##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936450_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 819237 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.45010540295421 31.0 31.0 34.0 30.0 34.0 2 31.641041603345553 31.0 31.0 34.0 30.0 34.0 3 31.91070837864989 31.0 31.0 34.0 30.0 34.0 4 35.73110467422736 37.0 35.0 37.0 35.0 37.0 5 35.54806484570399 37.0 35.0 37.0 33.0 37.0 6 35.654157466032416 37.0 35.0 37.0 33.0 37.0 7 35.586044087364215 37.0 35.0 37.0 33.0 37.0 8 35.57093734780045 37.0 35.0 37.0 33.0 37.0 9 37.18113195571001 39.0 37.0 39.0 33.0 39.0 10-11 37.07137861204999 39.0 37.0 39.0 33.0 39.0 12-13 36.934181439559005 39.0 37.0 39.0 33.0 39.0 14-15 38.00884969795065 40.0 37.0 41.0 33.0 41.0 16-17 37.93346980177897 40.0 37.0 41.0 33.0 41.0 18-19 38.02763852218588 40.0 37.0 41.0 33.0 41.0 20-21 38.10956719484105 40.0 37.0 41.0 33.0 41.0 22-23 38.034424714704045 40.0 37.0 41.0 33.0 41.0 24-25 37.90760671209918 40.0 37.0 41.0 33.0 41.0 26-27 37.67471012661781 39.5 37.0 41.0 32.0 41.0 28-29 37.58609108231195 39.0 36.5 41.0 32.0 41.0 30-31 37.551917821094506 39.0 36.5 41.0 32.0 41.0 32-33 37.33364032630362 39.0 36.0 41.0 32.0 41.0 34-35 37.12665870804175 39.0 36.0 41.0 31.0 41.0 36-37 36.852153284092395 39.0 35.0 41.0 31.0 41.0 38-39 36.67261122239352 39.0 35.0 41.0 30.0 41.0 40-41 36.445345486104756 39.0 35.0 40.5 30.0 41.0 42-43 36.179416334955576 39.0 35.0 40.0 30.0 41.0 44-45 35.83512768588332 38.5 35.0 40.0 29.0 41.0 46-47 35.36170363399114 38.0 35.0 40.0 27.5 41.0 48-49 34.798203084089224 38.0 34.0 40.0 24.5 41.0 50-51 34.566394095969784 38.0 34.0 40.0 24.0 41.0 52-53 34.293120916169556 38.0 33.5 40.0 23.5 41.0 54-55 34.032540644526556 38.0 33.0 40.0 23.0 41.0 56-57 33.730220314756295 37.5 33.0 40.0 22.5 41.0 58-59 33.3489417592223 37.0 32.5 40.0 21.0 41.0 60-61 32.94166523240527 36.5 32.0 39.0 20.0 41.0 62-63 32.66886200208243 36.0 32.0 39.0 19.5 40.0 64-65 32.23166190003625 35.5 31.0 39.0 18.0 40.0 66-67 31.81419845539203 35.0 31.0 38.5 16.0 40.0 68-69 31.344432685535445 35.0 30.5 38.0 12.5 40.0 70-71 30.83357746293197 34.5 30.0 37.0 8.0 39.0 72-73 30.407479154383896 34.0 29.0 36.5 7.5 39.0 74-75 30.048479866021676 34.0 29.0 36.0 7.0 39.0 76-77 28.634451324830298 32.5 27.0 35.0 3.5 37.0 78-79 29.428654955769822 34.0 29.0 35.0 2.0 37.0 80-81 29.25170921235247 34.0 29.0 35.0 2.0 37.0 82-83 28.900289537703983 34.0 29.0 35.0 2.0 36.5 84-85 28.457956000522437 34.0 28.5 35.0 2.0 36.0 86-87 28.10523084284523 33.0 27.0 35.0 2.0 36.0 88-89 27.690036582820355 33.0 27.0 35.0 2.0 35.0 90-91 27.216717506655584 33.0 26.0 35.0 2.0 35.0 92-93 26.753383331075135 32.5 25.0 34.5 2.0 35.0 94-95 26.191331080017136 32.0 24.5 34.0 2.0 35.0 96-97 25.47824988373328 32.0 21.5 34.0 2.0 35.0 98-99 24.64934017872728 32.0 13.0 34.0 2.0 35.0 100-101 21.921354260122527 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4372379082041675 1101 2 0.3399331034773816 1101 3 0.10077839331275129 1101 4 0.06361461854292827 1101 5 0.19894884351694486 1101 6 0.14707052141302768 1101 7 0.1691352092515217 1101 8 0.19434948308940392 1101 9 0.23393659588853666 1101 10-11 0.2838288296915863 1101 12-13 0.20843328622400747 1101 14-15 0.35159344545709104 1101 16-17 0.2608302140013592 1101 18-19 0.34268161563529986 1101 20-21 0.03944827946997265 1101 22-23 0.10477431139012339 1101 24-25 0.11527659535663304 1101 26-27 0.0668344478809928 1101 28-29 0.18577111323671858 1101 30-31 0.110498534310004 1101 32-33 0.20001867818687202 1101 34-35 0.2310882612707914 1101 36-37 0.128777588124521 1101 38-39 0.3096488430038562 1101 40-41 0.34500031583905866 1101 42-43 0.18885002356810077 1101 44-45 0.18997677406868974 1101 46-47 0.36753359785397777 1101 48-49 0.3310630593392361 1101 50-51 0.029432210010973847 1101 52-53 0.05985720421477936 1101 54-55 0.207758799551776 1101 56-57 0.13660313258800016 1101 58-59 0.23165967128108633 1101 60-61 0.20526221795383748 1101 62-63 0.005919445778246768 1101 64-65 -0.017683489595732738 1101 66-67 -0.46004879228549456 1101 68-69 -0.21825551484008798 1101 70-71 -0.1300332414973262 1101 72-73 -0.3524502707778261 1101 74-75 -0.09798238994324038 1101 76-77 0.2639078665478536 1101 78-79 -0.008976878668935484 1101 80-81 -0.09834319936995328 1101 82-83 0.005509684011759219 1101 84-85 0.2673927029714882 1101 86-87 -0.006306915637786403 1101 88-89 -0.09325868225921852 1101 90-91 -0.13379735076245325 1101 92-93 0.05592255455374229 1101 94-95 -0.13673000280638092 1101 96-97 -0.1343027630622622 1101 98-99 -0.19082547043562315 1101 100-101 -0.22173322406606033 1102 1 0.4238697012875754 1102 2 0.4592540373144445 1102 3 0.24216171211542203 1102 4 0.1303829998274395 1102 5 0.11890912638045847 1102 6 0.15021883101294975 1102 7 0.19136711858020305 1102 8 0.11016852794070786 1102 9 0.14762447731835238 1102 10-11 0.12169088529557825 1102 12-13 0.11782251416162381 1102 14-15 0.31392271020570917 1102 16-17 0.2706505181764953 1102 18-19 0.14327252912944033 1102 20-21 0.14141718385823765 1102 22-23 -0.0014690287241805322 1102 24-25 0.05039719983207647 1102 26-27 -0.1721222507531195 1102 28-29 -0.04939550499441481 1102 30-31 -0.10158855277708057 1102 32-33 0.16748210935998742 1102 34-35 0.22266047864945193 1102 36-37 0.3211756313290124 1102 38-39 0.15503051499843679 1102 40-41 0.2626478020349339 1102 42-43 0.04993691691306168 1102 44-45 0.046886102752210945 1102 46-47 0.03013167980689957 1102 48-49 0.07734563223470303 1102 50-51 0.15999018734314774 1102 52-53 0.061491903430123784 1102 54-55 0.24534477262162113 1102 56-57 0.2787977465568048 1102 58-59 0.1479630714554574 1102 60-61 0.20510480247384066 1102 62-63 0.3581547455941916 1102 64-65 0.2788509480677348 1102 66-67 0.34832813302691434 1102 68-69 0.32054134653741784 1102 70-71 -0.04479881352095916 1102 72-73 0.04675538649234667 1102 74-75 -0.3862706782315257 1102 76-77 -0.1983366360192278 1102 78-79 -0.054821109997039486 1102 80-81 -0.07506992609667762 1102 82-83 0.10132727579709311 1102 84-85 -0.02257872006445183 1102 86-87 -0.038940355529287984 1102 88-89 -0.059002652035445635 1102 90-91 0.16236171152564083 1102 92-93 0.3977684326899471 1102 94-95 0.012403001165335326 1102 96-97 -0.05594618067341983 1102 98-99 -0.26665129626145045 1102 100-101 -0.48964226213638895 1103 1 0.3586726364258972 1103 2 0.5428854918069277 1103 3 0.31002154096677614 1103 4 0.1736122617743021 1103 5 0.06104821345216038 1103 6 0.16633092456878984 1103 7 0.23103417144928073 1103 8 0.15104839188904862 1103 9 0.0652414051798118 1103 10-11 0.20519021791071168 1103 12-13 0.20761542158822266 1103 14-15 0.2552733006888701 1103 16-17 0.11496436522678977 1103 18-19 0.27175936098232967 1103 20-21 0.29071236445867754 1103 22-23 0.28510501711871683 1103 24-25 0.18925729292718074 1103 26-27 0.18973416325586356 1103 28-29 0.34042416558170174 1103 30-31 0.32115656718280405 1103 32-33 0.08900086727881984 1103 34-35 0.10961496774432078 1103 36-37 0.12334259950458204 1103 38-39 0.12673187212725878 1103 40-41 0.021441529294726536 1103 42-43 0.1941123747661777 1103 44-45 0.32988354162043265 1103 46-47 0.029323703710787186 1103 48-49 0.02535143882182922 1103 50-51 -0.28730842842610826 1103 52-53 -0.21735894113356125 1103 54-55 -0.11034934151813758 1103 56-57 -0.10843199644920531 1103 58-59 0.03844419835343871 1103 60-61 -0.09437907526938005 1103 62-63 -0.32134383494255303 1103 64-65 -0.14471908669047906 1103 66-67 -0.03128088784833949 1103 68-69 -0.10911899860557739 1103 70-71 -0.021144762799711003 1103 72-73 -0.09768770285591799 1103 74-75 4.7038321530834537E-4 1103 76-77 0.005905153791804452 1103 78-79 0.1459584090793271 1103 80-81 0.002229841504327368 1103 82-83 0.13341341248564476 1103 84-85 0.1912258140873604 1103 86-87 0.11276181465724022 1103 88-89 0.09669027639514738 1103 90-91 0.11605638125482542 1103 92-93 0.29011366376771974 1103 94-95 -0.001523999803563214 1103 96-97 -0.12079007359372085 1103 98-99 -0.23800286918933722 1103 100-101 8.627483317305007E-4 1104 1 0.2291051010466063 1104 2 0.28188204280066387 1104 3 0.1394655835378238 1104 4 -0.041097370265084976 1104 5 -0.07692202837792905 1104 6 -0.03163723906517646 1104 7 0.027382940717558313 1104 8 -0.161380419902315 1104 9 -0.21035138028241107 1104 10-11 -0.19774503429037793 1104 12-13 -0.19733108484418693 1104 14-15 -0.13035597310148006 1104 16-17 -0.08547165630651676 1104 18-19 -0.06581762219627052 1104 20-21 -0.23976618016458673 1104 22-23 -0.2509366986719783 1104 24-25 -0.23344080512370624 1104 26-27 -0.21013175312871368 1104 28-29 -0.32667978818643206 1104 30-31 -0.19452182397366613 1104 32-33 -0.26432944201270914 1104 34-35 -0.16642542919114334 1104 36-37 -0.1704633769078967 1104 38-39 -0.08605343023030088 1104 40-41 0.04872784477289116 1104 42-43 0.16707983900722212 1104 44-45 0.013477182340288607 1104 46-47 -0.06439195110083062 1104 48-49 0.07046228260941945 1104 50-51 -0.026022224850812847 1104 52-53 -0.2676273081909528 1104 54-55 -0.0543589411856189 1104 56-57 -0.02189011480247416 1104 58-59 -0.07823552364786934 1104 60-61 -0.5153546684544992 1104 62-63 -0.7170733417306678 1104 64-65 -0.528546924127582 1104 66-67 -0.4422871344263122 1104 68-69 -0.5836051446308268 1104 70-71 -0.5330903939918379 1104 72-73 -0.3058460214353964 1104 74-75 -0.31648050341509304 1104 76-77 -0.33708912717231954 1104 78-79 -0.3208194277582983 1104 80-81 -0.3232266622504163 1104 82-83 -0.057253088991075174 1104 84-85 -0.16209750127490707 1104 86-87 -0.05388253804461485 1104 88-89 -0.2093101152648913 1104 90-91 -0.25397343286651974 1104 92-93 -0.024841954755402185 1104 94-95 -0.0547747781279746 1104 96-97 -0.19301738029150783 1104 98-99 -0.10207985036967315 1104 100-101 -0.13192499190974516 1105 1 0.28018501250974737 1105 2 0.388654814738846 1105 3 0.22277658414328627 1105 4 0.07684672843564755 1105 5 -0.024308602934503654 1105 6 0.08283784257335469 1105 7 0.05654203907089084 1105 8 0.07743088343568161 1105 9 0.005189220616856005 1105 10-11 0.028469369822815338 1105 12-13 -0.08803719607110594 1105 14-15 0.15121257844742075 1105 16-17 0.3564322335583938 1105 18-19 0.1605121676775738 1105 20-21 0.20833752309797404 1105 22-23 0.146935736786439 1105 24-25 -0.018056533319523282 1105 26-27 0.3293922123696973 1105 28-29 0.1777234813249322 1105 30-31 0.038341529508748806 1105 32-33 0.2185636884401987 1105 34-35 0.055690919358220015 1105 36-37 -0.10474188091289705 1105 38-39 0.06019988245766683 1105 40-41 0.176910258494118 1105 42-43 0.09631735004951025 1105 44-45 0.09307722799831453 1105 46-47 0.3445910164720445 1105 48-49 0.4133986889261152 1105 50-51 0.44457673891061944 1105 52-53 0.42662896234274683 1105 54-55 0.5083439144258506 1105 56-57 0.38127641786783073 1105 58-59 0.2799863941875529 1105 60-61 0.3594790767935976 1105 62-63 0.18134748626762587 1105 64-65 0.37925985311090216 1105 66-67 0.585228027014498 1105 68-69 0.05448803743549391 1105 70-71 0.2769362563967519 1105 72-73 0.21636263572249348 1105 74-75 0.2254020462876305 1105 76-77 0.25593288793453084 1105 78-79 0.2814086340586961 1105 80-81 0.2999048328512721 1105 82-83 0.43601731567795454 1105 84-85 0.4969326131679175 1105 86-87 -0.05113809393102642 1105 88-89 0.08275109626077892 1105 90-91 -0.1785123861067497 1105 92-93 0.15029253789464647 1105 94-95 0.20644989904003452 1105 96-97 0.1511187897945483 1105 98-99 0.31105328246539443 1105 100-101 0.5678884057418436 1106 1 0.2985149425235356 1106 2 0.3793916770527552 1106 3 0.07677417564022804 1106 4 -9.976613229767395E-4 1106 5 -0.09758032745759948 1106 6 -0.3252245341653932 1106 7 -0.004119950213016921 1106 8 0.060335747564693065 1106 9 -0.002673320179347627 1106 10-11 -0.03370011549055363 1106 12-13 -0.04554806051427107 1106 14-15 0.06997359560363492 1106 16-17 -0.057784479358510055 1106 18-19 -0.0833400409895475 1106 20-21 0.17591872281175114 1106 22-23 0.0764346634318187 1106 24-25 -0.14366084070036322 1106 26-27 9.500600755103505E-4 1106 28-29 -0.04429730510367591 1106 30-31 -0.2336404241711989 1106 32-33 -0.07250303407581526 1106 34-35 -0.34361445439606797 1106 36-37 -0.3205363378652706 1106 38-39 -0.040488198942753684 1106 40-41 -0.18936982597801943 1106 42-43 -0.3398180497096419 1106 44-45 -0.13661411709828997 1106 46-47 -0.13013933590242033 1106 48-49 -0.25290146640488587 1106 50-51 -0.21756307643747874 1106 52-53 -0.21740344773107978 1106 54-55 0.03388190070785413 1106 56-57 -0.3370618022833227 1106 58-59 -0.184091131686408 1106 60-61 -0.10955289695721149 1106 62-63 -0.34611468452665406 1106 64-65 -0.029974050932942475 1106 66-67 -0.08296358860237518 1106 68-69 -0.25027050140598206 1106 70-71 -0.30268178643271426 1106 72-73 0.1124174029877949 1106 74-75 0.12700029750497066 1106 76-77 0.05776588093590718 1106 78-79 0.11905065720126728 1106 80-81 0.13347159899958783 1106 82-83 0.14539564264097038 1106 84-85 0.053787684114038115 1106 86-87 -0.010241537392289501 1106 88-89 0.10992747547783921 1106 90-91 -0.013475385914766491 1106 92-93 0.13608804202226565 1106 94-95 0.3889136405971847 1106 96-97 0.5032685533074712 1106 98-99 0.27528352139587753 1106 100-101 0.3184032669038608 1107 1 0.39550785374066777 1107 2 0.5226999763970568 1107 3 0.11382598445880987 1107 4 0.17164154320822433 1107 5 0.01200984757964818 1107 6 0.04659542178682585 1107 7 0.13978033524469424 1107 8 0.24014400426592886 1107 9 0.46256505456793207 1107 10-11 0.37961029092296883 1107 12-13 0.37887086498076883 1107 14-15 0.47465254347374497 1107 16-17 0.4673097116523337 1107 18-19 0.2776122037916906 1107 20-21 0.15993509774966697 1107 22-23 0.3858163891215085 1107 24-25 0.3480551163354946 1107 26-27 0.2566792816137706 1107 28-29 0.20632729041428632 1107 30-31 0.4329785825965047 1107 32-33 0.5350089185251576 1107 34-35 0.4165897808130552 1107 36-37 0.5639799643285954 1107 38-39 0.6736101008530611 1107 40-41 0.7954881224389183 1107 42-43 0.7892712950420062 1107 44-45 0.6292127319803669 1107 46-47 0.9434462938855148 1107 48-49 0.7837489958961399 1107 50-51 0.7629678548369441 1107 52-53 0.513186431313116 1107 54-55 0.8470563509885451 1107 56-57 1.0255309463884785 1107 58-59 0.9079539294938499 1107 60-61 0.7995082532015729 1107 62-63 0.8606778544415832 1107 64-65 0.9356192646408132 1107 66-67 1.2044782788629753 1107 68-69 0.9071614327330586 1107 70-71 0.884915101125042 1107 72-73 0.9861826353423453 1107 74-75 0.9597090353203441 1107 76-77 1.0078835256338685 1107 78-79 0.9553765305062036 1107 80-81 1.2097311999608138 1107 82-83 1.0608106098559809 1107 84-85 0.9720685163060452 1107 86-87 1.4113792503505884 1107 88-89 1.095882855778754 1107 90-91 1.305233883684739 1107 92-93 1.3496733708003141 1107 94-95 1.588382859827707 1107 96-97 1.5582793645792883 1107 98-99 1.4781428644919643 1107 100-101 1.3082278784756873 1108 1 0.09416446029200998 1108 2 0.24886854467088781 1108 3 -0.08805108583931087 1108 4 0.09803094328183448 1108 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-1.0052687348393832 2316 50-51 -1.0723823777525539 2316 52-53 -1.2535507869094147 2316 54-55 -0.9396168563336786 2316 56-57 -0.9361645263660847 2316 58-59 -0.9113651918110648 2316 60-61 -0.9308112335757812 2316 62-63 -1.017049439252773 2316 64-65 -0.9164114137540906 2316 66-67 -1.1528934420864054 2316 68-69 -1.4617803294118055 2316 70-71 -1.1243441252798085 2316 72-73 -1.185489170410829 2316 74-75 -1.450767959431758 2316 76-77 -1.5086088615398232 2316 78-79 -0.9608717352738658 2316 80-81 -0.8623493671956339 2316 82-83 -0.8304125518708965 2316 84-85 -1.3608804204530323 2316 86-87 -1.1023605616617438 2316 88-89 -1.1026059334430052 2316 90-91 -1.1839350036113672 2316 92-93 -1.4481627130383536 2316 94-95 -1.7139657441030742 2316 96-97 -1.6541869711514963 2316 98-99 -1.9606999362333042 2316 100-101 -2.752801582860524 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 25.0 5 31.0 6 88.0 7 182.0 8 408.0 9 744.0 10 1298.0 11 2059.0 12 3108.0 13 4029.0 14 5195.0 15 6919.0 16 8862.0 17 11262.0 18 9807.0 19 6281.0 20 5557.0 21 5899.0 22 6100.0 23 6685.0 24 7685.0 25 9457.0 26 11457.0 27 13794.0 28 17079.0 29 21026.0 30 25650.0 31 31804.0 32 38933.0 33 48890.0 34 63698.0 35 85476.0 36 117671.0 37 146728.0 38 89857.0 39 5490.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.41035455675473 18.832868022808434 14.774307953357141 21.98246946707969 2 15.727776048247783 21.057495681261866 39.040171894934105 24.174556375556246 3 20.749378041128633 24.667657894544096 30.328277094579253 24.25468696974802 4 12.925890114612482 17.015094468617082 37.54340087021492 32.51561454655552 5 13.322640456912957 37.880491237578376 33.81109495786934 14.985773347639327 6 31.00177360153411 36.314277797511586 16.987147797279665 15.696800803674638 7 27.9272054362779 33.96684964180084 20.229066802402723 17.876878119518526 8 26.724745098182822 32.61107591576064 19.548799675795895 21.115379310260646 9 26.16015264025899 17.33340983250344 20.907101116847194 35.59933641039038 10-11 25.38670270032738 26.11543222083068 26.441956754724533 22.055908324117407 12-13 27.41234200198966 23.856280554419673 27.118287182554457 21.61309026103621 14-15 23.534915457816798 24.974671523224075 27.31520587474183 24.175207144217296 16-17 21.640380012133242 28.414793277159113 27.820520801672778 22.12430590903487 18-19 21.388695449970797 26.82485460547717 30.562786203983833 21.2236637405682 20-21 22.415735617409858 25.07235390979655 30.45120032420411 22.06071014858948 22-23 21.76969480949091 25.147273157834345 30.838498778114953 22.24453325455979 24-25 22.654799526876836 25.235920740884506 30.73555027421857 21.373729458020087 26-27 22.065836870160894 25.126001389097418 31.51639879546456 21.291762945277128 28-29 21.138266460133025 26.418301419491552 30.985477950824976 21.457954169550447 30-31 21.519963087604683 25.541693063179522 30.71040492555878 22.22793892365701 32-33 21.56777773030512 25.6543393738899 30.753736945866926 22.02414594993805 34-35 22.703636341442575 25.222526030540877 30.4053196294081 21.66851799860845 36-37 22.495158377365193 24.851363863581295 31.04992257676253 21.603555182290986 38-39 21.93508460021569 25.65165938243643 30.956422176529212 21.456833840818664 40-41 21.70556593506867 25.98712018052011 30.695438229418503 21.611875654992716 42-43 21.54253062579849 26.02300147169864 29.816455800513108 22.618012101989756 44-45 22.158956702825463 25.61728639952735 29.48540088292325 22.73835601472393 46-47 22.80697792512805 26.36027060150486 29.07434217272208 21.75840930064501 48-49 22.810082416811028 26.44571245798891 28.635660561401195 22.108544563798866 50-51 21.963775873286355 26.740744086236763 29.47500894129021 21.820471099186676 52-53 22.662104542308494 27.251958901818707 27.986936178679677 22.09900037719312 54-55 21.78525385903828 26.565346055992066 28.63202525649603 23.01737482847362 56-57 22.764454915556314 26.527341198874492 28.07711322839163 22.63109065717756 58-59 23.52857524752113 26.103820327310146 28.013190547628668 22.354413877540058 60-61 22.59009817349926 26.136100063235357 29.582060691291833 21.691741071973553 62-63 22.031498244909447 26.664619925694762 29.25883358958286 22.045048239812935 64-65 21.82414996987343 27.28276668728012 28.532210398824482 22.360872944021967 66-67 22.05157470703125 26.78253173828125 27.997680664062504 23.168212890625 68-69 22.1081047100735 26.507155774269485 28.541435378313995 22.843304137343015 70-71 22.828633124964142 26.63860335270544 28.045431075206995 22.487332447123418 72-73 22.426185844991036 26.298327772261125 28.838192634239316 22.43729374850852 74-75 21.98997359738391 27.43381951742902 28.029495738107528 22.546711147079538 76-77 22.146622038958917 26.716506636784658 28.07236622063372 23.064505103622707 78-79 22.417792373476253 26.665486531195253 28.153555971994166 22.763165123334332 80-81 22.707053047261443 26.968036368009386 27.731400738519163 22.593509846210008 82-83 22.440979248502163 26.60336528923602 28.370676262593893 22.584979199667927 84-85 22.07349550778043 27.005079116657317 28.19999304146979 22.721432334092466 86-87 22.006196916923955 27.354211913560576 27.78778855767857 22.851802611836895 88-89 21.64192408476054 27.201007123616854 27.14520505124755 24.01186374037506 90-91 21.890581281979117 26.569323369045954 27.75743093792793 23.782664411047 92-93 22.32665305586836 26.63303639820435 28.196508189367098 22.843802356560193 94-95 21.80486512618173 26.529782600203138 28.204229822642397 23.46112245097273 96-97 21.217061891414115 26.491371143384544 28.826216683432794 23.46535028176855 98-99 21.484712158854375 26.94206552193526 27.561575602937317 24.01164671627305 100-101 20.888317529382896 26.354689726150323 28.00491759848321 24.752075145983575 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 235.0 1 191.0 2 251.5 3 314.0 4 223.0 5 187.5 6 326.0 7 594.0 8 983.5 9 1245.5 10 1131.5 11 962.0 12 965.0 13 1092.0 14 1254.0 15 1395.5 16 1487.5 17 1561.0 18 1697.5 19 1857.0 20 2008.5 21 2192.5 22 2506.5 23 2933.0 24 3398.5 25 4003.5 26 4810.0 27 5895.0 28 6951.5 29 8214.0 30 9626.0 31 11066.0 32 12955.0 33 14225.5 34 15298.0 35 16485.0 36 17906.5 37 19360.5 38 20447.0 39 20906.0 40 21256.5 41 22419.5 42 23602.0 43 24871.0 44 25737.5 45 26981.0 46 28676.0 47 34382.0 48 46314.0 49 44264.5 50 32588.5 51 28408.0 52 26584.0 53 26152.5 54 25232.0 55 23419.5 56 21267.0 57 19424.0 58 18310.5 59 16309.5 60 13621.0 61 11317.5 62 9499.5 63 7344.5 64 5119.5 65 3889.5 66 2979.5 67 2392.5 68 1828.0 69 1205.5 70 929.0 71 754.5 72 636.0 73 562.5 74 481.0 75 427.5 76 323.5 77 213.0 78 145.5 79 100.0 80 78.5 81 58.0 82 40.5 83 28.5 84 18.0 85 11.0 86 5.5 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0142364175446177 2 0.0161125535101564 3 0.0062253047652877005 4 0.0157463591121983 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0068356287618845345 10-11 3.6619439795810004E-4 12-13 2.4412959863873336E-4 14-15 1.2206479931936668E-4 16-17 0.0 18-19 6.103239965968334E-5 20-21 0.0 22-23 0.0013427127925130335 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.006347369564607068 34-35 8.544535952355668E-4 36-37 0.004699494773795617 38-39 1.8309719897905002E-4 40-41 0.0059201427669892835 42-43 0.014098484321386853 44-45 0.0032347171819632173 46-47 0.003051619982984167 48-49 0.013182998326491602 50-51 4.882591972774667E-4 52-53 0.0034178143809422672 54-55 0.01476984071764337 56-57 0.006347369564607068 58-59 0.0017089071904711336 60-61 0.009154859948952501 62-63 0.0059201427669892835 64-65 0.023497473868978085 66-67 0.004516397574816567 68-69 4.272267976177834E-4 70-71 0.0021971663877486 72-73 6.103239965968334E-5 74-75 0.0 76-77 0.0018309719897905 78-79 0.014891905516962735 80-81 0.020201724287355186 82-83 0.01702803950505165 84-85 0.012023382732957619 86-87 0.014647775918324 88-89 0.03307956061554837 90-91 0.02996690823290452 92-93 0.00421123557651815 94-95 0.012328544731256033 96-97 0.015258099914920834 98-99 0.014891905516962735 100-101 0.0168449423060726 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 819237.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.80494617989235 #Duplication Level Percentage of deduplicated Percentage of total 1 83.63089346028968 50.851719758292255 2 9.987777685942811 12.14612569300965 3 2.63567890233902 4.807869414126055 4 1.1755299778449755 2.8591214814285513 5 0.6169081341271653 1.8755532946770046 6 0.36448520853649335 1.329750209305699 7 0.256922473874544 1.0935510037442513 8 0.18842370502083147 0.9165674594246056 9 0.15096360762809474 0.8261400633253819 >10 0.8795485087975387 10.06461850124797 >50 0.0675599426731297 2.790827918585804 >100 0.039442574206333264 4.571781611640784 >500 0.0028317745541701737 1.1745538134907878 >1k 0.0028317745541701737 3.9287902636806 >5k 2.0226961101215524E-4 0.7630295140206123 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6204 0.7572900149773509 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4699 0.5735824920017041 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4171 0.5091322779610784 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3859 0.47104806057343596 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2872 0.3505701036452211 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2529 0.30870187747867833 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2326 0.2839227232168469 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2206 0.2692749472985229 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1930 0.23558506268637772 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1433 0.17491885742465246 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1254 0.15306925834648583 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1214 0.14818666637371117 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1199 0.14635569438392065 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1126 0.13744496403360687 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1126 0.13744496403360687 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 901 0.10998038418674938 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 876 0.10692876420376521 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 820 0.10009313544188068 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.2206479931936668E-4 0.0 14-15 0.0 0.0 0.0 1.2206479931936668E-4 0.0 16-17 0.0 0.0 0.0 1.2206479931936668E-4 6.103239965968334E-5 18-19 0.0 0.0 0.0 2.4412959863873336E-4 1.2206479931936668E-4 20-21 0.0 0.0 0.0 3.6619439795810004E-4 1.2206479931936668E-4 22-23 0.0 0.0 0.0 4.272267976177834E-4 1.8309719897905002E-4 24-25 0.0 0.0 0.0 6.713563962565167E-4 3.6619439795810004E-4 26-27 0.0 0.0 0.0 0.0010375507942146167 3.6619439795810004E-4 28-29 0.0 0.0 0.0 0.0020751015884292335 3.6619439795810004E-4 30-31 0.0 0.0 0.0 0.0033567819812825835 3.6619439795810004E-4 32-33 0.0 0.0 0.0 0.007568017557800734 3.6619439795810004E-4 34-35 0.0 6.103239965968334E-5 0.0 0.010070345943847751 3.6619439795810004E-4 36-37 0.0 1.2206479931936668E-4 0.0 0.013305063125810968 3.6619439795810004E-4 38-39 0.0 1.2206479931936668E-4 0.0 0.021849599078166637 4.882591972774667E-4 40-41 0.0 1.2206479931936668E-4 0.0 0.03307956061554837 4.882591972774667E-4 42-43 0.0 1.2206479931936668E-4 0.0 0.05425780329745849 4.882591972774667E-4 44-45 0.0 1.2206479931936668E-4 0.0 0.07537501357970892 4.882591972774667E-4 46-47 0.0 1.2206479931936668E-4 0.0 0.11791459614250821 4.882591972774667E-4 48-49 0.0 1.2206479931936668E-4 0.0 0.15813494751823953 4.882591972774667E-4 50-51 0.0 1.2206479931936668E-4 0.0 0.1871863697562488 4.882591972774667E-4 52-53 0.0 1.2206479931936668E-4 0.0 0.22331755035478135 5.4929159693715E-4 54-55 0.0 1.8309719897905002E-4 0.0 0.26402616092779013 6.103239965968333E-4 56-57 0.0 2.4412959863873336E-4 0.0 0.34379550728299624 6.103239965968333E-4 58-59 0.0 3.6619439795810004E-4 0.0 0.4291798344068932 6.103239965968333E-4 60-61 0.0 3.6619439795810004E-4 0.0 0.520972563495057 6.103239965968333E-4 62-63 0.0 3.6619439795810004E-4 0.0 0.6116056769896867 6.103239965968333E-4 64-65 0.0 3.6619439795810004E-4 0.0 0.7095016460438188 6.103239965968333E-4 66-67 0.0 3.6619439795810004E-4 0.0 0.8579934744158284 6.103239965968333E-4 68-69 0.0 3.6619439795810004E-4 0.0 1.049452112148255 6.103239965968333E-4 70-71 1.2206479931936668E-4 3.6619439795810004E-4 0.0 1.1896435341665477 6.103239965968333E-4 72-73 1.2206479931936668E-4 3.6619439795810004E-4 0.0 1.3092060050998673 7.323887959162001E-4 74-75 1.2206479931936668E-4 3.6619439795810004E-4 0.0 1.4481767791249662 7.323887959162001E-4 76-77 1.2206479931936668E-4 3.6619439795810004E-4 0.0 1.6136356146023678 7.323887959162001E-4 78-79 1.2206479931936668E-4 3.6619439795810004E-4 0.0 1.7894089256222558 7.323887959162001E-4 80-81 1.2206479931936668E-4 3.6619439795810004E-4 0.0 2.065763631281302 7.323887959162001E-4 82-83 1.2206479931936668E-4 3.6619439795810004E-4 0.0 2.3709256295797188 7.323887959162001E-4 84-85 1.2206479931936668E-4 3.6619439795810004E-4 0.0 2.76385221858876 7.323887959162001E-4 86-87 1.2206479931936668E-4 3.6619439795810004E-4 0.0 3.1623937883664923 7.323887959162001E-4 88-89 1.2206479931936668E-4 3.6619439795810004E-4 0.0 3.6295968077613683 7.323887959162001E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCTACG 95 0.001153219 25.264668 1 GTACATG 4160 0.0 23.770653 1 TACATGG 4170 0.0 23.355026 2 ACATGGG 4125 0.0 23.258617 3 GTACACG 125 2.0998825E-4 23.041378 1 GAGTACT 4835 0.0 22.541857 12-13 GTACTTT 5120 0.0 21.19433 14-15 AGTACTT 4890 0.0 21.025799 12-13 CATGGGA 2515 0.0 20.961143 4 CCTATAA 425 0.0 20.330627 1 GTGTAGG 290 2.1354936E-9 19.863256 1 CATGGGG 1650 0.0 19.285051 4 TACCTGG 600 0.0 19.003016 2 ATGGGAT 1100 0.0 18.996058 5 GGGCGTA 125 0.006036927 18.996058 9 GAGTACG 130 0.007115636 18.462643 1 ACTTTTT 5995 0.0 18.18013 16-17 AGAGTAC 8085 0.0 18.032743 10-11 ATGGGTA 320 8.281859E-9 17.808804 5 GTAAGGT 295 6.34227E-8 17.709269 4 >>END_MODULE