##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936453_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2280773 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.333455367982697 31.0 31.0 34.0 30.0 34.0 2 31.36898455041339 31.0 31.0 34.0 28.0 34.0 3 31.42074112592529 31.0 31.0 34.0 28.0 34.0 4 35.0574581512496 37.0 35.0 37.0 32.0 37.0 5 35.028614421514106 37.0 35.0 37.0 32.0 37.0 6 35.1649629314272 37.0 35.0 37.0 32.0 37.0 7 35.02951411648595 37.0 35.0 37.0 32.0 37.0 8 34.97314287743673 37.0 35.0 37.0 32.0 37.0 9 36.44368553994632 39.0 37.0 39.0 32.0 39.0 10-11 36.35279705608581 39.0 36.0 39.0 32.0 39.0 12-13 36.23482389523201 39.0 35.0 39.0 32.0 39.0 14-15 37.27457291891828 39.5 37.0 41.0 32.0 41.0 16-17 37.14443546113533 39.0 36.0 41.0 31.0 41.0 18-19 37.0873859432745 39.0 36.0 41.0 31.0 41.0 20-21 36.99634575646064 39.0 36.0 41.0 31.0 41.0 22-23 36.86165918309275 39.0 36.0 41.0 30.5 41.0 24-25 37.008945870544764 39.0 36.0 41.0 31.0 41.0 26-27 36.99096030161704 39.0 36.0 41.0 31.0 41.0 28-29 36.88674409947855 39.0 36.0 41.0 30.0 41.0 30-31 36.6995595352979 39.0 36.0 41.0 30.0 41.0 32-33 36.52457543999337 39.0 35.0 41.0 30.0 41.0 34-35 36.305924789534075 39.0 35.0 40.0 30.0 41.0 36-37 36.11575351865355 38.5 35.0 40.0 29.5 41.0 38-39 35.90189992603385 38.0 35.0 40.0 29.0 41.0 40-41 35.71285678145085 38.0 35.0 40.0 28.0 41.0 42-43 35.47293549160745 38.0 34.5 40.0 27.0 41.0 44-45 35.209506382266014 38.0 34.0 40.0 26.5 41.0 46-47 34.91529341148812 38.0 34.0 40.0 25.0 41.0 48-49 34.80021904854188 38.0 34.0 40.0 24.0 41.0 50-51 33.88433197867565 37.5 33.0 39.5 23.5 40.5 52-53 34.03523016100243 37.5 33.0 39.5 24.0 40.5 54-55 34.45216051750876 38.0 34.0 40.0 24.0 41.0 56-57 34.37004099048875 38.0 34.0 40.0 23.5 41.0 58-59 34.109936631133394 38.0 33.5 40.0 23.0 41.0 60-61 33.790722706731444 37.0 33.0 40.0 22.5 41.0 62-63 33.459142580169086 37.0 33.0 40.0 22.0 41.0 64-65 33.11884150680493 36.0 32.0 39.0 21.0 41.0 66-67 32.73623767029862 36.0 32.0 39.0 20.0 41.0 68-69 32.33152444368642 35.0 31.0 39.0 18.5 40.0 70-71 31.906740390209812 35.0 31.0 38.0 17.5 40.0 72-73 31.512060604014515 35.0 31.0 37.5 15.0 39.5 74-75 31.080857893354576 35.0 30.5 37.0 12.5 39.0 76-77 30.648185067080327 34.5 30.0 36.5 9.0 39.0 78-79 30.20176755863034 34.0 30.0 36.0 7.0 38.5 80-81 29.72414725183085 34.0 29.0 35.5 4.5 37.0 82-83 29.242265889678627 34.0 29.0 35.0 2.0 37.0 84-85 28.917341839806063 34.0 29.0 35.0 2.0 36.0 86-87 28.591919932408878 34.0 29.0 35.0 2.0 36.0 88-89 28.185773639025015 34.0 27.5 35.0 2.0 36.0 90-91 27.76912739672032 33.5 27.0 35.0 2.0 35.0 92-93 27.38509794705567 33.0 26.0 35.0 2.0 35.0 94-95 26.906870828442813 33.0 25.0 35.0 2.0 35.0 96-97 26.301700563800082 33.0 24.5 35.0 2.0 35.0 98-99 25.560194942679523 32.5 22.0 35.0 2.0 35.0 100-101 23.018519598399315 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.38595941272754075 1101 2 0.5800443622617415 1101 3 0.5664715161198153 1101 4 0.5860539240374578 1101 5 0.5361403512882106 1101 6 0.36306033081785927 1101 7 0.4595717442304519 1101 8 0.5841793532493966 1101 9 0.6855458298924901 1101 10-11 0.6967208408826266 1101 12-13 0.9287186208354186 1101 14-15 1.1800775635661438 1101 16-17 1.1300619977809419 1101 18-19 1.1389253434931206 1101 20-21 1.0387747664563136 1101 22-23 0.9068304553643358 1101 24-25 1.002051330066088 1101 26-27 0.9160227636541549 1101 28-29 0.9028787968839325 1101 30-31 0.96824256335276 1101 32-33 0.8790140587400899 1101 34-35 0.7332275452305197 1101 36-37 0.7982063409108662 1101 38-39 0.7703205779988096 1101 40-41 0.630766500008562 1101 42-43 0.7488837268494777 1101 44-45 0.8068341512377089 1101 46-47 0.8402362292554955 1101 48-49 1.145073513401492 1101 50-51 1.1416408672905618 1101 52-53 1.150904818525163 1101 54-55 1.1605856303329958 1101 56-57 0.8836244012327654 1101 58-59 0.9278876777351641 1101 60-61 0.9245624080822807 1101 62-63 0.9287221313982172 1101 64-65 0.8973806312940269 1101 66-67 0.93026660079979 1101 68-69 0.9289885563407765 1101 70-71 0.897219151953994 1101 72-73 0.8552268998348502 1101 74-75 0.7926249305120834 1101 76-77 0.9108064862356002 1101 78-79 0.8028918233688387 1101 80-81 0.9330243592476748 1101 82-83 1.0751272258834241 1101 84-85 0.9964764283847387 1101 86-87 0.8564665825821862 1101 88-89 0.8354185753633274 1101 90-91 0.7196537481137142 1101 92-93 0.7805166326917075 1101 94-95 0.8784753614474923 1101 96-97 0.8953508312363745 1101 98-99 1.0634328868137537 1101 100-101 1.456744218029307 1102 1 0.2894418543084747 1102 2 0.33563836850482076 1102 3 0.2764358589586635 1102 4 0.36897365358838385 1102 5 0.35983740452692103 1102 6 0.217339770496487 1102 7 0.22301767113214765 1102 8 0.25361791103910036 1102 9 0.421116875439175 1102 10-11 0.4316186048046049 1102 12-13 0.4518504743754903 1102 14-15 0.34932599487106586 1102 16-17 0.41571383192550826 1102 18-19 0.42363763231630003 1102 20-21 0.43385344417382044 1102 22-23 0.2141798357772018 1102 24-25 0.08455392470793299 1102 26-27 0.2037039706515742 1102 28-29 0.387595832372881 1102 30-31 0.1581657557176257 1102 32-33 0.1723098437528563 1102 34-35 0.1161377814499005 1102 36-37 0.1698467972629345 1102 38-39 0.12751237430517648 1102 40-41 0.12078169800082605 1102 42-43 -0.004066438530237804 1102 44-45 -0.1530268130318504 1102 46-47 0.10122809530666643 1102 48-49 0.2136657787962335 1102 50-51 0.28141588226247904 1102 52-53 0.20434517242547656 1102 54-55 0.33120349203628763 1102 56-57 0.4435628366312443 1102 58-59 0.410926662932674 1102 60-61 0.27316932231124724 1102 62-63 0.4420800977325712 1102 64-65 0.38277224767751505 1102 66-67 0.4016872553442141 1102 68-69 0.18686797821074919 1102 70-71 0.13915653303832798 1102 72-73 0.2304486635399421 1102 74-75 0.08499935998104746 1102 76-77 0.28709893489669014 1102 78-79 0.11329890539417065 1102 80-81 0.3419740416454964 1102 82-83 0.32917501441357544 1102 84-85 0.23617014371263778 1102 86-87 0.2568934880003688 1102 88-89 0.15950679543815482 1102 90-91 0.28173493931200966 1102 92-93 0.01118518670659796 1102 94-95 0.2635116361948988 1102 96-97 0.2548234524162396 1102 98-99 0.3374177976136856 1102 100-101 0.3620812373622684 1103 1 0.2988081861104419 1103 2 0.3764330488581429 1103 3 0.30300215769496575 1103 4 0.4174579305156527 1103 5 0.35876909083089714 1103 6 0.29198018555374006 1103 7 0.30634031800199324 1103 8 0.159638074108706 1103 9 0.49711551089837513 1103 10-11 0.4984186485151483 1103 12-13 0.4461417406324273 1103 14-15 0.3730271571092132 1103 16-17 0.5479590254702558 1103 18-19 0.47316386022422563 1103 20-21 0.7091980348024265 1103 22-23 0.5436206351812203 1103 24-25 0.6264298090439766 1103 26-27 0.4959228613584088 1103 28-29 0.1707315617693368 1103 30-31 0.2864323414831844 1103 32-33 0.41992726137436165 1103 34-35 0.20493256292959927 1103 36-37 0.49744511730098395 1103 38-39 0.4386001498392176 1103 40-41 0.5004134482905229 1103 42-43 0.5949868489169958 1103 44-45 0.6359706702852037 1103 46-47 0.4598037701880173 1103 48-49 0.5543625428685601 1103 50-51 0.5890170431533761 1103 52-53 0.578723839816 1103 54-55 0.5981720575336666 1103 56-57 0.5524722036117211 1103 58-59 0.4630684547557067 1103 60-61 0.5097643805246079 1103 62-63 0.4713291562010795 1103 64-65 0.5497829602542268 1103 66-67 0.3355442004394078 1103 68-69 0.483679386996009 1103 70-71 0.6391440585673287 1103 72-73 0.5827189923121132 1103 74-75 0.42332821996908265 1103 76-77 0.5335780986214615 1103 78-79 0.5784425722133584 1103 80-81 0.4494088429912111 1103 82-83 0.3783896300360361 1103 84-85 0.4528857186219213 1103 86-87 0.6606613437502418 1103 88-89 0.5905860916584373 1103 90-91 0.6332005815418533 1103 92-93 0.561993041764552 1103 94-95 0.43712508626932234 1103 96-97 0.5005405091263491 1103 98-99 0.6878095542171287 1103 100-101 1.2015006156046937 1104 1 0.08923079307981041 1104 2 0.22323985249947853 1104 3 0.29762038252901846 1104 4 0.31369478718416133 1104 5 0.31270045123707746 1104 6 0.21580230002333423 1104 7 0.3079419670856538 1104 8 0.3089750148125532 1104 9 0.3777545893791796 1104 10-11 0.49594809748345625 1104 12-13 0.20588925397706248 1104 14-15 0.40739380298664685 1104 16-17 0.32762247419844925 1104 18-19 0.47980177333695195 1104 20-21 0.5717907445174433 1104 22-23 0.19935099594755457 1104 24-25 0.4045538299737501 1104 26-27 -0.0028707777230039255 1104 28-29 0.33230193916705275 1104 30-31 0.2591171972615669 1104 32-33 -0.0036781766195659316 1104 34-35 -0.13329136342382242 1104 36-37 -0.0990381356570964 1104 38-39 0.10918090135521652 1104 40-41 0.2297559815898964 1104 42-43 0.1704166149942452 1104 44-45 0.2253175078334806 1104 46-47 0.29691649758851213 1104 48-49 0.18269284565929667 1104 50-51 0.1224503697521726 1104 52-53 0.17343634819756915 1104 54-55 0.30479650156817684 1104 56-57 0.244720602694656 1104 58-59 0.3708060009824621 1104 60-61 0.37028255938811583 1104 62-63 0.5422028601683095 1104 64-65 0.4101100831712685 1104 66-67 0.43802851335537696 1104 68-69 0.3539187266598063 1104 70-71 0.47841769460355366 1104 72-73 0.3729966602876189 1104 74-75 0.04933071467661421 1104 76-77 0.22396791131343008 1104 78-79 0.21000911385184295 1104 80-81 0.29973590734704914 1104 82-83 0.3425889665375301 1104 84-85 0.37519329380719313 1104 86-87 0.3743263092137674 1104 88-89 0.4022686137726126 1104 90-91 0.52185522559218 1104 92-93 0.4892395229678712 1104 94-95 0.4473147367467405 1104 96-97 0.37182882645783266 1104 98-99 0.5144837459992324 1104 100-101 0.844392895187621 1105 1 0.2208020185520354 1105 2 0.260343529783551 1105 3 0.31373915350658876 1105 4 0.3611490383858609 1105 5 0.3462837499873501 1105 6 0.21752242452881632 1105 7 0.23984541568619733 1105 8 0.4016182862381754 1105 9 0.35759596839375263 1105 10-11 0.3872090298428432 1105 12-13 0.27601975707761284 1105 14-15 0.04720740591631767 1105 16-17 0.15624009643789094 1105 18-19 0.23710879581094702 1105 20-21 0.2161323103934194 1105 22-23 0.18077739174096763 1105 24-25 0.24754099731934787 1105 26-27 0.24773051615113673 1105 28-29 0.1118904506894225 1105 30-31 0.2470254587927272 1105 32-33 0.18415622709586188 1105 34-35 0.05370941916914518 1105 36-37 0.24919695114236617 1105 38-39 0.02605877461127193 1105 40-41 -0.008255545453096147 1105 42-43 0.3475659000035094 1105 44-45 0.03358626193065106 1105 46-47 0.2730979566165317 1105 48-49 0.4279837448479711 1105 50-51 0.44482183925669005 1105 52-53 0.45731606169179884 1105 54-55 0.4481109746367622 1105 56-57 0.6439214517773877 1105 58-59 0.4005836310768487 1105 60-61 0.1849906154523424 1105 62-63 0.4194370147567028 1105 64-65 0.6323103786025328 1105 66-67 0.7666557285658584 1105 68-69 0.6845888416368027 1105 70-71 0.8109604542643218 1105 72-73 0.7648134206751926 1105 74-75 0.7613111101907784 1105 76-77 0.6281710514233687 1105 78-79 0.6762458864345824 1105 80-81 0.49239573672436876 1105 82-83 0.5305646851961647 1105 84-85 0.6892578952926058 1105 86-87 0.6425669002826631 1105 88-89 0.40110334993688923 1105 90-91 0.35925146430915333 1105 92-93 0.36712374241764323 1105 94-95 0.41983208390169224 1105 96-97 0.5477745563603946 1105 98-99 0.5502536778552845 1105 100-101 1.0305971387310677 1106 1 0.06665903641884796 1106 2 0.15040305322426661 1106 3 0.2986016508692373 1106 4 0.21397763285588667 1106 5 0.2273446453401604 1106 6 0.31162313729046787 1106 7 0.3033952406834288 1106 8 0.2656822440170643 1106 9 0.5596242916771601 1106 10-11 0.5209859491746087 1106 12-13 0.5969106325088376 1106 14-15 0.6597266174141652 1106 16-17 0.707266535828694 1106 18-19 0.7086020798984904 1106 20-21 0.5618883996189084 1106 22-23 0.6131197384313296 1106 24-25 0.3617772186031871 1106 26-27 0.1909314965448914 1106 28-29 0.4852894925358342 1106 30-31 0.5288034692625629 1106 32-33 0.23806133395466844 1106 34-35 0.24776902674423695 1106 36-37 0.3369868519518491 1106 38-39 0.45252255786024875 1106 40-41 0.24476677004886227 1106 42-43 0.15782103260809066 1106 44-45 0.4287558888990475 1106 46-47 0.40379687848994195 1106 48-49 0.42597308000052436 1106 50-51 0.24147867176698412 1106 52-53 0.42511746086604063 1106 54-55 0.473318595776405 1106 56-57 0.3712685155365705 1106 58-59 0.4472255444099673 1106 60-61 0.5294336540338165 1106 62-63 0.5997944586760866 1106 64-65 0.39345630837380696 1106 66-67 0.5258843402540876 1106 68-69 0.5410382307331858 1106 70-71 0.6342726387807893 1106 72-73 0.7303177557152374 1106 74-75 0.5355611321211562 1106 76-77 0.46695670540346157 1106 78-79 0.6548779560456701 1106 80-81 0.8001032771537098 1106 82-83 0.8919417236204659 1106 84-85 0.8346824526821948 1106 86-87 0.9499232286063055 1106 88-89 0.9369267401413666 1106 90-91 0.9933913354270274 1106 92-93 1.1498085616738507 1106 94-95 1.170942625058565 1106 96-97 1.113195474449057 1106 98-99 1.3738623708277444 1106 100-101 1.687576068939645 1107 1 0.1263616607227398 1107 2 0.2904493599332767 1107 3 0.18326671443011122 1107 4 0.23156049461821482 1107 5 0.2886881825146972 1107 6 0.2678569996658453 1107 7 0.3026257113462094 1107 8 0.2702441553030255 1107 9 0.29628685418736467 1107 10-11 0.4127915702410192 1107 12-13 0.38069313106616676 1107 14-15 0.2947603803130221 1107 16-17 0.3829206839613235 1107 18-19 0.49529224264671967 1107 20-21 0.5229033043900557 1107 22-23 0.6029352827311598 1107 24-25 0.4056129191094442 1107 26-27 0.28201260333050016 1107 28-29 0.4129873113780249 1107 30-31 0.39500673682228893 1107 32-33 0.29430204016090755 1107 34-35 0.4208136832224909 1107 36-37 0.48348852981497714 1107 38-39 0.36515720139625785 1107 40-41 0.19654121794770418 1107 42-43 0.41806396611175956 1107 44-45 0.48429871714266426 1107 46-47 0.42699955356027175 1107 48-49 0.42417231709010395 1107 50-51 0.3072723055262898 1107 52-53 0.3329834323108827 1107 54-55 0.3712862350596069 1107 56-57 0.43776918449693625 1107 58-59 0.47105203626869496 1107 60-61 0.369042533920414 1107 62-63 0.5545276585172303 1107 64-65 0.6895618794449021 1107 66-67 0.5682241978252947 1107 68-69 0.5284152184965407 1107 70-71 0.5844222391396308 1107 72-73 0.5006770279172024 1107 74-75 0.541692786863063 1107 76-77 0.3674189692270353 1107 78-79 0.38486890805145535 1107 80-81 0.45873652878948334 1107 82-83 0.6795129091004917 1107 84-85 0.6357703647418589 1107 86-87 0.5944124025013018 1107 88-89 0.4981064109434641 1107 90-91 0.49908488860263134 1107 92-93 0.43911078652935487 1107 94-95 0.5612345706729229 1107 96-97 0.6105726485702512 1107 98-99 0.9525595174964998 1107 100-101 1.3734002139748824 1108 1 -0.00404250915042681 1108 2 0.13002576813515887 1108 3 0.08782874873875457 1108 4 0.11357502832834854 1108 5 0.1875021036575717 1108 6 0.09266253972822369 1108 7 -0.08373597033832425 1108 8 0.13382567122727096 1108 9 0.1527220195529253 1108 10-11 -0.17177940803735936 1108 12-13 0.1102763619733409 1108 14-15 0.15158730567339518 1108 16-17 0.2379905059740821 1108 18-19 0.06778693627644117 1108 20-21 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82-83 -2.5831613970270375 2316 84-85 -2.581923542843967 2316 86-87 -3.1698744747373837 2316 88-89 -3.315324179462678 2316 90-91 -3.3715402989520413 2316 92-93 -3.8768609701316272 2316 94-95 -4.132625527909003 2316 96-97 -5.090718605476141 2316 98-99 -6.320223187249347 2316 100-101 -6.897898813614013 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4935.0 3 1988.0 4 2015.0 5 2263.0 6 2469.0 7 2894.0 8 3650.0 9 4668.0 10 5947.0 11 7585.0 12 9429.0 13 11510.0 14 13318.0 15 15616.0 16 18692.0 17 22761.0 18 19575.0 19 14823.0 20 15017.0 21 16395.0 22 18018.0 23 20316.0 24 23733.0 25 27731.0 26 32060.0 27 37868.0 28 45532.0 29 55251.0 30 66880.0 31 82314.0 32 102482.0 33 129919.0 34 169893.0 35 226987.0 36 311644.0 37 395857.0 38 297940.0 39 40787.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.30725019835709 18.55117963805807 14.353947996889445 22.787622166695396 2 15.662240052836937 19.966029638329232 40.33394979350704 24.03778051532679 3 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22.68819099531386 26.844444502904285 27.968846458736706 22.49851804304515 48-49 22.783841090729883 26.94425249961855 27.747892358121106 22.524014051530465 50-51 22.611864878670097 27.151503990985482 27.69331781222528 22.54331331811914 52-53 22.903419579162446 27.11552205692137 27.54412041969755 22.436937944218634 54-55 22.508593099474748 26.963258498059073 27.69841301161225 22.829735390853934 56-57 22.787576420607095 27.262272519672624 27.352279073377595 22.597871986342685 58-59 22.938895138653045 26.917552736084872 27.465122698424775 22.678429426837308 60-61 22.701571856074086 26.939222866008 27.932110235360742 22.42709504255717 62-63 22.603780195071625 27.015352424824595 27.901920045145033 22.47894733495875 64-65 22.381736889584563 27.12173178166608 27.73126210527354 22.76526922347582 66-67 22.444992545821275 27.280145575725683 27.417982109971064 22.856879768481978 68-69 22.51816155827617 27.051381225645354 27.64709700109544 22.783360214983034 70-71 22.51849253502291 27.052682349330237 27.538377217021466 22.89044789862539 72-73 22.608807554092472 26.959684731690466 27.672046244964317 22.759461469252745 74-75 22.445551077139946 27.423447045140897 27.381069565493814 22.749932312225344 76-77 22.60965667593092 27.114904021017004 27.439134677508996 22.83630462554308 78-79 22.603448642517122 27.155248107271806 27.402503643171546 22.838799607039526 80-81 22.574244618354545 27.49708678050604 27.19674289453981 22.731925706599608 82-83 22.682783523010038 27.409734569538745 27.230486246155127 22.676995661296086 84-85 22.557291601573162 27.388671197941918 27.158708869402314 22.895328331082606 86-87 22.62601952015385 27.3178675462918 27.200489680986077 22.85562325256827 88-89 22.541100144544124 27.32418859182279 27.009405562569977 23.125305701063112 90-91 22.566627679713594 27.307806561865938 27.191437632019433 22.93412812640104 92-93 22.72123905447504 27.229843745614772 27.24631027055093 22.802606929359264 94-95 22.444871589396527 27.18954236902035 27.28395080675803 23.081635234825093 96-97 22.501209147159894 27.09533253848991 27.336657702387996 23.066800611962204 98-99 22.368706396174296 27.178242495433093 27.008503191410284 23.444547916982327 100-101 22.38935687322185 27.076997708045177 27.204040998600178 23.3296044201328 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 313.0 1 230.5 2 237.5 3 334.0 4 299.0 5 296.5 6 467.5 7 870.5 8 1428.5 9 1744.5 10 1665.0 11 1565.0 12 1669.5 13 1865.5 14 2063.0 15 2264.5 16 2471.0 17 2614.5 18 2779.0 19 3087.5 20 3540.0 21 4102.0 22 4834.0 23 5864.5 24 7191.0 25 8927.5 26 11230.5 27 14168.5 28 17342.5 29 21206.5 30 25961.5 31 30861.5 32 36149.0 33 40354.5 34 44541.5 35 48607.0 36 52962.5 37 57927.0 38 61206.0 39 63064.5 40 65200.5 41 69449.0 42 73610.5 43 76832.0 44 79789.0 45 83520.5 46 85203.0 47 87789.0 48 96291.0 49 96538.0 50 88477.0 51 82971.0 52 78354.5 53 76298.0 54 74698.0 55 69765.0 56 63466.5 57 56914.5 58 51714.5 59 45944.0 60 38873.0 61 32676.5 62 27387.0 63 21537.0 64 15940.0 65 12343.0 66 9479.5 67 7639.5 68 5824.0 69 3972.5 70 2967.5 71 2304.5 72 1914.0 73 1727.0 74 1454.5 75 1191.0 76 866.5 77 546.5 78 355.5 79 256.0 80 174.5 81 107.5 82 83.0 83 56.0 84 25.5 85 21.0 86 20.0 87 13.5 88 7.5 89 5.0 90 7.5 91 7.0 92 4.0 93 3.0 94 2.5 95 2.0 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0348127586568238 2 0.024114631311401877 3 0.022141616022287182 4 0.050640725753943946 5 0.05520058331100903 6 0.04748390129136043 7 0.050640725753943946 8 0.04450245596558711 9 0.04419554247616926 10-11 0.052197215593134436 12-13 0.04581779949166357 14-15 0.04018374472163604 16-17 0.04910615830685473 18-19 0.05344679194290708 20-21 0.011881936518890746 22-23 0.021133185985628557 24-25 0.006182114572559391 26-27 0.010676204953320651 28-29 0.006730174375091252 30-31 0.009273171858839087 32-33 0.003814496225621752 34-35 0.008374353782686835 36-37 0.009426628603548008 38-39 0.011553100637371628 40-41 0.0018195585444057783 42-43 0.0019730152891146995 44-45 0.002258006386431267 46-47 2.192239210127444E-4 48-49 0.0010961196050637218 50-51 3.50758273620391E-4 52-53 0.0036391170888115565 54-55 0.007190544609218015 56-57 0.016814474741677493 58-59 0.010632360169118102 60-61 0.011070808011143591 62-63 0.005414830849014786 64-65 2.1922392101274437E-5 66-67 0.007585147667040955 68-69 0.01784482717043739 70-71 0.0053929084569135114 72-73 0.002148394425924895 74-75 0.0035295051283051842 76-77 0.006291726533065763 78-79 0.0059848130436479215 80-81 0.009667774916662026 82-83 0.006050580219951744 84-85 0.017515991288918273 86-87 0.004581779949166357 88-89 0.0067740191592938 90-91 0.004187176891343417 92-93 0.01705562105479151 94-95 0.011246187147953786 96-97 0.01052274820861173 98-99 0.022426607119603748 100-101 0.03689538590644487 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2280773.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.60564247083521 #Duplication Level Percentage of deduplicated Percentage of total 1 79.33265339865456 47.28673774742894 2 12.435513555723265 14.824535498873315 3 3.6400262218596358 6.5089830469389165 4 1.5558574571901784 3.709515333154423 5 0.8161664715754573 2.43240634507049 6 0.49322697000965965 1.7639466258821481 7 0.3011381152466801 1.2564671582214348 8 0.22904195127064594 1.0921754126608467 9 0.16306199412437486 0.8747473430123028 >10 0.9090854023830145 9.984588100105046 >50 0.07863520504851516 3.233440160199055 >100 0.04211784364113196 4.634404474607138 >500 0.002883853566792856 1.226176805102176 >1k 4.4366977950653554E-4 0.5763736189906405 >5k 1.478899265021785E-4 0.5955023297531961 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7397 0.324319868746254 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6114 0.2680670106143838 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3589 0.1573589305029479 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3244 0.14223247995306854 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.1922392101274437E-5 0.0 20-21 0.0 0.0 0.0 4.3844784202548875E-5 0.0 22-23 0.0 0.0 0.0 8.768956840509775E-5 0.0 24-25 0.0 0.0 0.0 2.849910973165677E-4 0.0 26-27 0.0 0.0 0.0 4.1652544992421426E-4 0.0 28-29 0.0 0.0 0.0 8.330508998484286E-4 0.0 30-31 0.0 0.0 0.0 0.00232377356273509 0.0 32-33 0.0 0.0 0.0 0.005480598025318609 0.0 34-35 0.0 0.0 0.0 0.009514318171953105 0.0 36-37 0.0 0.0 0.0 0.014688002707853871 0.0 38-39 0.0 0.0 0.0 0.01931362744122278 0.0 40-41 0.0 0.0 0.0 0.02352272672466747 0.0 42-43 0.0 0.0 0.0 0.029814453257733235 0.0 44-45 0.0 0.0 0.0 0.036259636535507914 0.0 46-47 0.0 0.0 0.0 0.046365859294195434 0.0 48-49 0.0 0.0 0.0 0.05844509734199765 0.0 50-51 0.0 0.0 0.0 0.0712696967212432 0.0 52-53 0.0 0.0 0.0 0.09053947937826343 0.0 54-55 0.0 4.3844784202548875E-5 0.0 0.12149389702526292 0.0 56-57 0.0 4.3844784202548875E-5 0.0 0.1690216431008259 4.3844784202548875E-5 58-59 0.0 4.3844784202548875E-5 0.0 0.21497097694509712 4.3844784202548875E-5 60-61 0.0 4.3844784202548875E-5 0.0 0.26701473579352264 4.3844784202548875E-5 62-63 0.0 4.3844784202548875E-5 0.0 0.3240568000410387 4.3844784202548875E-5 64-65 0.0 4.3844784202548875E-5 0.0 0.3857244890219237 4.3844784202548875E-5 66-67 0.0 4.3844784202548875E-5 0.0 0.4693364924961844 4.3844784202548875E-5 68-69 0.0 4.3844784202548875E-5 0.0 0.5758793181083781 6.576717630382332E-5 70-71 0.0 4.3844784202548875E-5 0.0 0.6826194452494834 8.768956840509775E-5 72-73 0.0 4.3844784202548875E-5 0.0 0.7947744032396034 8.768956840509775E-5 74-75 0.0 4.3844784202548875E-5 0.0 0.9139883714863338 8.768956840509775E-5 76-77 0.0 4.3844784202548875E-5 0.0 1.0487891605170703 8.768956840509775E-5 78-79 0.0 4.3844784202548875E-5 0.0 1.1803015907326158 8.768956840509775E-5 80-81 0.0 4.3844784202548875E-5 0.0 1.326984316282243 8.768956840509775E-5 82-83 0.0 4.3844784202548875E-5 0.0 1.484759772235115 8.768956840509775E-5 84-85 0.0 4.3844784202548875E-5 0.0 1.6842096955725099 8.768956840509775E-5 86-87 0.0 4.3844784202548875E-5 0.0 1.9147017261253092 8.768956840509775E-5 88-89 0.0 4.3844784202548875E-5 0.0 2.169352232773713 8.768956840509775E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13580 0.0 29.289658 1 GGTATCA 9355 0.0 26.770437 1 GTACATG 10800 0.0 24.596693 1 TCAACGC 16045 0.0 24.494238 4 ATCAACG 16125 0.0 24.401651 3 CAACGCA 16230 0.0 24.154356 5 TATCAAC 16450 0.0 23.94844 2 AACGCAG 16560 0.0 23.6725 6 TACATGG 10985 0.0 23.57681 2 ACATGGG 10880 0.0 23.018147 3 GAGTACT 8055 0.0 22.654491 12-13 ACGCAGA 18205 0.0 21.481728 7 CGCAGAG 18290 0.0 21.354977 8 GTACTTT 8855 0.0 20.92932 14-15 CCACGCG 205 6.813061E-7 20.860737 9 AGTACTT 8500 0.0 20.545988 12-13 GCAGAGT 19185 0.0 20.13584 9 AGAGTAC 14505 0.0 19.967148 10-11 CATGGGG 4780 0.0 18.491972 4 CATGGGA 5960 0.0 18.418907 4 >>END_MODULE