FastQCFastQC Report
Thu 26 May 2016
SRR936449_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936449_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831656
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT63470.7631761208961397No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT35530.42721990823128797No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32100.3859768942928326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27020.32489394653558684No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24680.29675731311984765No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC24230.2913464220783593No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT18990.22833960195080658No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC15200.1827678751791606No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT14800.17795819425339324No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14480.17411044951277932No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC13550.16292794136037017No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG12220.1469357522821936No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC11300.13587348615292863No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA11270.13551276008349605No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10960.13178525736602634No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA10220.12288734765335668No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA10220.12288734765335668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG45600.024.8067761
TACATGG46350.024.0977442
GAGTACT31850.023.43576612-13
ACATGGG47400.022.460943
GTACTAT1553.8864386E-521.464691
GTACTTT34900.021.2501514-15
AGTACTT33500.019.44306612-13
CTAGACT2251.6558515E-619.0127264
CTATACT3506.366463E-1119.0127264
ACTATAC2251.6568065E-619.0115813
GTGTAAG4001.8189894E-1219.0115811
CATGGGA27750.018.841444
CATATAC3054.5220077E-918.6999173
ACTTTTT41700.018.69188116-17
TACCTGG5600.018.6720892
ACTAGAC2352.511044E-618.2025783
TCGCGCT1051.9615956E-518.08993560-61
CATGGGG19250.018.0250514
GTGTAGC4002.1827873E-1117.8233571
GTATAAA4103.274181E-1117.3886431
Produced by FastQC (version 0.11.5)