FastQCFastQC Report
Thu 26 May 2016
SRR936453_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936453_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2280773
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79810.34992522272054255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71670.31423556837966776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41870.1835781114560721No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36420.159682704065683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101150.028.2333371
GTATCAA141550.027.7954731
GTACATG102400.024.4548071
TACATGG104800.023.3883292
ACATGGG103350.022.8871383
ATCAACG170600.022.8857653
TCAACGC171650.022.7724464
CAACGCA174150.022.4187555
TATCAAC175100.022.3538252
AACGCAG178250.021.9563836
GAGTACT95400.021.80903612-13
CCCGCGA4200.020.92183920-21
CGCGGAA3900.020.7048182-83
AGTACTT99400.020.5968912-13
GTAAGGT8800.020.5094764
GTACTTT101250.020.40821314-15
TCGCGCT4250.020.11888160-61
ATTCGCG3900.020.09562580-81
TAAGGTG9500.019.9985965
AGAGTAC155900.019.91222210-11
Produced by FastQC (version 0.11.5)