##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936450_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 819237 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.269776633623724 31.0 31.0 34.0 28.0 34.0 2 31.30454801235784 31.0 31.0 34.0 28.0 34.0 3 31.34254800503395 31.0 31.0 34.0 28.0 34.0 4 34.97480704606848 37.0 35.0 37.0 32.0 37.0 5 34.88670555651173 37.0 35.0 37.0 32.0 37.0 6 35.02724608385608 37.0 35.0 37.0 32.0 37.0 7 34.82349552083219 37.0 35.0 37.0 32.0 37.0 8 34.827839807039965 37.0 35.0 37.0 32.0 37.0 9 36.30412810944818 39.0 37.0 39.0 32.0 39.0 10-11 36.18557267310925 39.0 35.0 39.0 32.0 39.0 12-13 36.08213984475799 39.0 35.0 39.0 30.5 39.0 14-15 37.118734261269935 39.0 36.0 41.0 31.0 41.0 16-17 37.08554667330699 39.0 36.0 41.0 31.0 41.0 18-19 37.078938695395834 39.0 36.0 41.0 31.0 41.0 20-21 36.9902439709144 39.0 36.0 41.0 31.0 41.0 22-23 36.91330286595942 39.0 36.0 41.0 31.0 41.0 24-25 36.73993972440209 39.0 36.0 41.0 30.0 41.0 26-27 36.52094998150719 39.0 36.0 40.5 30.0 41.0 28-29 36.618723885761995 39.0 36.0 41.0 30.0 41.0 30-31 36.577134577661894 39.0 36.0 41.0 30.0 41.0 32-33 36.42213974710615 39.0 35.0 40.5 30.0 41.0 34-35 36.2306853816417 39.0 35.0 40.0 30.0 41.0 36-37 35.95762276362029 38.0 35.0 40.0 28.5 41.0 38-39 35.77085263482972 38.0 35.0 40.0 28.0 41.0 40-41 35.648259294929304 38.0 35.0 40.0 27.5 41.0 42-43 35.356087432574455 38.0 34.5 40.0 27.0 41.0 44-45 35.0595053690202 38.0 34.0 40.0 25.5 41.0 46-47 34.66877472575091 38.0 33.5 40.0 24.0 41.0 48-49 34.37350730008532 38.0 33.0 40.0 23.0 41.0 50-51 33.63789294184711 37.5 32.5 39.5 22.5 40.5 52-53 33.81936545834722 37.5 33.0 39.5 23.0 40.5 54-55 34.16392814289394 38.0 33.0 40.0 23.0 41.0 56-57 34.06850947894199 38.0 33.0 40.0 22.5 41.0 58-59 33.82764633433304 38.0 33.0 40.0 22.0 41.0 60-61 33.46536167189714 37.0 33.0 40.0 20.5 41.0 62-63 33.158244805837626 37.0 32.0 40.0 20.0 41.0 64-65 32.85159910990349 36.0 32.0 39.0 19.0 41.0 66-67 32.454035889492296 36.0 31.5 39.0 18.0 41.0 68-69 32.07497097909396 35.0 31.0 39.0 15.5 40.0 70-71 31.64015114063452 35.0 31.0 38.0 13.0 40.0 72-73 31.219233872493554 35.0 31.0 37.0 9.5 39.5 74-75 30.8246227892539 35.0 30.0 37.0 7.5 39.0 76-77 30.404312793489552 34.5 30.0 36.0 7.0 39.0 78-79 29.936424990570494 34.0 29.5 36.0 2.0 38.5 80-81 29.510799072795784 34.0 29.0 35.0 2.0 37.0 82-83 29.098368970151498 34.0 29.0 35.0 2.0 37.0 84-85 28.724921481817837 34.0 29.0 35.0 2.0 36.0 86-87 28.412192076285617 34.0 28.5 35.0 2.0 36.0 88-89 28.009315985484054 34.0 27.0 35.0 2.0 35.5 90-91 27.636815719993116 34.0 26.5 35.0 2.0 35.0 92-93 27.266099431544227 33.0 26.0 35.0 2.0 35.0 94-95 26.82206125944019 33.0 25.0 35.0 2.0 35.0 96-97 26.212848662841154 33.0 24.5 35.0 2.0 35.0 98-99 25.54632176036971 33.0 21.5 35.0 2.0 35.0 100-101 23.53363373480446 30.5 9.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30720564348733603 1101 2 0.42148779393645697 1101 3 0.3845360052048292 1101 4 0.4133195258859246 1101 5 0.4281230185467564 1101 6 0.4080057564826447 1101 7 0.37817130113052144 1101 8 0.17203875545902036 1101 9 0.6148224828117677 1101 10-11 0.3579461830609887 1101 12-13 0.4029170057418625 1101 14-15 0.4848076505442904 1101 16-17 0.6644304970667818 1101 18-19 0.7236942765445136 1101 20-21 0.3740577420077784 1101 22-23 0.565053828930381 1101 24-25 0.44687584048219975 1101 26-27 0.3720558310169366 1101 28-29 0.38079968737586967 1101 30-31 0.22916006782160991 1101 32-33 0.2659638353247047 1101 34-35 0.3255456729457009 1101 36-37 0.4304446137510425 1101 38-39 0.42212750048105363 1101 40-41 0.2743395291067614 1101 42-43 0.11573436974665441 1101 44-45 0.03644771429899407 1101 46-47 0.44384712714371943 1101 48-49 0.25209134176224524 1101 50-51 0.27748320604430887 1101 52-53 0.2818816769169459 1101 54-55 0.20370959909688224 1101 56-57 0.008683679570872016 1101 58-59 0.16536205030767093 1101 60-61 0.1197415676400837 1101 62-63 0.3185934995214481 1101 64-65 0.46161719882562835 1101 66-67 0.27364561081535754 1101 68-69 0.20011044582620485 1101 70-71 0.24681535803429355 1101 72-73 0.022865284188046786 1101 74-75 0.09986333028081518 1101 76-77 -0.2397428171435294 1101 78-79 -0.07143182795757852 1101 80-81 -0.03714814301095615 1101 82-83 0.10738987568630165 1101 84-85 0.10426096047580913 1101 86-87 -0.020402132703679854 1101 88-89 -0.016062732887043296 1101 90-91 0.03663181185130071 1101 92-93 0.011488371550594678 1101 94-95 -0.1087307377019684 1101 96-97 0.003973518679860177 1101 98-99 -0.13071940635822799 1101 100-101 0.17366753752537178 1102 1 0.13581651242078863 1102 2 0.1789953014439618 1102 3 0.2633018678416583 1102 4 0.2170103134476804 1102 5 0.42889798706365667 1102 6 0.32002261204788596 1102 7 0.1025246867742311 1102 8 0.10708670663600373 1102 9 0.4265293509702488 1102 10-11 0.33076174055396024 1102 12-13 0.49607064325033434 1102 14-15 0.29651451870277157 1102 16-17 0.31133546655239996 1102 18-19 0.2867815573737289 1102 20-21 -0.10970424578646742 1102 22-23 -0.25707878755707014 1102 24-25 0.10765758997363406 1102 26-27 -0.1693807668712779 1102 28-29 0.08294538145705133 1102 30-31 0.1824439969119851 1102 32-33 0.11723042449452237 1102 34-35 -0.046717719478984066 1102 36-37 0.03693013152687996 1102 38-39 -0.08540422479260457 1102 40-41 -0.17872644008824068 1102 42-43 0.04249984489923264 1102 44-45 -0.04672820355433771 1102 46-47 0.06697025026684145 1102 48-49 0.20605336830491439 1102 50-51 0.2923165878131755 1102 52-53 0.2607153492990051 1102 54-55 0.13580298280317038 1102 56-57 0.4222383632868443 1102 58-59 0.23418894171521032 1102 60-61 -0.24281050055714815 1102 62-63 0.01789360607961754 1102 64-65 0.3302842529765613 1102 66-67 0.0378493306642369 1102 68-69 0.20054636561696526 1102 70-71 0.3429719985457709 1102 72-73 -0.038841583970430804 1102 74-75 -0.11031055328016492 1102 76-77 -0.005871849401593465 1102 78-79 0.10768277051186104 1102 80-81 0.2163513241981896 1102 82-83 0.2182588091358788 1102 84-85 -0.12751517049386862 1102 86-87 -0.08727949393587764 1102 88-89 -0.5183392029054481 1102 90-91 -0.5226653885749322 1102 92-93 -0.4927739552923782 1102 94-95 -0.43516200768162605 1102 96-97 -0.644675129968789 1102 98-99 -0.7477033257615027 1102 100-101 -0.42751428946290915 1103 1 0.3836720727191789 1103 2 0.40376341061051235 1103 3 0.4877295090934979 1103 4 0.36910292072047923 1103 5 0.4983703803667723 1103 6 0.4124012123690406 1103 7 0.3377016284576655 1103 8 0.15452862382607435 1103 9 0.5134435259538179 1103 10-11 0.6232651791105184 1103 12-13 0.5710489617114902 1103 14-15 0.5160892217673165 1103 16-17 0.8079206241839572 1103 18-19 0.5975152741992815 1103 20-21 0.4773666121926752 1103 22-23 0.28061575343797784 1103 24-25 0.4170717818958991 1103 26-27 0.4018854349974035 1103 28-29 0.33414802931498144 1103 30-31 0.13258323304427222 1103 32-33 0.2700556306385309 1103 34-35 0.45399285907932807 1103 36-37 0.5621010430323281 1103 38-39 0.424838608442478 1103 40-41 0.26763715931823384 1103 42-43 0.3212820877189131 1103 44-45 0.2647584385850834 1103 46-47 0.6028379522269915 1103 48-49 0.38715519700721046 1103 50-51 0.00855860121508556 1103 52-53 0.25566592216591744 1103 54-55 0.54646657340151 1103 56-57 0.4728859298728807 1103 58-59 0.3477409198168502 1103 60-61 0.6057888381559238 1103 62-63 0.38122792185767906 1103 64-65 0.5003234152560978 1103 66-67 0.35538139562755333 1103 68-69 0.32172753520047337 1103 70-71 0.27820941139614774 1103 72-73 0.3037368930369695 1103 74-75 0.1668853800762058 1103 76-77 -0.04742926143891424 1103 78-79 0.3660149181360346 1103 80-81 0.5743550303862328 1103 82-83 0.4713433468109436 1103 84-85 0.29268914413236047 1103 86-87 0.3125899086699704 1103 88-89 0.11827143053425715 1103 90-91 0.23461232483577632 1103 92-93 0.011630107285963476 1103 94-95 0.0073903837243562975 1103 96-97 0.20786161007633908 1103 98-99 0.09833996278590362 1103 100-101 0.29081541898814933 1104 1 0.19616682727222567 1104 2 0.11186677688858282 1104 3 0.2261865284007385 1104 4 0.2973372133544743 1104 5 0.2082102393691585 1104 6 0.20562469438664976 1104 7 0.3177489473848425 1104 8 0.38697486049264995 1104 9 0.44213741965408104 1104 10-11 0.2924273463493563 1104 12-13 0.3228294162769316 1104 14-15 0.3559062605776333 1104 16-17 0.03125976580903256 1104 18-19 0.3933384996395972 1104 20-21 0.294877013164637 1104 22-23 0.22494607471910655 1104 24-25 0.4238389080304117 1104 26-27 0.2668286642743922 1104 28-29 0.31694895810878876 1104 30-31 0.20586462479623435 1104 32-33 -0.009143024928441434 1104 34-35 0.11287994508867172 1104 36-37 0.2538915688559058 1104 38-39 0.30743451519541765 1104 40-41 0.11392058530447713 1104 42-43 0.12157643222787584 1104 44-45 -0.1925875850663914 1104 46-47 -8.440517935142111E-4 1104 48-49 -0.33407977104052833 1104 50-51 -0.37353074523220187 1104 52-53 -0.19046183893745194 1104 54-55 0.13760646397648912 1104 56-57 -0.055937051779046953 1104 58-59 -0.1636302100424345 1104 60-61 -0.3020421645337379 1104 62-63 -0.4906303052012291 1104 64-65 -0.3363293701261867 1104 66-67 -0.44395577970426103 1104 68-69 -0.3165374507916887 1104 70-71 -0.1682950227550073 1104 72-73 -0.40633876129506774 1104 74-75 -0.5476973621035803 1104 76-77 -0.5020356630822889 1104 78-79 -0.3987942275255314 1104 80-81 -0.2529468715628731 1104 82-83 -0.46015511252635477 1104 84-85 -0.3225478301739422 1104 86-87 -0.7498968528210561 1104 88-89 -0.5047467896506177 1104 90-91 -0.523376740655376 1104 92-93 -0.9267953394262918 1104 94-95 -0.6532222045490244 1104 96-97 -0.4845953372933458 1104 98-99 -0.5259101880831416 1104 100-101 -0.3518457511162012 1105 1 -0.16495586377185134 1105 2 0.08223372842159904 1105 3 -0.029734125898560393 1105 4 0.006537800953360318 1105 5 0.2460518060348349 1105 6 0.1444206129565586 1105 7 0.19649712354539872 1105 8 0.011107606227099609 1105 9 0.2371204964091902 1105 10-11 0.33690793672742103 1105 12-13 0.16361443647564045 1105 14-15 0.05731768897890532 1105 16-17 0.16339135024163198 1105 18-19 0.1016321303456067 1105 20-21 0.03878886340632448 1105 22-23 -0.2316105660416028 1105 24-25 -0.1943074259282369 1105 26-27 0.020709596095052518 1105 28-29 0.021546522352601016 1105 30-31 -0.07145574562927948 1105 32-33 -0.062126181088792976 1105 34-35 -0.17146530895851697 1105 36-37 -0.3302072807604617 1105 38-39 -0.1611904835638711 1105 40-41 -0.29747927970660015 1105 42-43 -0.286498337745023 1105 44-45 0.05989631055540201 1105 46-47 -0.39281444355919604 1105 48-49 -0.4680215471375604 1105 50-51 -0.29443690807252665 1105 52-53 -0.34089881597940064 1105 54-55 -0.4277539862512967 1105 56-57 -0.35626509473728873 1105 58-59 -0.2557651195700643 1105 60-61 -0.22558979279803992 1105 62-63 -0.2809036577903399 1105 64-65 -0.35848095475730446 1105 66-67 -0.48988427527245904 1105 68-69 -0.423677472032594 1105 70-71 -0.4190679832806694 1105 72-73 -0.14064505713919573 1105 74-75 -0.26724048411968226 1105 76-77 -0.4244505257608253 1105 78-79 -0.4752403957257094 1105 80-81 -0.17666447670039176 1105 82-83 -0.11728942651458141 1105 84-85 -0.5158487562377267 1105 86-87 -0.3021628802754215 1105 88-89 -0.1538016189789495 1105 90-91 0.06768495722748114 1105 92-93 -0.3629150527733209 1105 94-95 -0.3595145746966182 1105 96-97 -0.1680735852164048 1105 98-99 -0.3097269009041135 1105 100-101 -0.26420468271849984 1106 1 0.18509300440623733 1106 2 -0.04636811809741559 1106 3 -0.06652051960154637 1106 4 0.12053591603792313 1106 5 0.28957939037607616 1106 6 0.10128135610192857 1106 7 0.3917080420926169 1106 8 0.24221481951924062 1106 9 0.26606180223672027 1106 10-11 0.21199083810096653 1106 12-13 0.2790698704425125 1106 14-15 0.4171358295447263 1106 16-17 0.3164120144997966 1106 18-19 0.09377613439468746 1106 20-21 0.1955531791645697 1106 22-23 0.09047654148584172 1106 24-25 0.2492912790650479 1106 26-27 0.11975928456041629 1106 28-29 -0.12600919609688077 1106 30-31 0.07140950036964 1106 32-33 -0.06988400436052444 1106 34-35 -0.3441837758939599 1106 36-37 0.08755237506794344 1106 38-39 0.008015583071376398 1106 40-41 -0.2123635668420505 1106 42-43 -0.11163534317288537 1106 44-45 -0.15523275379351276 1106 46-47 -0.09725141233591472 1106 48-49 -0.07291360082273712 1106 50-51 0.0013661008110048556 1106 52-53 -0.23476106007595376 1106 54-55 0.100860077312241 1106 56-57 0.11609820526097536 1106 58-59 0.13862725699414824 1106 60-61 -0.07703489463583679 1106 62-63 -0.006548106888899952 1106 64-65 -0.03538375944166461 1106 66-67 -0.07629804911750426 1106 68-69 0.09110078860933157 1106 70-71 0.057830894496525787 1106 72-73 0.2523270879992907 1106 74-75 -0.04459357415930043 1106 76-77 -0.13721253773352515 1106 78-79 0.0795552305723497 1106 80-81 0.02592248247722395 1106 82-83 0.10025904897279148 1106 84-85 -0.08123830542877997 1106 86-87 0.2436204940722746 1106 88-89 0.40833732372311005 1106 90-91 0.33937675610002316 1106 92-93 0.12955619353974512 1106 94-95 0.14829124410715977 1106 96-97 0.09803982386260657 1106 98-99 0.34438238928559883 1106 100-101 0.5547910762217576 1107 1 0.45592665129153787 1107 2 0.5133698026266131 1107 3 0.2554296231273092 1107 4 0.4296479870361196 1107 5 0.3710351745319187 1107 6 0.5050058517849436 1107 7 0.5146530225018893 1107 8 0.5077005240579666 1107 9 0.6757312432111888 1107 10-11 0.6647172351631099 1107 12-13 0.8131316477052337 1107 14-15 0.6020374243954549 1107 16-17 0.7745064212343848 1107 18-19 0.8585532114883421 1107 20-21 1.099434450831005 1107 22-23 1.1469723348726575 1107 24-25 1.10779907271273 1107 26-27 0.8340946296431682 1107 28-29 0.9507533422191727 1107 30-31 0.939278701539564 1107 32-33 1.056732042476618 1107 34-35 0.850378982159306 1107 36-37 1.0123375292602503 1107 38-39 1.0819335779441772 1107 40-41 0.8761797459724434 1107 42-43 1.340625742770456 1107 44-45 0.9786062761519503 1107 46-47 1.074951327857427 1107 48-49 0.5097587649644737 1107 50-51 0.4495020970784793 1107 52-53 0.7467230691776905 1107 54-55 1.0403420392946927 1107 56-57 0.9677831081100265 1107 58-59 0.8961612836535906 1107 60-61 0.6236860240463429 1107 62-63 1.0276867510595196 1107 64-65 0.9499859057851765 1107 66-67 1.0720609453087917 1107 68-69 0.9852531698483844 1107 70-71 1.0370699916675363 1107 72-73 1.0663054855229888 1107 74-75 0.9487077016209433 1107 76-77 0.944500359035132 1107 78-79 0.9693743236295553 1107 80-81 1.0926827130508023 1107 82-83 1.206352492475741 1107 84-85 1.2974246086479475 1107 86-87 0.7793077247311366 1107 88-89 1.031052784092651 1107 90-91 1.298404293487959 1107 92-93 1.3786907901112464 1107 94-95 1.4932586096184508 1107 96-97 1.2121225714808652 1107 98-99 1.391267187784564 1107 100-101 1.959807406008192 1108 1 0.07269146542477145 1108 2 -0.0993464285106711 1108 3 -0.08032672578693578 1108 4 0.028838270436928326 1108 5 0.04521625660773765 1108 6 -0.10393783610611251 1108 7 -0.12033041511313058 1108 8 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-2.449167844135406 2316 64-65 -2.3192599436188956 2316 66-67 -3.1329132055000386 2316 68-69 -2.593112281297177 2316 70-71 -2.3429512625061264 2316 72-73 -2.2642820735114597 2316 74-75 -2.425646955668814 2316 76-77 -3.1616986566730887 2316 78-79 -3.173723214931332 2316 80-81 -3.0876575311415593 2316 82-83 -2.989288805199781 2316 84-85 -3.02853067597702 2316 86-87 -3.157401675007005 2316 88-89 -3.2414095775689376 2316 90-91 -3.2828121974799025 2316 92-93 -3.537705616194753 2316 94-95 -3.771852611094964 2316 96-97 -3.72638048181069 2316 98-99 -3.982174675486167 2316 100-101 -5.0047275746714455 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2130.0 3 800.0 4 813.0 5 905.0 6 936.0 7 1130.0 8 1329.0 9 1771.0 10 2301.0 11 2959.0 12 3513.0 13 4318.0 14 4886.0 15 6012.0 16 7427.0 17 8869.0 18 7889.0 19 5685.0 20 5744.0 21 6332.0 22 6809.0 23 7671.0 24 8764.0 25 10265.0 26 11746.0 27 14032.0 28 16658.0 29 19811.0 30 24326.0 31 29355.0 32 36691.0 33 46216.0 34 59883.0 35 80938.0 36 109759.0 37 138371.0 38 105661.0 39 16521.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.6117389812754 18.41852413003322 14.067927181982778 21.9018097067086 2 15.582121546529685 20.829345586296473 39.28374366436517 24.304789202808678 3 20.860703617211144 24.732807840159577 29.464932059030698 24.941556483598582 4 12.739278924406358 16.369321990644355 37.53539502619751 33.35600405875178 5 13.572107200339978 38.09895979620896 33.52917040145174 14.799762601999323 6 31.19279726489546 36.10027739471276 17.297044421019457 15.409880919372313 7 28.45378951609949 33.38266358963088 20.431883951793107 17.731662942476525 8 26.791797621925756 32.79742411921141 19.59298250755206 20.817795751310776 9 26.47968003750476 17.680955590065146 20.80195727973278 35.037407092697315 10-11 25.421599859797478 26.417428590445592 26.42243582345473 21.738535726302203 12-13 27.34286460979327 24.035901656961975 26.84991597952245 21.771317753722304 14-15 23.46212992068061 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21.742068149732386 40-41 22.279825002563438 26.922640293355077 28.89526764744657 21.902267056634912 42-43 21.901135393353403 27.216543797449194 28.016012567876114 22.86630824132129 44-45 22.373897201309777 26.765622282102846 28.06563540430103 22.794845112286342 46-47 23.11758160803364 27.35687199365727 27.57250775293867 21.953038645370423 48-49 22.89494660236246 27.340823284275128 27.492001645445548 22.272228467916865 50-51 22.103267026034647 27.76825647936564 28.083252768500618 22.0452237260991 52-53 22.870969454798022 28.021608134970887 26.98239868363955 22.125023726591543 54-55 21.914771548198875 27.32382417206821 27.656705850758655 23.104698428974256 56-57 22.75696014794606 27.55342750856763 27.12240644273346 22.567205900752853 58-59 23.552884952835516 26.976071386065193 26.920713006167546 22.550330654931745 60-61 22.54047558159903 27.316371439714317 28.3231723505713 21.819980628115353 62-63 22.291874815755634 27.564971506589465 27.95949095760686 22.183662720048044 64-65 21.96809341751261 28.20762593932647 27.466235516428146 22.35804512673278 66-67 22.162313922474564 27.752482559523443 27.010735887404408 23.074467630597585 68-69 22.048777689071837 27.625044861606522 27.415690697839075 22.91048675148257 70-71 22.71465185166196 27.59178655943611 27.0370173694211 22.65654421948083 72-73 22.51603243902424 27.14268885990392 27.782582609709365 22.55869609136247 74-75 21.892669505180613 28.747399927735078 27.009868067694654 22.350062499389654 76-77 22.399125992566056 27.631085856582217 27.010430717210987 22.959357433640744 78-79 22.42211000666089 27.907362940414025 27.094074859623706 22.576452193301378 80-81 22.783631714179496 27.89180761593022 26.873261344261312 22.451299325628966 82-83 22.452589387566764 27.71943830532123 27.41792444941678 22.410047857695226 84-85 22.225423329479796 28.076715077988336 27.164806429993615 22.533055162538258 86-87 22.453264582158386 28.023245026160115 26.906831903865548 22.616658487815954 88-89 22.126145107706808 28.0880599957397 26.385893896604152 23.39990099994934 90-91 22.28678373196454 27.673630885998374 26.89089633879471 23.148689043242378 92-93 22.738052000769116 28.005762263886048 26.983692915284347 22.272492820060492 94-95 22.258410260041163 27.82777369509435 26.989921604339962 22.923894440524524 96-97 21.6895487188126 27.856289237928177 27.502713009383534 22.95144903387569 98-99 22.038525449823755 28.294668289699075 26.303747178109088 23.363059082368082 100-101 21.893096230732972 27.55609449911221 26.936594348908148 23.614214921246667 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 251.0 1 175.5 2 158.0 3 211.5 4 206.0 5 219.0 6 288.5 7 463.0 8 715.5 9 878.0 10 857.5 11 806.0 12 870.0 13 994.5 14 1086.0 15 1100.5 16 1160.0 17 1271.5 18 1335.5 19 1420.5 20 1603.0 21 1853.0 22 2164.5 23 2572.5 24 2963.5 25 3577.0 26 4539.5 27 5685.5 28 6785.5 29 8004.0 30 9482.5 31 10990.5 32 12963.5 33 14308.0 34 15337.5 35 16424.0 36 17970.0 37 19880.0 38 20673.0 39 21016.5 40 21436.0 41 22792.0 42 24139.5 43 25308.5 44 26351.0 45 27763.5 46 28997.5 47 33416.0 48 44834.0 49 44222.5 50 33513.0 51 29086.0 52 26838.5 53 26203.5 54 26118.0 55 24360.5 56 21958.0 57 19852.5 58 18130.5 59 16152.5 60 13679.0 61 11354.5 62 9403.0 63 7353.5 64 5429.0 65 4197.5 66 3141.0 67 2425.0 68 1809.5 69 1234.0 70 950.0 71 771.0 72 640.5 73 578.5 74 467.0 75 370.5 76 283.0 77 173.0 78 104.0 79 74.5 80 58.0 81 37.0 82 25.0 83 16.5 84 8.0 85 8.0 86 8.5 87 6.0 88 2.5 89 3.0 90 3.0 91 2.0 92 2.0 93 3.0 94 3.0 95 1.0 96 0.0 97 1.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01879797909518247 2 0.007445952758481368 3 0.008544535952355667 4 0.032469236618951534 5 0.04382126295565264 6 0.06689151002701293 7 0.03259130141827091 8 0.031126523826438504 9 0.017211136704030704 10-11 0.05145031291311305 12-13 0.04748320693523364 14-15 0.02520638105944922 16-17 0.028258001042433388 18-19 0.032530269018611224 20-21 0.024229862664894287 22-23 0.016295650709135454 24-25 0.0043943327754972 26-27 0.0039060735782197337 28-29 0.0021971663877486 30-31 0.0084224711530363 32-33 0.0010985831938743 34-35 0.002685425585026067 36-37 0.0062253047652877005 38-39 0.006774596362224851 40-41 0.003051619982984167 42-43 6.713563962565168E-4 44-45 0.0017699395901308167 46-47 0.0038450411785600504 48-49 7.323887959162001E-4 50-51 0.0025633607857067 52-53 0.0010375507942146167 54-55 0.0032957495816229006 56-57 0.0023802635867276505 58-59 0.0036009115799213167 60-61 0.0010375507942146167 62-63 0.0015258099914920835 64-65 0.0040281383775391 66-67 0.002685425585026067 68-69 0.0062253047652877005 70-71 0.008239373954057251 72-73 0.004699494773795617 74-75 0.0035398791802616334 76-77 0.002685425585026067 78-79 0.033872981811124254 80-81 0.021666501879187585 82-83 0.004577429974476251 84-85 0.009276924748271867 86-87 0.0062253047652877005 88-89 0.0055539483690311845 90-91 0.01483087311730305 92-93 0.013976419522067485 94-95 0.0072018231598426335 96-97 0.0043943327754972 98-99 0.023680571067957134 100-101 0.0428447445610977 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 819237.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.30537929795268 #Duplication Level Percentage of deduplicated Percentage of total 1 85.40693072278945 54.921250748029436 2 8.978542347742447 11.547371424286172 3 2.356325845805965 4.545732817923652 4 0.9888383021345756 2.543504883324297 5 0.5481001074162802 1.7622892650326256 6 0.32105586638100425 1.2387371558077978 7 0.21772951525239037 0.9800825343865035 8 0.16504775161633947 0.8490766615970393 9 0.1358944044100869 0.7864867098054013 >10 0.7861235728794282 9.487754539708506 >50 0.059680678021996586 2.6110203579839752 >100 0.030409264310021063 3.673367815003939 >500 0.002280694817169895 0.9348413087118782 >1k 0.002850868521462369 3.3677019560146166 >5k 1.9005790143082458E-4 0.7507818223842421 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6143 0.7498440622188695 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3592 0.43845675915516513 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3239 0.3953678849954287 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2730 0.33323690214187107 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2362 0.2883170559923441 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2302 0.28099316803318214 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1896 0.23143485950951923 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1520 0.18553849496543734 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1497 0.18273100458109193 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1464 0.1787028662035528 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1361 0.16613019187365805 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1227 0.1497735087648629 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1171 0.14293788000297838 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1082 0.13207411286355475 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1082 0.13207411286355475 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1030 0.12572674329894767 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 975 0.1190131793363825 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 828 0.10106965383643561 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 1.2206479931936668E-4 0.0 0.0 0.0 5 0.0 1.2206479931936668E-4 0.0 0.0 0.0 6 0.0 1.2206479931936668E-4 0.0 0.0 0.0 7 0.0 1.2206479931936668E-4 0.0 0.0 0.0 8 0.0 1.2206479931936668E-4 0.0 0.0 0.0 9 0.0 1.2206479931936668E-4 0.0 0.0 0.0 10-11 0.0 1.2206479931936668E-4 0.0 0.0 0.0 12-13 0.0 1.2206479931936668E-4 0.0 1.2206479931936668E-4 0.0 14-15 0.0 1.2206479931936668E-4 0.0 1.2206479931936668E-4 0.0 16-17 0.0 1.2206479931936668E-4 0.0 1.2206479931936668E-4 0.0 18-19 0.0 1.2206479931936668E-4 0.0 1.8309719897905002E-4 0.0 20-21 0.0 1.2206479931936668E-4 0.0 2.4412959863873336E-4 0.0 22-23 0.0 1.2206479931936668E-4 0.0 3.0516199829841667E-4 0.0 24-25 0.0 1.2206479931936668E-4 0.0 5.4929159693715E-4 2.4412959863873336E-4 26-27 0.0 1.2206479931936668E-4 0.0 9.154859948952501E-4 2.4412959863873336E-4 28-29 6.103239965968334E-5 1.2206479931936668E-4 0.0 0.001769939590130817 2.4412959863873336E-4 30-31 1.2206479931936668E-4 1.2206479931936668E-4 0.0 0.0029295551836648003 2.4412959863873336E-4 32-33 1.2206479931936668E-4 1.2206479931936668E-4 0.0 0.006591499163245801 3.6619439795810004E-4 34-35 1.2206479931936668E-4 1.2206479931936668E-4 0.0 0.009154859948952501 3.6619439795810004E-4 36-37 1.2206479931936668E-4 1.2206479931936668E-4 0.0 0.012206479931936668 3.6619439795810004E-4 38-39 1.2206479931936668E-4 1.2206479931936668E-4 0.0 0.020751015884292335 3.6619439795810004E-4 40-41 1.2206479931936668E-4 1.2206479931936668E-4 0.0 0.031370653425077236 3.6619439795810004E-4 42-43 1.2206479931936668E-4 1.2206479931936668E-4 0.0 0.052121669309369574 3.6619439795810004E-4 44-45 1.2206479931936668E-4 1.8309719897905002E-4 0.0 0.07372713878889747 3.6619439795810004E-4 46-47 1.2206479931936668E-4 2.4412959863873336E-4 0.0 0.11553433255578055 3.6619439795810004E-4 48-49 1.2206479931936668E-4 2.4412959863873336E-4 0.0 0.15575468393151187 3.6619439795810004E-4 50-51 1.2206479931936668E-4 2.4412959863873336E-4 0.0 0.18480610616952114 3.6619439795810004E-4 52-53 1.2206479931936668E-4 2.4412959863873336E-4 0.0 0.22118141636669242 4.272267976177834E-4 54-55 1.2206479931936668E-4 2.4412959863873336E-4 0.0 0.262317253737319 4.882591972774667E-4 56-57 1.2206479931936668E-4 2.4412959863873336E-4 0.0 0.3420866000925251 4.882591972774667E-4 58-59 1.2206479931936668E-4 2.4412959863873336E-4 0.0 0.4283864132113174 4.882591972774667E-4 60-61 1.2206479931936668E-4 2.4412959863873336E-4 0.0 0.5202401746991407 4.882591972774667E-4 62-63 1.2206479931936668E-4 2.4412959863873336E-4 0.0 0.6114836121903673 4.882591972774667E-4 64-65 1.2206479931936668E-4 2.4412959863873336E-4 0.0 0.7092575164451801 4.882591972774667E-4 66-67 1.2206479931936668E-4 2.4412959863873336E-4 0.0 0.8592751548086817 4.882591972774667E-4 68-69 1.2206479931936668E-4 2.4412959863873336E-4 0.0 1.0503065657434907 4.882591972774667E-4 70-71 1.2206479931936668E-4 2.4412959863873336E-4 0.0 1.1893994045679088 6.103239965968333E-4 72-73 1.2206479931936668E-4 2.4412959863873336E-4 0.0 1.3093280698991867 6.103239965968333E-4 74-75 1.2206479931936668E-4 2.4412959863873336E-4 0.0 1.4488481355212228 6.103239965968333E-4 76-77 1.2206479931936668E-4 2.4412959863873336E-4 0.0 1.6142459385989647 6.103239965968333E-4 78-79 1.2206479931936668E-4 2.4412959863873336E-4 0.0 1.791300930011706 6.103239965968333E-4 80-81 1.2206479931936668E-4 2.4412959863873336E-4 0.0 2.0650922748850453 6.713563962565167E-4 82-83 1.2206479931936668E-4 2.4412959863873336E-4 0.0 2.3701932407838022 7.323887959162001E-4 84-85 1.2206479931936668E-4 2.4412959863873336E-4 0.0 2.764035315787739 7.323887959162001E-4 86-87 1.2206479931936668E-4 2.4412959863873336E-4 0.0 3.165750570347775 7.323887959162001E-4 88-89 1.2206479931936668E-4 2.4412959863873336E-4 0.0 3.633747010938227 7.323887959162001E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCACCGT 185 8.933057E-9 25.682722 6 GTAAGGT 340 0.0 23.75652 4 GAGTACT 3205 0.0 23.347416 12-13 TGCACCG 185 2.53307E-7 23.114449 5 GATCGTG 125 2.2509474E-4 22.806257 7 GTACATG 4825 0.0 21.469652 1 TACATGG 4865 0.0 21.290527 2 CGGTTAC 90 5.1750812E-6 21.10917 28-29 GTACTTT 3545 0.0 21.04116 14-15 ACATGGG 4735 0.0 20.771275 3 AGTACTT 3335 0.0 20.72781 12-13 GGTAAGG 345 1.8189894E-12 20.657843 3 GGGACCG 115 0.003716464 20.657843 5 GTAGTAC 185 6.5327877E-6 20.546177 3 TAAGGTG 440 0.0 20.516993 5 CACCGTC 255 1.1868906E-8 20.495821 7 CATGGGG 1850 0.0 19.775696 4 GTACCTG 665 0.0 19.293364 1 GATATAG 325 4.838512E-10 19.007538 1 AACGGTC 75 7.7277474E-4 18.998255 50-51 >>END_MODULE