FastQCFastQC Report
Thu 26 May 2016
SRR936452_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936452_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2327134
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79440.34136409850055904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71970.3092645288152724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42980.1846906967970044No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38370.1648809221987217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106050.028.2209191
GTATCAA145100.026.191711
CGTATAG1000.001653096623.7526071
GTACATG102750.023.348061
TACATGG105050.022.2475572
GAGTACT96400.022.02167912-13
ATCAACG175800.021.4785793
TATCAAC177350.021.4275282
TCAACGC176050.021.391824
CAACGCA177900.021.2227575
AACGCAG182400.020.6726886
ACATGGG105600.020.644583
GTACTTT102000.020.62640214-15
AGTACTT99400.019.46978412-13
TATACCG1759.9569355E-518.9963685
AGAGTAC161850.018.82284410-11
ACGCAGA200400.018.7921627
CGCAGAG202500.018.597288
TACCTGG19350.018.1661952
TAGCCCT15700.018.1493974
Produced by FastQC (version 0.11.5)