##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936451_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 721188 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.417824478499366 31.0 31.0 34.0 30.0 34.0 2 31.473296837995086 31.0 31.0 34.0 28.0 34.0 3 31.49389202260714 33.0 31.0 34.0 28.0 34.0 4 35.109992124106334 37.0 35.0 37.0 32.0 37.0 5 35.12641918612068 37.0 35.0 37.0 32.0 37.0 6 35.094334625645466 37.0 35.0 37.0 32.0 37.0 7 35.001275672917465 37.0 35.0 37.0 32.0 37.0 8 34.961778343510986 37.0 35.0 37.0 32.0 37.0 9 36.46478449447301 39.0 37.0 39.0 32.0 39.0 10-11 36.33583684143386 39.0 37.0 39.0 32.0 39.0 12-13 36.26426465775914 39.0 36.0 39.0 32.0 39.0 14-15 37.3588558045891 40.0 37.0 41.0 32.0 41.0 16-17 37.28072499819742 40.0 37.0 41.0 32.0 41.0 18-19 37.27760861245611 40.0 37.0 41.0 31.5 41.0 20-21 37.14453651475066 39.5 36.5 41.0 31.0 41.0 22-23 37.24086021952667 40.0 37.0 41.0 31.0 41.0 24-25 37.28780151638685 40.0 37.0 41.0 31.0 41.0 26-27 37.187756867834736 39.5 36.5 41.0 31.0 41.0 28-29 37.060548705746626 39.0 36.0 41.0 31.0 41.0 30-31 36.86367424305452 39.0 36.0 41.0 30.0 41.0 32-33 36.666610509326276 39.0 35.5 41.0 30.0 41.0 34-35 36.45769133707161 39.0 35.0 41.0 30.0 41.0 36-37 36.23093839609089 39.0 35.0 40.0 30.0 41.0 38-39 36.02770983432892 39.0 35.0 40.0 29.5 41.0 40-41 35.84538358929988 38.0 35.0 40.0 29.0 41.0 42-43 35.58886309811034 38.0 35.0 40.0 27.5 41.0 44-45 35.285364565134195 38.0 34.5 40.0 26.5 41.0 46-47 35.05484630914547 38.0 34.0 40.0 25.5 41.0 48-49 34.86146053456242 38.0 34.0 40.0 24.0 41.0 50-51 33.85696933393234 37.5 33.0 39.5 23.5 40.5 52-53 33.944601823657635 37.5 33.5 39.5 23.0 40.5 54-55 34.37390874501517 38.0 34.0 40.0 23.0 41.0 56-57 34.24530080922034 38.0 34.0 40.0 23.0 41.0 58-59 34.002638008397255 38.0 33.5 40.0 22.5 41.0 60-61 33.66561978291375 37.0 33.0 40.0 22.0 41.0 62-63 33.32185505027815 37.0 33.0 40.0 20.5 41.0 64-65 32.96244807179265 36.0 32.0 39.5 19.5 41.0 66-67 32.45484256532277 36.0 32.0 39.0 18.0 41.0 68-69 32.06929191833475 35.0 31.0 39.0 15.0 40.0 70-71 31.646864617825035 35.0 31.0 38.0 12.0 40.0 72-73 31.247437561357096 35.0 31.0 37.0 9.0 39.5 74-75 30.847358109119952 35.0 30.5 37.0 7.5 39.0 76-77 30.37779122780745 34.5 30.0 36.0 4.5 39.0 78-79 29.871118210508218 34.0 29.0 36.0 2.0 38.5 80-81 29.392579327443052 34.0 29.0 35.0 2.0 37.0 82-83 28.983638108232526 34.0 29.0 35.0 2.0 37.0 84-85 28.64127730910664 34.0 29.0 35.0 2.0 36.0 86-87 28.32332623393623 34.0 28.5 35.0 2.0 36.0 88-89 27.898322628773634 34.0 27.0 35.0 2.0 36.0 90-91 27.45087688647066 33.5 26.0 35.0 2.0 35.0 92-93 26.985546071204734 33.0 25.5 35.0 2.0 35.0 94-95 26.474055308740578 33.0 25.0 35.0 2.0 35.0 96-97 25.87844570347815 33.0 23.5 35.0 2.0 35.0 98-99 25.149180934790927 32.5 18.5 35.0 2.0 35.0 100-101 22.411249909870936 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.49674947771357125 1101 2 0.4414716688797071 1101 3 0.5420482287047683 1101 4 0.5625678401680716 1101 5 0.4610781652927116 1101 6 0.4800699054109714 1101 7 0.44999821830522535 1101 8 0.43886112660668175 1101 9 0.6557368001417885 1101 10-11 0.7307579905153219 1101 12-13 0.7920535829577346 1101 14-15 0.790363185412474 1101 16-17 0.8787890707075192 1101 18-19 0.742245910785293 1101 20-21 0.7860475010882979 1101 22-23 0.6374296647409636 1101 24-25 0.676958449481809 1101 26-27 0.6718444439322013 1101 28-29 0.7242119454763909 1101 30-31 0.7208909894037916 1101 32-33 0.4769685588588928 1101 34-35 0.4742380579002372 1101 36-37 0.5739590162327275 1101 38-39 0.5286033090268774 1101 40-41 0.5675783085321342 1101 42-43 0.5767551287978705 1101 44-45 0.3803388550921838 1101 46-47 0.45912448893049884 1101 48-49 0.7409766915696352 1101 50-51 0.6434244714889701 1101 52-53 0.7710972911844891 1101 54-55 0.6454808920717525 1101 56-57 0.7113568494242415 1101 58-59 0.7437531323380142 1101 60-61 0.8305196370772592 1101 62-63 0.711922525750019 1101 64-65 0.5356700246870361 1101 66-67 0.7582502163837361 1101 68-69 1.012683786726214 1101 70-71 1.0305381867587897 1101 72-73 0.9780424748750427 1101 74-75 0.8842169901129715 1101 76-77 0.8369600680979126 1101 78-79 0.7330472679716529 1101 80-81 0.6731799935471763 1101 82-83 0.5925533486669501 1101 84-85 0.6273546616244658 1101 86-87 0.5666132079060127 1101 88-89 0.5681688092637955 1101 90-91 0.35556039374996473 1101 92-93 0.20098329652923397 1101 94-95 0.06062113984610562 1101 96-97 0.2667278013532375 1101 98-99 0.12167986266506503 1101 100-101 0.3853101433796873 1102 1 0.3867475681997874 1102 2 0.574050679844337 1102 3 0.44048296855864066 1102 4 0.4819724429466845 1102 5 0.37444731296152156 1102 6 0.5255526683769176 1102 7 0.3623198391809268 1102 8 0.38147289307701016 1102 9 0.7284707213785921 1102 10-11 0.6874989993725293 1102 12-13 0.6539876189199845 1102 14-15 0.7764465407142396 1102 16-17 0.5639500612735944 1102 18-19 0.6229152146940748 1102 20-21 0.4338759509883019 1102 22-23 0.3941931702990473 1102 24-25 0.40299888324293676 1102 26-27 0.22422426245881866 1102 28-29 0.07836065408071846 1102 30-31 0.29228979715595216 1102 32-33 0.27893883692894406 1102 34-35 0.24133419766737063 1102 36-37 0.17877392868499697 1102 38-39 0.35148469575134555 1102 40-41 0.11996933675505517 1102 42-43 0.32026877331752956 1102 44-45 0.46731102334894814 1102 46-47 0.40337557685084846 1102 48-49 0.48431175403083415 1102 50-51 0.5240578270613128 1102 52-53 0.5884952037071116 1102 54-55 0.7843995661115599 1102 56-57 0.618046177716046 1102 58-59 0.3261486521702892 1102 60-61 0.34333231995959324 1102 62-63 0.5372429877750378 1102 64-65 0.48774857296179164 1102 66-67 0.5573678200135177 1102 68-69 0.4879930777090493 1102 70-71 0.6055414916864947 1102 72-73 0.27376160781663117 1102 74-75 0.3957676158072978 1102 76-77 0.4782370303165955 1102 78-79 0.3895566613106176 1102 80-81 0.4839228253329253 1102 82-83 0.4459912079087225 1102 84-85 0.10458622225384673 1102 86-87 0.5087448901412728 1102 88-89 0.4350354981436979 1102 90-91 0.751762857873139 1102 92-93 0.5342502736142976 1102 94-95 0.43714981386272456 1102 96-97 0.502216849479943 1102 98-99 0.458203449798809 1102 100-101 0.5348838270327825 1103 1 0.2602366191598193 1103 2 0.2785305253892041 1103 3 0.2804436157552175 1103 4 0.25859447589773055 1103 5 0.1937893082225557 1103 6 0.22997523988863122 1103 7 0.16233923845022247 1103 8 0.34091271608177465 1103 9 0.16529607714733885 1103 10-11 0.4572841755594652 1103 12-13 0.13539572873832384 1103 14-15 0.40876064259735756 1103 16-17 0.43183410906969755 1103 18-19 0.4428220982014679 1103 20-21 0.2734610837578231 1103 22-23 0.22273688562862048 1103 24-25 0.2773793768157802 1103 26-27 0.17396029383020561 1103 28-29 0.39373180367217486 1103 30-31 0.19292951115529888 1103 32-33 0.3586702956154326 1103 34-35 0.6220504002142206 1103 36-37 0.656515114719376 1103 38-39 0.48163670288288785 1103 40-41 0.3317710207963884 1103 42-43 0.586195804351739 1103 44-45 0.6987798713556757 1103 46-47 0.766303111342296 1103 48-49 0.6090444367188397 1103 50-51 0.7798644440596831 1103 52-53 0.9622530731130752 1103 54-55 1.0687914683022939 1103 56-57 1.0225204806125348 1103 58-59 0.8436504881725639 1103 60-61 0.9259976871831341 1103 62-63 0.6794641117317539 1103 64-65 0.713428586633654 1103 66-67 0.7271973373874019 1103 68-69 0.5821626735114194 1103 70-71 0.7395359398003762 1103 72-73 0.6395302114849422 1103 74-75 0.5593995285290596 1103 76-77 0.6567514157985563 1103 78-79 0.3557432730434833 1103 80-81 0.49916233702560575 1103 82-83 0.42435363244787894 1103 84-85 0.6511172172054209 1103 86-87 0.3584435086361637 1103 88-89 0.43196948982048866 1103 90-91 0.27247942992987717 1103 92-93 0.33216900915144976 1103 94-95 0.39043452585286076 1103 96-97 0.2831025646894325 1103 98-99 0.3476354669071178 1103 100-101 0.5192974379296267 1104 1 0.3152352386190742 1104 2 0.3966623351719285 1104 3 0.20060057261862596 1104 4 0.44615155594784994 1104 5 0.4147873329873377 1104 6 0.43208428136857435 1104 7 0.5845701922976829 1104 8 0.5613882592714603 1104 9 0.7822862859275759 1104 10-11 0.8078661265821268 1104 12-13 0.5905217827280893 1104 14-15 0.37134214759915807 1104 16-17 0.4048286081457988 1104 18-19 0.5427243983947321 1104 20-21 0.3187146784735404 1104 22-23 0.514454172122683 1104 24-25 0.6662564662111023 1104 26-27 0.45481378659829375 1104 28-29 0.37188633927951287 1104 30-31 0.5655464854347585 1104 32-33 0.4478909272659166 1104 34-35 0.5037285005901495 1104 36-37 0.12063910851755821 1104 38-39 0.42345912286462095 1104 40-41 0.1156643580943495 1104 42-43 0.04974421885378888 1104 44-45 0.01830974413353914 1104 46-47 -0.19395377543212788 1104 48-49 0.06066444818794281 1104 50-51 0.15534444745794218 1104 52-53 0.2059726746677768 1104 54-55 0.07660320502255757 1104 56-57 0.15995809079030465 1104 58-59 0.36873491354902654 1104 60-61 0.23757670721159485 1104 62-63 0.44352332631026314 1104 64-65 0.47660160024791054 1104 66-67 0.5760525505216592 1104 68-69 0.34230433823117323 1104 70-71 0.46861118989064465 1104 72-73 0.32225805380594963 1104 74-75 0.49418915535827423 1104 76-77 0.1532915682952094 1104 78-79 0.22913600502742426 1104 80-81 0.4558871633960564 1104 82-83 0.2700776729720573 1104 84-85 0.11743786372327136 1104 86-87 0.33297412478211896 1104 88-89 0.4270722870084356 1104 90-91 0.2210155257606452 1104 92-93 -0.019915076399598775 1104 94-95 0.25852651234480106 1104 96-97 0.4703787577952241 1104 98-99 0.5505972577870502 1104 100-101 0.6127308427678919 1105 1 0.6851097156065222 1105 2 0.4909353477242284 1105 3 0.6387177274303433 1105 4 0.6107645266591533 1105 5 0.5202423539078396 1105 6 0.7039409759292425 1105 7 0.8461653805821996 1105 8 0.7913568785183216 1105 9 0.961799884253935 1105 10-11 0.826548134950329 1105 12-13 0.7062618454895926 1105 14-15 0.8088970639171578 1105 16-17 0.7073861395265482 1105 18-19 0.776581615967963 1105 20-21 0.7337434441639132 1105 22-23 0.44155668258294156 1105 24-25 0.6389656712320217 1105 26-27 0.5451563598200551 1105 28-29 0.4799564229615214 1105 30-31 0.477047531459867 1105 32-33 0.3918841281027383 1105 34-35 0.46832578092487154 1105 36-37 0.67459569847572 1105 38-39 0.605697616588472 1105 40-41 0.620235487801466 1105 42-43 0.792280829732448 1105 44-45 0.5797950997820678 1105 46-47 0.8936508270783321 1105 48-49 0.8521916448406657 1105 50-51 0.5272089872068904 1105 52-53 0.7328241391029167 1105 54-55 0.6198511129723485 1105 56-57 0.6935642623384268 1105 58-59 0.7901130516243384 1105 60-61 0.861530682106995 1105 62-63 0.871253451833276 1105 64-65 1.0421690688671532 1105 66-67 0.8929746216513692 1105 68-69 1.1743676227499975 1105 70-71 0.9488570057902308 1105 72-73 0.6812434094544848 1105 74-75 0.9642440716762657 1105 76-77 0.9931346645295172 1105 78-79 1.155257866952109 1105 80-81 1.2560309918479398 1105 82-83 1.238890434478332 1105 84-85 1.3148228009617675 1105 86-87 1.0321713005143422 1105 88-89 0.9975246928661754 1105 90-91 0.8183455466811438 1105 92-93 0.9195357604204837 1105 94-95 1.1566305918427062 1105 96-97 1.5083468540295364 1105 98-99 1.5975847054857972 1105 100-101 1.5505315749854667 1106 1 0.15139028188708537 1106 2 0.016393841932721642 1106 3 0.14479237350498764 1106 4 0.04354766694228829 1106 5 -0.05650957659409528 1106 6 0.13251723075549648 1106 7 0.45004086320768266 1106 8 0.19433306899450997 1106 9 0.4614958301998797 1106 10-11 0.5315095249117192 1106 12-13 0.25288588023862957 1106 14-15 0.4214268258219107 1106 16-17 0.35184077273118675 1106 18-19 0.20429960181094486 1106 20-21 0.11123620478167595 1106 22-23 0.2659357044619597 1106 24-25 0.21968317444952845 1106 26-27 0.18075722854592868 1106 28-29 0.3177797820348829 1106 30-31 0.2988026794650125 1106 32-33 -0.11339981655621045 1106 34-35 -0.03172730787821365 1106 36-37 0.0694484977035188 1106 38-39 0.3171004479913009 1106 40-41 0.12026444533042024 1106 42-43 0.2862657074673294 1106 44-45 0.3053292053161769 1106 46-47 0.16335803428553675 1106 48-49 0.10779010043911796 1106 50-51 -0.4225521131792078 1106 52-53 -0.09079555201677181 1106 54-55 -0.05754110066987295 1106 56-57 -0.2527782408790813 1106 58-59 -0.1697526176163251 1106 60-61 0.29846761904393304 1106 62-63 0.13247288555270842 1106 64-65 0.17164220271529018 1106 66-67 -0.030861446559704575 1106 68-69 0.15242183767921347 1106 70-71 -0.12367999549677933 1106 72-73 -0.03518837947730802 1106 74-75 0.18375259805979383 1106 76-77 -1.9545130059483995E-4 1106 78-79 -0.05090928234004011 1106 80-81 0.29301216945411923 1106 82-83 0.1684415927794909 1106 84-85 0.4060068421458034 1106 86-87 0.22687931209735268 1106 88-89 0.24900233121881143 1106 90-91 0.3145513060119036 1106 92-93 0.4436847308194558 1106 94-95 0.2599084232455411 1106 96-97 0.3318531281608088 1106 98-99 0.5137494416505355 1106 100-101 0.3539863690652574 1107 1 0.3272972156065208 1107 2 0.28520618105756057 1107 3 0.38804064409701056 1107 4 0.44362910999248584 1107 5 0.4443048539078376 1107 6 0.49357639259590513 1107 7 0.5989257972488673 1107 8 0.5455235451849845 1107 9 0.6522686342539359 1107 10-11 0.6500116766169981 1107 12-13 0.479855595489596 1107 14-15 0.24220435558382292 1107 16-17 0.37097988952655214 1107 18-19 0.41962849096795907 1107 20-21 0.4736653191639135 1107 22-23 0.42960355758293645 1107 24-25 0.3570906712320223 1107 26-27 0.4135938598200539 1107 28-29 0.5218574646281908 1107 30-31 0.4313964897931939 1107 32-33 0.2134205864360723 1107 34-35 0.24392473925820468 1107 36-37 0.3776425734757183 1107 38-39 0.38087990825513884 1107 40-41 0.17901152946813426 1107 42-43 0.2099631213991131 1107 44-45 0.32805030811540803 1107 46-47 -0.0536147979216679 1107 48-49 0.07536872817399143 1107 50-51 0.27939648720689547 1107 52-53 0.2715480974362521 1107 54-55 0.5226375713056797 1107 56-57 0.38064759567176054 1107 58-59 0.42071200995766844 1107 60-61 0.5555410987736593 1107 62-63 0.5802638684999479 1107 64-65 0.4739919855338286 1107 66-67 0.6389121216513658 1107 68-69 0.7018155394166712 1107 70-71 0.5631538807902245 1107 72-73 0.5388996594544828 1107 74-75 0.7088013633429355 1107 76-77 0.5609471645295194 1107 78-79 0.526117241952111 1107 80-81 0.8087653668479398 1107 82-83 0.8434737678116697 1107 84-85 0.7840936342950968 1107 86-87 0.6645150505143391 1107 88-89 0.6809621928661755 1107 90-91 0.7550903383478129 1107 92-93 0.9583118020871524 1107 94-95 0.8575420501760398 1107 96-97 0.8174874790295341 1107 98-99 0.984615955485797 1107 100-101 0.5996201166521331 1108 1 0.2646924279761187 1108 2 0.24796776203123372 1108 3 0.35862830865239914 1108 4 0.3622809201017745 1108 5 0.2778671676187159 1108 6 0.3381976823375865 1108 7 0.28783266821260867 1108 8 0.04111470266138895 1108 9 0.14446195578895527 1108 10-11 0.07820983390710978 1108 12-13 0.02812194235994525 1108 14-15 0.46354590892460124 1108 16-17 0.3237032036100089 1108 18-19 0.15884409733904903 1108 20-21 0.3483829747153351 1108 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-1.731117133047892 2316 80-81 -1.5785940081520629 2316 82-83 -1.582859565521666 2316 84-85 -1.3096771990382372 2316 86-87 -1.4017036994856618 2316 88-89 -1.7373503071338234 2316 90-91 -1.942779453318856 2316 92-93 -2.864214239579514 2316 94-95 -2.703494408157294 2316 96-97 -2.6447781459704665 2316 98-99 -4.010415294514203 2316 100-101 -5.0270934250145345 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1822.0 3 732.0 4 654.0 5 721.0 6 727.0 7 919.0 8 1151.0 9 1439.0 10 1848.0 11 2388.0 12 3133.0 13 3819.0 14 4196.0 15 5188.0 16 6607.0 17 8349.0 18 6820.0 19 5008.0 20 5021.0 21 5279.0 22 5959.0 23 6751.0 24 7697.0 25 8661.0 26 9991.0 27 12003.0 28 14034.0 29 16847.0 30 20108.0 31 25037.0 32 31309.0 33 39879.0 34 52811.0 35 71189.0 36 97851.0 37 125944.0 38 95655.0 39 13637.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.68865331436163 18.407403401037897 14.037948948374233 21.86599433622624 2 15.55134271560834 21.072674724994314 39.29821544469654 24.07776711470081 3 20.85005644483523 24.916026172651854 29.440904892532917 24.79301248998 4 12.69082209354738 16.41232966956763 37.66942893797025 33.22741929891474 5 13.506679058412285 38.24499590794967 33.399453468532826 14.848871565105215 6 31.24849120528765 36.12769051047901 17.289885595207373 15.333932689025964 7 28.501101071117546 33.498494000965174 20.289107439025067 17.711297488892217 8 26.772691083906867 32.909180183254136 19.538369354449447 20.77975937838955 9 26.459817564418405 17.75195863017955 20.849285349668197 34.93893845573385 10-11 25.42617764586661 26.43019301371773 26.47333619567306 21.6702931447426 12-13 27.383682825249416 24.096597785491607 26.831893637016208 21.68782575224277 14-15 23.469389878114328 25.56804272066855 26.618305390363577 24.344262010853544 16-17 21.990286245737316 29.001930128381627 26.967631684621324 22.040151941259737 18-19 21.770637054564947 27.986113085698154 29.05024019840034 21.193009661336554 20-21 22.99437230998915 26.22016533420653 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46-47 23.048241311246276 27.491467068047836 27.622225627152297 21.83806599355359 48-49 22.836824951347065 27.48968876680494 27.4404638512326 22.2330224306154 50-51 21.99870070171814 27.932833073450563 28.0812054492875 21.987260775543795 52-53 22.73272176003317 28.25407632717669 26.98803500819183 22.025166904598308 54-55 21.809165640496555 27.529301316825862 27.562582509623812 23.09895053305377 56-57 22.7104441766723 27.713654206942262 27.08045320358248 22.49544841280296 58-59 23.492059970227057 27.1286719560908 26.931271923225463 22.447996150456685 60-61 22.467416671404465 27.416033535279023 28.386149364002822 21.730400429313697 62-63 22.17927563160376 27.75187886519316 28.026636900635072 22.042208602568014 64-65 21.878249873124954 28.407669631244264 27.48231375713759 22.231766738493196 66-67 22.10143972794814 27.91360800178631 26.96761201543883 23.017340254826717 68-69 21.942386408802758 27.74113352926773 27.463602191672116 22.852877870257394 70-71 22.60942562387695 27.848743227023913 26.97833341427372 22.56349773482541 72-73 22.344153173937798 27.30881168983817 27.897894212012847 22.44914092421119 74-75 21.786396723505934 28.92581071380268 27.034682702344664 22.253109860346722 76-77 22.29911969767479 27.742596354370754 27.023401907633794 22.934882040320662 78-79 22.379868733262104 28.02253458587148 27.134610848238445 22.46298583262797 80-81 22.668866188869742 28.149743323531716 26.83287540798615 22.348515079612387 82-83 22.40263839576214 27.92209684200011 27.367112457873215 22.308152304364537 84-85 22.13077864575207 28.28512088651265 27.194871208102327 22.389229259632952 86-87 22.37520977517649 28.302034645844028 26.820501796091595 22.502253782887895 88-89 22.011449415566627 28.1890713906775 26.4202025665831 23.37927662717277 90-91 22.160139287745732 27.741915345236606 27.00198387924696 23.0959614877707 92-93 22.644046685266982 28.2169981365008 26.986617065824454 22.152338112407772 94-95 22.2181849952917 28.014941746051335 26.98216670572915 22.784706552927815 96-97 21.52495194348054 28.037754533123632 27.50941020157387 22.92788332182196 98-99 22.090878378144012 28.37859493072884 26.314924152398632 23.215602538728515 100-101 21.849028116417873 27.780175434457703 26.745220375486912 23.62557607363751 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 261.0 1 191.0 2 151.5 3 197.0 4 184.5 5 197.0 6 288.0 7 434.0 8 648.0 9 786.0 10 751.5 11 746.5 12 799.5 13 847.5 14 914.5 15 973.5 16 1069.0 17 1133.5 18 1170.5 19 1300.0 20 1469.5 21 1686.0 22 1970.5 23 2314.5 24 2723.0 25 3278.0 26 4129.0 27 5176.0 28 6080.5 29 7107.5 30 8514.5 31 10095.0 32 11925.5 33 12939.5 34 13791.0 35 14819.0 36 15967.5 37 17438.5 38 18185.5 39 18703.5 40 19332.5 41 20396.5 42 21313.5 43 22134.5 44 23233.5 45 24516.0 46 25680.5 47 29701.0 48 39751.5 49 39228.5 50 29435.5 51 25242.5 52 23354.0 53 22832.5 54 22333.0 55 20784.0 56 18899.0 57 17089.0 58 15708.5 59 13988.0 60 11695.0 61 9615.0 62 8100.0 63 6392.5 64 4603.0 65 3553.0 66 2679.0 67 2042.5 68 1496.0 69 1044.0 70 827.5 71 661.5 72 533.0 73 472.0 74 365.0 75 279.0 76 215.5 77 134.5 78 82.0 79 56.5 80 47.5 81 33.0 82 21.5 83 17.5 84 9.0 85 5.5 86 4.5 87 4.0 88 2.5 89 2.0 90 2.5 91 2.0 92 1.5 93 0.5 94 2.0 95 2.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015807251368575186 2 0.015529931169126496 3 0.018025812964164684 4 0.022601596255068027 5 0.038547507723367555 6 0.0574052812858783 7 0.009983527180152748 8 0.030782542138804308 9 0.03827018752391887 10-11 0.046104483158344284 12-13 0.03612095597819154 14-15 0.03126785248783951 16-17 0.03487301508067245 18-19 0.030713212088942135 20-21 0.06461560647154417 22-23 0.017263182415680794 24-25 0.004159802991730311 26-27 0.002426551745176015 28-29 6.933004986217186E-5 30-31 0.0034665024931085932 32-33 0.0031198522437977335 34-35 6.933004986217186E-5 36-37 5.546403988973749E-4 38-39 6.933004986217186E-5 40-41 0.0021492315457273276 42-43 0.005823724188422436 44-45 0.0020799014958651557 46-47 0.001594591146829953 48-49 0.0011786108476569215 50-51 0.00409047294186814 52-53 0.005893054238284608 54-55 0.008319605983460623 56-57 0.001525261096967781 58-59 0.008804916332495827 60-61 0.005130423689800718 62-63 0.0011092807977947498 64-65 8.319605983460623E-4 66-67 0.020660354858927216 68-69 0.051096246748420664 70-71 0.06835942916410145 72-73 0.021007005108238073 74-75 0.040974059468543565 76-77 0.03362507418315335 78-79 0.07182593165721005 80-81 0.1010138826491844 82-83 0.061981064576781646 84-85 0.022254946005757167 86-87 0.02468149775093318 88-89 0.02641474899748748 90-91 0.05241351769580193 92-93 0.032238473185909916 94-95 0.016777872066645588 96-97 0.02246293615534368 98-99 0.060109153230503004 100-101 0.08000687754094632 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 721188.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.94649900219136 #Duplication Level Percentage of deduplicated Percentage of total 1 85.71697265550992 55.67017279041939 2 8.842413435132134 11.485675906835452 3 2.254931130808508 4.39349647311195 4 0.9770022085040652 2.5381149183899208 5 0.5252279752132161 1.7055859084054061 6 0.3189781613407936 1.2429908902344413 7 0.23352659218810368 1.0616714210570877 8 0.1595658428181445 0.8290594281097957 9 0.12319919986543958 0.7201221040018392 >10 0.7573483387278999 9.260931946726092 >50 0.05626175762049315 2.4389733899992447 >100 0.029419007523317723 3.662473195013776 >500 0.0021473728116638974 0.9617461311887487 >1k 0.0027915846551630663 3.2036354958724984 >5k 2.1473728116638973E-4 0.825350000634428 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5918 0.8205904701686662 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3106 0.4306782697438116 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2879 0.39920242710638554 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2429 0.3368053822304309 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2184 0.30283365779796667 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2080 0.28841300742663495 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1753 0.24307115481677455 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1397 0.19370815931490817 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1311 0.18178339073861463 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1306 0.1810900902399929 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1295 0.17956482914302513 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1088 0.15086218850008595 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1081 0.14989156780201557 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1062 0.14725702590725304 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 955 0.13242039523674826 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 890 0.12340748875466591 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 880 0.12202088775742248 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 6.933004986217186E-5 0.0 22-23 0.0 0.0 0.0 3.466502493108593E-4 0.0 24-25 0.0 0.0 0.0 5.546403988973749E-4 0.0 26-27 0.0 0.0 0.0 6.933004986217186E-4 6.933004986217186E-5 28-29 0.0 0.0 0.0 0.0020105714460029837 1.3866009972434372E-4 30-31 0.0 0.0 0.0 0.004506453241041171 1.3866009972434372E-4 32-33 0.0 0.0 0.0 0.008042285784011937 1.3866009972434372E-4 34-35 0.0 0.0 0.0 0.011647448376844872 1.3866009972434372E-4 36-37 0.0 0.0 0.0 0.01566859126885084 1.3866009972434372E-4 38-39 0.0 0.0 0.0 0.0231562366539654 1.3866009972434372E-4 40-41 0.0 0.0 0.0 0.03833951757378104 1.3866009972434372E-4 42-43 0.0 0.0 0.0 0.062397044875954676 1.3866009972434372E-4 44-45 0.0 0.0 0.0 0.08680122242743918 1.3866009972434372E-4 46-47 0.0 0.0 0.0 0.14198794211772797 1.3866009972434372E-4 48-49 0.0 0.0 0.0 0.19613471106008418 1.3866009972434372E-4 50-51 0.0 0.0 0.0 0.23385025818510569 1.3866009972434372E-4 52-53 0.0 0.0 0.0 0.27510163785309794 1.3866009972434372E-4 54-55 0.0 0.0 0.0 0.322315401809237 1.3866009972434372E-4 56-57 0.0 0.0 0.0 0.42284397410938623 1.3866009972434372E-4 58-59 0.0 0.0 0.0 0.5224019257114649 1.3866009972434372E-4 60-61 0.0 0.0 0.0 0.6278529315518284 1.3866009972434372E-4 62-63 0.0 0.0 0.0 0.7351758487384704 1.3866009972434372E-4 64-65 0.0 0.0 0.0 0.8508183719085731 1.3866009972434372E-4 66-67 0.0 0.0 0.0 1.0252527773617974 1.3866009972434372E-4 68-69 0.0 0.0 0.0 1.2426718137295685 1.3866009972434372E-4 70-71 0.0 0.0 0.0 1.3991497362684904 1.3866009972434372E-4 72-73 0.0 0.0 0.0 1.5385031364914559 1.3866009972434372E-4 74-75 0.0 0.0 0.0 1.7080844384543281 1.3866009972434372E-4 76-77 0.0 0.0 0.0 1.912192105248562 1.3866009972434372E-4 78-79 0.0 0.0 0.0 2.11546781144445 1.3866009972434372E-4 80-81 0.0 0.0 0.0 2.41490429679917 1.3866009972434372E-4 82-83 0.0 0.0 0.0 2.7732713245367364 1.3866009972434372E-4 84-85 0.0 0.0 0.0 3.219202205250226 1.3866009972434372E-4 86-87 0.0 0.0 0.0 3.646483302550791 1.3866009972434372E-4 88-89 0.0 0.0 0.0 4.166597336616805 1.3866009972434372E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGCG 90 2.4109771E-5 31.6681 5 GTGTACG 65 0.005845314 29.228039 1 GCACCGT 210 4.3655746E-11 27.144087 6 TACCTGG 540 0.0 26.388254 2 GTACCTG 630 0.0 24.878628 1 GTACATG 3885 0.0 24.328487 1 TACATGG 3920 0.0 23.264746 2 ACATGGG 4000 0.0 22.32601 3 GTACACG 110 0.0028778967 21.588892 1 GTAAGCG 80 4.9647322E-5 20.795176 94-95 AGTACTT 2920 0.0 20.739363 12-13 AAGGTGT 490 0.0 20.358063 6 GAGTACT 2785 0.0 20.039217 12-13 AGGTAAG 310 2.582965E-10 19.918875 2 ACTAGGT 145 6.141117E-4 19.656063 3 ATAGCCC 195 1.0313683E-5 19.488062 3 GGTAAGG 320 3.947207E-10 19.297749 3 GTACTTT 2935 0.0 19.019024 14-15 ACAGCGC 125 0.00602985 18.99954 9 CATGGGC 705 0.0 18.866104 4 >>END_MODULE