FastQCFastQC Report
Thu 26 May 2016
SRR936451_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936451_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721188
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT55550.7702568539687293No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42570.5902760445265313No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT39580.5488166747089525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38600.5352279849359667No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT27610.382840535338913No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC23500.32585123435220775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22060.30588417999190226No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT20160.27953876104427694No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT18810.26081964758149057No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA14080.19523342041187597No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11010.15266476979650245No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC10710.14850496680477213No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC10670.14795032640587477No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA10080.13976938052213847No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG9880.13699617852765159No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC8230.11411726207313488No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA8190.11356262167423752No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG8080.11203736057726972No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA7510.10413373489298212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG3150.028.6508355
GTAAGGT2550.027.9412174
GTGTACG908.936984E-426.3925881
GCTTATG1701.1200973E-725.1505831
GTACATG36850.023.72111
GGTAAGG2853.6379788E-1223.3366033
GAGTACT43250.023.28085112-13
GTATGGT1652.4248402E-623.0335331
TACATGG37250.022.57522
ACATGGG36950.022.499773
GTACTTT45050.022.1932814-15
AGTACTT43500.022.00061612-13
GGGTACC2205.6359568E-821.5894437
TATAAGG2901.05501385E-1021.2975652
GGCTAGT1150.003718929820.655071
ATGGGAG8950.019.63695
CATGGGG14650.019.4539554
TACTTTT50450.019.11168514-15
ACTTTTT53050.018.98080316-17
CATGGGA22200.018.6148914
Produced by FastQC (version 0.11.5)