FastQCFastQC Report
Thu 26 May 2016
SRR936453_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936453_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2280773
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73970.324319868746254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61140.2680670106143838No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35890.1573589305029479No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32440.14223247995306854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA135800.029.2896581
GGTATCA93550.026.7704371
GTACATG108000.024.5966931
TCAACGC160450.024.4942384
ATCAACG161250.024.4016513
CAACGCA162300.024.1543565
TATCAAC164500.023.948442
AACGCAG165600.023.67256
TACATGG109850.023.576812
ACATGGG108800.023.0181473
GAGTACT80550.022.65449112-13
ACGCAGA182050.021.4817287
CGCAGAG182900.021.3549778
GTACTTT88550.020.9293214-15
CCACGCG2056.813061E-720.8607379
AGTACTT85000.020.54598812-13
GCAGAGT191850.020.135849
AGAGTAC145050.019.96714810-11
CATGGGG47800.018.4919724
CATGGGA59600.018.4189074
Produced by FastQC (version 0.11.5)