##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936452_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2327134 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.309842492954854 31.0 31.0 34.0 28.0 34.0 2 31.364564739288756 31.0 31.0 34.0 28.0 34.0 3 31.309728618979396 31.0 31.0 34.0 28.0 34.0 4 34.98685937294543 37.0 35.0 37.0 32.0 37.0 5 35.02891539550365 37.0 35.0 37.0 32.0 37.0 6 35.09248113774282 37.0 35.0 37.0 32.0 37.0 7 34.95862034588468 37.0 35.0 37.0 32.0 37.0 8 34.94232949198456 37.0 35.0 37.0 32.0 37.0 9 36.42494201021514 39.0 37.0 39.0 32.0 39.0 10-11 36.294589396227295 39.0 35.0 39.0 32.0 39.0 12-13 36.18538855089565 39.0 35.0 39.0 32.0 39.0 14-15 37.158115518917256 39.0 36.0 41.0 31.0 41.0 16-17 37.065842362322066 39.0 36.0 41.0 31.0 41.0 18-19 36.93284873152986 39.0 36.0 41.0 31.0 41.0 20-21 36.919752364926126 39.0 36.0 41.0 31.0 41.0 22-23 36.92138398562352 39.0 36.0 41.0 30.5 41.0 24-25 37.04096691466843 39.0 36.0 41.0 31.0 41.0 26-27 36.969014891278285 39.0 36.0 41.0 31.0 41.0 28-29 36.853422278218616 39.0 36.0 41.0 30.0 41.0 30-31 36.652348124345224 39.0 36.0 41.0 30.0 41.0 32-33 36.483707427247424 39.0 35.0 40.5 30.0 41.0 34-35 36.28381047245239 39.0 35.0 40.0 30.0 41.0 36-37 36.08013956222547 38.5 35.0 40.0 29.5 41.0 38-39 35.884178349850075 38.0 35.0 40.0 29.0 41.0 40-41 35.64552556921947 38.0 35.0 40.0 27.5 41.0 42-43 35.36328183078413 38.0 34.0 40.0 27.0 41.0 44-45 35.205108730309476 38.0 34.0 40.0 26.0 41.0 46-47 35.04826322850339 38.0 34.0 40.0 25.5 41.0 48-49 34.806907982092994 38.0 34.0 40.0 24.0 41.0 50-51 33.831958752697524 37.5 33.0 39.5 23.5 40.5 52-53 33.94493183460858 37.5 33.0 39.5 23.5 40.5 54-55 34.38063235722567 38.0 34.0 40.0 24.0 41.0 56-57 34.241881859832745 38.0 33.5 40.0 23.0 41.0 58-59 34.0135243608662 38.0 33.0 40.0 23.0 41.0 60-61 33.71686782969954 37.0 33.0 40.0 22.0 41.0 62-63 33.377146954150476 37.0 32.5 40.0 21.5 41.0 64-65 33.02063031179124 36.0 32.0 39.0 20.0 41.0 66-67 32.6713401548858 36.0 32.0 39.0 19.5 41.0 68-69 32.23941573626615 35.0 31.0 39.0 18.0 40.0 70-71 31.847897671556517 35.0 31.0 38.0 17.5 40.0 72-73 31.426779463494583 35.0 31.0 37.5 13.5 39.5 74-75 31.01214519662383 35.0 30.5 37.0 11.0 39.0 76-77 30.55210916088201 34.5 30.0 36.0 7.5 39.0 78-79 30.0819609012631 34.0 29.5 36.0 7.0 38.5 80-81 29.63676973478966 34.0 29.0 35.5 3.5 37.0 82-83 29.241043704402067 34.0 29.0 35.0 2.0 37.0 84-85 28.860514263467422 34.0 29.0 35.0 2.0 36.0 86-87 28.49740045051123 34.0 28.5 35.0 2.0 36.0 88-89 28.135796004871228 34.0 27.0 35.0 2.0 36.0 90-91 27.774750186280635 33.5 27.0 35.0 2.0 35.0 92-93 27.342778284361792 33.0 26.0 35.0 2.0 35.0 94-95 26.83552322298587 33.0 25.0 35.0 2.0 35.0 96-97 26.213304863407092 33.0 24.5 35.0 2.0 35.0 98-99 25.43545730499404 32.5 21.0 35.0 2.0 35.0 100-101 22.78255420616088 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4701590843983894 1101 2 0.4111110018465318 1101 3 0.5215135309180852 1101 4 0.5699968778888049 1101 5 0.47430202492363094 1101 6 0.42907560462078465 1101 7 0.49058371246960775 1101 8 0.5413629477677944 1101 9 0.7188986168106837 1101 10-11 0.6766671455389854 1101 12-13 0.8049065150997592 1101 14-15 1.0197050857513545 1101 16-17 1.07463193353491 1101 18-19 1.1413099021792092 1101 20-21 1.0509124274242296 1101 22-23 0.9538331717173207 1101 24-25 0.9330051117316174 1101 26-27 0.9968081165899818 1101 28-29 0.848609093959034 1101 30-31 0.7348923334381539 1101 32-33 0.5906515973212692 1101 34-35 0.7674475303171917 1101 36-37 0.8690131911140568 1101 38-39 0.7618368803583948 1101 40-41 0.7099290857380964 1101 42-43 0.8487494333381349 1101 44-45 0.6064667991237087 1101 46-47 0.8420694734627716 1101 48-49 0.840800907003981 1101 50-51 0.7256370653367838 1101 52-53 0.8283429050556492 1101 54-55 0.7362004738981938 1101 56-57 0.8630854550268481 1101 58-59 0.8573322386343492 1101 60-61 0.7337397201306359 1101 62-63 0.7424330275068698 1101 64-65 0.5701399501286986 1101 66-67 0.5463612154432482 1101 68-69 0.6026199924878952 1101 70-71 0.474058846727214 1101 72-73 0.6581718025315588 1101 74-75 0.464069297721025 1101 76-77 0.5112988245283354 1101 78-79 0.47277149177276456 1101 80-81 0.5720922897048162 1101 82-83 0.41675782348764656 1101 84-85 0.4048964405286526 1101 86-87 0.5109298252558041 1101 88-89 0.5445242448455083 1101 90-91 0.48687150313440597 1101 92-93 0.6234120084711314 1101 94-95 0.7058479165554381 1101 96-97 0.6901967356370626 1101 98-99 0.6107843739535461 1101 100-101 1.0161299306900773 1102 1 0.42945113624756814 1102 2 0.5181422577116308 1102 3 0.6546851230471162 1102 4 0.5467538338747744 1102 5 0.5817508312049071 1102 6 0.6602834228233334 1102 7 0.6091587358763775 1102 8 0.560904342993922 1102 9 0.790223770970087 1102 10-11 0.7145945225108647 1102 12-13 0.623516787197488 1102 14-15 0.8716278732435754 1102 16-17 0.6982728837909988 1102 18-19 0.7200048390586673 1102 20-21 1.029027315992252 1102 22-23 0.6423315345836471 1102 24-25 0.8378572406131255 1102 26-27 0.6647490925497266 1102 28-29 0.5497304027221901 1102 30-31 0.5887942848786167 1102 32-33 0.6409399680774399 1102 34-35 0.3801621013610017 1102 36-37 0.4372890296378742 1102 38-39 0.3491513018991341 1102 40-41 0.5433521197209146 1102 42-43 0.4204840943483319 1102 44-45 0.5473902969521234 1102 46-47 0.44847163314220495 1102 48-49 0.5871842518902568 1102 50-51 0.4479850750190906 1102 52-53 0.20690723230792685 1102 54-55 0.5017606617529822 1102 56-57 0.3987681410199997 1102 58-59 0.21227029066870529 1102 60-61 0.22033636964604142 1102 62-63 0.2394064126002462 1102 64-65 0.35838924846168396 1102 66-67 0.3069039165868972 1102 68-69 0.37584257009667965 1102 70-71 0.18686852846299828 1102 72-73 0.4145962875735698 1102 74-75 0.24654269050047617 1102 76-77 0.3419833819910316 1102 78-79 0.38301861870058573 1102 80-81 0.49893257702811766 1102 82-83 0.41386231869671875 1102 84-85 0.5137474190015681 1102 86-87 0.38373201038864835 1102 88-89 0.38543887687741574 1102 90-91 0.5719010158695106 1102 92-93 0.40725513106186284 1102 94-95 0.6780602581016701 1102 96-97 0.4815181397075321 1102 98-99 0.7706840188569473 1102 100-101 1.3614343832146325 1103 1 0.39431952670939907 1103 2 0.41937766434910273 1103 3 0.4926902214423201 1103 4 0.4724116255192641 1103 5 0.40322199167871986 1103 6 0.4148622999541942 1103 7 0.522623258262854 1103 8 0.3560902958124288 1103 9 0.4585261187104095 1103 10-11 0.41015676106618315 1103 12-13 0.4394385694713634 1103 14-15 0.8004456184173492 1103 16-17 0.8723838140006066 1103 18-19 0.9363169896193924 1103 20-21 0.8503528714336284 1103 22-23 0.6188547287995902 1103 24-25 0.5732135223009962 1103 26-27 0.588495327387939 1103 28-29 0.5175487886946755 1103 30-31 0.5468181292816325 1103 32-33 0.6550274683377566 1103 34-35 0.42761850263959644 1103 36-37 0.4871831630011556 1103 38-39 0.35909622803317376 1103 40-41 0.5758904063337482 1103 42-43 0.7481459438156008 1103 44-45 0.6456638787369187 1103 46-47 0.8310128386835913 1103 48-49 0.9385117840095702 1103 50-51 0.8960357428392172 1103 52-53 0.9342511404699039 1103 54-55 0.9513802109953104 1103 56-57 0.9823264822082649 1103 58-59 0.8805260379707462 1103 60-61 0.7632240502995487 1103 62-63 0.9240611613897514 1103 64-65 0.772315081047644 1103 66-67 0.8367730905506008 1103 68-69 0.8972492410262305 1103 70-71 0.9024409610775628 1103 72-73 0.8699678330386966 1103 74-75 0.8720533749738593 1103 76-77 0.7145040423721554 1103 78-79 0.5964213997737744 1103 80-81 0.7991267113814509 1103 82-83 0.6373329281587594 1103 84-85 0.7629222669185651 1103 86-87 0.609659384715119 1103 88-89 0.6546124821160575 1103 90-91 0.9030784672659387 1103 92-93 0.7554510454495151 1103 94-95 0.8815787106803477 1103 96-97 0.975201049158958 1103 98-99 1.2192470354518221 1103 100-101 1.6285037994323375 1104 1 -0.03272656574126742 1104 2 0.05915691737679296 1104 3 0.2005127864508296 1104 4 0.3271007883309309 1104 5 0.2587895178565347 1104 6 0.1866679048696085 1104 7 0.1835620068491437 1104 8 0.15866493161890105 1104 9 0.4190555180634661 1104 10-11 0.2349152933688572 1104 12-13 0.31409327202095483 1104 14-15 0.32030212334687036 1104 16-17 0.32022389693942444 1104 18-19 0.21029547900872814 1104 20-21 0.4434985289455895 1104 22-23 0.19286321509549964 1104 24-25 0.27362583175527533 1104 26-27 0.026873968274813365 1104 28-29 -0.07525858133477215 1104 30-31 -0.1429395223552774 1104 32-33 -0.19684553752167488 1104 34-35 -0.07112422570075694 1104 36-37 0.11479105606876061 1104 38-39 0.028695843336002724 1104 40-41 0.030425769216556375 1104 42-43 0.19959206935495644 1104 44-45 0.233185527409006 1104 46-47 0.3364309477042582 1104 48-49 0.22508593170848457 1104 50-51 0.04679305783321297 1104 52-53 -3.379241117613674E-4 1104 54-55 0.15482321119907283 1104 56-57 0.29195688709907586 1104 58-59 0.2586337561386287 1104 60-61 0.24911177207561508 1104 62-63 0.22719848587947666 1104 64-65 0.2585820400306602 1104 66-67 0.2478378541021229 1104 68-69 0.11107743670407899 1104 70-71 0.090355463973399 1104 72-73 0.39335629281430684 1104 74-75 0.31042963123822176 1104 76-77 0.2521006586421528 1104 78-79 0.30035456324523935 1104 80-81 0.5054194177092661 1104 82-83 0.2723366868171979 1104 84-85 0.30066146716880127 1104 86-87 0.33855181636731757 1104 88-89 0.4371445302091246 1104 90-91 0.5641964736820491 1104 92-93 0.5408784364077199 1104 94-95 0.5440465778920682 1104 96-97 0.5138660585403905 1104 98-99 0.38963308281174136 1104 100-101 1.0980086712161174 1105 1 0.3050636154383959 1105 2 0.26982377185522566 1105 3 0.3264301695500116 1105 4 0.3237240181917187 1105 5 0.40900760893136834 1105 6 0.4174313116682882 1105 7 0.45188125696799375 1105 8 0.38214913862374544 1105 9 0.20366525108797617 1105 10-11 0.4420310649150565 1105 12-13 0.5233641035201799 1105 14-15 0.7229151345403366 1105 16-17 0.7791364188085552 1105 18-19 0.55921214660912 1105 20-21 0.5171487252675817 1105 22-23 0.4536362739248645 1105 24-25 0.2995504178624273 1105 26-27 0.2909752969896786 1105 28-29 0.48175426723079084 1105 30-31 0.2815136492891952 1105 32-33 0.39077233170051073 1105 34-35 0.18042177991118535 1105 36-37 0.3911609481356919 1105 38-39 0.2288687955608495 1105 40-41 0.28739812087060557 1105 42-43 0.19053300629022374 1105 44-45 0.3435321681661847 1105 46-47 0.6713215180064154 1105 48-49 0.6455139024290517 1105 50-51 0.7523040792357492 1105 52-53 0.8363178633450801 1105 54-55 0.5807389936298151 1105 56-57 0.5711355521095882 1105 58-59 0.5782947665765832 1105 60-61 0.4233842282533118 1105 62-63 0.562447525878575 1105 64-65 0.696031839066741 1105 66-67 0.6618457838135754 1105 68-69 0.7591246212083291 1105 70-71 0.8594233467212398 1105 72-73 0.7409259979231599 1105 74-75 0.7693203039831573 1105 76-77 0.838735157208756 1105 78-79 0.835837701003566 1105 80-81 0.9489274266538033 1105 82-83 0.7968958227123473 1105 84-85 0.7292872613004313 1105 86-87 0.8339386899257129 1105 88-89 0.7941923533800797 1105 90-91 0.6622679883465743 1105 92-93 0.7897073073442193 1105 94-95 0.8023489249522022 1105 96-97 1.070553491212273 1105 98-99 1.1787142831209891 1105 100-101 1.5090082246296248 1106 1 0.15858034519001762 1106 2 -0.056704597426271874 1106 3 0.19650752955114825 1106 4 0.31803800283972805 1106 5 0.2619530210995791 1106 6 0.17368919728792065 1106 7 0.34548875326105843 1106 8 0.27966869180222886 1106 9 0.2557879533032974 1106 10-11 0.43721150052497393 1106 12-13 0.45105555728498814 1106 14-15 0.29169850285068577 1106 16-17 0.2271109100627271 1106 18-19 0.4526760239574372 1106 20-21 0.09592115043017913 1106 22-23 -0.014969998567408993 1106 24-25 -0.26612457538693235 1106 26-27 -0.20303171104634288 1106 28-29 0.015137894829003073 1106 30-31 0.07026723581506644 1106 32-33 0.11836517080782016 1106 34-35 0.06468228051677016 1106 36-37 -0.11404825863611023 1106 38-39 0.08535099202272534 1106 40-41 0.03622990431944828 1106 42-43 0.1739498738970795 1106 44-45 0.044794577901001276 1106 46-47 0.1806557230017347 1106 48-49 0.07801655136561436 1106 50-51 -0.050553061365533836 1106 52-53 0.025400612807004563 1106 54-55 0.07328107263839456 1106 56-57 0.28049296984254113 1106 58-59 0.062106013858560516 1106 60-61 0.16191231961359875 1106 62-63 0.08935527340610605 1106 64-65 -0.1522013084067808 1106 66-67 -0.2561268756871442 1106 68-69 -0.08954641008465103 1106 70-71 -0.360293742117225 1106 72-73 -0.14286101020761421 1106 74-75 -0.02132338365234432 1106 76-77 -0.11570810817599764 1106 78-79 0.1368423230228224 1106 80-81 0.21203235928159714 1106 82-83 0.18523094832168496 1106 84-85 0.482671267882111 1106 86-87 0.5932715526879058 1106 88-89 0.524417074787749 1106 90-91 0.7370995380889447 1106 92-93 0.7496634400073852 1106 94-95 0.720311083452561 1106 96-97 0.7347521069751437 1106 98-99 1.137971033369304 1106 100-101 1.765726654122119 1107 1 0.18313863169201738 1107 2 0.20107602502352506 1107 3 0.3053281892269126 1107 4 0.2879312264815326 1107 5 0.3040809890381624 1107 6 0.21795296827465194 1107 7 0.35176971550815495 1107 8 0.3095267126278074 1107 9 0.4048201057605496 1107 10-11 0.486065753692813 1107 12-13 0.42347656935676525 1107 14-15 0.15885936997293015 1107 16-17 0.16155693011214822 1107 18-19 0.4349159824644673 1107 20-21 0.0794800650284131 1107 22-23 0.14887943122604952 1107 24-25 0.12162345594983037 1107 26-27 0.033691824537648074 1107 28-29 0.14723834792271617 1107 30-31 0.08078554854445485 1107 32-33 0.21480920816988913 1107 34-35 0.2828128344093557 1107 36-37 0.20477701750092336 1107 38-39 0.14731273057100935 1107 40-41 0.21126321732932496 1107 42-43 0.15999036633187558 1107 44-45 0.3944770352562159 1107 46-47 0.2910732920394423 1107 48-49 0.3891487982672004 1107 50-51 0.2576955065658737 1107 52-53 0.3867295191195055 1107 54-55 0.5246666087686407 1107 56-57 0.5407163164459021 1107 58-59 0.5638185646557332 1107 60-61 0.5879622656782431 1107 62-63 0.6069923165534661 1107 64-65 0.5132574455349115 1107 66-67 0.4808141485603059 1107 68-69 0.6043571481770229 1107 70-71 0.6656041917864535 1107 72-73 0.6693901767838959 1107 74-75 0.5955935559097156 1107 76-77 0.6549917087288968 1107 78-79 0.6869155242812219 1107 80-81 0.753833864321539 1107 82-83 0.8278906369588448 1107 84-85 0.8359526338396606 1107 86-87 1.003834512667229 1107 88-89 0.8376699511098096 1107 90-91 0.8941318707905062 1107 92-93 0.9271684450054885 1107 94-95 0.9824580322199559 1107 96-97 1.1887121019356606 1107 98-99 1.3075905799445238 1107 100-101 1.831565944253832 1108 1 0.1138315993932082 1108 2 0.043388299430489496 1108 3 0.2813118891533044 1108 4 0.3377648392260397 1108 5 0.31245726026571674 1108 6 0.19891382552120263 1108 7 0.27690524625411683 1108 8 0.2676719448656044 1108 9 -0.2039506063507801 1108 10-11 0.12551362041881475 1108 12-13 0.12687134516077947 1108 14-15 0.5047829988290644 1108 16-17 0.6566065943989159 1108 18-19 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98-99 -8.3261194971817 2316 100-101 -8.915727765017508 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5297.0 3 1985.0 4 2088.0 5 2336.0 6 2591.0 7 3129.0 8 4069.0 9 4859.0 10 6209.0 11 7987.0 12 10073.0 13 12033.0 14 13938.0 15 16204.0 16 19703.0 17 23898.0 18 19095.0 19 15392.0 20 15784.0 21 17037.0 22 18860.0 23 21214.0 24 24240.0 25 28240.0 26 32859.0 27 39562.0 28 47389.0 29 56795.0 30 68451.0 31 84208.0 32 104901.0 33 132553.0 34 173181.0 35 230958.0 36 315449.0 37 401024.0 38 302307.0 39 41223.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.28600915951751 18.508804747468233 14.37235803822916 22.832828054785097 2 15.613478934597639 19.885361570980617 40.38960731932197 24.111552175099778 3 19.637617657790695 24.363742167787176 29.4360548898494 26.562585284572727 4 12.731408157292003 16.40368095469981 37.33750981549238 33.52740107251581 5 13.92751087311115 37.37341566768145 33.748400570615374 14.950672888592035 6 31.884070430145194 35.79239071425225 17.6630716281093 14.660467227493253 7 28.953195398107244 32.62621210798528 20.256765421385047 18.16382707252243 8 26.638511342027005 34.1858896335924 19.65611694706624 19.519482077314354 9 26.632913656767666 16.924164520552598 20.395128661491338 36.047793161188395 10-11 25.14702708187682 26.455007004156055 27.074180419148647 21.323785494818473 12-13 26.357634683400377 23.7879756732155 27.530663720570836 22.323725922813292 14-15 23.03636831449318 25.2199761446659 26.164409208614657 25.579246332226262 16-17 22.275291182419572 28.595735551481155 26.64316183231083 22.485811433788445 18-19 22.13426559476379 26.936181537319907 29.160061858409247 21.769491009507057 20-21 23.103279028127936 25.978929190630957 29.153905530775866 21.763886250465244 22-23 22.576964384484768 26.050692295890148 29.065683122437765 22.306660197187323 24-25 22.5034067579849 26.2272118998094 29.23981534630305 22.02956599590265 26-27 22.2545016598551 26.4692033318741 29.138038007640898 22.1382570006299 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27.090180999694475 27.474366505366476 22.483865326017316 54-55 22.555285838841183 26.923643814083682 27.65701401681812 22.864056330257014 56-57 22.75587592157574 27.207457648823485 27.380014400141295 22.656652029459483 58-59 22.992070255141574 26.844569179750767 27.43808606650191 22.725274498605753 60-61 22.700226856374 26.926141254297875 27.936745790651834 22.436886098676293 62-63 22.641236601280227 26.992812476365312 27.875568091967303 22.490382830387162 64-65 22.450830876434875 27.131807929196633 27.69959103974396 22.717770154624535 66-67 22.489003078257173 27.182448240395836 27.45942048322643 22.86912819812056 68-69 22.54062459243267 26.96449332518861 27.68767757043784 22.807204511940878 70-71 22.59696969566751 26.919035895858052 27.572555114900865 22.911439293573572 72-73 22.641074357769252 26.92661702848726 27.631131893615525 22.801176720127962 74-75 22.482658437841135 27.406952956446247 27.36191217046734 22.748476435245273 76-77 22.674510565795742 27.113051693398504 27.378722442193727 22.83371529861203 78-79 22.664619674676125 27.135566514488975 27.366755692480144 22.833058118354764 80-81 22.59141352661261 27.452261257941235 27.213441304420233 22.74288391102592 82-83 22.667083218022523 27.352899214625936 27.272410835377414 22.707606731974128 84-85 22.578799405942636 27.36681838965075 27.10670342854982 22.947678775856794 86-87 22.651331739937934 27.322906648859142 27.172741594065762 22.853020017137165 88-89 22.61985769004258 27.287118944215415 26.98680999792231 23.106213367819695 90-91 22.568878620983657 27.30282269986317 27.15043713085391 22.977861548299263 92-93 22.74295296619839 27.203821735432665 27.22169959100108 22.83152570736787 94-95 22.50111309950508 27.13908500642074 27.264448375390444 23.09535351868374 96-97 22.548921858252747 27.02423938726377 27.308268518192218 23.118570236291262 98-99 22.402481368354387 27.16241310622558 26.97680046092625 23.458305064493786 100-101 22.47361286490492 27.059739493927452 27.139100752911204 23.327546888256425 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 304.0 1 227.5 2 242.5 3 335.5 4 287.0 5 276.5 6 469.0 7 874.5 8 1384.5 9 1713.5 10 1676.5 11 1553.0 12 1597.5 13 1831.5 14 2107.5 15 2251.0 16 2408.0 17 2672.5 18 2896.5 19 3154.5 20 3516.0 21 4091.0 22 4885.5 23 5937.5 24 7319.5 25 9050.0 26 11389.5 27 14376.5 28 17582.5 29 21369.0 30 26043.0 31 31344.5 32 36997.0 33 41125.0 34 45134.5 35 49487.5 36 53937.5 37 58750.5 38 61809.0 39 63787.0 40 66314.0 41 70644.0 42 74922.5 43 78469.0 44 81986.5 45 85535.5 46 86681.5 47 89570.5 48 98431.5 49 98363.0 50 90092.5 51 84866.5 52 80433.5 53 78158.5 54 76442.0 55 71516.5 56 65137.5 57 58317.5 58 52801.0 59 46900.5 60 39910.0 61 33487.0 62 27958.0 63 21964.0 64 16395.5 65 12988.0 66 9906.5 67 7827.0 68 5898.5 69 4045.5 70 3132.0 71 2538.0 72 2144.5 73 1898.5 74 1527.0 75 1207.0 76 886.0 77 555.0 78 367.5 79 265.0 80 200.0 81 142.0 82 95.5 83 54.0 84 35.5 85 18.0 86 14.0 87 12.5 88 5.5 89 4.5 90 5.0 91 3.5 92 4.0 93 4.0 94 2.5 95 2.0 96 1.5 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07236368855424741 2 0.029607233618691487 3 0.01568452869495268 4 0.01955194672932457 5 0.02247399591085 6 0.029521290995705447 7 0.07489899593233566 8 0.052725799201936804 9 0.06931272543824292 10-11 0.05895663936842485 12-13 0.03830892419602824 14-15 0.04423896518206515 16-17 0.052833227480669355 18-19 0.05057723362728575 20-21 0.01753229508915258 22-23 0.013364077874329541 24-25 0.021850911894201193 26-27 0.024729989764233604 28-29 0.005887069674543881 30-31 6.015983609022944E-4 32-33 0.009088432380773948 34-35 0.010742827873255256 36-37 0.00416821721482304 38-39 0.009453688528464626 40-41 0.003996331968850956 42-43 0.001461024590762715 44-45 8.594262298604205E-5 46-47 2.578278689581262E-4 48-49 5.156557379162524E-4 50-51 9.668545085929731E-4 52-53 1.289139344790631E-4 54-55 4.2971311493021026E-5 56-57 0.0031583913947370455 58-59 0.003136905738990535 60-61 0.004769815575725335 62-63 0.001976680328678967 64-65 5.586270494092735E-4 66-67 2.3634221321161566E-4 68-69 0.0027716495912998565 70-71 0.0017618237712138621 72-73 0.021378227467777962 74-75 0.015211844268529446 76-77 0.024493647551021985 78-79 0.02011057377873384 80-81 0.011881567627820316 82-83 0.0038674180343718926 84-85 0.0036955327883998086 86-87 0.0012031967218045889 88-89 0.0013106250005371415 90-91 0.006101926232008986 92-93 0.010012315577873899 94-95 0.012805450824920265 96-97 0.011172540988185467 98-99 0.02210873976315932 100-101 0.018563606564985086 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2327134.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.412840593002635 #Duplication Level Percentage of deduplicated Percentage of total 1 79.18810207362425 47.047900853626594 2 12.42412721623817 14.763053796110881 3 3.77322947908182 6.725348446845198 4 1.5489814484872255 3.6811755152195946 5 0.8242093033998859 2.4484307979083586 6 0.49209835781922767 1.7542176773515286 7 0.32151532850326375 1.3371497272399901 8 0.23025273228274093 1.094397510337424 9 0.17029620751141228 0.9106003287421593 >10 0.9039847646284827 10.002876552178384 >50 0.0767953004411085 3.150275100710627 >100 0.04306034544270642 4.7379067727504065 >500 0.0026197376397216303 1.0982990915790274 >1k 5.821639199379691E-4 0.6602419173916366 >5k 1.4554097998449228E-4 0.5881259120082794 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7503 0.3224137501321368 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6100 0.2621250001074283 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3471 0.149153422192276 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3318 0.14257881153384377 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 8.594262298604206E-5 0.0 0.0 0.0 5 0.0 8.594262298604206E-5 0.0 0.0 0.0 6 0.0 8.594262298604206E-5 0.0 0.0 0.0 7 0.0 8.594262298604206E-5 0.0 0.0 0.0 8 0.0 8.594262298604206E-5 0.0 0.0 0.0 9 0.0 8.594262298604206E-5 0.0 0.0 0.0 10-11 0.0 8.594262298604206E-5 0.0 0.0 0.0 12-13 0.0 8.594262298604206E-5 0.0 0.0 0.0 14-15 0.0 8.594262298604206E-5 0.0 0.0 0.0 16-17 0.0 8.594262298604206E-5 0.0 0.0 0.0 18-19 0.0 8.594262298604206E-5 0.0 0.0 0.0 20-21 0.0 8.594262298604206E-5 0.0 0.0 0.0 22-23 0.0 8.594262298604206E-5 0.0 6.445696723953155E-5 0.0 24-25 0.0 8.594262298604206E-5 0.0 1.289139344790631E-4 0.0 26-27 0.0 8.594262298604206E-5 0.0 3.437704919441682E-4 0.0 28-29 0.0 8.594262298604206E-5 0.0 0.0011387397545650573 0.0 30-31 0.0 8.594262298604206E-5 0.0 0.0029435348372719404 0.0 32-33 0.0 8.594262298604206E-5 0.0 0.005242500002148565 0.0 34-35 0.0 8.594262298604206E-5 0.0 0.008981004102041395 0.0 36-37 0.0 8.594262298604206E-5 0.0 0.013664877054780688 0.0 38-39 0.0 8.594262298604206E-5 0.0 0.01862806353222462 0.0 40-41 0.0 8.594262298604206E-5 0.0 0.023677192632654588 0.0 42-43 0.0 8.594262298604206E-5 0.0 0.03050963116004493 0.0 44-45 0.0 8.594262298604206E-5 0.0 0.038115553294309656 0.0 46-47 0.0 8.594262298604206E-5 0.0 0.04950295083996023 0.0 48-49 0.0 8.594262298604206E-5 0.0 0.062372858632120026 0.0 50-51 0.0 8.594262298604206E-5 0.0 0.07526425208002634 0.0 52-53 0.0 8.594262298604206E-5 0.0 0.09507402667830903 0.0 54-55 0.0 8.594262298604206E-5 0.0 0.12633565578948183 0.0 56-57 0.0 8.594262298604206E-5 0.0 0.1712191906439423 0.0 58-59 0.0 8.594262298604206E-5 0.0 0.21801494885984218 0.0 60-61 0.0 8.594262298604206E-5 0.0 0.2657775615843351 0.0 62-63 0.0 8.594262298604206E-5 0.0 0.3191479304586672 0.0 64-65 0.0 8.594262298604206E-5 0.0 0.3822942726976616 0.0 66-67 0.0 8.594262298604206E-5 0.0 0.46849472355266175 0.0 68-69 0.0 8.594262298604206E-5 0.0 0.5768898567938073 0.0 70-71 0.0 8.594262298604206E-5 0.0 0.6812242010988624 0.0 72-73 0.0 8.594262298604206E-5 0.0 0.7922190986853357 8.594262298604206E-5 74-75 0.0 8.594262298604206E-5 0.0 0.9107339757830877 8.594262298604206E-5 76-77 0.0 8.594262298604206E-5 0.0 1.0429567012471135 8.594262298604206E-5 78-79 0.0 8.594262298604206E-5 0.0 1.1769412504823529 8.594262298604206E-5 80-81 0.0 8.594262298604206E-5 0.0 1.3277705538228568 8.594262298604206E-5 82-83 0.0 8.594262298604206E-5 0.0 1.4941554719238344 8.594262298604206E-5 84-85 0.0 8.594262298604206E-5 0.0 1.6897393961843195 8.594262298604206E-5 86-87 0.0 8.594262298604206E-5 0.0 1.9118581052917452 8.594262298604206E-5 88-89 0.0 8.594262298604206E-5 0.0 2.1603611996558856 8.594262298604206E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13355 0.0 28.435205 1 GTACATG 10390 0.0 27.766851 1 GGTATCA 9290 0.0 26.808289 1 TACATGG 10715 0.0 26.080744 2 ACATGGG 10540 0.0 25.163195 3 CGTATAG 120 1.7046195E-4 23.764217 1 TCAACGC 15865 0.0 23.577993 4 ATCAACG 15995 0.0 23.416079 3 TATCAAC 16305 0.0 23.17294 2 CAACGCA 16155 0.0 23.067965 5 GAGTACT 8020 0.0 22.642614 12-13 AACGCAG 16485 0.0 22.606188 6 GTATAGG 625 0.0 22.053192 1 CATGGGG 4860 0.0 20.929058 4 GTACTTT 8635 0.0 20.775974 14-15 ACGCAGA 18055 0.0 20.561451 7 AGTACTT 8600 0.0 20.479877 12-13 CGCAGAG 18225 0.0 20.399687 8 GTATAGC 890 0.0 20.293037 1 CATGGGA 5725 0.0 20.25753 4 >>END_MODULE