##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936452_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2327134 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.640683346983888 31.0 31.0 34.0 30.0 34.0 2 31.803316439878408 31.0 31.0 34.0 30.0 34.0 3 31.878285908761594 31.0 31.0 34.0 30.0 34.0 4 35.622164430582856 37.0 35.0 37.0 33.0 37.0 5 35.428124465544315 37.0 35.0 37.0 33.0 37.0 6 35.51482467275198 37.0 35.0 37.0 33.0 37.0 7 35.4554950423998 37.0 35.0 37.0 33.0 37.0 8 35.41721361984312 37.0 35.0 37.0 33.0 37.0 9 36.99970134938513 39.0 37.0 39.0 33.0 39.0 10-11 36.8744135490264 39.0 37.0 39.0 33.0 39.0 12-13 36.756087960555774 39.0 36.0 39.0 32.0 39.0 14-15 37.81533658998579 39.5 37.0 41.0 33.0 41.0 16-17 37.695822844752385 39.0 37.0 41.0 32.0 41.0 18-19 37.85546212637519 39.5 37.0 41.0 32.5 41.0 20-21 37.876182033350894 40.0 37.0 41.0 32.5 41.0 22-23 37.78295964048482 39.5 37.0 41.0 32.0 41.0 24-25 37.637207397597216 39.0 37.0 41.0 32.0 41.0 26-27 37.41418672066155 39.0 36.0 41.0 32.0 41.0 28-29 37.31579444930975 39.0 36.0 41.0 31.5 41.0 30-31 37.27584187244912 39.0 36.0 41.0 31.0 41.0 32-33 37.10930333190955 39.0 36.0 41.0 31.0 41.0 34-35 36.92470459371914 39.0 35.5 41.0 31.0 41.0 36-37 36.70789563471635 39.0 35.0 40.0 30.0 41.0 38-39 36.52862533915108 39.0 35.0 40.0 30.0 41.0 40-41 36.316403782506725 39.0 35.0 40.0 30.0 41.0 42-43 36.019720609126935 38.0 35.0 40.0 29.5 41.0 44-45 35.736569746305975 38.0 35.0 40.0 28.5 41.0 46-47 35.352807143894594 38.0 34.5 40.0 27.0 41.0 48-49 34.96512254988325 38.0 34.0 40.0 26.0 41.0 50-51 34.6577526261917 38.0 33.5 40.0 25.0 41.0 52-53 34.279454685462895 38.0 33.0 40.0 24.0 41.0 54-55 34.07167657728347 38.0 33.0 40.0 24.0 41.0 56-57 33.74787893606471 37.0 33.0 40.0 23.0 41.0 58-59 33.35119314143492 37.0 32.0 39.5 22.5 41.0 60-61 32.920709765746196 36.0 31.5 39.0 21.0 40.0 62-63 32.56920830515132 36.0 31.0 39.0 20.0 40.0 64-65 32.127095818289796 35.0 31.0 38.5 19.0 40.0 66-67 31.688760724565064 35.0 30.5 38.0 18.0 40.0 68-69 31.314783764063435 35.0 30.0 38.0 17.0 40.0 70-71 30.918861569638878 34.0 29.5 37.0 13.5 39.0 72-73 30.433844806530267 34.0 29.0 36.5 9.5 39.0 74-75 29.913262622607895 34.0 29.0 36.0 7.5 39.0 76-77 28.473802969661392 32.0 27.0 34.5 7.0 36.5 78-79 29.242226919464027 33.5 29.0 35.0 7.0 37.0 80-81 29.01585662879748 34.0 29.0 35.0 2.0 37.0 82-83 28.681430248537474 33.0 28.0 35.0 2.0 36.5 84-85 28.27008715441397 33.0 27.0 35.0 2.0 36.0 86-87 27.83300445956271 33.0 26.5 35.0 2.0 35.5 88-89 27.313523630353906 32.5 26.0 35.0 2.0 35.0 90-91 26.778325829109967 32.0 25.0 34.0 2.0 35.0 92-93 26.095763931084328 32.0 24.5 34.0 2.0 35.0 94-95 25.464633751215015 31.5 21.5 34.0 2.0 35.0 96-97 24.624585004559258 31.0 18.5 34.0 2.0 35.0 98-99 23.587360461408757 31.0 2.0 34.0 2.0 35.0 100-101 20.276713975215866 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3107325368718534 1101 2 0.32926303523251477 1101 3 0.26285796497671754 1101 4 0.30734192084786116 1101 5 0.19800070025409156 1101 6 0.28342382590528103 1101 7 0.3583758066180138 1101 8 0.3384322562319255 1101 9 0.29368984120403496 1101 10-11 0.3361469565314721 1101 12-13 0.32314032265555426 1101 14-15 0.47647633994854033 1101 16-17 0.4866105364254736 1101 18-19 0.41658462964505105 1101 20-21 0.4059383098372038 1101 22-23 0.25010457634299854 1101 24-25 0.41761265177260043 1101 26-27 0.35657514737709306 1101 28-29 0.23780361823382634 1101 30-31 0.3695718811545845 1101 32-33 0.31815348587620207 1101 34-35 0.4241141919808413 1101 36-37 0.4593660809493514 1101 38-39 0.38176351798271924 1101 40-41 0.4918200066411771 1101 42-43 0.5114470593043592 1101 44-45 0.4336565378081829 1101 46-47 0.5371812578521471 1101 48-49 0.45637458771344086 1101 50-51 0.3601951227101665 1101 52-53 0.45612419576896457 1101 54-55 0.3864738265425842 1101 56-57 0.3694953152915801 1101 58-59 0.23592551212448853 1101 60-61 0.4072213581654083 1101 62-63 0.4697353176682668 1101 64-65 0.48614815979652093 1101 66-67 0.2895010356308987 1101 68-69 0.13173037322877335 1101 70-71 0.1826333314641957 1101 72-73 0.33804397136198006 1101 74-75 0.37835620840522566 1101 76-77 0.16971719742421243 1101 78-79 0.2080023045858823 1101 80-81 0.381626098265901 1101 82-83 0.3918037774724965 1101 84-85 0.33464896867149463 1101 86-87 0.2268736470027406 1101 88-89 0.23785842377873934 1101 90-91 0.37243143858636785 1101 92-93 0.31157978586381674 1101 94-95 0.4089784601989592 1101 96-97 0.48713837759049383 1101 98-99 0.6626492770053787 1101 100-101 1.208231245289813 1102 1 0.37053197682520533 1102 2 0.3654286926402648 1102 3 0.38767336515125095 1102 4 0.33759269776690104 1102 5 0.3526745301030161 1102 6 0.3040132083274827 1102 7 0.3292579211962803 1102 8 0.3342124230876422 1102 9 0.5353961209406464 1102 10-11 0.5937641235734432 1102 12-13 0.5727183035919481 1102 14-15 0.6971182200407924 1102 16-17 0.7167595533051667 1102 18-19 0.615472687166907 1102 20-21 0.45379752384859984 1102 22-23 0.43656612970919184 1102 24-25 0.47947678558607265 1102 26-27 0.4925353542504425 1102 28-29 0.5805919541345474 1102 30-31 0.5314899247306926 1102 32-33 0.6121315008458126 1102 34-35 0.6886182597012223 1102 36-37 0.7765851737281224 1102 38-39 0.7094628402140231 1102 40-41 0.7590494130582357 1102 42-43 0.8979366221526632 1102 44-45 0.827073040415911 1102 46-47 0.6998693387539632 1102 48-49 0.9058781567327685 1102 50-51 0.8787864834919645 1102 52-53 0.9383951945688622 1102 54-55 0.7518465278256627 1102 56-57 0.6825893102516574 1102 58-59 0.9277928776637765 1102 60-61 1.048575758728525 1102 62-63 1.0327071398075205 1102 64-65 0.8255715148069598 1102 66-67 0.6777669752069215 1102 68-69 0.8543925288256453 1102 70-71 0.8774249798838838 1102 72-73 0.8815961242311943 1102 74-75 0.7780243487722309 1102 76-77 0.7598507098847698 1102 78-79 1.0434603606098563 1102 80-81 1.1544557030193978 1102 82-83 0.9644302017644932 1102 84-85 0.93635413303371 1102 86-87 0.9102644352256704 1102 88-89 0.9657025123122978 1102 90-91 0.9413959850526155 1102 92-93 0.9796400747100371 1102 94-95 0.8961971454370854 1102 96-97 1.1166660558564594 1102 98-99 1.1184392498610265 1102 100-101 1.831056667389312 1103 1 0.2946671460906316 1103 2 0.367329580848029 1103 3 0.3436149551718586 1103 4 0.2953424872114425 1103 5 0.3654799208690278 1103 6 0.285319775370489 1103 7 0.23500469407325397 1103 8 0.21026003678674243 1103 9 0.393109107987776 1103 10-11 0.3771227827371533 1103 12-13 0.3348924547214409 1103 14-15 0.44749819284525216 1103 16-17 0.47748687177939075 1103 18-19 0.35693345557229605 1103 20-21 0.32979802501667876 1103 22-23 0.26184819891886235 1103 24-25 0.3784490837592358 1103 26-27 0.20339704585659746 1103 28-29 0.22645695898064844 1103 30-31 0.3002891048360965 1103 32-33 0.238084974831672 1103 34-35 0.30065868634957127 1103 36-37 0.206420788548364 1103 38-39 0.24989870623281263 1103 40-41 0.368688124810447 1103 42-43 0.4778705417069915 1103 44-45 0.34425136168718495 1103 46-47 0.4073368797563788 1103 48-49 0.28944585214821217 1103 50-51 0.38221426151820026 1103 52-53 0.29700989156189195 1103 54-55 0.38307643458723106 1103 56-57 0.2434865240234032 1103 58-59 0.2570267664584023 1103 60-61 0.3288748345377144 1103 62-63 0.45040666905774174 1103 64-65 0.24814061916018915 1103 66-67 0.209881334389447 1103 68-69 0.293629824743892 1103 70-71 0.14158758707769437 1103 72-73 0.21844440515297592 1103 74-75 0.31444967523988154 1103 76-77 0.3352949417917621 1103 78-79 0.11903304244288648 1103 80-81 0.22776100894871476 1103 82-83 0.2662166884567405 1103 84-85 0.7256423138993995 1103 86-87 0.6848057110944623 1103 88-89 0.6129062128991905 1103 90-91 0.6499210828879356 1103 92-93 0.6741286255115106 1103 94-95 0.741772595984802 1103 96-97 0.781869389102166 1103 98-99 0.7015093106327761 1103 100-101 1.178264242811185 1104 1 0.15571354804885118 1104 2 0.13446214793362898 1104 3 0.1693013523147826 1104 4 0.12931832555837275 1104 5 0.12278126495738917 1104 6 0.06409719421233007 1104 7 0.047898002608711465 1104 8 0.1436206918639229 1104 9 0.15984813013137966 1104 10-11 0.1594648833132979 1104 12-13 0.18789371594006354 1104 14-15 0.31136510985891164 1104 16-17 0.3147960012490927 1104 18-19 0.13930080025566127 1104 20-21 0.09916424333594165 1104 22-23 0.15537718861022398 1104 24-25 0.2230527734774128 1104 26-27 0.182721464210168 1104 28-29 0.17794641886693796 1104 30-31 0.27056453860303975 1104 32-33 0.1517823616307652 1104 34-35 0.1383262492505679 1104 36-37 0.11374909531672728 1104 38-39 0.07118703718947472 1104 40-41 0.20566439386954727 1104 42-43 -0.058410420766122684 1104 44-45 -0.012671041216329115 1104 46-47 -0.009583529931639134 1104 48-49 0.09602221343463668 1104 50-51 0.026525777419436736 1104 52-53 0.13766941565372548 1104 54-55 0.08426127950536255 1104 56-57 0.031174125976292544 1104 58-59 -0.1345304693332352 1104 60-61 0.08698144097812843 1104 62-63 -0.10257668282356747 1104 64-65 -0.020059436524853425 1104 66-67 0.017282528378160578 1104 68-69 -0.010027265223971682 1104 70-71 0.020224691442095377 1104 72-73 0.1510015246737062 1104 74-75 0.03226142392961506 1104 76-77 -0.22472378654003577 1104 78-79 -0.1634608516900684 1104 80-81 0.2707739252154049 1104 82-83 0.14880642796879684 1104 84-85 0.48448165727632286 1104 86-87 0.5377015555526086 1104 88-89 0.6203768240713607 1104 90-91 0.41393596161672264 1104 92-93 0.34172063534865416 1104 94-95 0.43020826224093156 1104 96-97 0.71247292719125 1104 98-99 0.6283633998574594 1104 100-101 1.3432593165099966 1105 1 0.04549584113444283 1105 2 0.09359260081075149 1105 3 0.1107978812652739 1105 4 0.07322498097767749 1105 5 0.14773216445301784 1105 6 0.16486471472889974 1105 7 0.07716238341413373 1105 8 0.2233903236621586 1105 9 0.07573252345402892 1105 10-11 0.1476858844033515 1105 12-13 0.1791463030204241 1105 14-15 0.19351013362083336 1105 16-17 0.20384822945959513 1105 18-19 0.18876201525905145 1105 20-21 0.2211614293608264 1105 22-23 0.3153565559940432 1105 24-25 0.24293587666130634 1105 26-27 0.13657371290922526 1105 28-29 0.07693103484257335 1105 30-31 0.23660507713192658 1105 32-33 0.2733345618295644 1105 34-35 0.3916421939890924 1105 36-37 0.19360068133904917 1105 38-39 0.23190551322506536 1105 40-41 0.22285557290591385 1105 42-43 0.24640230902984683 1105 44-45 0.22396337731684923 1105 46-47 0.12192722896127606 1105 48-49 0.33638066712489945 1105 50-51 0.32348674832088875 1105 52-53 0.35506379954843936 1105 54-55 0.217739419976823 1105 56-57 0.1383215107241682 1105 58-59 0.08433700972602054 1105 60-61 0.24584722332884468 1105 62-63 0.15647229603000312 1105 64-65 0.27689217046622616 1105 66-67 0.3116580996167073 1105 68-69 0.48296580868369077 1105 70-71 0.38569515190638626 1105 72-73 0.4174312657335726 1105 74-75 0.34776805950062695 1105 76-77 0.2005434850725898 1105 78-79 0.16525225890491413 1105 80-81 0.12309673522086584 1105 82-83 0.31581232963333505 1105 84-85 0.37845129899870855 1105 86-87 0.41270255597038386 1105 88-89 0.1362893891585415 1105 90-91 0.4565026974856181 1105 92-93 0.3211201926037681 1105 94-95 0.6590521474473903 1105 96-97 0.7843311751956143 1105 98-99 0.7907260825137321 1105 100-101 1.4143682916180005 1106 1 0.09905425906794108 1106 2 0.11040146523415117 1106 3 0.05738458241943789 1106 4 0.11918288538856814 1106 5 0.20822961184142486 1106 6 -0.036397300231477914 1106 7 -0.0048467974604662345 1106 8 0.047279230973856556 1106 9 0.07500155908321915 1106 10-11 0.07963324359915447 1106 12-13 0.160545241550885 1106 14-15 0.2876831048354589 1106 16-17 0.13704497083338651 1106 18-19 0.22461156197902454 1106 20-21 0.05879733046509017 1106 22-23 0.10484473881567169 1106 24-25 0.062016668041955825 1106 26-27 0.031130404888827456 1106 28-29 -0.11308377709477213 1106 30-31 -0.019049119399426218 1106 32-33 0.10412112623385639 1106 34-35 0.14569621176657677 1106 36-37 -0.0900326016210613 1106 38-39 0.12037667810972152 1106 40-41 0.16956970725239273 1106 42-43 0.2813630605540709 1106 44-45 0.2289608270739052 1106 46-47 0.22490919724351954 1106 48-49 0.11479597932043362 1106 50-51 0.25864078141562175 1106 52-53 -0.03811457691794118 1106 54-55 0.20027065058353344 1106 56-57 -0.18642476773709404 1106 58-59 -0.11083134578574771 1106 60-61 0.03304160868871264 1106 62-63 0.08931958306102672 1106 64-65 0.10420400912033045 1106 66-67 0.09708192157027185 1106 68-69 -0.09657334741486068 1106 70-71 3.928415283951381E-4 1106 72-73 0.05486906901904476 1106 74-75 0.1288931035179779 1106 76-77 0.07304736570543113 1106 78-79 0.02488025504559843 1106 80-81 0.1464459069051145 1106 82-83 0.11146017819153897 1106 84-85 0.4789302393609205 1106 86-87 0.61668132973778 1106 88-89 0.44068670279736466 1106 90-91 0.60758572171056 1106 92-93 0.7215869739129346 1106 94-95 0.680978078286067 1106 96-97 1.0080560932009632 1106 98-99 1.062003083112213 1106 100-101 1.596622469516003 1107 1 -0.03952648256380442 1107 2 -0.020005289145281324 1107 3 -0.0066062839763105785 1107 4 0.04542250179926555 1107 5 -0.04254493288077299 1107 6 -0.12993535798874234 1107 7 -0.051759515995193794 1107 8 0.039056044383301014 1107 9 0.04598176623542116 1107 10-11 0.0919431939124209 1107 12-13 -0.07713653161165723 1107 14-15 0.18464520663118833 1107 16-17 0.13347250112775555 1107 18-19 -0.02072162789455234 1107 20-21 -0.042833788530181494 1107 22-23 -0.17248388425578298 1107 24-25 -0.12597055484391717 1107 26-27 -0.08060290159178152 1107 28-29 -0.023296824023589124 1107 30-31 0.05288858964028975 1107 32-33 0.10094661939974969 1107 34-35 0.09639410667863046 1107 36-37 0.020609583087050964 1107 38-39 -0.1543289765035709 1107 40-41 0.07278840152955013 1107 42-43 -0.05416125265536209 1107 44-45 0.07336700025741294 1107 46-47 0.2003550490036119 1107 48-49 0.17810472218077678 1107 50-51 0.21880816951930626 1107 52-53 0.34512366834857033 1107 54-55 0.3144554175487997 1107 56-57 0.05333878116290691 1107 58-59 0.10594323756023272 1107 60-61 0.18004762204334668 1107 62-63 0.1896866928895875 1107 64-65 0.07528560369387094 1107 66-67 0.19921675902393687 1107 68-69 0.38706557358387883 1107 70-71 0.2067895831527835 1107 72-73 0.3622056099653115 1107 74-75 0.31883013756116085 1107 76-77 0.2899858702110798 1107 78-79 0.3981765555312471 1107 80-81 0.5411958777073771 1107 82-83 0.4743400131760396 1107 84-85 0.6991317635288326 1107 86-87 0.5309434362235379 1107 88-89 0.557911023271739 1107 90-91 0.46560102960186 1107 92-93 0.6821233570534666 1107 94-95 0.8700329727925684 1107 96-97 1.0706898395328537 1107 98-99 0.8687593477513147 1107 100-101 1.2045067324409438 1108 1 0.10166665446335443 1108 2 0.20799049776334755 1108 3 0.14842493637414833 1108 4 4.498971553488218E-4 1108 5 0.10351434333700382 1108 6 0.17433035037689848 1108 7 0.13837022594830017 1108 8 0.11552327957473807 1108 9 -0.004085077873668297 1108 10-11 0.12772135170182963 1108 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-1.765592985156637 2316 92-93 -1.9424134155580681 2316 94-95 -2.141437212782783 2316 96-97 -2.434467379309549 2316 98-99 -3.1661330997561627 2316 100-101 -3.7823852458155116 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 21.0 4 44.0 5 109.0 6 244.0 7 554.0 8 1231.0 9 2306.0 10 3759.0 11 5704.0 12 7810.0 13 10757.0 14 13471.0 15 17690.0 16 21259.0 17 25992.0 18 23276.0 19 17742.0 20 17343.0 21 18163.0 22 19461.0 23 22183.0 24 25921.0 25 31504.0 26 38307.0 27 47000.0 28 57677.0 29 69536.0 30 84080.0 31 101765.0 32 122248.0 33 151557.0 34 192501.0 35 249408.0 36 330303.0 37 388530.0 38 199190.0 39 8486.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.23941815030974 19.776382256197646 15.254600070745521 22.729599522747094 2 16.100487567983393 20.948232700361647 38.82803182836919 24.123247903285765 3 19.69429484457753 24.44850508047451 29.974627709772626 25.882572365175328 4 13.380836685811817 17.31924332677018 36.64172325272202 32.658196734695984 5 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29.566110073592668 21.806694414674872 26-27 22.243579289832383 26.42655650373201 29.429179727458894 21.900684478976718 28-29 21.873475816103117 26.629831998676444 29.549539871039272 21.947152314181167 30-31 22.122317345282386 26.16060100489772 29.547473736116046 22.169607913703846 32-33 21.966100646052936 26.39055443834521 29.394237543868623 22.249107371733235 34-35 22.615412910741732 26.356526854657364 29.163366781244132 21.86469345335677 36-37 22.575636897342918 26.090945401862342 29.169920865756254 22.16349683503848 38-39 22.292230537992598 26.366026966128935 29.323564970975735 22.018177524902725 40-41 22.514651608942934 26.282806723378783 29.15415037426946 22.048391293408823 42-43 22.34453083470525 26.402910654192436 28.823628502683885 22.42893000841843 44-45 22.530444092598774 26.460561210797934 28.61773710424613 22.39125759235716 46-47 22.718047552135033 26.774632272369832 28.115575838049445 22.391744337445697 48-49 22.865433900385195 26.851426911501065 27.823442154350175 22.459697033763565 50-51 22.66608254318027 27.079129753406928 27.823221143092997 22.43156656031981 52-53 23.082139140886166 27.03449591675241 27.567856637176124 22.31550830518529 54-55 22.621429751623968 26.874003720945822 27.796225897808707 22.708340629621503 56-57 22.819422905055184 27.16941374014504 27.473155838499025 22.538007516300755 58-59 23.054851845515994 26.8042748128351 27.532775886094395 22.608097455554514 60-61 22.780510804217506 26.890652104024436 28.014117946399253 22.3147191453588 62-63 22.663044867999965 26.992270406622897 27.907010226116014 22.43767449926112 64-65 22.533434619430384 26.963576760213044 27.819365486296828 22.68362313405974 66-67 22.569281060075745 27.1348974400955 27.50968059098982 22.786140908838934 68-69 22.634873966371266 26.876689053451113 27.762076423316916 22.7263605568607 70-71 22.63708176541693 26.823735471930483 27.706882245234805 22.832300517417785 72-73 22.726087109723807 26.824497429026433 27.723672122017902 22.72574333923186 74-75 22.5773307736354 27.292840115207895 27.41124942122107 22.718579689935638 76-77 22.682018071117774 27.055238885927384 27.526107249696885 22.736635793257957 78-79 22.69964949495644 27.04234939800458 27.487055895154473 22.77094521188451 80-81 22.65560818667331 27.319840732789046 27.267966502226358 22.756584578311283 82-83 22.722397609885547 27.241855110807972 27.359035702366885 22.6767115769396 84-85 22.590133324851344 27.211263452622784 27.276103396607258 22.922499825918614 86-87 22.674823751065528 27.13927388597716 27.219660031711808 22.9662423312455 88-89 22.623547011486348 27.17312057225394 26.979116514203177 23.22421590205654 90-91 22.54510663561611 27.20963295199387 27.176831252668094 23.06842915972192 92-93 22.692903672278135 27.08985873538837 27.200196863508687 23.017040728824814 94-95 22.45997867133874 26.97130001242238 27.276765814996 23.291955501242885 96-97 22.427943377910232 26.93627048457923 27.302045813918895 23.33374032359165 98-99 22.368083895254752 27.01438295267134 26.975280616763577 23.642252535310334 100-101 22.1895357251422 27.01858587092006 27.124140994127178 23.667737409810563 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 399.0 1 307.0 2 293.5 3 383.0 4 322.0 5 279.5 6 467.0 7 961.5 8 1535.0 9 1835.0 10 1736.0 11 1549.5 12 1595.5 13 1823.0 14 2130.0 15 2376.0 16 2591.5 17 2902.5 18 3189.5 19 3455.5 20 3910.5 21 4603.0 22 5320.5 23 6363.5 24 7787.5 25 9429.0 26 11867.5 27 15039.5 28 18120.5 29 21714.0 30 26387.0 31 31726.0 32 37257.0 33 41099.5 34 45146.5 35 48981.0 36 53058.0 37 58440.5 38 62396.0 39 64241.5 40 66184.5 41 70810.0 42 74955.0 43 78276.5 44 81172.0 45 84227.5 46 85808.5 47 88915.0 48 98584.0 49 99107.0 50 90319.5 51 84763.5 52 80388.5 53 78368.5 54 76818.5 55 71502.5 56 64493.0 57 57820.5 58 52630.0 59 46811.0 60 39634.5 61 32989.5 62 27580.0 63 21734.5 64 16068.5 65 12572.5 66 9681.5 67 7668.0 68 5690.0 69 3842.5 70 2970.5 71 2421.0 72 2042.5 73 1815.5 74 1523.0 75 1246.5 76 930.5 77 632.5 78 410.5 79 290.0 80 217.0 81 148.0 82 98.0 83 66.0 84 42.5 85 19.5 86 8.5 87 5.5 88 4.0 89 2.5 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0208410860741152 2 0.011988995906552866 3 0.007992663937701911 4 0.0 5 0.002707192624060325 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0012031967218045889 14-15 1.9337090171859465E-4 16-17 0.0 18-19 0.0013321106562836518 20-21 0.0 22-23 2.1485655746510513E-5 24-25 0.0 26-27 0.0015254815580022466 28-29 0.002965020493018451 30-31 0.0013535963120301624 32-33 0.006767981560150812 34-35 0.0014180532792696938 36-37 5.371413936627629E-4 38-39 0.0012676536890441205 40-41 2.1485655746510513E-5 42-43 0.00416821721482304 44-45 0.0017403381154673517 46-47 1.718852459720841E-4 48-49 4.297131149302103E-4 50-51 2.1485655746510513E-5 52-53 1.503995902255736E-4 54-55 0.0016114241809882886 56-57 0.013944190579485323 58-59 0.024751475419980115 60-61 0.019337090171859462 62-63 0.012783965169173756 64-65 0.01682326844951773 66-67 0.0023634221321161566 68-69 7.305122953813575E-4 70-71 4.2971311493021026E-5 72-73 0.0 74-75 0.0015684528694952676 76-77 0.0021700512303975622 78-79 0.008852090167562332 80-81 0.015147387301289911 82-83 0.016479497957573565 84-85 0.02732975410956138 86-87 0.010334600414071558 88-89 0.0384163524747608 90-91 0.04447530739527677 92-93 0.027888381158970646 94-95 0.029521290995705447 96-97 0.047955983626211465 98-99 0.05096397543072294 100-101 0.05736670084318308 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2327134.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.5322919645332 #Duplication Level Percentage of deduplicated Percentage of total 1 77.9146627014877 44.82609122860116 2 13.047643629724432 15.013216855089754 3 4.076474476621391 7.035867593248487 4 1.6840674550418975 3.8755304204575536 5 0.8992161239114518 2.586698229004475 6 0.5129362321059989 1.770623823882594 7 0.3362981181902058 1.3543601065989408 8 0.23464676238683255 1.0799812833737357 9 0.18203563408211562 0.9425634523165085 >10 0.975538053194845 10.413772840968347 >50 0.08583569914391133 3.4086317557203447 >100 0.046447723918605834 4.895915596975666 >500 0.0030730892467170537 1.1973367464989588 >1k 9.743941513978509E-4 0.9464937062918918 >5k 1.499067925227463E-4 0.6529163609715948 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7944 0.34136409850055904 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7197 0.3092645288152724 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4298 0.1846906967970044 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3837 0.1648809221987217 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.297131149302103E-5 0.0 14-15 0.0 0.0 0.0 4.297131149302103E-5 0.0 16-17 0.0 0.0 0.0 4.297131149302103E-5 0.0 18-19 0.0 0.0 0.0 4.297131149302103E-5 0.0 20-21 0.0 4.297131149302103E-5 0.0 4.297131149302103E-5 0.0 22-23 0.0 4.297131149302103E-5 0.0 8.594262298604206E-5 0.0 24-25 0.0 4.297131149302103E-5 0.0 1.289139344790631E-4 0.0 26-27 0.0 4.297131149302103E-5 0.0 3.437704919441682E-4 0.0 28-29 0.0 4.297131149302103E-5 0.0 0.0011387397545650573 0.0 30-31 0.0 4.297131149302103E-5 0.0 0.0029865061487649616 0.0 32-33 0.0 6.445696723953155E-5 0.0 0.005306956969388097 0.0 34-35 0.0 8.594262298604206E-5 0.0 0.00917437500375999 0.0 36-37 0.0 8.594262298604206E-5 0.0 0.013901219267992303 4.297131149302103E-5 38-39 0.0 8.594262298604206E-5 0.0 0.019079262302901336 4.297131149302103E-5 40-41 0.0 8.594262298604206E-5 0.0 0.02404244878034527 4.297131149302103E-5 42-43 0.0 1.0742827873255257E-4 0.0 0.030767459029003057 4.297131149302103E-5 44-45 0.0 1.289139344790631E-4 0.0 0.0382014959172957 4.297131149302103E-5 46-47 0.0 1.289139344790631E-4 0.0 0.049588893462946274 4.297131149302103E-5 48-49 0.0 1.289139344790631E-4 0.0 0.062437315599359555 4.297131149302103E-5 50-51 0.0 1.289139344790631E-4 0.0 0.07511385248980076 4.297131149302103E-5 52-53 0.0 1.289139344790631E-4 0.0 0.0946228279076323 4.297131149302103E-5 54-55 0.0 1.289139344790631E-4 0.0 0.12601337095328416 4.297131149302103E-5 56-57 0.0 1.289139344790631E-4 0.0 0.17066056359453302 4.297131149302103E-5 58-59 0.0 1.289139344790631E-4 0.0 0.21689769476102366 4.297131149302103E-5 60-61 0.0 1.289139344790631E-4 0.0 0.26416613740334677 4.297131149302103E-5 62-63 0.0 1.289139344790631E-4 0.0 0.31704233619550914 4.297131149302103E-5 64-65 0.0 1.289139344790631E-4 0.0 0.3794366804833757 4.297131149302103E-5 66-67 0.0 1.289139344790631E-4 0.0 0.46447690592806434 4.297131149302103E-5 68-69 0.0 1.289139344790631E-4 0.0 0.5718192420376309 4.297131149302103E-5 70-71 0.0 1.289139344790631E-4 0.0 0.675272674457079 4.297131149302103E-5 72-73 0.0 1.289139344790631E-4 0.0 0.7851288322889872 4.297131149302103E-5 74-75 0.0 1.289139344790631E-4 0.0 0.9024619983206812 4.297131149302103E-5 76-77 0.0 1.289139344790631E-4 0.0 1.0327939860790138 4.297131149302103E-5 78-79 0.0 1.289139344790631E-4 0.0 1.1653819676907302 4.297131149302103E-5 80-81 0.0 1.289139344790631E-4 0.0 1.3150940169324157 4.297131149302103E-5 82-83 0.0 1.289139344790631E-4 0.0 1.479416312081728 4.297131149302103E-5 84-85 0.0 1.289139344790631E-4 0.0 1.672336015029646 4.297131149302103E-5 86-87 0.0 1.289139344790631E-4 0.0 1.8896419372498534 4.297131149302103E-5 88-89 0.0 1.289139344790631E-4 0.0 2.1324728184969155 4.297131149302103E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10605 0.0 28.220919 1 GTATCAA 14510 0.0 26.19171 1 CGTATAG 100 0.0016530966 23.752607 1 GTACATG 10275 0.0 23.34806 1 TACATGG 10505 0.0 22.247557 2 GAGTACT 9640 0.0 22.021679 12-13 ATCAACG 17580 0.0 21.478579 3 TATCAAC 17735 0.0 21.427528 2 TCAACGC 17605 0.0 21.39182 4 CAACGCA 17790 0.0 21.222757 5 AACGCAG 18240 0.0 20.672688 6 ACATGGG 10560 0.0 20.64458 3 GTACTTT 10200 0.0 20.626402 14-15 AGTACTT 9940 0.0 19.469784 12-13 TATACCG 175 9.9569355E-5 18.996368 5 AGAGTAC 16185 0.0 18.822844 10-11 ACGCAGA 20040 0.0 18.792162 7 CGCAGAG 20250 0.0 18.59728 8 TACCTGG 1935 0.0 18.166195 2 TAGCCCT 1570 0.0 18.149397 4 >>END_MODULE