FastQCFastQC Report
Thu 26 May 2016
SRR936450_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936450_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819237
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT61430.7498440622188695No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT35920.43845675915516513No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32390.3953678849954287No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27300.33323690214187107No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23620.2883170559923441No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC23020.28099316803318214No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT18960.23143485950951923No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15200.18553849496543734No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC14970.18273100458109193No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT14640.1787028662035528No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC13610.16613019187365805No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG12270.1497735087648629No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11710.14293788000297838No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC10820.13207411286355475No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA10820.13207411286355475No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA10300.12572674329894767No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA9750.1190131793363825No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8280.10106965383643561No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT1858.933057E-925.6827226
GTAAGGT3400.023.756524
GAGTACT32050.023.34741612-13
TGCACCG1852.53307E-723.1144495
GATCGTG1252.2509474E-422.8062577
GTACATG48250.021.4696521
TACATGG48650.021.2905272
CGGTTAC905.1750812E-621.1091728-29
GTACTTT35450.021.0411614-15
ACATGGG47350.020.7712753
AGTACTT33350.020.7278112-13
GGTAAGG3451.8189894E-1220.6578433
GGGACCG1150.00371646420.6578435
GTAGTAC1856.5327877E-620.5461773
TAAGGTG4400.020.5169935
CACCGTC2551.1868906E-820.4958217
CATGGGG18500.019.7756964
GTACCTG6650.019.2933641
GATATAG3254.838512E-1019.0075381
AACGGTC757.7277474E-418.99825550-51
Produced by FastQC (version 0.11.5)