FastQCFastQC Report
Thu 26 May 2016
SRR936451_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936451_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721188
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT59180.8205904701686662No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31060.4306782697438116No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28790.39920242710638554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24290.3368053822304309No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT21840.30283365779796667No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC20800.28841300742663495No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT17530.24307115481677455No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC13970.19370815931490817No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC13110.18178339073861463No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13060.1810900902399929No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT12950.17956482914302513No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG10880.15086218850008595No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10810.14989156780201557No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA10620.14725702590725304No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC9550.13242039523674826No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA8900.12340748875466591No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA8800.12202088775742248No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG902.4109771E-531.66815
GTGTACG650.00584531429.2280391
GCACCGT2104.3655746E-1127.1440876
TACCTGG5400.026.3882542
GTACCTG6300.024.8786281
GTACATG38850.024.3284871
TACATGG39200.023.2647462
ACATGGG40000.022.326013
GTACACG1100.002877896721.5888921
GTAAGCG804.9647322E-520.79517694-95
AGTACTT29200.020.73936312-13
AAGGTGT4900.020.3580636
GAGTACT27850.020.03921712-13
AGGTAAG3102.582965E-1019.9188752
ACTAGGT1456.141117E-419.6560633
ATAGCCC1951.0313683E-519.4880623
GGTAAGG3203.947207E-1019.2977493
GTACTTT29350.019.01902414-15
ACAGCGC1250.0060298518.999549
CATGGGC7050.018.8661044
Produced by FastQC (version 0.11.5)