.388074389635836 52-53 23.004723994792474 27.05293450375092 27.652132380326083 22.290209121130523 54-55 22.58954668290374 26.944206049885267 27.79140344845385 22.674843818757147 56-57 22.785526565851868 27.15499887043162 27.564158499985673 22.49531606373084 58-59 23.084042351948057 26.812658346883143 27.60288105217232 22.500418248996475 60-61 22.813684886660948 26.866605532155653 28.058553535307585 22.261156045875815 62-63 22.673341272002194 26.92726495362002 28.029143443873284 22.370250330504497 64-65 22.513821791897414 26.969888321102587 27.89936103808727 22.61692884891273 66-67 22.537270705528346 27.144253360098798 27.58827168769173 22.730204246681126 68-69 22.6281248018125 26.903092053036616 27.778528612261056 22.69025453288983 70-71 22.610107827970367 26.916709639522256 27.7202254970527 22.75295703545468 72-73 22.667341978550102 26.867593820896268 27.767258832961346 22.69780536759229 74-75 22.53691581038957 27.27512092412757 27.50247009317467 22.68549317230819 76-77 22.662724106281175 27.026526993132887 27.57679715299378 22.733951747592158 78-79 22.697539936661244 27.000135600930836 27.543851591342538 22.758472871065386 80-81 22.58738809990361 27.32425978998029 27.38289873223088 22.705453377885224 82-83 22.607397440518476 27.291031666322557 27.417382882898643 22.68418801026032 84-85 22.5778965205623 27.243901820246403 27.294160461977086 22.88404119721421 86-87 22.63248417311093 27.219580944482423 27.25151248326328 22.89642239914336 88-89 22.5736176832939 27.246899018605887 27.019772256366466 23.15971104173375 90-91 22.464100823711842 27.2762649063582 27.218889221617793 23.040745048312164 92-93 22.630232252985174 27.169990814853694 27.30713379696453 22.892643135196604 94-95 22.397566237893248 27.084614131260874 27.32173534586738 23.196084284978497 96-97 22.44492230114245 26.914235998414792 27.40733920233897 23.233502498103785 98-99 22.32297926087123 27.022587224084287 27.058433711883033 23.595999803161448 100-101 22.135057669237643 27.115488293915725 27.21261888436517 23.536835152481466 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 394.0 1 303.5 2 277.5 3 334.0 4 280.5 5 251.5 6 443.5 7 938.0 8 1489.0 9 1840.5 10 1782.5 11 1563.5 12 1643.0 13 1907.5 14 2178.0 15 2394.0 16 2643.5 17 2928.5 18 3223.0 19 3547.5 20 3939.5 21 4566.5 22 5372.0 23 6350.5 24 7675.0 25 9360.0 26 11766.5 27 14753.0 28 18000.5 29 21869.5 30 26340.5 31 31198.5 32 36636.5 33 40800.0 34 44841.5 35 48777.5 36 53089.0 37 58277.0 38 61527.5 39 63305.5 40 65347.0 41 69593.5 42 73548.0 43 76637.0 44 79667.5 45 83155.0 46 84760.0 47 87905.0 48 97032.0 49 96819.5 50 88571.5 51 83039.0 52 78171.5 53 76615.0 54 75200.0 55 69529.0 56 62726.5 57 56561.5 58 51239.0 59 45402.5 60 38494.0 61 31955.5 62 26698.0 63 21044.5 64 15576.0 65 12141.0 66 9351.0 67 7465.5 68 5616.0 69 3787.0 70 2901.5 71 2294.0 72 1898.5 73 1713.0 74 1437.5 75 1181.5 76 864.5 77 546.5 78 372.0 79 282.5 80 199.5 81 143.5 82 101.0 83 63.5 84 37.0 85 22.0 86 13.5 87 6.5 88 4.0 89 4.0 90 1.5 91 1.0 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0211467967954178 2 0.011984161661967039 3 0.004811159791300636 4 0.01132809441769877 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0034115496701949966 10-11 3.0616471399185863E-4 12-13 1.5308235699592931E-4 14-15 2.1868908142275618E-5 16-17 0.0 18-19 4.3737816284551236E-5 20-21 0.0 22-23 0.0013558723048210882 24-25 0.0 26-27 0.0 28-29 0.0 30-31 6.560672442682684E-5 32-33 0.003827058924898233 34-35 4.1550925470323674E-4 36-37 0.0031272538643454134 38-39 1.5308235699592931E-4 40-41 0.003717714384186855 42-43 0.010693896081572778 44-45 0.002230628630512113 46-47 0.0013121344885365371 48-49 0.00957858176631672 50-51 4.811159791300636E-4 52-53 0.0019463328246625297 54-55 0.009841008664024027 56-57 0.003477156394621823 58-59 0.0014652168455324664 60-61 0.0059264741065566924 62-63 0.003214729496914516 64-65 0.016511025647418093 66-67 0.0031272538643454134 68-69 2.624268977073074E-4 70-71 0.0010497075908292295 72-73 0.0 74-75 0.0 76-77 8.528874175487491E-4 78-79 0.010103435561731336 80-81 0.01432413483319053 82-83 0.011503045682836974 84-85 0.007741593482365569 86-87 0.00918494141975576 88-89 0.027248659545275417 90-91 0.02359655188551539 92-93 0.002230628630512113 94-95 0.007807200206792395 96-97 0.009491106133747618 98-99 0.009338023776751688 100-101 0.011087536428133739 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2286351.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.50167209773082 #Duplication Level Percentage of deduplicated Percentage of total 1 78.49725065445871 45.922204183605295 2 12.897137762701794 15.09008248785684 3 3.8621354352339257 6.778241424872461 4 1.5831165259626503 3.7045985557746284 5 0.8341684234803051 2.4400123792362933 6 0.5126892268321698 1.799590622170484 7 0.3325330895748926 1.3617619237569 8 0.2408889450924175 1.1273924862211915 9 0.1734801109849554 0.913398891148783 >10 0.9359927008750556 10.118544575276223 >50 0.0819871627857216 3.291435869331052 >100 0.04455966881476603 4.721052285018688 >500 0.0030828152094201436 1.2153380132258904 >1k 8.270967635028172E-4 0.8613244647643008 >5k 1.5038122972778497E-4 0.6550218377409327 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7778 0.3401927350612395 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7113 0.3111070872320129 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4237 0.18531712759764357 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3664 0.16025535886659573 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.3737816284551236E-5 0.0 5 0.0 0.0 0.0 4.3737816284551236E-5 0.0 6 0.0 0.0 0.0 4.3737816284551236E-5 0.0 7 0.0 0.0 0.0 4.3737816284551236E-5 0.0 8 0.0 0.0 0.0 4.3737816284551236E-5 0.0 9 0.0 0.0 0.0 4.3737816284551236E-5 0.0 10-11 0.0 0.0 0.0 4.3737816284551236E-5 0.0 12-13 0.0 0.0 0.0 4.3737816284551236E-5 0.0 14-15 0.0 0.0 0.0 4.3737816284551236E-5 0.0 16-17 0.0 0.0 0.0 4.3737816284551236E-5 0.0 18-19 0.0 0.0 0.0 4.3737816284551236E-5 0.0 20-21 0.0 0.0 0.0 4.3737816284551236E-5 0.0 22-23 0.0 0.0 0.0 2.1868908142275617E-4 0.0 24-25 0.0 0.0 0.0 3.280336221341343E-4 0.0 26-27 0.0 0.0 0.0 6.341983361259929E-4 0.0 28-29 0.0 0.0 0.0 0.0014652168455324664 0.0 30-31 0.0 4.3737816284551236E-5 0.0 0.0027773513340690033 0.0 32-33 0.0 6.560672442682686E-5 0.0 0.005445358127426629 0.0 34-35 0.0 8.747563256910247E-5 0.0 0.008769432165052523 0.0 36-37 0.0 8.747563256910247E-5 0.0 0.012487146549239377 0.0 38-39 0.0 8.747563256910247E-5 0.0 0.016139254208999407 0.0 40-41 0.0 8.747563256910247E-5 0.0 0.02016313330717812 0.0 42-43 0.0 8.747563256910247E-5 0.0 0.024996162006621032 0.0 44-45 0.0 8.747563256910247E-5 0.0 0.029960404154917596 0.0 46-47 0.0 8.747563256910247E-5 0.0 0.038467409422262816 0.0 48-49 0.0 8.747563256910247E-5 0.0 0.04865832061656325 0.0 50-51 0.0 8.747563256910247E-5 0.0 0.05915539652485555 0.0 52-53 0.0 8.747563256910247E-5 0.0 0.07643183395725328 0.0 54-55 0.0 8.747563256910247E-5 0.0 0.10632663138774405 0.0 56-57 0.0 8.747563256910247E-5 0.0 0.15306048808778705 4.3737816284551236E-5 58-59 0.0 1.3121344885365371E-4 0.0 0.19797922541202118 4.3737816284551236E-5 60-61 0.0 1.3121344885365371E-4 0.0 0.24291983164439757 4.3737816284551236E-5 62-63 0.0 1.3121344885365371E-4 0.0 0.29468353721716395 4.3737816284551236E-5 64-65 0.0 1.7495126513820494E-4 0.0 0.3546480833432837 4.3737816284551236E-5 66-67 0.0 1.7495126513820494E-4 0.0 0.43761872083507736 4.3737816284551236E-5 68-69 0.0 1.7495126513820494E-4 0.0 0.540577540368911 4.3737816284551236E-5 70-71 0.0 1.7495126513820494E-4 0.0 0.6470135162973665 4.3737816284551236E-5 72-73 0.0 1.7495126513820494E-4 0.0 0.755680120856334 4.3737816284551236E-5 74-75 0.0 1.7495126513820494E-4 0.0 0.8758279021899962 4.3737816284551236E-5 76-77 0.0 1.7495126513820494E-4 0.0 1.0076536804716336 4.3737816284551236E-5 78-79 0.0 1.7495126513820494E-4 0.0 1.1340997073502712 4.3737816284551236E-5 80-81 0.0 1.7495126513820494E-4 0.0 1.274366884174827 4.3737816284551236E-5 82-83 0.0 1.7495126513820494E-4 0.0 1.4275148478951833 4.3737816284551236E-5 84-85 0.0 1.7495126513820494E-4 0.0 1.6150188663070542 4.3737816284551236E-5 86-87 0.0 1.7495126513820494E-4 0.0 1.837797433552416 4.3737816284551236E-5 88-89 0.0 1.7495126513820494E-4 0.0 2.0914767680028135 4.3737816284551236E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10375 0.0 27.52373 1 GTATCAA 14465 0.0 26.178032 1 GTACATG 10075 0.0 22.436459 1 GTAAGGT 770 0.0 22.20568 4 GAGTACT 9635 0.0 22.178755 12-13 ATCAACG 17570 0.0 21.546507 3 TCAACGC 17600 0.0 21.480911 4 CAACGCA 17715 0.0 21.339132 5 TATCAAC 17680 0.0 21.333252 2 TACATGG 10480 0.0 21.213062 2 AACGCAG 18095 0.0 20.91725 6 GTACTTT 10330 0.0 20.6406 14-15 ACATGGG 10345 0.0 20.570168 3 TAAGGTG 970 0.0 20.073214 5 AGTACTT 9980 0.0 19.770462 12-13 ACGCAGA 20250 0.0 18.550528 7 AGAGTAC 16215 0.0 18.53801 10-11 CGCAGAG 20385 0.0 18.427677 8 ACTTTTT 12115 0.0 17.992605 16-17 CATGGGG 4595 0.0 17.88187 4 >>END_MODULE