##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936453_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2280773 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.648207866368114 31.0 31.0 34.0 30.0 34.0 2 31.791970090842007 31.0 31.0 34.0 30.0 34.0 3 31.893386145837397 31.0 31.0 34.0 30.0 34.0 4 35.68387384452552 37.0 35.0 37.0 33.0 37.0 5 35.43136997851167 37.0 35.0 37.0 33.0 37.0 6 35.53173419713404 37.0 35.0 37.0 33.0 37.0 7 35.47147787175664 37.0 35.0 37.0 33.0 37.0 8 35.44508111942749 37.0 35.0 37.0 33.0 37.0 9 37.03606891172423 39.0 37.0 39.0 33.0 39.0 10-11 36.8852871372995 39.0 37.0 39.0 33.0 39.0 12-13 36.77973103855579 39.0 37.0 39.0 32.0 39.0 14-15 37.8154868985208 39.5 37.0 41.0 33.0 41.0 16-17 37.730455639381915 39.0 37.0 41.0 32.0 41.0 18-19 37.88917419664298 39.5 37.0 41.0 33.0 41.0 20-21 37.928498145146406 40.0 37.0 41.0 33.0 41.0 22-23 37.833402973465574 40.0 37.0 41.0 32.0 41.0 24-25 37.69910815324454 39.0 37.0 41.0 32.0 41.0 26-27 37.463339841360806 39.0 36.0 41.0 32.0 41.0 28-29 37.36206408967486 39.0 36.0 41.0 31.5 41.0 30-31 37.32433631054033 39.0 36.0 41.0 31.5 41.0 32-33 37.17104529911569 39.0 36.0 41.0 31.0 41.0 34-35 36.98822351018711 39.0 36.0 41.0 31.0 41.0 36-37 36.71100521621398 39.0 35.0 40.5 30.0 41.0 38-39 36.5440341936703 39.0 35.0 40.0 30.0 41.0 40-41 36.30951282744929 39.0 35.0 40.0 30.0 41.0 42-43 36.04479731213935 38.0 35.0 40.0 29.5 41.0 44-45 35.789694108094054 38.0 35.0 40.0 29.0 41.0 46-47 35.42854900509607 38.0 34.5 40.0 27.5 41.0 48-49 35.06285390961749 38.0 34.0 40.0 26.5 41.0 50-51 34.72214727199945 38.0 34.0 40.0 25.5 41.0 52-53 34.426828535763974 38.0 33.0 40.0 24.0 41.0 54-55 34.14246683032463 38.0 33.0 40.0 24.0 41.0 56-57 33.80857060303678 37.0 33.0 40.0 23.0 41.0 58-59 33.45500735934703 37.0 32.0 39.5 22.5 41.0 60-61 33.074333570241315 36.0 32.0 39.0 22.0 40.0 62-63 32.65301895453866 36.0 31.0 39.0 20.5 40.0 64-65 32.21631723104403 35.0 31.0 39.0 19.5 40.0 66-67 31.73350592101888 35.0 30.5 38.0 18.0 40.0 68-69 31.427004572572542 35.0 30.0 38.0 17.5 40.0 70-71 30.973000820335912 34.0 29.5 37.0 14.0 39.0 72-73 30.507009904098304 34.0 29.0 36.5 11.5 39.0 74-75 29.954254982850113 34.0 29.0 36.0 8.0 39.0 76-77 28.586546315657017 32.0 27.0 34.5 7.0 36.5 78-79 29.370975980511872 33.5 29.0 35.0 7.0 37.0 80-81 29.157730734272988 34.0 29.0 35.0 4.0 37.0 82-83 28.79309207886975 33.5 28.5 35.0 2.0 36.5 84-85 28.340287262257135 33.0 27.0 35.0 2.0 36.0 86-87 27.909419525748508 33.0 26.5 35.0 2.0 35.5 88-89 27.45157606653534 33.0 26.0 35.0 2.0 35.0 90-91 26.896398940183875 32.0 25.5 34.0 2.0 35.0 92-93 26.321302470697432 32.0 25.0 34.0 2.0 35.0 94-95 25.625821377226053 32.0 23.5 34.0 2.0 35.0 96-97 24.739254410675677 31.0 19.0 34.0 2.0 35.0 98-99 23.63350539488147 31.0 2.0 34.0 2.0 35.0 100-101 20.305279832758455 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38045650346497695 1101 2 0.4376990675158865 1101 3 0.3943555415050177 1101 4 0.25512470193270786 1101 5 0.1606873171762615 1101 6 0.3053273881136249 1101 7 0.2955244204387881 1101 8 0.36126635633890913 1101 9 0.46835532655914847 1101 10-11 0.2717167843934547 1101 12-13 0.4265018502415927 1101 14-15 0.6964922376967451 1101 16-17 0.6387402526097574 1101 18-19 0.5426505663476959 1101 20-21 0.46302721772870825 1101 22-23 0.4137071516679214 1101 24-25 0.3986905186643881 1101 26-27 0.4869045911283152 1101 28-29 0.37784422140488516 1101 30-31 0.5589911803252647 1101 32-33 0.5576941876459571 1101 34-35 0.5699494883251219 1101 36-37 0.48972413298917417 1101 38-39 0.5406332505587486 1101 40-41 0.6102512189975968 1101 42-43 0.6639183321326882 1101 44-45 0.7155040584262835 1101 46-47 0.8031096213578834 1101 48-49 0.7761978303691066 1101 50-51 0.7290953988792168 1101 52-53 0.7977109452717812 1101 54-55 0.8585170700829821 1101 56-57 0.7507261425962355 1101 58-59 0.5907960088300328 1101 60-61 0.559755880774766 1101 62-63 0.6408177279575185 1101 64-65 0.677632931196797 1101 66-67 0.5314443645563038 1101 68-69 0.4568493263122555 1101 70-71 0.4839312294018505 1101 72-73 0.6329161297463806 1101 74-75 0.5943272339598273 1101 76-77 0.41760979622618777 1101 78-79 0.2860352755307112 1101 80-81 0.5898211842430072 1101 82-83 0.6078442844146466 1101 84-85 0.6736478372640491 1101 86-87 0.6966798760486768 1101 88-89 0.4218758607310029 1101 90-91 0.45085398090176554 1101 92-93 0.47587350885888213 1101 94-95 0.8347630509563828 1101 96-97 1.1671678111417805 1101 98-99 1.5472704277621112 1101 100-101 1.787613872152047 1102 1 0.25850114741567154 1102 2 0.3049921670245368 1102 3 0.3011675785643497 1102 4 0.17795067270814258 1102 5 0.04739826411356063 1102 6 0.2533493190953706 1102 7 0.25005690590905516 1102 8 0.20641764591618283 1102 9 0.27518699125467805 1102 10-11 0.3940504011985908 1102 12-13 0.3314069922838314 1102 14-15 0.4476374574318598 1102 16-17 0.3345650750039084 1102 18-19 0.22638542314130916 1102 20-21 0.11931863736809589 1102 22-23 0.13401411542504604 1102 24-25 0.336914482639628 1102 26-27 0.09700081851647013 1102 28-29 0.08406432845432477 1102 30-31 0.1936416152428464 1102 32-33 0.317565043689811 1102 34-35 0.2994023588712409 1102 36-37 0.36681875084186544 1102 38-39 0.34261041008812043 1102 40-41 0.4680932565437317 1102 42-43 0.5479297428400187 1102 44-45 0.4696682615329948 1102 46-47 0.2978750364581373 1102 48-49 0.3633894032010616 1102 50-51 0.378240141522447 1102 52-53 0.23697285260335832 1102 54-55 0.39864060119347755 1102 56-57 0.5517667018158292 1102 58-59 0.2268628210957715 1102 60-61 0.26665507285414236 1102 62-63 0.23627057806179863 1102 64-65 0.4905657071491518 1102 66-67 0.3352770546355792 1102 68-69 0.36573569096047365 1102 70-71 0.3391673647635933 1102 72-73 0.20848010380048265 1102 74-75 0.04499794303027471 1102 76-77 -0.03182461429422645 1102 78-79 0.08996601717298702 1102 80-81 0.3431593115932756 1102 82-83 0.22890331751882798 1102 84-85 0.29901412308296926 1102 86-87 0.39274428379904336 1102 88-89 0.23042741220642782 1102 90-91 0.2584507422898241 1102 92-93 0.3218674182284005 1102 94-95 0.4357324275130132 1102 96-97 0.7905017790962461 1102 98-99 0.7610969800907235 1102 100-101 0.9287447184981481 1103 1 0.15550725774421537 1103 2 0.18943848097637073 1103 3 0.2104420816271677 1103 4 0.1801774489439083 1103 5 0.02107047726268263 1103 6 0.18749775099615107 1103 7 0.24713122664500276 1103 8 0.18341804792679994 1103 9 0.1860407623454705 1103 10-11 0.17296061145330555 1103 12-13 0.22070155096948696 1103 14-15 0.20239434745641915 1103 16-17 0.35531519322030647 1103 18-19 0.28269626633404954 1103 20-21 0.3155433135682415 1103 22-23 0.2656465629545153 1103 24-25 0.2598882834229883 1103 26-27 0.3729293807139342 1103 28-29 0.4351980665925552 1103 30-31 0.358867147857012 1103 32-33 0.4788544648893307 1103 34-35 0.5009683540620813 1103 36-37 0.45588090181656327 1103 38-39 0.6245767825734987 1103 40-41 0.6353542070085965 1103 42-43 0.5044816593429005 1103 44-45 0.6158541753936149 1103 46-47 0.6108931116339562 1103 48-49 0.5786872960633218 1103 50-51 0.6250041390016037 1103 52-53 0.4527255646780972 1103 54-55 0.4950373252975879 1103 56-57 0.4840684130304922 1103 58-59 0.6774206711223556 1103 60-61 0.6023598862867843 1103 62-63 0.7437525994383876 1103 64-65 0.5562689122066686 1103 66-67 0.7739652791840435 1103 68-69 0.7678550037867744 1103 70-71 0.8216201917198909 1103 72-73 0.5842735425281269 1103 74-75 0.6493580367651113 1103 76-77 0.44639088435445373 1103 78-79 0.395224306084728 1103 80-81 0.43659941974527783 1103 82-83 0.5687106518092016 1103 84-85 0.48632861730387944 1103 86-87 0.34618972437158035 1103 88-89 0.3188655069788098 1103 90-91 0.15651660051097238 1103 92-93 0.4122908666412215 1103 94-95 0.7127510921491691 1103 96-97 0.5544667414674187 1103 98-99 0.8851773828797498 1103 100-101 1.1845503788808394 1104 1 0.16425337440400156 1104 2 0.2566169726152907 1104 3 0.1506803671891923 1104 4 0.05408638040680103 1104 5 0.005119867786895327 1104 6 0.1346901802029663 1104 7 0.11235260265774372 1104 8 -0.03171387583821428 1104 9 0.06868117016573905 1104 10-11 0.09331224223659262 1104 12-13 0.0786020437620607 1104 14-15 0.0904860718790843 1104 16-17 0.08242947249286203 1104 18-19 0.055804519658131824 1104 20-21 -0.06675963528146411 1104 22-23 0.13234148540482948 1104 24-25 0.11033832631907359 1104 26-27 0.018867575213285193 1104 28-29 0.15982203042515408 1104 30-31 0.08144146935219965 1104 32-33 0.1035700770150143 1104 34-35 0.05074611427149733 1104 36-37 0.12852593597239093 1104 38-39 0.27890240632358143 1104 40-41 0.32362117585110894 1104 42-43 0.15818726628591406 1104 44-45 0.2893058902122334 1104 46-47 0.10721546766854573 1104 48-49 0.22067480495730507 1104 50-51 0.006395729026287711 1104 52-53 0.17355329809576148 1104 54-55 0.2218448902346566 1104 56-57 0.2586648530945652 1104 58-59 0.0297659011654261 1104 60-61 0.28026298505319147 1104 62-63 0.21013480502922732 1104 64-65 0.43855941679932897 1104 66-67 0.09835192371457069 1104 68-69 0.2405515718070852 1104 70-71 0.12239129644783375 1104 72-73 -0.06538694885898266 1104 74-75 0.2619324461021115 1104 76-77 0.18095122143278175 1104 78-79 0.08841041653702675 1104 80-81 0.3428407270657239 1104 82-83 0.32971723116110496 1104 84-85 0.3033204450870741 1104 86-87 0.28258639458119816 1104 88-89 0.42921166228899565 1104 90-91 0.58229685542792 1104 92-93 0.42515969245968677 1104 94-95 0.451216729895382 1104 96-97 0.6636394765394371 1104 98-99 0.8355416336037145 1104 100-101 1.3103713338384857 1105 1 0.14878789281589633 1105 2 0.13519414314994194 1105 3 0.16437042361520682 1105 4 0.005182542605723484 1105 5 0.029649336587809216 1105 6 0.06700346632275966 1105 7 0.06772416664092873 1105 8 0.14517035296268688 1105 9 0.013507559961524862 1105 10-11 0.01236570458974029 1105 12-13 -0.049880003194161304 1105 14-15 0.11698016502731434 1105 16-17 0.1410780759981165 1105 18-19 0.13838153992976743 1105 20-21 0.22492448414344324 1105 22-23 0.11862361742410599 1105 24-25 0.1786608365239033 1105 26-27 0.19230567023948453 1105 28-29 0.16427969865926428 1105 30-31 0.1510313803197576 1105 32-33 0.14468160086571658 1105 34-35 0.2280802453530839 1105 36-37 0.11423394076661708 1105 38-39 0.23193368843787 1105 40-41 0.18688006450460648 1105 42-43 0.18370779379291236 1105 44-45 0.3030979223388499 1105 46-47 0.20847338831871554 1105 48-49 0.27975009414104335 1105 50-51 0.14195827535872496 1105 52-53 0.11173449980643824 1105 54-55 0.21409425330974585 1105 56-57 0.17251255669851417 1105 58-59 0.21890236932046747 1105 60-61 0.11471700312150546 1105 62-63 0.24289689709014795 1105 64-65 0.14485569482329907 1105 66-67 0.333075480085288 1105 68-69 0.4095125708174763 1105 70-71 0.2803279563978087 1105 72-73 0.22265450180460888 1105 74-75 0.040286688887793076 1105 76-77 -0.1272576370552585 1105 78-79 0.14654640069040425 1105 80-81 0.3743668575127188 1105 82-83 0.3508481279765263 1105 84-85 0.1475584874889755 1105 86-87 0.0726028686951743 1105 88-89 0.15040953255268263 1105 90-91 0.3574636522042951 1105 92-93 0.36194217450148614 1105 94-95 0.2618443875111218 1105 96-97 0.3979233685061345 1105 98-99 0.7330492829904571 1105 100-101 0.8568392825130253 1106 1 0.13422824333666483 1106 2 0.15524616950030534 1106 3 0.11314661084746191 1106 4 0.09184404816103608 1106 5 -0.037345432406254986 1106 6 0.017273038734252566 1106 7 0.09831532803120524 1106 8 0.042886104646271406 1106 9 0.026930254002955678 1106 10-11 0.16654936797657882 1106 12-13 0.09385289885810266 1106 14-15 0.20575456848034435 1106 16-17 0.15918278285992926 1106 18-19 0.160868245556955 1106 20-21 0.22986224264061406 1106 22-23 0.1724686399463522 1106 24-25 0.10765655904089755 1106 26-27 0.11297999748241949 1106 28-29 0.08971756428100974 1106 30-31 0.20733994216651297 1106 32-33 0.16973880735006475 1106 34-35 0.2052506432271528 1106 36-37 0.01644664225296566 1106 38-39 0.16536171098979224 1106 40-41 0.3466876054883201 1106 42-43 0.10248502129129378 1106 44-45 0.18116087833168137 1106 46-47 0.13834820093222078 1106 48-49 0.2019765864908507 1106 50-51 0.22872829143230433 1106 52-53 0.17946756425759958 1106 54-55 0.18262856725424115 1106 56-57 0.24315567595768783 1106 58-59 0.2747632743259132 1106 60-61 0.06391948749876519 1106 62-63 0.06821681153396497 1106 64-65 0.16165240562573047 1106 66-67 0.2631726944221775 1106 68-69 0.2500401814044473 1106 70-71 0.2977137708854194 1106 72-73 0.19513171785679262 1106 74-75 0.323742658401148 1106 76-77 -0.02659672565821225 1106 78-79 0.21880715007985785 1106 80-81 0.3324771505057882 1106 82-83 0.47409243431305725 1106 84-85 0.2601805027159898 1106 86-87 0.37680317960104404 1106 88-89 0.40311919753807857 1106 90-91 0.4987211937260767 1106 92-93 0.6222959204215357 1106 94-95 0.8538262510092913 1106 96-97 0.5880026537422154 1106 98-99 0.9537150011344231 1106 100-101 1.303841392068378 1107 1 0.05242550760364395 1107 2 0.13356279274773897 1107 3 0.05585892704401374 1107 4 0.07665941895529471 1107 5 -0.00997171554421783 1107 6 0.02966037257053955 1107 7 0.06341146939583808 1107 8 -0.09005257848500037 1107 9 0.029192252555787945 1107 10-11 0.0998327809725339 1107 12-13 0.016453670251017627 1107 14-15 0.12324158448012668 1107 16-17 0.15051617959776564 1107 18-19 0.033461980335054875 1107 20-21 0.06423350343490597 1107 22-23 -0.022025482070475277 1107 24-25 -0.004797863616097686 1107 26-27 0.017211348525314918 1107 28-29 -0.05759878649840999 1107 30-31 -0.1460968497540378 1107 32-33 7.875971165773876E-4 1107 34-35 0.10644082718161485 1107 36-37 0.11556571591314224 1107 38-39 0.14841744612004248 1107 40-41 0.1856906617568157 1107 42-43 0.060374737294800696 1107 44-45 0.3176347374682962 1107 46-47 0.3830456779041711 1107 48-49 0.11649344416358787 1107 50-51 0.18176322517245325 1107 52-53 0.18425840650505876 1107 54-55 0.2203586388542078 1107 56-57 0.09766916633713407 1107 58-59 0.2858025662689556 1107 60-61 0.25298433102886975 1107 62-63 0.3962645975386252 1107 64-65 0.35629946959016223 1107 66-67 0.3205763699127786 1107 68-69 0.26470204475146275 1107 70-71 0.1318810019807053 1107 72-73 0.08643674322073736 1107 74-75 0.1679502391808434 1107 76-77 -0.0706438459155656 1107 78-79 0.01287354873396751 1107 80-81 0.24280882889586408 1107 82-83 0.2490963542537301 1107 84-85 0.13812334998096532 1107 86-87 0.14196788779680247 1107 88-89 0.41948446789188765 1107 90-91 0.27352622487353884 1107 92-93 0.2148625883305897 1107 94-95 0.6203232570742756 1107 96-97 0.6505483596078605 1107 98-99 1.0992815161015308 1107 100-101 1.0943846637151253 1108 1 -0.03708543343547532 1108 2 0.03137641415370496 1108 3 0.013021580209894523 1108 4 -0.07743343402022163 1108 5 -0.10401962723799585 1108 6 0.006118027832009432 1108 7 0.09520620673451674 1108 8 0.06490370292880954 1108 9 0.03978252888497735 1108 10-11 -0.08481630861670908 1108 12-13 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2316 86-87 -1.8560021321877436 2316 88-89 -1.5856138267173918 2316 90-91 -1.9173401819301468 2316 92-93 -1.775900543688742 2316 94-95 -2.0382314705803815 2316 96-97 -1.9384475285552867 2316 98-99 -2.099034801633639 2316 100-101 -2.18064789896804 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 12.0 4 35.0 5 116.0 6 205.0 7 481.0 8 1271.0 9 2151.0 10 3625.0 11 5363.0 12 7675.0 13 10192.0 14 13300.0 15 16944.0 16 20553.0 17 25153.0 18 22685.0 19 16888.0 20 16233.0 21 17268.0 22 18745.0 23 21282.0 24 25050.0 25 30222.0 26 36521.0 27 45184.0 28 55517.0 29 66801.0 30 81191.0 31 98383.0 32 119618.0 33 148085.0 34 188249.0 35 244708.0 36 326937.0 37 386925.0 38 198931.0 39 8271.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.29577065101018 19.692633475573356 15.257929015078847 22.75366685833762 2 16.086173754126023 20.9401889287606 38.947571041212846 24.026066275900533 3 19.65925742743567 24.45947806474074 30.029035378262314 25.852229129561277 4 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29.526673256634528 22.107566427467933 24-25 22.402739970479068 26.14674222938067 29.672658762703307 21.777859037436954 26-27 22.219886160471074 26.363499441939453 29.52292674093297 21.893687656656507 28-29 21.88792254856609 26.610272049038542 29.634929439130424 21.86687596326494 30-31 22.094284315623298 26.173139061023992 29.605539930825405 22.127036692527305 32-33 21.886664806180207 26.417818929653226 29.476748047968982 22.21876821619759 34-35 22.58637446595239 26.34184703574115 29.232034766617332 21.839743731689133 36-37 22.604696582659166 26.095726411025854 29.242143439870677 22.0574335664443 38-39 22.23410032608026 26.353502748321862 29.382258453849435 22.030138471748444 40-41 22.464772907886154 26.299343962205207 29.22506426101206 22.01081886889657 42-43 22.311909518038693 26.43497968224683 28.87725341157612 22.37585738813836 44-45 22.456397499678285 26.549881804049686 28.6560343291111 22.337686367160927 46-47 22.663707727032776 26.762561097733574 28.213604867123937 22.36012630810971 48-49 22.820095674269908 26.906475671810202 27.90675649931525 22.36667215460464 50-51 22.653281031904587 27.02021502981361 27.923096467899754 22.403407470382046 52-53 22.961265798530764 27.085791905636427 27.65070358557125 22.30223871026156 54-55 22.591649091730208 26.91753883659036 27.800487567468025 22.690324504211407 56-57 22.757996421335367 27.216748606482373 27.498148626388204 22.52710634579406 58-59 23.051900932544527 26.795364154494862 27.600998619313216 22.55173629364739 60-61 22.735805851269653 26.85077659799065 28.093802540421525 22.319615010318174 62-63 22.682719305149575 26.95044665729951 28.00770477538664 22.35912926216428 64-65 22.470653776055112 26.992409149536662 27.856380048097527 22.680557026310694 66-67 22.532607968851565 27.16069790206264 27.547817165181186 22.758876963904605 68-69 22.631020253896068 26.88421565863418 27.79323601513046 22.69152807233929 70-71 22.691456513089314 26.865897409759192 27.739636460892086 22.703009616259404 72-73 22.689385311481075 26.814120238580408 27.805793776188043 22.690700673750477 74-75 22.53519737231539 27.30675481739986 27.449719135733453 22.708328674551296 76-77 22.635610922184085 27.054683704591014 27.546502940304713 22.76320243292019 78-79 22.613224407329003 27.020724411713637 27.574766781310235 22.791284399647125 80-81 22.63142836785364 27.31945213287806 27.326511485149602 22.722608014118705 82-83 22.684844966474735 27.27547367701042 27.359922619162457 22.67975873735239 84-85 22.543107766841082 27.23490512904104 27.303964373322437 22.918022730795446 86-87 22.64696294162435 27.16876132197973 27.236175263972168 22.948100472423754 88-89 22.560342476385248 27.20318114012048 27.048088062349436 23.18838832114484 90-91 22.529464449172934 27.24004906813301 27.189402335669442 23.041084147024616 92-93 22.693877443643803 27.059744025893366 27.312403416700338 22.933975113762493 94-95 22.378859261240443 27.05607671278524 27.321563632457362 23.243500393516943 96-97 22.46112833868795 26.87839596096447 27.337391222376045 23.323084477971538 98-99 22.283319649623962 27.002083796916942 27.025585430740882 23.68901112271821 100-101 22.17916712614432 26.978328074278192 27.175879714268113 23.666625085309377 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 382.0 1 290.5 2 277.0 3 346.5 4 289.5 5 272.5 6 462.5 7 898.5 8 1430.0 9 1788.5 10 1762.0 11 1621.0 12 1658.0 13 1833.5 14 2139.5 15 2394.5 16 2577.0 17 2881.0 18 3153.5 19 3431.0 20 3920.5 21 4555.5 22 5348.0 23 6369.5 24 7653.0 25 9246.5 26 11661.5 27 14732.5 28 17747.5 29 21483.5 30 26178.5 31 31072.0 32 36509.0 33 40487.5 34 44317.0 35 48253.0 36 52745.0 37 58032.5 38 61189.5 39 62666.5 40 64933.5 41 69554.0 42 73471.0 43 76905.5 44 79851.0 45 83068.0 46 84974.0 47 87796.0 48 96472.0 49 96690.0 50 88443.5 51 82750.5 52 78264.0 53 76591.5 54 75029.5 55 69761.0 56 63062.5 57 56270.0 58 51096.5 59 45721.5 60 38650.5 61 32090.5 62 26765.5 63 20943.0 64 15362.5 65 12122.5 66 9415.0 67 7443.0 68 5534.5 69 3753.0 70 2876.0 71 2320.0 72 1964.5 73 1724.0 74 1411.5 75 1148.5 76 885.0 77 599.5 78 388.0 79 254.5 80 189.5 81 143.5 82 94.0 83 61.0 84 32.5 85 13.5 86 6.5 87 4.0 88 3.0 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.036128102182900274 2 0.005480598025318609 3 0.00469139190967273 4 5.699821946331354E-4 5 4.8229262622803763E-4 6 2.192239210127444E-4 7 0.0 8 0.0 9 0.0015345674470892104 10-11 4.384478420254888E-4 12-13 0.0010741972129624474 14-15 0.002170316818026169 16-17 0.0025868422679503835 18-19 0.0 20-21 2.1922392101274437E-5 22-23 0.0014688002707853873 24-25 8.111285077471542E-4 26-27 0.004669469517571455 28-29 0.005173684535900767 30-31 9.865076445573497E-4 32-33 3.288358815191166E-4 34-35 0.008527810527395756 36-37 0.010632360169118102 38-39 0.0087470344484085 40-41 0.001556489839190485 42-43 3.9460305782293984E-4 44-45 0.001161886781367545 46-47 4.3844784202548875E-5 48-49 5.919045867344098E-4 50-51 8.549732919497031E-4 52-53 0.0027402990126593045 54-55 0.002762221404760579 56-57 0.0014907226628866617 58-59 0.0010522748208611728 60-61 0.0038802634019255754 62-63 0.004801003870179101 64-65 0.00870318966420595 66-67 0.0035295051283051842 68-69 0.0033322035993937145 70-71 2.1922392101274437E-5 72-73 0.0014249554865828384 74-75 0.005875201083141549 76-77 0.0024991526995452856 78-79 0.0035514275204064586 80-81 0.00484484865438165 82-83 0.004603702341267632 84-85 0.005370986064812237 86-87 0.0038364186177230262 88-89 0.009075870329927618 90-91 0.01186001412678947 92-93 0.001512645054987936 94-95 0.0025429974837478347 96-97 0.0013372659181777407 98-99 0.0035295051283051842 100-101 0.004537935164963809 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2280773.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.908790379278265 #Duplication Level Percentage of deduplicated Percentage of total 1 78.13879198768122 45.2492292570466 2 13.030002288648944 15.091033423497757 3 3.9572608174788644 6.874805614665447 4 1.669288581786446 3.8666593026077605 5 0.8649190378085067 2.504320762774993 6 0.48811113226773295 1.695955514417059 7 0.3373718595381796 1.3675757405704627 8 0.23153238770652257 1.072620840456864 9 0.1745787455486736 0.9098679582589947 >10 0.9740299153268865 10.449233940876793 >50 0.08462716619902567 3.368559473128253 >100 0.04537866140735683 4.806209047950941 >500 0.0030425322974847614 1.1761961536472172 >1k 9.127596892452145E-4 0.9005038447050661 >5k 1.521266148742024E-4 0.6672291253958297 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7981 0.34992522272054255 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7167 0.31423556837966776 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4187 0.1835781114560721 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3642 0.159682704065683 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 4.3844784202548875E-5 8 0.0 0.0 0.0 0.0 4.3844784202548875E-5 9 0.0 0.0 0.0 1.753791368101955E-4 4.3844784202548875E-5 10-11 0.0 0.0 0.0 1.753791368101955E-4 4.3844784202548875E-5 12-13 0.0 0.0 0.0 2.1922392101274437E-4 4.3844784202548875E-5 14-15 0.0 0.0 0.0 2.6306870521529326E-4 4.3844784202548875E-5 16-17 0.0 0.0 0.0 2.6306870521529326E-4 4.3844784202548875E-5 18-19 0.0 0.0 0.0 2.6306870521529326E-4 4.3844784202548875E-5 20-21 0.0 0.0 0.0 2.6306870521529326E-4 4.3844784202548875E-5 22-23 0.0 0.0 0.0 3.069134894178421E-4 4.3844784202548875E-5 24-25 0.0 0.0 0.0 5.04215018329312E-4 4.3844784202548875E-5 26-27 0.0 0.0 0.0 6.357493709369586E-4 4.3844784202548875E-5 28-29 0.0 0.0 0.0 0.001052274820861173 4.3844784202548875E-5 30-31 0.0 0.0 0.0 0.002564919875849109 4.3844784202548875E-5 32-33 0.0 2.1922392101274437E-5 0.0 0.005765589122635176 4.3844784202548875E-5 34-35 0.0 4.3844784202548875E-5 0.0 0.009821231661370947 4.3844784202548875E-5 36-37 0.0 4.3844784202548875E-5 0.0 0.015038760981474263 4.3844784202548875E-5 38-39 0.0 4.3844784202548875E-5 0.0 0.019708230499045718 4.3844784202548875E-5 40-41 0.0 4.3844784202548875E-5 0.0 0.023895407390389138 4.3844784202548875E-5 42-43 0.0 4.3844784202548875E-5 0.0 0.030165211531353625 4.3844784202548875E-5 44-45 0.0 4.3844784202548875E-5 0.0 0.03650078284862193 4.3844784202548875E-5 46-47 0.0 4.3844784202548875E-5 0.0 0.046475471254701806 4.3844784202548875E-5 48-49 0.0 4.3844784202548875E-5 0.0 0.0586643212630104 4.3844784202548875E-5 50-51 0.0 4.3844784202548875E-5 0.0 0.07146699825015465 4.3844784202548875E-5 52-53 0.0 4.3844784202548875E-5 0.0 0.0908025480834787 4.3844784202548875E-5 54-55 0.0 4.3844784202548875E-5 0.0 0.12155966420156675 4.3844784202548875E-5 56-57 0.0 4.3844784202548875E-5 0.0 0.16858319525880042 4.3844784202548875E-5 58-59 0.0 4.3844784202548875E-5 0.0 0.21444483953466653 4.3844784202548875E-5 60-61 0.0 4.3844784202548875E-5 0.0 0.26620360728577547 6.576717630382332E-5 62-63 0.0 4.3844784202548875E-5 0.0 0.32344297306220304 8.768956840509775E-5 64-65 0.0 4.3844784202548875E-5 0.0 0.3844529902800498 8.768956840509775E-5 66-67 0.0 4.3844784202548875E-5 0.0 0.46729771003076587 1.5345674470892105E-4 68-69 0.0 4.3844784202548875E-5 0.0 0.5727005712536934 1.753791368101955E-4 70-71 0.0 4.3844784202548875E-5 0.0 0.6797476118842165 1.753791368101955E-4 72-73 0.0 4.3844784202548875E-5 0.0 0.7923410177163619 1.753791368101955E-4 74-75 0.0 4.3844784202548875E-5 0.0 0.9121688129419281 1.753791368101955E-4 76-77 0.0 4.3844784202548875E-5 0.0 1.0469696019726644 1.753791368101955E-4 78-79 0.0 4.3844784202548875E-5 0.0 1.177385912583146 1.753791368101955E-4 80-81 0.0 4.3844784202548875E-5 0.0 1.3234328887618365 1.753791368101955E-4 82-83 0.0 4.3844784202548875E-5 0.0 1.4808137416568856 1.753791368101955E-4 84-85 0.0 4.3844784202548875E-5 0.0 1.6796059932312422 1.753791368101955E-4 86-87 0.0 4.3844784202548875E-5 0.0 1.906853509753053 1.753791368101955E-4 88-89 0.0 4.3844784202548875E-5 0.0 2.157930666488949 1.753791368101955E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10115 0.0 28.233337 1 GTATCAA 14155 0.0 27.795473 1 GTACATG 10240 0.0 24.454807 1 TACATGG 10480 0.0 23.388329 2 ACATGGG 10335 0.0 22.887138 3 ATCAACG 17060 0.0 22.885765 3 TCAACGC 17165 0.0 22.772446 4 CAACGCA 17415 0.0 22.418755 5 TATCAAC 17510 0.0 22.353825 2 AACGCAG 17825 0.0 21.956383 6 GAGTACT 9540 0.0 21.809036 12-13 CCCGCGA 420 0.0 20.921839 20-21 CGCGGAA 390 0.0 20.70481 82-83 AGTACTT 9940 0.0 20.59689 12-13 GTAAGGT 880 0.0 20.509476 4 GTACTTT 10125 0.0 20.408213 14-15 TCGCGCT 425 0.0 20.118881 60-61 ATTCGCG 390 0.0 20.095625 80-81 TAAGGTG 950 0.0 19.998596 5 AGAGTAC 15590 0.0 19.912222 10-11 >>END_MODULE