FastQCFastQC Report
Thu 26 May 2016
SRR936452_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936452_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2327134
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75030.3224137501321368No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61000.2621250001074283No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34710.149153422192276No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33180.14257881153384377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA133550.028.4352051
GTACATG103900.027.7668511
GGTATCA92900.026.8082891
TACATGG107150.026.0807442
ACATGGG105400.025.1631953
CGTATAG1201.7046195E-423.7642171
TCAACGC158650.023.5779934
ATCAACG159950.023.4160793
TATCAAC163050.023.172942
CAACGCA161550.023.0679655
GAGTACT80200.022.64261412-13
AACGCAG164850.022.6061886
GTATAGG6250.022.0531921
CATGGGG48600.020.9290584
GTACTTT86350.020.77597414-15
ACGCAGA180550.020.5614517
AGTACTT86000.020.47987712-13
CGCAGAG182250.020.3996878
GTATAGC8900.020.2930371
CATGGGA57250.020.257534
Produced by FastQC (version 0.11.5)