FastQCFastQC Report
Thu 26 May 2016
SRR936450_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936450_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819237
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT62040.7572900149773509No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46990.5735824920017041No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41710.5091322779610784No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT38590.47104806057343596No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT28720.3505701036452211No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC25290.30870187747867833No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23260.2839227232168469No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT22060.2692749472985229No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT19300.23558506268637772No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA14330.17491885742465246No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC12540.15306925834648583No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12140.14818666637371117No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC11990.14635569438392065No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG11260.13744496403360687No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA11260.13744496403360687No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC9010.10998038418674938No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA8760.10692876420376521No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG8200.10009313544188068No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTACG950.00115321925.2646681
GTACATG41600.023.7706531
TACATGG41700.023.3550262
ACATGGG41250.023.2586173
GTACACG1252.0998825E-423.0413781
GAGTACT48350.022.54185712-13
GTACTTT51200.021.1943314-15
AGTACTT48900.021.02579912-13
CATGGGA25150.020.9611434
CCTATAA4250.020.3306271
GTGTAGG2902.1354936E-919.8632561
CATGGGG16500.019.2850514
TACCTGG6000.019.0030162
ATGGGAT11000.018.9960585
GGGCGTA1250.00603692718.9960589
GAGTACG1300.00711563618.4626431
ACTTTTT59950.018.1801316-17
AGAGTAC80850.018.03274310-11
ATGGGTA3208.281859E-917.8088045
GTAAGGT2956.34227E-817.7092694
Produced by FastQC (version 0.11.5)